胃癌术后化疗对外周血免疫细胞比例及凋亡的影响

目的探讨胃癌患者术后及化疗中外周血T淋巴细胞亚群及NK细胞动态变化和淋巴细胞凋亡情况及其意义.方法以流式细胞术检测64例胃腺癌术前后及化疗期间外周血T淋巴细胞亚群及NK细胞变化,并以Annexin-V/PI双标记流式细胞术检测淋巴细胞凋亡及坏死情况.结果胃癌患者根治术后3d外周血T淋巴细胞亚群及NK细胞数量较术前轻微下降,统计分析未见显著性差异.至化疗前1d,患者外周血CD3、CD4及NK增高显著(P<0.05),而CD8下降非常显著(P<0.01).化疗开始后CD3、CD4和CD8降低明显,但CD4/CD8改变不明显,而且NK细胞降低迟于CD3等,恢复也较快.淋巴细胞凋亡及坏死在化疗期间增加明显,化疗结束3d后方下降.结论胃癌根治手术本身对细胞免疫功能影响轻微,术后细胞免疫功能异常情况得到明显改善.化疗对淋巴细胞数量有较明显一过性抑制,可能与化疗药物致淋巴细胞凋亡和坏死有关,但对淋巴亚群比例影响不大.     

南京地区健康成年人外周血淋巴细胞亚群正常值调查

目的 :建立健康成年人外周血淋巴细胞亚群正常参考值。方法 :使用美国Becton Dickinson(B D)公司生产的双标荧光抗体 ,分别对 2 98例健康成年人外周血淋巴细胞亚群进行标记 ,用B D公司的FACSCaliburTM型流式细胞仪进行检测并分析结果 ,SPSS 10 0软件进行统计学处理。结果 :18～ 5 7岁健康成年人外周血中CD3 + 、CD3 -、CD19+ 、CD3 +CD4+ 、CD3 + CD8+ 及CD3 -CD(16+ 56) + 细胞数分别为 6 6 6± 8 7、11 3± 4 1、35 9± 6 7、2 9 7± 7 9及 2 1 2± 9 1。CD3 +CD4+ 与CD3 + CD8+ 的比率为 1 35± 0 75。男女之间CD3 + 、CD3 + CD4+ 及CD3 -CD(16+ 56) + 细胞数有显著性差异 (P <0 0 1)。结论 :在相同地域、相同种族 ,可以建立健康成年入外周血淋巴细胞亚群正常参考值。     

T淋巴细胞亚群异常与特发性血小板减少性紫癜的关系

目的通过检测T淋巴细胞亚群及其凋亡,初步探讨T淋巴细胞亚群异常在特发性减少性紫癜(ITP)发病机制中的作用.方法采用流式细胞术对ITP患者及正常对照组的外周血淋巴细胞亚群进行检测;DNA琼脂糖凝胶电泳检测T淋巴细胞的凋亡.结果 ITP患者CD+3、CD+4和CD+4CD+25T细胞比例均显著低于正常对照组(P<0.05或P<0.01),而CD+8T细胞和CD+4T细胞表面Fas 表达均较正常组增加(P<0.01);T淋巴细胞凋亡敏感性增加.结论 ITP患者外周血T淋巴细胞亚群异常,T细胞免疫调控亚群(CD+4CD+25)凋亡增多,对自身反应性T细胞亚群免疫下调作用降低,破坏自身免疫耐受,在ITP的发病中起重要作用.     

医学生外周血T细胞mIL-2R的表达

目的:测定正常医学生外周血T细胞数量及其亚群比例和活化后mIL-2R的表达水平,为正常机体细胞免疫功能状态提供参考数据.方法:用生物素-链霉亲和素法对49名在校医学生外周血T淋巴细胞亚群及mIL-2R进行检测.结果:男生外周血淋巴细胞中总T细胞亚群(CD3+)占(64.90±4.11)%,其中CD4+和CD8+ T细胞亚群分别为(42.84±4.08)%和(30.23±2.03)%,CD4+/CD8 +比例为1.42±0.10;植物血凝素(PHA)诱导前后淋巴细胞的mIL-2R表达水平分别为(3.08± 1.22)%和(3 1.76±3.52)%;女生T细胞亚群阳性百分率分别为CD3+(66.61±3.36)%、CD4+(43.83± 3.85)%、CD8+(29.06±2.53)%、CD4+/CD8+1.52±0.16,PHA诱导前后的mI L- 2R表达水平分别为(3.48±1.41)%和(34.58±3.05)%.虽然女生CD3+、CD4+和mIL- 2R表达水平偏高,CD8+偏低,但差异均无显著性(P＞0.05);女生CD4+/CD8+ 比值及诱导期mIL-2R表达水平较男生增高(P＜0.05和P＜0.01). 结论:医学生外周血T淋巴细胞亚群的百分率均在正常范围,男生和女生之间无明显差异,但女生CD4+/CD8+比值较高;PHA刺激诱导mIL-2R正常表达,但在女生较高.     

Apo2.7抗体原位标记法在原发性肝癌病人外周血淋巴细胞凋亡检测中的应用价值

目的　探讨Apo2.7抗体原位检测细胞凋亡的应用价值。方法　采集42例原发性肝癌病人和37例健康人的 外周血,分离外周血淋巴细胞后,分别采用Apo2.7抗体原位检测法和流式细胞仪检测细胞凋亡,并将结果进行比较。结果　 原发性肝癌病人Apo2.7抗体原位检测外周血淋巴细胞凋亡率为(12.4±1.2)%,流式细胞仪检测细胞凋亡率为(12.1± 0.7)%,2种方法检测外周血淋巴细胞凋亡率无显著性差异(t=1.43,P>0.05),但均明显高于对照组(P<0.01)。结论　 Apo2.7抗体原位检测法可替代流式细胞仪用于检测外周血淋巴细胞的凋亡;原发性肝癌病人淋巴细胞凋亡率高于正常人。     

白介素-9产生细胞在不同淋巴细胞亚群中的比例

目的:应用流式细胞术测定胞内细胞因子,检测不同T淋巴细胞亚群和其他淋巴细胞亚群中白介素-9(IL-9)产生细胞的比例。方法:23名健康成年人外周抗凝血加等量1640培养液后,加佛波酯、离子霉素及莫能霉素培养6 h。收集细胞后,先用不同荧光素标记的抗人CD3、抗人CD8和抗人T细胞抗原受体γδ(TCRγδ)单抗,或抗人CD19和抗CD56单抗作细胞表面染色,再经过溶血,固定和透膜处理后,加抗胞内细胞因子的抗IL-9和IL-17的荧光抗体作胞内染色,用流式细胞仪检测和分析IL-9产生细胞在不同T淋巴细胞亚群和其他淋巴细胞亚群中的分布频率。结果:IL-9产生细胞在总T细胞(CD3+T)、CD4+T细胞、CD8+T细胞和γδT细胞中的百分比分别为0.35±0.31、0.22±0.13、0.31±0.29和1.07±1.05。γδT细胞中的IL-9产生细胞明显高于其他淋巴细胞亚群(P<0.01)。同时也发现自然杀伤细胞(NK细胞)中也有少量IL-9产生细胞。另外还发现,共表达IL-9和IL-17的细胞在CD3+T及其亚群CD4+T和CD8+T细胞中的比例很低(0.03%~0.06%),但在γδT细胞中的比例可达(0.28±0.34)%,差异有统计学意义(P<0.01)。结论:用流式细胞术测定单细胞水平胞内细胞因子的技术发现,不仅CD4+T细胞中,而且CD8+T细胞和γδT细胞以及NK细胞中均存在产生IL-9的细胞。     

淋巴细胞检查在慢性阻塞性肺疾病(COPD)中的临床应用

目的:探讨慢性阻塞性肺疾病慢(COPD)患者T淋巴细胞亚群的变化及其临床意义。方法:将100例COPD患者及30例门诊体检健康者进行T细胞亚群检测。T淋巴细胞亚群(CD3+、CD4+、CD8+细胞)比例采用直接免疫荧光标记的流式细胞分析测定。结果:100例COPD患者组外周血中,CD3+细胞比例(68.23±7.32),与30例对照组CD3+细胞比例(67.57±4.85)差异无统计学意义(P>0.05)。CD4+细胞比例(35.77±4.71)及CD4+/CD8+比值(1.25±0.36)都有降低,与30例对照组比较CD4+细胞比例(40.85±3.58),CD4+/CD8+比值(2.09±0.45)差异有统计学意义(P<0.001)。而CD8+细胞比例(25.32±6.15)提高,和对照组(20.28±3.20)比较,差异有统计学意义(P<0.001)。结论:COPD患者存在细胞免疫功能异常,CD4+T淋巴细胞比及CD4+/CD8+比值下降,而CD8+T淋巴细胞免疫反应加强,是COPD发病机制之一。     

HIV感染和AIDS患者T淋巴细胞免疫病理改变的研究

目的　探讨我国HIV感染和AIDS患者T淋巴细胞的免疫病理改变情况及其临床意义。方法　收集 13例HIV感染患者 (HIV组 )、2 7例AIDS患者 (AIDS组 )和 5 1名健康献血员 (正常对照组 )的抗凝血 ,用人T淋巴细胞亚群的特异性荧光抗体标记 ,通过流式细胞仪检测外周血CD4 + T淋巴细胞 (T4细胞 )、CD8+ T淋巴细胞 (T8细胞 )及其亚群 ,并检测患者血浆HIV载量。结果　正常对照组、HIV组和AIDS组外周血T4细胞计数 (× 10 6个 /L)分别为 84 9± 2 88,4 37± 184 ,5 0± 5 1,组间比较P <0 0 1;T8细胞计数 (× 10 6个 /L)分别为 5 79± 175 ,10 31± 345 ,5 35± 338,HIV组明显高于正常对照组(P <0 0 5 ) ;T4纯真细胞 (CD4 + CD4 5RA+ CD6 2L+ )亚群比例分别为 4 3 0 %± 11 4 % ,4 4 2 %± 12 8% ,2 4 8%± 15 5 % ,AIDS组明显为低 (P <0 0 5 ) ;T4纯真细胞计数 (× 10 6个 /L)分别为 36 8± 16 2 ,185±134,18± 2 0 ,组间比较P <0 0 1;T4功能细胞 (CD4 + CD2 8+ )亚群比例分别为 93 1%± 8 1% ,6 2 6 %±2 8 2 % ,5 6 9%± 2 6 4 % ,组间比较P <0 0 1;T4和T8激活细胞 (CD4 + HLA DR+ ,CD8+ HLA DR+ ,CD8+CD38+ )亚群比例均表现为AIDS组 >HIV组 >正常对照组 (P <0 0 5 ) ;T4和T8凋亡细胞 (CD4 +CD95 + ,C     

Graves病患者外周血CD4+CD28-T淋巴细胞的检测和分选

目的 分析CD4^＋CD28ˉT淋巴细胞Graves病（毒性弥漫性甲状腺肿）患者外周血的表达及探索纯化该T细胞的有效方法。方法 运用常规密度梯度法分离Graves病患者外周血单个核细胞，用CD4、CD28单克隆抗体进行免疫荧光双标记后进行流式细胞术检测和分选。结果 Grave病患者外周血T细胞亚群中，CD4^＋CD28ˉ的表达率明显高于正常人（P〈0．01），采用流式细胞术和单克隆抗体双标记对CD4^＋CD28ˉT淋巴细胞进行快速无菌分选是可行的。结论 流式细胞术可用于Graves病患者外周血CD4^＋CD28ˉT淋巴细胞的检测和分选。     

SARS患者T淋巴细胞亚群变化的初步研究

目的探讨严重急性呼吸综合征(SARS)患者T淋巴细胞亚群的变化.方法采用流式细胞术对30例SARS患者进行外周血T淋巴细胞亚群进行测定.结果SARS患者在病程早期外周血白细胞(4153.25±515.61)μl和淋巴细胞(1 535.14±147.91)μl,CD3+(941.45±101.64)μl,CD4+(378.35±42.51)μl,CD8+(436.39±52.41)μl及CD4+/CD8+比值(0.95±0.54),较正常人外周血白细胞(6958.52±789.45)μl和淋巴细胞(2589.63±351.81)μl,CD3+(1 465.28±139.23)μl,CD4+(798.65±69.35)μl,CD8+(563.89±71.81)μl及CD4+/CD8+比值(1.49±0.64)均明显降低(P均＜0.05).结论SARS患者外周血T淋巴细胞亚群的变化对阐明SARS的发病机制及指导临床合理用药有一定意义.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.