The Effect of Xuefu Zhuyu Decoction(血府逐瘀汤) on in vitro Endothelial Progenitor Cell Tube Formation

<正>Objective:To observe the effect of Xuefu Zhuyu Decoction(血府逐瘀汤)-containing serum (XFZYD-CS) on endothelial progenitor cell(EPC) tube formation in vitro.Methods:Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge.EPCs were separated by the differential attachment method,and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation. Results:After one day,EPCs exposed to the serum containing 5%,10%and 15%XFZYD-CS formed typical tubes or vessel networks.The tube formation time was two days ahead of the control group and the size of most tubes in the serum groups was smaller than in the control group.Conclusion:XFZYD-CS could induce EPC angiogenesis and hasten tube formation,especially in capillary vessels.The study provides experimental evidence for the plausibility of Xuefu Zhuyu Decoction in the treatment of ischemic diseases.     

中医生新理论浅探

<正>人在其生命周期中,诸多因素均会造成机体不同程度的损伤。生新是人体维持正常生理功能、修复创伤、抵御疾病的重要途径。由此,人体的生新功能对于创伤修复以及诸多慢性疾病的预防、治疗、康复有着极为重要的意义。     

血府逐瘀汤诱导内皮祖细胞参与缺血区血管新生的实验研究

目的探讨血府逐瘀汤影响缺血区血管生长和修复损伤的作用。方法采用血管栓塞法制作下肢缺血大鼠模型,将DAPI荧光标记的内皮祖细胞由尾静脉注射植入模型鼠体内,继而给予不同剂量血府逐瘀汤灌胃,于给药第3天和第7天,分别采血并取材缺血坏死部位肉芽组织和肌肉组织,行冰冻切片分析荧光表达,常规病理切片观察各组样本坏死病变情况并计数局部血管数量。硝酸还原酶法检测血清NO水平。缺血下肢大体观察延续至30天。结果给药3天和7天,高剂量药物组缺血区荧光强度和肉芽组织血管数量均明显高于常规剂量药物组和生理盐水组,给药7天后常规剂量药物组的上述指标也显著高于生理盐水组;另外高剂量及常规剂量药物组两个时相点血清NO含量均明显高于生理盐水组。给药30天,高剂量药物组造模下肢肌肉萎缩现象最不显著。结论血府逐瘀汤通过提高NO水平诱导内皮祖细胞迁移至缺血区,促进血管新生,进而改善缺血坏死。     

缺氧对血府逐瘀胶囊促血管新生影响的研究

目的研究血府逐瘀胶囊在缺氧条件下对血管新生的影响。方法通过观察血府逐瘀胶囊含药血清在缺氧条件下对HMEC-1细胞的活力、增殖能力、黏附能力、迁移能力和体外成血管能力的影响,明确缺氧条件对药物促血管新生作用的影响。结果缺氧条件下含药血清在低浓度早、中期增加HMEC-1细胞活力,提高迁移及黏附能力;中、高浓度含药血清早期促进血管管腔形成,随后促管腔形成作用消失。结论缺氧条件下血府逐瘀胶囊通过影响HMEC-1细胞活力、迁移、黏附能力等环节来保护血管内皮细胞,促进血管新生。     

氧化-抗氧化状态与临床疾病的相关性

目的机体的氧化能力、自由基清除能力以及氧化-抗氧化之间的平衡与机体的健康状态关系密切。自由基具有活泼的化学性质,能和细胞内的有机物质发生链式反应,但产生过多会损伤细胞,使机体处于亚健康状态,且会导致机体出现癌症、心血管疾病、免疫性疾病等多种临床疾病。定量检测体内氧化-抗氧化状态,对评估机体的健康状况有重要意义。     

类脂质蛋白沉积症一家系调查

类脂质蛋白沉积症（lipoid proteinosis,LP）是一种罕见的常染色体隐性遗传病,主要特征包括皮肤和粘膜浸润以及透明物质沉积造成的疤痕。我们实地调查了山东省一LP家系的临床特点及发病情况,现报告如下。 1病例资料 先证者,女,19岁,声音嘶哑19年伴有睑缘丘疹（串珠样睑变形）,于2010年11月6日就诊。患者系第一胎、第一产,足月顺产,出生后数日即出现哭声嘶哑,多次给予抗炎等治疗无好转。     

A microarray analysis of angiogenesis modulation effect of Xuefu Zhuyu Decoction (血府逐瘀汤) on endothelial cells

Objective:To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction(血府逐瘀汤) on the endothelial cell line ECV304.Methods:ECV304 cells were treated with 2.5%Xuefu Zhuyu Decoctioncontaining serum(XFZYD-CS) for 24 h,48 h or 72 h.Thiazolyl blue tetrazolium bromide(MTT),fluorescence activating cell sorter(FACS),migration,adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points.An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction(RT-PCR) superarray.Results:At 48 h,XFZYD-CS induced ECV304 significantly improved cell viability,number in S phase,migration,adhesion and tube formation.At 24 h and 72 h, only cell migration was elevated.Microarray results showed that the expression of 27 angiogenesis-related genes was changed.Conclusion:XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.     

中医院校生物化学试题库建设与思考

建设试题库,施行教考分离是医学教育改革的重要途径之一.根据中医院校的专业特点和具体学情,通过培训教师、确定命题指导思想、制定命题方案、编写试题、制作软件、测试系统、修改完善等工作环节,有针对性地建设中医药特色生物化学试题库,促进中医院校考试管理和教学改革的科学化、规范化和标准化.