急性脑梗死患者中性粒细胞膜脂流动性及其影响因素的初步探讨

目的　探讨中性粒细胞（ＰＭＮ）膜脂流动性改变与脂质过氧化的关系。方法　采用荧光偏振技术对３６ 例急性脑梗死患者和２４ 例正常对照组外周血ＰＭＮ 膜脂流动性进行了测定。结果　脑梗死组ＰＭＮ 膜脂流动性较对照组显著下降（Ｐ＜ ０．００１），膜脂流动性改变与血浆ＭＤＡ 水平呈负相关，与血浆ＳＯＤ 水平呈正相关。结论　ＰＭＮ 膜脂流动性降低在脑梗死的病理过程中起着一定的作用。     

腹部横切口保留腹壁浅静脉在子宫切除术中的应用

1997年以来 ,我院在施行部分子宫切除术中采用腹部横切口钝性分离 ,保留腹壁浅静脉 ,取得了良好的临床效果 ,现报道如下。1　临床资料与方法1.1　临床资料 :随机选择 1997年 7月至 1999年 11月期间采用腹部横切口保留腹壁浅静脉施行子宫切除 10 0例作为观察组 ,选择同期纵切口子宫切除 10 0例作为对照组。两组年龄分布、子宫大小、手术指征等经χ2 检验 ,P>0 .0 5 ,其构成比无明显差异 ,具有可比性。1.2　麻醉 :两组病人均采用持续性硬膜外麻醉加腰麻。1.3　手术操作要点 :1耻骨联合上约 3 cm沿半月形腹壁横沟自右向左横行切开皮肤约 12～ …     

针刺治疗18例顽固性呃逆临床体会

呃逆,是临床常见症状。中医学认为,呃声高亢而短、响亦有力,多属实热;呃声低沉而长、气弱无力,多属虚寒。日常的打呃,呃声不高不低,无其它不适,多为食后偶然触犯风寒或因咽食急促所致,不属病态。若久病胃气衰败,出现呃逆,声低无力,则属虚证。临床上我们采用针刺疗法治疗呃逆18例,总有效率为94.4％。     

子宫动脉栓塞术治疗子宫切口假性动脉瘤2例

近年来由于剖宫产率持续增加,剖宫产所引起的并发症也日益增多,如子宫瘢痕处妊娠、憩室形成、子宫假性动脉瘤等引起的难治性晚期出血时常可见,但因诊断困难,常常误诊误治,危及生命。随着介入放射学技术的发展,对于那些难治性产后出血的患者,数字减影血管造影术(DSA)可提供更加准确的诊断,同时可进行子宫动脉栓塞来治疗上     

间变性大细胞淋巴瘤10例临床病理学观察

目的：探讨间变性大细胞淋巴瘤（anaplastic large cell lymphoma,ALCL）的病理形态、免疫表型特征及临床预后.方法：对10例ALCL进行形态学观察,免疫组化标记,并结合相关文献进行讨论.结果：本组ALCL男女之比为9∶1,平均年龄38.7岁.发生部位包括颈部淋巴结4例,小肠2例,头皮2例,纵膈 1例,支气管1例.ALCL形态表现多样,细胞呈多形性,大小不等,胞质丰富,细胞核大而不规则,呈扭曲、肾形、马蹄形及花环状,甚至见多核及瘤巨细胞.免疫表型：10例ALCL均阳性表达CD30,部分表达E-MA、CD3和CD45RO,不表达AE1/AE3、CD20、HMB45、S100、CD68和CD15.结论：ALCL是一种少见的非霍奇金淋巴瘤,诊断依赖于组织病理学及免疫组化标记,应与霍奇金淋巴瘤、弥漫性大B细胞淋巴瘤、低分化癌、恶性黑色素瘤等进行鉴别.     

出血性脑卒中病人住院初期家属需求及其影响因素分析

[目的]探讨出血性脑卒中病人入院72h内家属的需求及其影响因素。[方法]采用危重病人家属需求量表(CCFNI)和家属对照护及康复知识需求问卷调查入住神经外科重症监护室(NSICU)150例病人72h内家属的需求情况。[结果]出血性脑卒中病人入院早期家属的需求状况在病情保证需求、获取信息需求、亲近病人需求得分较高(P0.05);影响家属需求的因素有病人家属性别、年龄、文化程度、与病人的关系、医疗支付手段等11个因素。[结论]针对每位病人家属的不同需求,实施个体化护理,使其更好配合病人的治疗护理,促进病人康复。     

外科护理学课程设置与教学改革初探

外科护理学是护理专业的必修课程之一,也是护理专业核心课程。其内容主要涉及外科护理的基本理论、基本知识和基本技能,以及常见病、多发病病人的围手术期整体护理等,具有系统性突出、应用性强等特点。设置本课程目的在于全面掌握现代外科护理学的理论,形成科学的外科护理临床思路,提高临床观察、分析、判断和解决实际问题的能力,     

脐血S100B蛋白在早产儿脑损伤中的临床意义及预测价值

目的研究脐血S100B蛋白在早产儿脑损伤中的临床意义及预测价值。方法对59例孕周〈34周的早产儿用酶联免疫吸附试验检测脐血S100B蛋白;将母亲的胎盘胎膜组织行病理学检查,诊断有无绒毛膜羊膜炎;早产儿出生后72h内头颅超声检查,诊断有无脑损伤。结果早产儿脑损伤组脐血S100B蛋白【（3．40±1．38）μg,L】明显高于无脑损伤组【（1．37±1．19）μg／L】,两者差异有统计学意义（P＜0．05）。应用ROC工作曲线进行分析,以脐血S100B蛋白浓度3．18μg/L作为临界点,其诊断早产儿脑损伤的灵敏度为81．8％,特异度为89.2％。母亲有绒毛膜羊膜炎、出生体质量〈1500g及出生孕周〈32周的早产儿脑损伤发生率明显升高。结论脐血S100B蛋白检测是判断34周前早产儿有无宫内脑损伤的客观指标,并且对早产儿脑损伤具有一定的预测价值。     

Normal and degenerated rabbit nucleus pulposus cells in in vitro cultures: A biological comparison

This study examined the biological characteristics of normal and degenerated rabbit nucleus pulposus (NP) cells in vitro in order to provide seed cells for intervertebral disc (IVD) tissue engineering. A total of 8 adult New Zealand white rabbits underwent annulus puncture to establish models of intervertebral disc degeneration (IDD). Four weeks later, normal and degenerated NP cells were obtained. Cell morphology was observed by light and electron microscopy. Cell viability was measured by MTT assay. Cell cycle and expression of extracellular matrix (ECM)-related genes (aggrecan and type Ⅱ collagen) were determined by using flow cytometry and RT-PCR respectively. The growth curve of normal NP cells showed that the cells at passage 4 tended to slowly grow on the fifth day of culture. The density of normal NP cells at passages 5 to 7 was significantly less than that of the first-passage cells 2 or 3 days after seeding (P<0.05). The degenerated NP cells at passage 3 showed slow growth at 4th day. After 5 passages, the degenerated NP cells assumed stagnant growth and the growth seemed to stop at passage 7. The MTT assay revealed that for both normal and degenerated NP cells, the absorbance (A) value at passages 4-7 was obviously decreased as compared with that at passage 1 (P<0.05). Cell cycle analysis showed that the proportion of normal NP cells at G l phase was 65.4%±3.5%, significantly lower than that of degenerated NP cells at the same cell cycle phase with the value being 77.6%±4.8%. The degenerated NP cells were predominantly arrested at G 1 phase and failed to enter S phase. The expression of type Ⅱ collagen and aggrecan was significantly decreased with passaging. It was concluded that normal NP cells possessed good viability and proliferative capacity by the third passage, and they could secrete large amounts of ECM within this period. The normal NP cells may serve as seed cells for IVD tissue engineering.     

电灼治疗女性外阴鲍温样丘疹病12例疗效观察

鲍温拌丘疹病(Bowenoid papulosis．BP)常见于青年患生殖器、肛门部位的良性多发性丘疹损害,临床步见。女性外阴的BP病临床常被误诊为湿疣、炎症等;病理易诊断为原位癌,而当作恶性病变做过度的治疗。1994年3月～1998年3月治疗12例,疗效肯定．未见复发。现将结果报道如下。