Research hotspot and trend of microneedles in biomedical field: A bibliometric analysis from 2011 to 2020

BackgroundMicroneedles composed of arrays of micro-sized needles assembled on a patch, has attracted increasing interest in transdermal drug delivery due to its ease of use and lack of painful responses. Here, a bibliometric analysis was conducted to determine a hotspot and trend of microneedles in the biomedical field.MethodsAll relevant articles about microneedles between 2011 and 2020 were obtained from the databases of Web of Science (WOS) and PubMed of the National Center for Biotechnology Information (NCBI). A series of software such as VOSviwewer, the online bibliometric analysis website, CiteSpace, BICOMB and gCLUTO were used to process the data and get visual images. Processed data and visualized images were conducted to predict the trend of this research field.ResultsThe number of articles published over the last decade had increased rapidly (37 in 2011, 165 in 2020), the Journal of Controlled Release was the most productive journal in microneedle studies. The United States was the most productive country, while the Queens Univ Belfast topped the other institutions. Ryan F Donnelly was the most productive author in the field, while the two most cited articles were published by Gu Zhen group. More importantly, the research trend of microneedles had ranged from physicochemical properties and pharmacokinetics to insulin transdermal injection and vaccine development over the past decade. The four hot spots in microneedle studies, including skin rejuvenation, vaccines, fabrication technology and insulin delivery, were identified. Microneedle vaccination shows promising application prospects, and polymers are considered as the most promising materials for microneedles manufacturing.ConclusionsThis study will help researchers understand the hot spots and trends of microneedles in the biomedical field accurately and quickly. Moreover, the exploitation of novel polymeric microneedles will be a solid direction for subsequent research and development of transdermal drug delivery.     

新冠肺炎病症与中药材对应关系研究

利用文本挖掘技术和社会网络分析方法，基于中国知网（CNKI）数据库中新型冠状病毒肺炎相关文献数据构建分词词典、病症和药材词典，对文献的题名和摘要进行文本挖掘。将社会网络分析的对象拓展至病症和中药材，构建“病症-中药材”二模矩阵，对其进行共现可视化和量化分析，探索二者之间的对应关系。结果显示，部分病症和药材之间已经形成了较强且稳定的联系，与病症相比，药材之间的联系更为密切和深入。识别得到的病症和药材凝聚子群，能够为新型冠状病毒肺炎或其他肺炎的临床试验和相关研究提供参考。     

Identification of potential therapeutic target of naringenin in breast cancer stem cells inhibition by bioinformatics and in vitro studies

Cancer therapy is a strategic measure in inhibiting breast cancer stem cell (BCSC) pathways. Naringenin, a citrus flavonoid, was found to increase breast cancer cells’ sensitivity to chemotherapeutic agents. Bioinformatics study and 3D tumorsphere in vitro modeling in breast cancer (mammosphere) were used in this study, which aims to explore the potential therapeutic targets of naringenin (PTTNs) in inhibiting BCSCs. Bioinformatic analyses identified direct target proteins (DTPs), indirect target proteins (ITPs), naringenin-mediated proteins (NMPs), BCSC regulatory genes, and PTTNs. The PTTNs were further analyzed for gene ontology, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein–protein interaction (PPI) networks, and hub protein selection. Mammospheres were cultured in serum-free media. The effects of naringenin were measured by MTT-based cytotoxicity, mammosphere forming potential (MFP), colony formation, scratch wound-healing assay, and flow cytometry-based cell cycle analyses and apoptosis assays. Gene expression analysis was performed using real-time quantitative polymerase chain reaction (q-RT PCR). Bioinformatics analysis revealed p53 and estrogen receptor alpha (ERα) as PTTNs, and KEGG pathway enrichment analysis revealed that TGF-ß and Wnt/ß-catenin pathways are regulated by PTTNs. Naringenin demonstrated cytotoxicity and inhibited mammosphere and colony formation, migration, and epithelial to mesenchymal transition in the mammosphere. The mRNA of tumor suppressors P53 and ERα were downregulated in the mammosphere, but were significantly upregulated upon naringenin treatment. By modulating the P53 and ERα mRNA, naringenin has the potential of inhibiting BCSCs. Further studies on the molecular mechanism and formulation of naringenin in BCSCs would be beneficial for its development as a BCSC-targeting drug.     

国际冥想研究的现状及演进动态分析

目的：分析国际冥想研究的知识演化规律，为我国相关研究提供借鉴和参考。方法：基于Web of Science核心合集数据库中与冥想研究相关的7 271条文献记录，利用文献计量分析、聚类分析等方法对国际冥想研究现状和主题演进动态等进行探索分析。结果：近20年来冥想研究一直稳步发展，其实施方案、技术手段及涵盖人群等方面均有了不同程度的发展，我国与该领域处于领先地位的国家还存在一定差距。结论：冥想研究的发展前景较为明朗，未来正念冥想的实践与应用依然是冥想研究领域的热点话题，值得进一步研究和探讨。我国需增强与在该领域研究中领先的国家的交流和合作。     

计算机辅助医学图像三维重建的算法分析

背景：基于三维重建的医学图像可视化技术具有广阔的应用前景，是目前研究的热点内容。 目的：为医学图像三维重建时算法的选择提供帮助。 方法：分析面绘制重置方法和体绘制重置方法的实现原理、实现复杂度、实时显示情况等方面。 结果与结论：在对重建方法分析的基础上，总结出面绘制和体绘制两种方法各自的优势和弱点，分析在不同情况下如何进行算法的选择。     

PET脑功能图像感兴趣区提取和可视化研究

目的 提取和显示PET脑功能图像感兴趣区。方法 采用现在流行的SPM统计分析软件对PET脑功能图像进行图像预处理，提取感兴趣区域；采用三维面绘制技术显示并测量感兴趣区域。结果 在6例正常人针刺足三里PET脑功能图像感兴趣区提取、显示和测量中，得出针刺足三里穴位在大脑中央前后回和中央小叶前部产生功能增强，右侧颞叶前端、颞下回前端等区域也有增强。结论 实验结果与神经解剖学理论吻合。感兴趣区域的三维可视化有助于感兴趣区域的立体观察和计算。该方法有效、实用，为研究中医针刺足三里穴位与神经调节机制提供了新手段。     

超声监测儿童鼻骨骨折可视化复位的应用研究

目的探讨超声全程动态监测在儿童鼻骨骨折复位治疗中的应用价值。方法选取我院收治的鼻骨骨折患儿48例,术前均行超声及CT检查,术中超声全程动态监测复位器位置、骨折复位及鼻腔填塞情况,记录手法整复实施时间、二次手法整复率、CT扫描次数、CT评分、鼻通气评分等。结果48例患儿均在全身麻醉下行手法整复治疗,平均术前时间(22±6)h,手法整复实施时间(22±6)h,均未进行二次手法调整,CT扫描次数平均2次/人,平均住院时间(4.5±1.6)d。术前CT评分2分以下者48例,术后CT评分2分以上者47例;鼻通气评分术前5分以上者9例,术后5分以上者47例,差异均有统计学意义(均P<0.05)。结论超声全程动态监测下行儿童鼻骨骨折闭合复位,可快速、准确地协助临床更安全地完成诊治工作。     

基于共词分析的循证心血管可视化研究

目的通过关键词共现分析和作者共现分析的方式,研究国内外循证心血管领域的研究热点、学科分支和发展趋势。方法通过检索中国生物医学数据库和Medline数据库,分别收集相关研究文献,并提取关键词词频、作者发文数和核心研究单位,对核心关键词和核心发文作者建立共现矩阵,使用Ucinet绘制可视化网络图。结果成功得到核心关键词和核心作者可视化网络。中文可视化网络包括循证医学、治疗和护理3个方面,英文可视化网络包括evidence-based medicine、therapy和treatment outcome 3个方面。而在作者合作可视化网络中,国内作者合作关系较为松散,合作团体较小,国外作者合作关系较为紧密,合作团体较大。国内核心研究机构分布于循证研究重点高校,国外则以发达国家为主。结论国内循证心血管研究领域总体发展趋势较好,但较国外相比仍存在一些不足,需要进一步加强重点研究,调整研究规划。