红外线照射和碘伏浸浴治疗糖尿病患者肢端感染的护理

目的探讨红外线照射和碘伏浸浴对糖尿病患者肢端感染的治疗效果。方法将105例患者随机分为治疗组60例和对照组45例。治疗组采用红外线照射和碘伏浸浴治疗,对照组采用常规换药治疗。并观察1~4个月治疗效果。结果治疗组有效率达98.33%,对照组有效率达26.66%,两组比较有显著性差异(P<0.01)。结论采用红外线照射和碘伏浸浴治疗糖尿病患者肢端感染,操作方法简单、费用低、缩短了疗程、提高了治愈率,可作为糖尿病慢性创面的常规治疗方法之一。     

IL-1α and ATP mRNA expression after ultraviolet irradiation in human keratinocyte original-SCC 12F cells

Background Generally speaking, undifferentiated keratinocytes may synthesize a larger amount of IL-α and its production is decreased as cells complete differentiation gradually. Ultraviolet B (UVB)light can signigicantly stimulate the production and release of some cytokines. In this study we investigated the influence of UVB irradiation on IL-1α and adenosine triphosphoric (ATP) mRNA expressions in the human keratinocyte (KC) of original squamous cell carcinoma line (SCC 12F cells).Methods The cultured SCC 12F cells were irradiated with 30 mJ/cm^2 of UVB. Northern blot was employed to analyze the expression of IL-1α and ATP mRNA.Results There was a constitutive expression of IL-1α mRNA in SCC 12F cells. The expression increased in culturing time in regular KC medium and reached the highest expression at 120 hours.The expression level of IL-1α was up-regulated with two peaks at 6 hours and 72 hours, respectively after UVB irradiation. In comparison with IL-1α mRNA expression, ATP mRNA was down-regulated,with similar biphasic peaks, compared with the sham irradiated group.Conclusions SCC12F cells may express IL-1α mRNA constitutively. After UVB irradiation, the mRNA expression of IL-1α and ATP will show opposite effect because of inflammation/immunity and energy consumption mechanisms.     

放射线敏感性自杀基因体外杀伤肝癌细胞的实验研究

目的观察可被放射线转录激活的早期生长反应基因 1(earlygrowthresponse 1,Egr 1)启动子驱动的单纯疱疹病毒胸苷激酶基因 (herpssimplexvirusthymidinekinase ,tk)对肝癌细胞的高效杀伤作用。方法构建以Egr 1为启动子 ,以tk为目的基因的重组质粒pET ,经脂质体介导转染人肝癌细胞株SMMC 772 1,命名为SMMC/ET细胞 ,经G418抗性筛选、60 Co γ射线照射后 ,加入前药丙氧鸟苷 (ganciclovirGCV) ,测定其对肝癌细胞的杀伤作用。结果与对照组肝癌细胞 ( 0 88± 0 12 )相比 ,经γ射线照射后 ,前药GCV可明显提高对SMMC/ET肝癌细胞的杀伤效率 ( 0 0 7± 0 0 3) (P <0 0 0 1)。结论放射敏感性自杀基因在γ射线作用下可以显著提高对肝癌细胞的杀伤作用。     

60Coγ射线诱导的K562细胞增殖抑制和凋亡的研究

目的：观察不同剂量的γ射线和照后不同时间的K562细胞的增殖抑制及凋亡情况。方法：细胞动态计数；^3H-TdR掺入分析；利用ApoAlert Mitochondrial Membrane Sensor Kit在荧光显微镜下观察荧光情况和在流式细胞仪上测定凋亡细胞的比例。结果：在不同剂量照射和照后的不同时间处，^60Coγ射线诱导的细胞增殖抑制和凋亡情况明显不同：细胞增殖抑制情况与受照剂量的大小呈正相关，细胞凋亡比例与受照剂量的大小未见明显相关性，而表现为在照后6h处凋亡细胞较多。结论：^60Coγ射线照射引起的肿瘤细胞增殖抑制有一定的发生时机，并且表现出剂量相关性，而其引起的凋亡效应则更多地表现为时间相关性。     

强直性脊柱炎在骶髂关节的CT表现(附25例报告)

目的探讨强直性脊柱炎在骶髂关节的CT表现. 资料与方法对25例临床、实验室检查已确诊的强直性脊柱炎患者的骶髂关节行CT扫描,分析其征象. 结果强直性脊柱炎患者骶髂关节改变的基本征象(1)局限性骨质稀疏、侵蚀、破坏;(2)骨质增生硬化;(3)关节间隙增宽或变窄;(4)关节强直;(5)关节软组织肿胀. 结论骶髂关节CT检查有助于提高强直性脊柱炎的早期诊断率.     

B超、X线在输尿管疾病诊断中的应用比较

目的 :评价B超和X线对输尿管疾病的诊断价值。方法 :对 6 3例输尿管疾病的B超、X线检查结果进行对比分析。结果 :B超、X线对输尿管疾病均有较高的诊断率 ,分别为 81.0 %和 90 .5 % ,B超对位于上段的输尿管疾病诊断率较高 ,X线则可补充其不足。结论 :B超应为首选方法 ,二者酌情联合 ,可提高输尿管疾病的诊断率     

The behavior of single crystals of NaCl: Ca, Mn exposed to gamma rays

The behavior of single crystals of NaCl: Ca²⁺, Mn²⁺ exposed to gamma rays was explored for its potential usage as a dosimeter. The study was focused to the effect of dose and dose rate. The crystals were analyzed using thermoluminescence (TL). The productions of irradiation defects in the solid were correlated with the glow curve. F-centers were measured as function of the dose. The bleaching of the F-centers produced a decrease of the peak of the glow curve. The results showed that the response is linear in an interval up to 60 Gy.     

人工北冬虫夏草的近红外特性研究

北冬虫夏草是稀有中药之一,组织培养提供了药材的新来源。本文比较了冬虫夏草、人工北虫草及其培养基等六种样品的近红外光谱的特性。认为人工北虫草与野生虫草的光谱特性相似;污染杂菌的样品表见为不同的光谱特征;所以,近红外分析可以作为成分鉴别的快速方法之一。