中医骨折三期治疗对家兔骨折骨膜组织中VEGF及VEGF mRNA表达的影响

目的:研究中医骨折三期治则对家兔桡骨骨折愈合过程中内外骨膜及骨髓组织VEGF及VEGFmRNA表达的影响,探讨中药治疗骨折的分期治疗的合理性。方法:140只雄性新西兰白兔,无菌状态下手术,在家兔双侧桡骨下1/3段,造成3mm完全骨质缺损的骨折愈合模型。采用计算机产生随机数字的方法,将家兔随机均分为4组:中药三期治疗组、中药一期治疗组、阳性药物对照组以及模型对照组,分别给予三期治疗、一期治疗、阳性药物治疗以及等量蒸馏水。于术后3、6、9、14、28、42、56d每组随机处死5只家兔,取左侧桡骨标本作为观察对象,采用免疫组化和原位杂交方法检查外骨膜、内骨膜和骨髓组织中VEGF及VEGFmRNA表达,通过图像分析测量各种组织中VEGF及VEGFmRNA表达强度。结果:各中药治疗组对于术后骨折端外骨膜、内骨膜和骨髓组织VEGF和VEGFmRNA表达均有促进作用,三期治疗组有高于其他三组的趋势(P0.05)。结论:中药分期治疗对骨折愈合过程中外骨膜、内骨膜和骨髓组织VEGF及VEGFmRNA表达的促进作用更加明显,骨折分期治疗是必要的。     

股骨头坏死误诊现状及分析

探讨股骨头坏死误诊的主要原因,有利于减少其误诊及漏诊的发生率,提高股骨头坏死的诊断水平。本文分析近15年国内有关股骨头坏死误诊文献。对易与股骨头坏死混淆的9种疾病进行鉴别分析。股骨头坏死早期易误诊为其它疾病;而股骨头坏死中晚期的一些症状、体征及影像表现与一些疾病相似,易将这些疾病误诊为股骨头坏死。髋关节疾病病理改变的不同始发部位,在相应的影像异常表现有各自不同的特异性表现。     

高血压不同并发症患者CRP、IL-6水平变化及血脂康干预的疗效观察

目的:观察原发性高血压(EH)不同合并症患者体内炎症反应的程度与中药血脂康的干预疗效。方法:观察EH患者120例,根据其合并症的不同,分为单纯EH组30例,EH合并心房颤动(EH-AF)组30例,EH合并腔隙性脑梗死(EH-LI)组30例,EH合并左心室肥厚(EH-LVH)组30例,同时选择30例健康体检者作为对照组。每组EH患者随机又分为两个亚组,即常规治疗亚组(主要运用钙拮抗剂ACEI、ARB、利尿剂、β受体阻断剂等药物治疗8周)和血脂康干预亚组(常规治疗加血脂康1.2g/d)。药物治疗前后检测血hs-CRP、IL-6水平。结果:(1)所有入选EH患者的hs-CRP、IL-6水平显著高于正常对照组(P<0.05);(2)EH-AF组、EH-LI组、EH-LVH组的hs-CRP、IL-6水平无明显差异(P>0.05),但均显著高于单纯EH组(P<0.05);(3)血脂康组和常规治疗组治疗后,其hs-CRP、IL-6水平均显著下降(P<0.05),两组间比较,血脂康组明显更优(P<0.05);(4)血脂康组和常规治疗组治疗后,其血压均显著下降(P<0.05),两组间比较,血脂康组的血压下降幅度更加显著(P<0.05)。结论:炎症反应指标hs-CRP、IL-6的上升与EH并发症的产生关系密切,血脂康能够显著下调EH患者的hs-CRP、IL-6和血压水平,提示联合应用降压药物和血脂康对于EH患者可能更为有益。     

穴位刺激防治含顺铂方案化疗患者消化道副反应的临床研究进展

近年来恶性肿瘤发病率逐年升高,抗肿瘤药物不断更新,化疗在肿瘤治疗过程的各个阶段不可或缺。然而,化疗药物所致的消化道副反应却是临床治疗十分棘手的难题之一,尤其在含有顺铂的联合化疗方案中更常见,严重的恶心呕吐影响化疗的顺利进行。因此改善化疗药物所致的消化道副反应,不仅可以有效改善肿瘤患者的生活质量,而且对于提高肿瘤患者的化疗耐受性具有重大的意义。早期中医治疗化疗药消化道副反应临床研究多为针刺单穴或电针,近年来该领域取得了一定的成就,因此本研究分别从穴位注射、穴位按压、单纯针刺、灸法、电针等方面将近10年来相关临床研究概述如下。     

关节镜下内侧半月板囊肿13例治疗体会

目的 探讨关节镜治疗内侧半月板囊肿的临床疗效.方法 2011年6月至2013年1月,我科采用关节镜治疗内侧半月板囊肿13例,术后随访3个月,观察治疗前后Lysholm膝关节功能评分变化及临床疗效.结果 术前Lysholm评分为49～77分,平均68.2±2.4分,术后为86～95分,平均91.1±1.7分,术后评分明显高于术前(P＜0.05);根据Sarimo标准,本组中优10例,良3例,优良率100％.结论 采用关节镜治疗内侧半月板囊肿,取代了传统的大切口囊肿及内侧半月板全切,最大程度地保留了正常半月板,并可同时行半月板损伤修复,术后疗效好,膝关节功能恢复好,值得临床推广应用.     

关节镜下微骨折技术治疗距骨骨软骨损伤60例疗效观察

目的探讨关节镜下微骨折技术治疗距骨骨软骨损伤的疗效。方法采用关节镜下微骨折技术治疗距骨骨软骨损伤60例,观察踝关节功能评分及疼痛评分变化。结果 60例患者术后踝关节功能评分较术前平均提高20.1分,差异具有统计学意义(P<0.05);术后疼痛评分较术前平均降低5.1分,差异具有统计学意义(P<0.05)。结论关节镜下微骨折技术治疗距骨骨软骨损伤,具有显著的临床疗效,值得推广应用。     

中医综合疗法治疗神经根型颈椎病后肩臂疼痛症状复发率的临床研究

目的观察经中医综合疗法治疗神经根型颈椎病康复后肩臂疼痛症状复发率的临床疗效。方法将480例神经根型颈椎病患者随机分为治疗组和观察组各240例,治疗组给予脊柱旋捉手法治疗、口服中药、颈椎康复操的中医综合疗法治疗,对照组给予颈椎牵引治疗、口服西药、佩戴颈围的常规治疗,比较治疗后疗效及1个月后随访临床控制、显效患者肩臂疼痛症状复发率情况。结果治疗组控显率、有效率均高于对照组,差异有统计学意义（P〈0．05）;治疗组复发率低于对照组,差异亦有统计学意义（P〈0．05）。结论中医综合疗法可有效治疗神经根型颈椎病且有复发率低的优势。     

Protein losing enteropathy caused by eosinophilic gastroenteritis: A case report

Protein losing enteropathy (PLE), a very rare disease with hypoproteinemia and edema as its characteristics, is caused by various diseases resulting in protein depletion from the gut. The diagnosis is relatively difficult due to its complex pathogeneses. The present paper reported a case whose symptom started with acute diarrhea and hypoproteinemia. Gastrointestinal endoscopies showed digestive ulcers and colon polyp. The treatments contained albumin infusion, Chinese herbal decoction and other symptomatic therapies. The hypoproteinemia become even worse and edema occurred after 4 days' treatment. A larger dose of albumin infusion (40-60 g/d) and modified herbal decoctions were prescribed. A final diagnosis of eosinophilic gastroenteritis (EG) complicated with PLE was confirmed by histopathological examination of a repeated gastroscopy. After three weeks' treatment, the serum albumin level was raised and the edema subsided gradually. In conclusion, herbs may have an effect on PLE patients, but PLE resulting from EG is very complex and easy to misdiagnose, especially in atypical conditions. Further studies are required to find the exact mechanisms.     

化疗相关心脏毒性问题研究进展

化疗作为肿瘤治疗手段的基石之一,可引起心力衰竭、冠脉病变、瓣膜病、心律失常等心脏毒性事件,其相关近期、远期心脏毒性问题均需受到临床医生重视。近年随着肿瘤心脏病学交叉学科的发展,化疗药物相关心肌损伤问题在病理生理机制、诊断、治疗等方面均取得了一定进展,但仍然存在诸多问题。该综述从不同化疗药物引起心血管事件的流行病学、临床特点、机制、诊断及治疗策略方面简要介绍其心脏毒性的诊治问题,为临床实践提供参考。     

Huogu I formula prevents steroid-induced osteonecrosis in rats by down-regulating PPARγ expression and activating Wnt/LRP5/β-catenin signaling

Objective

To investigate the effects of Huogu I formula on regulation of lipid metabolism in steroid-induced osteonecrosis of the femoral head (SONFH) rats and verify our hypothesis that Huogu I formula regulates lipid metabolism by down-regulating peroxisome proliferator-activated receptor gamma (PPARγ) expression and activating Wnt signaling pathways.

Methods

Eighty-five rats were divided into four groups: control, model, Huogu 15 g/kg and Huogu 30 g/kg. Six weeks later, animals were anaesthetized, femora were dissected for histopathological examination of the osteonecrotic changes and repair processes, micro computed tomography (Micro-CT)-based micro-angiography was performed to assess vascularization. Serum lipid levels were detected by haematological examination. The expressions of PPARγ, Wnt3a, low density lipoprotein receptor-related protein 5 (LRP5) and β-catenin were evaluated by immunohistochemistry, Western blot and quantitative real-time polymerase chain reaction analyses.

Results

The incidence of osteonecrosis, ratio of empty lacuna, adipose tissue area and adipocyte perimeter in the bone marrow were dramatically lower in the Huogu I formula treatment groups. By micro-CT quantification, Huogu I formula treatment dose-dependently increased vessel volume, vessel surface, percentage of vessel volume and vessel thickness of the femoral heads of SONFH rats. Levels of serum lipid in Huogu 15 g/kg and Huogu 30 g/kg groups reduced significantly. Huogu I formula treatment could suppress the expression of PPARγ and increase the expressions of Wnt3a, LRP5 and β-catenin at both protein and mRNA levels.

Conclusion

The results of our present study highlight the lipid-lowering potential of Huogu I formula, and provide further evidence of the involvement of the PPARγ inhibition and Wnt/LRP5/β-catenin signaling activation in the effects of Huogu I formula.