Effect of enhancer of zeste homolog 2 inhibitor GSK126 on the proliferation and apoptosis of tongue squamous cell carcinoma

目的 观察zeste基因增强子同源物2（EZH2）抑制剂GSK126对人舌鳞状细胞癌细胞体外增殖与凋亡的影响,并探讨其相关机制,为舌鳞状细胞癌的临床治疗提供新思路。方法 将不同浓度的GSK126作用于舌鳞状细胞癌CAL-27细胞,通过甲基噻唑基四唑（MTT）、克隆形成、5-乙炔基-2’脱氧尿嘧啶核苷（EdU）荧光染色实验检测药物对细胞增殖能力的影响;采用Hoechst33342荧光染色、JC-1法观察细胞凋亡情况;采用Western blot法检测CAL-27细胞内相关蛋白细胞外调节蛋白激酶（ERK）、磷酸化的细胞外调节蛋白激酶（p-ERK）、Bax、Bcl-2、Cleaved caspase-9的表达水平。结果 GSK126能抑制CAL-27细胞增殖并对细胞凋亡有促进作用。GSK126能下调细胞内p-ERK、Bcl-2的表达水平,同时可增加Bax、Cleaved caspase-9的表达（P<0.05）。结论 GSK126可抑制舌鳞状细胞癌CAL-27细胞的增殖,并且能促进其凋亡,其机制可能与抑制MEK/ERK信号通路以及激活Bax/Bcl-2通路有关。     

不同制备形态对短冠磨牙髓腔固位冠抗折性影响的研究

目的:比较3种不同预备深度和有无牙本质肩领对髓腔固位冠修复体及牙体抗折性的影响,为临床上髓腔固位冠修复短冠磨牙的牙体预备提供参考。方法:收集牙颈部至根尖形态完整的上颌离体恒磨牙30颗。设置3种髓腔预备深度3 mm、4 mm、5 mm,两种边缘预备形式,随机分为6组,通过CAD-CAM技术制作氧化锆髓腔固位冠进行修复,随后进行抗折强度测试,记录折裂负荷值以及折裂模式。结果:6组样本的折裂负荷值不管是在对接组还是肩台组中,最大载荷值均为3 mm组<4 mm组<5 mm组,但组间两两比较无显著性差异。肩台组的最大载荷值大于对接组,在3 mm和4 mm组中差异性有统计学意义。6组样本折裂模式中均以不可修复折裂为主,5 mm组中均呈现不可修复性折裂。结论:通CAD-CAM技术制作的氧化锆髓腔固位冠修复上颌短冠磨牙时,3 mm和4 mm的髓腔预备深度均能满足临床抗折强度要求,但不宜超过4 mm;另外,预备形态中引入1 mm的牙本质肩领可作为临床一种有效增加牙体和修复体抗折强度的预备方式。     

某部女新兵集训期性激素变化、月经紊乱及相关研究

目的研究某部女新兵集训引起性激素、体质量及心理变化等对月经的影响。方法选取45名女新兵分别在集训前、集训第3个月时检测体内性激素;对女新兵用问卷调查、症状自评量表测评及磅秤测量体质量等方法调查分别在集训前、中、后半年及1年月经、心理及体质量等变化。结果集训中,女兵黄体期及卵泡期FSH、HL、P显著降低,E2、T、PRL显著升高;月经期E2、P升高显著,FSH、HL、T及PRL无显著性差异;月经紊乱及月经疾患发生率显著增高;体质量减少者显著增多。结论集训中女新兵性激素、体质量及心理变化对月经影响明显,故重视集训中女兵生殖卫生保健十分必要。     

Puerarin attenuates cognitive dysfunction and oxidative stress in vascular dementia rats induced by chronic ischemia

Objective: To explored the effects of puerarin on cognitive deficits and tissue oxidative stress and the underlying mechanisms. Methods: 6 to 8 week old male Wistar rats were adopted as experimental animals. Morris water maze (MWM) test was adopted to test the learning and memory function of rats. MDA, glutathione peroxidase and total thiol assessment was done to reflect the oxidative stress in the brain tissue. Cell Counting Kit-8 (CCK8) and flow cytometry (FCM) were performed to examine the cell viability and apoptosis rate. Reactive oxygen species (ROS) generation was determined by the 2’, 7’-dichlorofluorescein diacetate (DCFH-DA) assay. qPCR and Western blot (WB) were adopted to test the molecular function mechanisms of puerarin. Results: Our results indicated a protective effect of puerarin on vascular dementia. Administration of puerarin could improve the impaired learning and memory function. The levels of MDA were partially decreased by puerarin. The levels of glutathione peroxidase and total thiol were partially restored. Cell viability was improved in a dose-dependent pattern (P < 0.05). Cell apoptosis rate was reduced in a dose-dependent pattern (P < 0.05). Puerarin could scavenge ROS generation induced by pre-treatment of hydrogen peroxide. The results showed up-regulated levels of Nrf2, FoxO1, FoxO3 and FoxO4 (P < 0.05). Conclusion: Puerarin is protective on the vascular dementia by reducing oxidative stress and improving learning and memory functions. On the molecular level, Nrf2, FoxO1, FoxO3 and FoxO4 were up regulated by puerarin.     

奥拉西坦对心肌缺血/再灌注损伤的保护作用

目的 研究奥拉西坦对心肌缺血/再灌注损伤(MI/RI)的保护作用及其机制。方法 32只大鼠依据随机数表法分为4组,每组8只:(1)假手术组;(2)MI/RI组;(3)低剂量组(奥拉西坦30mg/kg);(4)高剂量组(奥拉西坦50mg/kg)。低剂量组和高剂量组的16只大鼠手术前除正常饲养外,通过灌胃方式给予对应剂量的奥拉西坦,1次/d,连续7d。结扎大鼠冠状动脉前降支,建立MI/RI模型。酶联免疫吸附测定大鼠血清中肌酸激酶同工酶MB(CK-MB)、乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、白细胞介素-6(IL-6)和丙二醛(MDA)。测定心肌梗死面积百分比、心肌细胞凋亡率和Bcl-2/Bax比值。采用SPSS 21.0软件进行统计分析。组间比较采用单因素方差分析。结果 与MI/RI组比较,低剂量组和高剂量组大鼠的心肌梗死面积百分比(36.2%和13.6%和22.3%)、心肌细胞凋亡率(45.4%和30.6%和21.3%)、血清中CK-MB[(97.50±8.54)和(75.24±5.62)和(63.10±7.23)U/L]、LDH[(900.02±57.74)和(660.62±49.84)和(577.37±54.09)U/L]、MDA[(88.84±3.26)和(66.47±6.25)和(57.08±4.99)U/L]和IL-6[(186.39±10.18)和(153.34±6.81)和(143.31±9.08)pg/ml]含量均显著降低(P<0.05),而SOD[(15.22±1.24)和(24.80±3.32)和(34.82±3.66)U/ml]和Bcl-2/Bax比值(0.19和0.47和1.16)显著增加(P<0.05)。结论 奥拉西坦可能通过减轻氧化应激反应来抑制线粒体途径的细胞凋亡来保护MI/RI的心肌。     

小鼠骨髓间充质干细胞向胰岛素分泌细胞诱导分化过程中调控PDX-1基因表达miRNAs的鉴定

目的实现小鼠骨髓间充质干细胞(BMSCs)向胰岛素分泌细胞(IPCs)的诱导分化并对分化过程中可能调控胰十二指肠同源异型盒基因-1(PDX-1)基因表达miRNAs进行鉴定。方法首先分离培养BMSCs,应用conophylline和尼克酰胺将其诱导分化为IPCs,采用双硫腙(DTZ)染色和免疫荧光检测胰岛素的表达。然后采用靶基因预测软件miRanda和Target Scan对调控PDX-1基因表达miRNAs进行预测并通过双荧光素酶报告基因系统鉴定。实时定量聚合酶链反应(Real-time PCR)检测诱导分化过程中miRNAs及PDX-1的表达。结果诱导分化后的细胞双硫腙染色呈猩红色,免疫荧光化学显示有胰岛素表达。生物信息学方法预测得到4个可能调控PDX-1表达的miRNAs,通过双荧光素酶报告基因系统检测发现其中的miR-149和miR-346能结合到PDX-1 mRNA的3’UTR并有效抑制其表达。Real-time PCR检测结果表明,miR-149和miR-346的表达水平与PDX-1表达呈负相关。结论 miR-149和miR-346能负性调控IPCs诱导分化过程中PDX-1的表达。     

Interleukin-18 promoter polymorphisms and plasma levels are associated with increased risk of periodontitis: a meta-analysis

Objective

Emerging evidence has showed that interleukin-18 (IL-8) promoter polymorphisms and plasma IL-18 levels may be associated with increased risk of periodontitis, but individually published results are inconclusive. The aim of this meta-analysis was to derive a more precise estimation of these associations.

Methods

A literature search of PubMed, Cochrane Library, Embase, Web of Science, SpringerLink, China BioMedicine and China National Knowledge Infrastructure databases was conducted on articles published before April 1st, 2013. Crude odds ratio (OR) or standardized mean difference (SMD) with 95 % confidence intervals (CI) were calculated.

Results

Nine case–control studies were included with a total of 576 periodontitis patients and 458 healthy controls. Two common polymorphisms (?607A > C and ?137G>C) in the IL-18 gene were addressed. Our meta-analysis results indicated that the C variant of IL-18 ?607A>C polymorphism was associated with increased periodontitis risk (C allele vs. A allele: OR = 1.86, 95 % CI: 1.30–2.65, P = 0.001; AC+CC vs. AA: OR = 2.64, 95 % CI: 1.34–5.21, P = 0.005). There was also a significant association between the C variant of IL-18 ?137G>C polymorphism and an increased periodontitis risk (C allele vs. G allele: OR = 1.47, 95 % CI: 1.13–1.91, P = 0.004; GC+CC vs. GG: OR = 1.66, 95 % CI: 1.21–2.29, P = 0.002). Furthermore, the mean levels of plasma IL-18 of periodontitis patients were also higher than those of healthy controls (SMD = 1.18, 95 % CI: 0.51–1.85, P = 0.001).

Conclusion

The current meta-analysis suggests that IL-18 promoter polymorphisms and plasma IL-18 levels are associated with increased risk of periodontitis. IL-18 promoter polymorphisms and elevated plasma IL-18 levels may be useful biomarkers for predicting the development of periodontitis.     

冠心病患者经皮冠状动脉介入术后左心房压力、功能变化及其与左心室功能的关系

目的应用组织多普勒成像(TDI)及二维斑点追踪显像(2D-STI)技术评价冠心病(CHD)患者经皮冠状动脉介入术(PCI)前后左心房压力和功能变化及其与左心室功能关系,为临床CHD的治疗提供更有效信息。方法对30例PCI术治疗的CHD患者分别在PCI术前、术后1周及术后3月经超声心动图测量并计算左心房最大容积(LAV_(max))、最小容积(LAV_(min))、左心房收缩前容积(LAV_(pre))、左心房被动排空分数(LAPEF)、左心房主动排空分数(LAAEF)、左心室射血分数(LVEF)、二尖瓣舒张早期速度峰值(E)及晚期速度峰值(A);采用TDI测量并计算二尖瓣环舒张早期速度(e’)、左心房平均压力(mLAP)、E/A及E/e’;采用2D-STI测量并计算左心室整体纵向应变(GLS),左心室收缩期、舒张早期及晚期左心房各壁平均峰值应变率(mSRs,mSRe,mSRa)。另选30例冠状动脉造影检查冠状动脉狭窄率30%的病例作为对照组。结果 CHD组与对照组比较,各时间段LVEF、LAPEF、E、E/A、e’、mSRs、mSRe、GLS均下降,A、E/e’、mLAP、LAV_(min)、LAV_(max)、LAV_(pre)、LAAEF、mSRa均升高(P0.05)。CHD组PCI术后1周与术前比较,各项指标差异均无统计学意义(P0.05);CHD组PCI术后3月与术前、术后1周比较,E、e’、E/A、mSRs、mSRe、GLS、LVEF、LAPEF均上升,A、E/e’、mLAP、LAV_(min)、LAV_(max)、LAV_(pre)、LAAEF、mSRa均下降(P0.05)。CHD组PCI术后3月mLAP与GLS、mSRe、LAV_(min)、LAV_(max)、LAV_(pre)呈正相关(r=0.548,0.871,0.851,0.858,0.857,P0.01),与LVEF、E/A、mSRa、mSRs、LAPEF呈负相关(r=-0.800,-0.884,-0.898,-0.829,-0.427,P0.05)。结论 CHD患者左心房及左心室功能在PCI术后3月较术后1周、术前明显改善;左心房压力与功能变化可以综合反映CHD患者PCI术后左心房及左心室功能改变,二者功能紧密联系。     

氯沙坦对大鼠脑缺血再灌注损伤的保护作用及分子机制

目的研究氯沙坦对大鼠脑缺血再灌注(IR)损伤的保护作用及分子机制。方法 80只成年雄性SD大鼠随机分为假手术组、IR组、2. 5 mg/kg氯沙坦组、5. 0 mg/kg氯沙坦组、5. 0 mg/kg氯沙坦+LY组。2. 5 mg/kg氯沙坦组、5. 0 mg/kg氯沙坦组、5. 0 mg/kg氯沙坦+LY组在造模前连续给予氯沙坦灌胃干预14天,5. 0 mg/kg氯沙坦+LY组在造模前30 min给予磷脂酰肌醇3激酶(PI3K)抑制剂LY294002侧脑室注射。采用线栓法建立大鼠脑IR损伤模型,在再灌注后24 h评价神经功能缺损并测定脑组织含水量、细胞凋亡情况、凋亡相关基因表达水平。结果与假手术组比较,IR组大鼠的脑组织含水量、神经功能缺损评分、TUNEL阳性率及脑组织中Bcl-2相关X蛋白(Bax)/GAPDH、细胞色素C(Cyt C)/GAPDH、Cleaved-caspase-3/Pro-caspase-3的水平均显著增加,脑组织中B淋巴细胞瘤2蛋白(Bcl-2)/GAPDH、p-PI3K/PI3K、p-AKT/AKT的水平显著减少;与IR组比较,2. 5 mg/kg氯沙坦组和5. 0 mg/kg氯沙坦组大鼠的脑组织含水量、神经功能缺损评分、TUNEL阳性率及脑组织中Bax/GAPDH、Cyt C/GAPDH、Cleaved-caspase-3/Pro-caspase-3的水平均显著减少,脑组织中Bcl-2/GAPDH、p-PI3K/PI3K、p-AKT/AKT的水平显著增加;与5. 0 mg/kg氯沙坦组比较,5. 0 mg/kg氯沙坦+LY组大鼠的脑组织含水量、神经功能缺损评分、TUNEL阳性率及脑组织中Bax/GAPDH、Cyt C/GAPDH、Cleaved-caspase-3/Pro-caspase-3的水平均显著增加,脑组织中Bcl-2/GAPDH、p-PI3K/PI3K、p-AKT/AKT的水平显著减少。结论氯沙坦通过上调PI3K/AKT通路减轻大鼠脑IR损伤。     

两种方法治疗垂直不稳定型股骨颈骨折的近期疗效对比

目的评价单纯空心螺钉与3枚空心钉加内侧支撑钢板两种方式处理股骨颈Pauwels Ⅲ型骨折的近期治疗效果。方法选取锦州地区55例股骨颈Pauwels Ⅲ型骨折患者,随机分为空心加压螺钉组30例（A组）,3枚空心螺钉+支撑钢板组25例（B组）。记录术后髋关节Harris评分,对比各项手术指标、愈合时间、复位质量、股骨头坏死与骨不连等并发症的发生情况。结果A组手术时间（68.73±4.91）min,低于B组（110.76±5.85）min;A组术中出血量（69.47±5.15）mL,低于B组（110.92±10.10）mL;A组的骨折愈合时间（5.03±0.47）个月,高于B组（4.37±0.56）个月;差异均有统计学意义（P<0.05）。两组内固定失效和复位情况的对比,B组优于A组（P<0.05）;在股骨头坏死和骨折不愈合方面的比较,A组高于B组（P>0.05）。术后第1、3个月的Harris分值,A组为（70.67±1.88）分、（74.83±3.69）分,低于B组（71.60±1.78）分、（76.60±3.10）分,差异无统计学意义（P>0.05）;术后第6、12个月的Harris分值,A组为（78.97±1.88）分、（88.17±3.36）分,低于B组（80.08±1.93）分、（92.04±3.72）分,差异有统计学意义（P<0.05）。结论采用空心钉+支撑钢板治疗骨折线较垂直的股骨颈Pauwels Ⅲ型骨折的效果优于单纯3枚空心钉,拥有骨折复位质量好、更加牢靠的固定、有效抵抗垂直剪切力、缩短愈合时间,患髋功能恢复好等优点。