化湿利水泄浊法对自发性高血压大鼠主动脉血管结构的影响

为观察化湿利水泄浊法(DTM)对自发性高血压大鼠(SHR)主动脉血管结构的影响。27只12周龄SHR，随机分为模型组(B)、化湿利水泄浊组(C)、依那普利组(D)，另选同周龄大鼠9只作为正常对照组(A)，灌胃给药8周。测量清醒状态下尾动脉收缩压，及血浆内皮素(ET)、降钙基因相关肽(CGRP)。血管壁厚度／管外径(WT／LD)、管壁面积／管面积(MA／WA）、管腔面积／管面积(LA／WA）。主动脉血管平滑肌细胞增殖核抗原(PCNA)的表达。结果：与A组比较，收缩压B组明显升高，C组明显下降；B组ET浓度升高，C组下降；B组主动脉超微结构见中膜纤维化改变，细胞间隙增宽，平滑肌细胞排列紊乱等改变；C、D组动脉超微结构改变较轻；B组平滑肌细胞PCNA表达明显增强，C、D组降低。提示化湿利水泄浊法能部分逆转SHR主动脉的超微结构改变，抑制平滑肌细胞增殖可能是其发挥作用的机制之一。     

Can dogs serve as stress mediators to decrease salivary cortisol levels in a population of liberal arts college undergraduate students?

The steroid hormone cortisol can be used to measure physiological stress in humans. The hypothalamic-pituitary-adrenal (HPA) axis synthesizes cortisol, and a negative feedback cycle regulates cortisol depending on an individual's stress level and/or circadian rhythm. Chronic stress of college undergraduate students is associated with various adverse health effects, including anxiety and depression. Reports suggest that stress levels have risen dramatically in recent years, particularly among university students dealing with intense academic loads in addition to COVID-19 pandemic-related uncertainty. The increasing rate of mental illness on college campuses necessitates the study of mediators potentially capable of lowering stress, and thus cortisol levels. Research on mediation techniques and coping mechanisms have gained traction to address the concerning levels of stress, including the employment of human-animal interaction sessions on college campuses. In this study, human-canine interaction as a stress mediation strategy for undergraduate students was investigated. We measured salivary cortisol levels in 73 college undergraduate students during a 60-min interaction period with a dog to determine whether human-canine interactions are effective in lowering cortisol levels and potentially reducing chronic stress typical of undergraduate students. Our results indicate that a human-canine interaction for 60 min is an effective method for significantly reducing salivary cortisol and stress levels among undergraduate college students. These findings support the expansion of animal visitation programs on college campuses to help students manage stress.     

葛根素的心血管系统药理作用及机理研究

纵观近年来对葛根素在心血管系统药理作用及机理研究方面的进展发现，葛根素对心血管系统具有抗心律失常、降血压、扩张冠状动脉、抗心肌缺血、抗过氧化、抗缺血-再灌注损伤、影响血液动力学和血小板聚集、保护血管等作用。其临床应用前景良好，其药效机制研究及临床应用有待更深一步的探究。     

EFFECT OF ELECTROACUPUNCTURE OF SCALP-POINTS ON ABNORMAL DISCHARGES OF NEURONSAROUND THE CEREBRAL HEMORRHAGE FOCUS IN THE RAT

Thereexistdifferentdegreesofpathophysi ologicchangesinthetissuesaroundhemorrhagefocusafteracutecerebralhemorrhage[1 ] .Cere bralhemorrhageoftenleadstodysfunctionoftheneuronsinthebrain ,manifestingabnormalelec tricalactivity .Followingacutecerebralhemor rhage,duetotheoppressionofthehematomaandthetoxiceffectofthebloodcontentsaswellaschangesofthemicro environmentaroundthehemorrhagefocus,theelectricalactivityofthecerebralneuronsissuppressedatdifferentde grees.Electroacupuncture (EA)therapyworkell…     

电针对大鼠不稳定膀胱逼尿肌及膀胱颈氮能神经递质的影响

目的:观察电针治疗对大鼠不稳定膀胱模型下尿路组织氮能神经递质的影响。方法:制作大鼠不稳定膀胱模型,电针会阳、中膂俞7d后,采用免疫组织化学法检测大鼠膀胱逼尿肌、膀胱颈平滑肌神经源性一氧化氮合酶(neuronal nitric oxide synthase,nNOS)、内皮型一氧化氮合酶(endothelial nitric oxide syn-thase,eNOS)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)阳性细胞并计数。结果:不稳定膀胱模型其膀胱逼尿肌、膀胱颈平滑肌中氮能神经递质明显下降,而电针治疗则可促进氮能神经递质的生成和分泌。结论:电针促进膀胱组织中氮能神经递质的生成和分泌可能是电针治疗改善不稳定膀胱功能的主要机制之一。     

丹红注射液对糖尿病大鼠视网膜的保护作用

目的：观察丹红注射液对于糖尿病大鼠视网膜是否具有保护作用,并探讨其机制。

方法：从2015/06～2016/12共60只雄性 SD 大鼠随机分成4组：正常组、糖尿病组、丹红干预组以及空白干预组,后三组大鼠采用链脲佐菌素(STZ)50 mg/kg的剂量腹腔注射建立糖尿病视网膜病变大鼠模型。在大鼠建模成功后当日,丹红干预组每日腹腔注射5 ml/kg丹红注射液,空白干预组每日腹腔注射与丹红干预组等体积的蒸馏水。然后从视网膜血管病变和神经损伤两个层面进行疗效观察,血管病变层面行HE染色观察视网膜形态,免疫组化检测视网膜血管内皮生长因子的表达; 神经损伤层面行Tunel染色观察凋亡细胞,透射电镜观察视网膜神经节细胞形态。

结果：血管层面：正常组视网膜各组织结构显现清晰的层次,糖尿病组和空白干预组较正常组结构紊乱,丹红干预组较糖尿病组和空白干预组有所改善。与糖尿病组和空白干预组相比,丹红干预组VEGF表达降低; 神经损伤层面：糖尿病组和空白干预组神经节细胞出现大量凋亡,丹红干预组凋亡细胞较之减少。

结论：丹红注射液对糖尿病大鼠视网膜有保护作用。通过下调VEGF,改善视网膜缺血缺氧状态,减少视网膜新生血管的形成。同时能在一定程度上减少视网膜神经细胞的凋亡。     

曹仁发三步推拿法治疗失眠临床疗效观察

目的观察曹仁发教授的三步推拿法治疗失眠的临床疗效。方法将90例失眠患者随机分为安定组、推拿组和结合组(各30例),分别给予舒乐安定片口服和(或)曹仁发三步推拿法治疗,疗程12天。观察三组临床疗效和治疗前后匹兹堡睡眠质量指数量表(PSQI)评分、阿森斯失眠量表(AIS)评分和多导睡眠图结果变化。结果 (1)安定组总有效率为83.30%,推拿组为80.00%,结合组为93.30%;结合组临床疗效优于安定组和推拿组(P0.05)。(2)治疗后,三组PSQI和AIS评分均较治疗前明显降低(P0.05),且结合组低于安定组和推拿组(P0.05)。(3)治疗后,三组浅睡眠时间及深睡眠时间均较治疗前显著延长(P0.05),且结合组长于安定组和推拿组(P0.05)。结论曹仁发三步推拿法可显著改善失眠患者的睡眠质量。     

不同预后慢性疲劳综合征患者精神情志因素差别分析

目的:研究CFS患者精神情志因素与预后的关系。方法:运用SCL90量表及FS-14量表对120例未干预治疗CFS患者进行测评并进行3个月随访,分析精神情志因素与患者预后的关系。结果:未干预治疗CFS患者中有70.83%的患者症状未见好转,好转组评分与未好转组SCL-90评分有统计学差异(P<0.05)的因子分有:躯体化、敌对、抑郁、焦虑及总分。结论:(1)未干预治疗CFS患者预后不佳,高达70%以上的患者在未经适当治疗的情况下持续存在各种精神情志症状。(2)当CFS患者存在明显自觉躯体症状、敌对、强迫、抑郁、焦虑等负面情绪时其预后多不佳。     

MiR-130b attenuates vascular inflammation via negatively regulating tumor progression locus 2 (Tpl2) expression

Endothelial cell (EC) activation and dysfunction have been linked to a wide variety of vascular inflammatory diseases. However, the role of microRNAs in EC activation and inflammation remains largely unknown. In this study, we found that miR-130b was significantly decreased in human umbilical vein endothelial cells (HUVECs) after lipopolysaccharides (LPS) treatment. Forced expression of miR-130b inhibited the LPS-induced activation of extracellular signal-regulated kinase (ERK) and the inflammatory genes expression, such as interleukin (IL)-6 and tumor necrosis factor alpha (TNF-α). Furthermore, we identified that tumor progression locus 2 (Tpl2) is a direct target of miR-130b. Finally, in vivo overexpression of miR-130b via miR-130b agomir attenuates acute lung vascular inflammation in the LPS-induced sepsis mouse model. Taken together, our data demonstrated that miR-130b represses vascular inflammation via targeting Tpl2, suggesting that miR-130b mimics might be a promising therapeutic strategy for treatment of vascular inflammatory diseases.