不同训练时段影响甲基苯丙胺诱导的大鼠自身给药行为的形成

目的 观察不同时间给予甲基苯丙胺(METH)对大鼠形成自身给药行为的影响。方法 通过6 d的FR1程序训练合格的大鼠进入实验。按照4个给药时间8:00-8:30,10:00-10:30,11:30-12:00及16:00-16:30和2个给药因素生理盐水和METH 0.05 mg·kg^-1分为8组,持续8 d。在自身给药装置中FR1程序记录给药次数和有效鼻触和无效鼻触次数。结果 与同一给药时间的溶剂对照组相比,METH组大鼠自身给药的次数及有效鼻触次数显著增加(P<0.05)。不同时间段给予METH组的给药次数和有效鼻触次数均没有显著性差异,但是从8 d的给药次数来看,分别为8:00-8:30组(86.2±23.8)<10:00-10:30组(104.3±41.3)<11:30-12:00组(123.4±50.3)<16:00-16:30组(155.0±51.7)。从前4 d给药时间8:00-8:30改为后4 d给药时间11:30-12:00后,给药次数和有效鼻触次数明显增加(P<0.05);从前4 d给药时间11:30-12:00改为后4 d给药时间8:00-8:30后,给药次数和有效鼻触次数明显减少(P<0.05)。8组大鼠间无效鼻触次数没有显著差异。结论 10:00-17:00时间段进行训练有利于大鼠自身给药行为的形成。     

化红胶囊对去势大鼠骨质疏松的影响

目的:探讨化红胶囊对去势大鼠骨质疏松的影响及作用机制。方法:取3月龄SD雌性大鼠经去势手术或者是假阳性手术后,喂以去大豆特殊饲料7周以诱导骨丢失。经去势手术大鼠进一步分为化红胶囊组(1.0,0.5,0.25 g.kg-1)、模型组、己烯雌酚组,连续给药12周。检测大鼠血清的骨钙素、雌二醇、尿中脱氧吡啶啉含量,以及椎骨末端骨小梁的变化情况。结果:化红胶囊能降低大鼠血清中骨钙素以及尿液中脱氧吡啶啉含量,提高血清中雌二醇含量,与模型组相比,1.0g.kg-1,0.5 g.kg-1剂量组差异有显著性(P<0.05或P<0.01)。骨小梁的形态学观察及数据分析显示,与模型组相比,化红胶囊可以提高骨小梁面积百分比,与模型组相比,1.0,0.5 g.kg-1剂量组差异有显著性(P<0.05或P<0.01)。结论:化红胶囊可以降低去势大鼠骨转换率和骨吸收程度,提高骨小梁平均面积百分比,是一种潜在的治疗绝经后骨质疏松药物,其作用机制之一可能与化红胶囊雌激素样作用有关。     

食管鳞癌中VEGF-C、VEGF-D的表达与临床病理特征的相关性研究

目的 检测血管内皮生长因子-C、D(VEGF-C和VEGF-D)在食管鳞癌中的表达与各临床病理特征之间的关系,探索食管鳞癌淋巴结转移的机制.方法 免疫组化法检测VEGF-C、D在62例食管鳞癌根治术后标本及20例癌旁正常组织中的表达,计数微淋巴管密度(MLD).分析VEGF-C、D表达及MLD与临床病理特征之间的关系.结果 肿瘤组织中VEGF-C和VEGF-D的高表达率分别是74%(46/62)和82%(51/62);肿瘤边缘MLD(5.387±4.986)明显高于癌旁正常组织(1.900±2.245)(P<0.05).VEGF-C、D高表达组与低表达组相比,淋巴结转移更常见、临床分期也较晚,MLD增加与VEGF-C、D高表达、有淋巴结转移及临床分期较晚有关.VEGF-C和VEGF-D的表达有正相关关系(r=0.691,P<0.001).结论 VEGF-C和VEGF-D在食管鳞癌中可能通过增加肿瘤周边区域的淋巴管新生促进肿瘤的淋巴道转移.     

改良显微小梁切除术治疗葡萄膜炎继发性青光眼

Objective To investigate the effect of trabeculectomy combined with segmental iridectomy, mitomycin C (MMC) and viscoelastic agents usage on the treatment of glaucoma secondary in uveitis. Methods According to the age, degree of inflammation and the condition of Tenon capsule of patients, differ-ent concentration of MMC (0.25-0.33 mg/ml) was used during the operation, with separation of the anterior and posterior synechia, resection of pupillary organization membrane using viscoelastic agents. Segmental iridec-tomy and releasable sutures were also performed on the patients. The visual acuity of preoperation and postoper-ation, intraocular pressure, inflammation and the complication were record. Results Forty-two eyes of 38 cases with glaucoma secondary in uveitis were studied, the mean follow-up time was (12.01±3.56) months. The postoperative visual acuity improved in 14 eyes, didn't change in 28 eyes. The postoperative inflammation of anterior chamber disappeared in 35 eyes, relieved in 7 eyes. And the average postoperative intraocular pres-sure (15.20± 4.64) mmHg was significantly lower than the preoperative intraocular pressure (38.37±12.93) mmHg (t = 8.255, P = 0.000). The total success rate was 92.9%. There were no severe complication. Conclusion Trabeculeetomy combined with MMC, viscoelastic agents usage, separation of anterior and poste-rior syneehia, segmental iridectomy and releasable suture could increase the success rate of operation on pa tients with glaucoma secondary in uveitis, decrease the complication and inflammation reaction of operation, and the recurrence of uveitis.     

益气温阳补肾汤、常规西药联合治疗肾病综合征(NS)临床疗效分析

目的:分析益气温阳补肾汤、常规西药联合治疗肾病综合征(NS)的临床疗效.方法:随机选择100例NS患者作为研究对象,平均分为两组,对照组50例NS患者接受常规西药治疗,观察组接受益气温阳补肾汤联合常规西药治疗,对比临床疗效.结果:治疗后,观察组NS患者治疗效果和临床指标均优于对照组,(P<0.05).结论:治疗肾病综合征患者采用益气温阳补肾汤联合常规西药能够取得理想的治疗效果,不仅促进治疗有效率提升,在很大程度上改善了患者的临床指标,消除了炎症,提高了身体免疫力.     

改良显微小梁切除术治疗葡萄膜炎继发性青光眼

Objective To investigate the effect of trabeculectomy combined with segmental iridectomy, mitomycin C (MMC) and viscoelastic agents usage on the treatment of glaucoma secondary in uveitis. Methods According to the age, degree of inflammation and the condition of Tenon capsule of patients, differ-ent concentration of MMC (0.25-0.33 mg/ml) was used during the operation, with separation of the anterior and posterior synechia, resection of pupillary organization membrane using viscoelastic agents. Segmental iridec-tomy and releasable sutures were also performed on the patients. The visual acuity of preoperation and postoper-ation, intraocular pressure, inflammation and the complication were record. Results Forty-two eyes of 38 cases with glaucoma secondary in uveitis were studied, the mean follow-up time was (12.01±3.56) months. The postoperative visual acuity improved in 14 eyes, didn't change in 28 eyes. The postoperative inflammation of anterior chamber disappeared in 35 eyes, relieved in 7 eyes. And the average postoperative intraocular pres-sure (15.20± 4.64) mmHg was significantly lower than the preoperative intraocular pressure (38.37±12.93) mmHg (t = 8.255, P = 0.000). The total success rate was 92.9%. There were no severe complication. Conclusion Trabeculeetomy combined with MMC, viscoelastic agents usage, separation of anterior and poste-rior syneehia, segmental iridectomy and releasable suture could increase the success rate of operation on pa tients with glaucoma secondary in uveitis, decrease the complication and inflammation reaction of operation, and the recurrence of uveitis.     

颈部原始神经外胚叶瘤1例

1病例资料患者,女性,13岁,因“发现左颈包块2月”入院。患者在2月前无意中发现左颈有一4cm×3cm大小包块,质硬固定,无疼痛,在当地医院穿刺涂片示“炎症”,经抗炎治疗包块稍缩小。20天前包块进行性长大伴红肿疼痛,在当地医院行包块切取活检示“小细胞恶性肿瘤,倾向胚胎型横纹肌肉瘤”。于2004年11月29日入院,入院时左颈部包块约8cm×9cm大小,活检组织切片经我院病理科会诊后,诊断:(左颈)原始神经外胚叶瘤(高度恶性)。免疫组化:CD99(+),NSE(+),Desmin(-),Myogenin(-),LCA(-),MPO(-),S-100(-)。于2004年12月5日开始全身化疗第一周期(异环…     

间充质干细胞与炎症性肠病的研究进展

间充质干细胞(MSC)是一种具有多分化潜能的细胞,还具有免疫调节功能。炎症性肠病(IBD)的病因和发病机制目前尚未完全明确,近年研究显示免疫紊乱在IBD的发病中起重要作用。MSC的免疫调节特性为治疗IBD提供了一种潜在的可能性。本文就MSC的免疫调节效应及其在IBD中的作用作一综述。     

登革病毒基因组核苷酸变异与毒力的关系

登革病毒是一种流行于热带、亚热带地区的蚊媒病毒，其引起的登革热、登革出血热／登革休克综合征（DSS／DHF）已成为严重威胁人类健康的公共卫生问题。登革病毒缺乏精确的复制校正系统．在传播过程中核苷酸的变化会导致病毒毒力的变化，目前其致病与免疫机制尚未明了，缺乏有效的疫苗。本文就登革病毒基因变异与其毒力关系方面的研究进行综述。