RPMI1640培养液在阴道滴虫培养中的应用

目的：评价１６４０培养液在阴道滴虫体外培养中的应用效果。方法；在ＲＰＭＩ１６４０培养液中加入其它在份，配制成新的阴道滴虫培养基。观察其对阴道滴虫的培养效果，并与ＣＰＬＭ培养基作比较。结果：滴虫在两种培养基中的增殖倍数有显著性差异。在临床标本中分离方面与ＣＰＬＭ培养基无明显差异，但在保种时间上比ＣＰＬＭ培养基较长。结论：ＲＰＭＩ１６４０培养液应用于阴道滴虫培养中效果良好。     

胆固醇脂质体对培养兔胆道口括约肌细胞的增殖作用及其意义

通过胆固醇脂质体对培养兔胆道口括约肌细胞作用的研究，以探讨高胆固醇血症ＳＯ功能形态改变间的关系，影响因素及高胆固醇血症诱发胆固醇结石形成的可能机理。方法；分离幼兔胆道口括约肌行原代培养，经免疫组化及电镜诱发，给予人工ＣＬ，分别采用ＤＭＥＭ和ＲＰＭＩ－１６４０培养基，行细胞增殖四唑盐比色实验及流式细胞仪ＦＣＭ分析。     

兔晶体上皮细胞在不同材料人工晶体表面粘附密度的实验研究

利用体外培养的兔晶体上皮细胞（ＬＥＣ），制成４×１０４个／ｍｌ的细胞悬液接种于国内生产的３种不同材料人工晶体（ＩＯＬ）表面，在ＣＯ２培养箱内孵育２４ｈ（３７℃），再将粘附于ＩＯＬ表面的细胞消化下来、计数。结果：粘附于ＰＭＭＡＩＯＬ表面的细胞数最多，与其在ＳＩ、ＨＥＭＡＩＯＬ表面的细胞数比较差异显著（Ｐ＜０．０５）；ＳＩ与ＨＥＭＡ之间差异不显著（Ｐ＞０．０５）。     

表皮生长因子受体反义基因转染对人恶性胶质瘤细胞体外生长的影响

目的探讨表皮生长因子受体（ＥＧＦＲ）反义基因转染对人恶性胶质瘤细胞Ｕ８７ＭＧ体外生长的影响。方法构建ＥＧＦＲ反义基因表达载体，应用脂质体转染法将其导入Ｕ８７ＭＧ细胞；ＰＣＲ方法鉴定转染克隆；Ｗｅｓｔｅｒｎ印迹杂交检测ＥＧＦＲ蛋白表达水平；通过绘制生长曲线和软琼脂集落形成实验测定反义ＥＧＦＲ基因对恶性胶质瘤细胞体外生长的抑制作用。结果转染反义ＥＧＦＲ基因的细胞克隆株ＡＳ１ＡＳ６的ＥＧＦＲ蛋白表达水平均有不同程度的降低；ＡＳ１ＡＳ６细胞体外生长明显减慢，ＡＳ１ＡＳ６细胞克隆形成率明显降低。表明Ｕ８７ＭＧ细胞体外生长速度和恶性转化能力均与ＥＧＦＲ表达呈正相关。结论反义ＥＧＦＲ基因转染可以明显抑制Ｕ８７ＭＧ细胞生长，ＥＧＦＲ对Ｕ８７ＭＧ细胞生长具有重要的调控作用     

白细胞介素—6对急性白血病原代细胞的影响及意义

用ＲＰＭＩ１６４０培养基，在不同浓度的白细胞介素－６条件下对原代白血病细胞进行 外培养，用ＭＴＴ法测细胞增殖提数，     

人脑胶质瘤浸润淋巴细胞的分离培养与活性测定

目的 分离、培养人脑胶质瘤肿瘤浸润淋巴细胞并对其进行活性测定，探讨临床应用肿瘤浸润淋巴细胞的理论根据。方法 采用机械分离、酶解聚和不连续密度梯度离心方法从１０例脑胶质瘤制备肿瘤浸润淋巴细胞单细胞悬液，体外培养扩增；采用ＮＡＧ酶荧光比色法测定肿瘤浸润淋巴细胞杀伤活性。结果 肿瘤浸润淋巴细胞得率达到７２．５±３．２１；在ＲＰＭＩ１６４０完全培养基中生长旺盛，第３、４周能够达到１０＾９数量级，此时杀伤活     

培养的肺动脉内皮细胞生长及生化特性的初步观察

采用胰蛋白酶消化法和刮取法培养了肺动脉内皮细胞（ＰＡＥＣ）。通过原代培养、传代和冻存复苏等步骤，观察了ＰＡＥＣ的形态及生化特点，如Ⅷ因子相关抗原、细胞内钙、环磷酸腺苷等。结果表明：原代及传代培养的ＰＡＥＣ可很快贴壁生长，经２￣５ｄ可汇合成单层，细胞呈铺路石状排列，Ⅷ因子相关抗原阳性，并能分泌血管紧张素转移酶；在含２０％小牛血清的ＲＰＭＩ－１６４０培养基内，ＰＡＥＣ可传３０代以上；传代后的细胞生长速     

人脑胶质瘤肿瘤浸润淋巴细胞的分离培养与活性测定

目的分离、培养人脑胶质瘤肿瘤浸润淋巴细胞并对其进行活性测定，探讨临床应用肿瘤浸润淋巴细胞的理论根据。方法采用机械分离、酶解聚和不连续密度梯度离心方法从１０例脑胶质瘤制备肿瘤浸润淋巴细胞单细胞悬液，体外培养扩增；采用ＮＡＧ酶荧光比色法测定肿瘤浸润淋巴细胞杀伤活性。结果肿瘤浸润淋巴细胞得率达到７２５±３２１；在ＲＰＭＩ１６４０完全培养基中生长旺盛，第３、４周能够达到１０９数量级，此时杀伤活性亦达到高峰。结论肿瘤浸润淋巴细胞适当条件体外培养扩增，在数量和杀伤活性等方面能够满足临床要求，培养第３、４周是肿瘤浸润淋巴细胞应用的时机。     

不同压力对兔动脉平滑肌细胞增殖的调控作用研究

目的：探讨不同压力状态对动脉平滑肌增殖的调控作用,由此判断冠脉再狭窄过程中压力因素所起的作用。方法：用含１０％小牛血清的ＲＰＭＩ－１６４０培养液体外培养新西兰白兔的主动脉平滑肌细胞至３－７代,将含５％小牛血清的ＲＰＭＩ－１６４０培养液与动脉平滑肌细胞的混悬液１００μｌ加入９６孔培养板中培养。２４ｈ后按照实验分组分别在６．６５ｋＰａ,１３．３ｋＰａ,１９．９５ｋＰａ,２６．６ｋＰａ压力状态下持续培养     

用漏出液代替标准培养基培养人体外周血淋巴细胞

用漏出液代替标准培养基培养人体外周血淋巴细胞生物学教研室肖桂芝，刘朝晖，冯立新关键词培养基，淋巴细胞，有丝分裂指数培养人体外周血淋巴细胞一般是用Ｍ１９９、ＲＰＭＩ１６４０、ＨａｍＦ１０等标准培养基，但标准培养基均由国外购进，价格比较昂贵，不利于基层及...     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…