自然周期未成熟卵体外培养、受精和胚胎移植研究

目的:探讨自然周期成熟卵体外成熟培养(In vitromaturation,IVM)、受精和胚胎移植技术在治疗不育症中的应用.方法:选取30对接受体外授精/卵胞浆内单精子显微注射-胚胎移植(In vitrofertilization/intracytoplasmic sperminjection-embryotransfer,ⅣF/ICSI-ET)助孕的小育夫妇为对象,在自然月经周期卵泡直径>10 mm、内源性LH(Luteinizing hormone)峰出现之前采卵.采卵当日成熟卵(Metaphase Ⅱ,M Ⅱ)行IVF授精,未成熟卵进行体外培养24～48 h,成熟至M Ⅱ期后行ICSI授精,受精后72 h行胚胎移植.结果:30个ⅣM周期中,2个周期取卵末获卵.另28个周期共获卵113个,其中成熟卵29个,退化卵10个,未成熟卵74个,体外培养后成熟印个,成熟率81.08%.20个周期有胚胎移植,6例临床妊娠.其中5例成功分娩足月健康婴儿(1例来自成熟卵、4例来自未成熟卵),1例临床妊娠流产.结论:自然周期未成熟卵IVM、受精和胚胎移植技术可以用于治疗不育患者.     

恒河猴(Macaca mulatta)卵细胞采集及体外受精的研究

对13头成年母猴,用黄体酮 前列腺索(PGF_(2a))处理,诱发月经周期,并用三种不同的处理方案,注射外激性促性腺激素诱发等超数排卵。对171个大滤泡(直径≥4mm)实行外科手术采卵,滤泡穿刺吸卵共获147个卵细胞,平均每头猴采卵11.31±6.47个,卵细胞回收率为85.96％。精液用直肠电刺激法采集,精子经TALP培养基洗涤二次,并在含10％灭活猴血清的TALP液中,加入1mM CAMP和lmM咖啡因,预孵育6～3h。卵细胞在含10％灭活猴血清的TALP或Ham''sF-10培养基中作体外成熟的培养,经约4～12h孵育,卵细胞体外成热率为28.57％。成熟的卵细胞(n=42,中期Ⅱ)和已预孵育的精子一起12～24h,并有部分在前次输精后5～12h再次输精,在42～56h后有9个受精卵继续发育至2细胞期和桑椹期阶段,体外受精率为35.71％(15/42)。     

人工授精和体外授精

生儿育女是家庭生活的一个重要组成部分,然而在所有结婚的夫妇中大约有10％的夫妇由于某种原因而无法获得小孩,为了解决这些人的烦恼,人工授精与体外授精技术便应运而生。 人工授精是用人工的方式将精子注入子宫内,促使女性怀孕;体外授精是以人工方式将取自母亲的卵子和取自父亲的精子先在试管内混合授精,之后再将授精卵移植到子宫内使它着床。两种授精方式相比,体外授精比人工授精在技术操作上要复杂得多,手术的第一步是排卵前卵子的吸取,在这个步骤中妇女必须全身麻醉,并由医生以腹腔镜在完全无菌的手术室中进行。第二步是将取出的数个卵子按不成熟     

体外保存的家兔精子在体内的受精功能

虽然哺乳动物的精子可以在体外妥善保存,但尚不知这样的精子是否能在辅卵菅中受精,也不知道它们的受精能力是否与保存在体内的精子相同.本文用保存过的精子进行体外受精(方法前已报道,即在37℃,380mOsm/千克的高离子强度的培养剂HIS中置放20分钟),结果精子不能在输卵菅中渗入卵细胞,除非让精子在雌兔输卵管中有较长时间的适应过程.因此,尽管精子可能适应保存的环境,HIS处理显然是不能完成这一过程的.然而,精子在另一组限定条件下培养12小时(37℃,无牛血蛋白培养剂 20%在油下用5%CO_2与8%O_2平衡过的血清),将此种精子输入12——15小时后检查,在14次试验中,有7次使卵细胞受精.虽然如此,体外保存了12小时的精子,其授精能力(131个卵的29%受精;0.3卵周精子/卵(比在对侧输卵管中的子宫内精子要小10倍(43个卵的81%受精;15个卵周精子/卵).而且经过后一种处理过的精子,若与许多没有处理过     

体外授精胚胎移植后双侧输卵管同时妊娠1例

患者,女,29岁,4年前自然流产1次,因双侧输卵管梗阻入我中心行体外授精-胚胎移植术,采用长方案进行控制性超排,体外授精后培养2天,于2005年4月11日选择3个胚胎(8/Ⅰ×2,6/Ⅱ×1),在超声引导下于距宫底1cm处植入胚胎,术后平卧6小时。移植后15天查尿HCG( ),继续黄体支持治疗。12天     

体外受精—胚胎移植治疗不孕症64个周期的观察研究

目的　分析本中心开展体外授精 -胚胎移植 (IVF- ET)治疗不孕症 6 1例 6 4个周期的治疗结局。方法　接受治疗患者年龄 2 5岁至 41岁 ,共进行 6 4个周期的常规 IVF- ET治疗 ,于取卵后 48～ 5 0 h进行胚胎移植。结果　本组治疗周期 6 4个 ,取消周期 4.6 % ,取卵周期 6 1个 ,平均周期取卵数 (9.6 1± 6 .6 3)个 ,受精率 71.32 % ,卵裂率 90 .78% ,每周期胚胎移植数 (3.86± 1.0 9)个 ,临床妊娠率为 2 8.0 7%。结论　 IVF- ET治疗输卵管梗阻性及原因不明不孕症在临床已获成功 ,提高妊娠率有待改善各种有关条件     

染色体和遗传

950071人卵、受精那及前期狂胎染色体初步分析刘平一产中华妇产科杂志一19叫.2,(1如一功~·12 应用36例妇女进行体外授精一胚胎移值研究淘汰的未受精卵,未分裂的受精卯及形态不好的前期胚胎,制成染色体标本44个,进行染色休分析。结果:正常未受精卵标本13个.染色体数目正常.卵母细咆处于第二次减数分裂中期,体外授精失败,结合病史分析,与精子质量和卵成熟度有关;异常未受精标本4个,正常卵受精标本7个.染色体数目正常的卵母细胞虽受精,但有的未抛出第二极体,精卵发育不同步;异常卵受精标本8个,染色体数目异常的卵母细胞虽受精,但发育停滞,多精…     

应用特异性McAb被动免疫和治疗恒河猴实验性脊髓灰质炎病毒感染的研究

将21只健康恒河猴随机分成两组,分别用Polio病毒Ⅱ型MEF_1株小剂量(2×10~(7.5)TCD_(50))及大剂量(20×10~(7.5)TCD_(50))经腓肠肌感染。治疗组猴在感染后24小时静注10万～30万效价/公斤体重的抗Polio Ⅱ型病毒McAb(小鼠腹水)。感染前及感染后21天内定期采猴血2～3毫升,用原代猴肾细胞分离病毒及测定血清抗体效价,并作临床观察。感染后21天剖杀,进行组织病理学检查。初步实验结果:①小剂量病毒组中有2/6对照猴发生临床麻痹,治疗猴无一麻痹,大剂     

阔腹猴虱在幼年猕猴中的寄生及药物治疗

1991年在对本中心264只1岁半左右的幼年猕猴常规检疫中,发现37只感染了阔腹猴虱。本文报告对这批幼猴感染虱的调查情况,及以速灭杀了治疗的效果。材料与方法 1.动物 264只自繁幼年猕猴,1岁半左右;分群饲养在28个笼中,每笼7～14只,已达1年。 2.方法在常规检疫中逐只对幼猴进行体外寄生虫检查。发现有虱感染者,观察记录虱分布部位、虱及卵寄生密度。采集猴虱成虫及卵,并送贵阳医学院金大雄教授鉴定。以1％速灭杀丁浸浴已感染幼猴(除面部外)。7d后,检查药浴效果。结果 1.猴虱感染率 264只幼猴分养于28个猴笼中。其中3个笼的幼猴(37只)全部感染虱,而其它25笼幼猴则无1只感染。感染率     

食蟹猴疟原虫B株—斯氏按蚊—恒河猴猴疟模型在国内的建立

我们于1985年引进食蟹猴疟原虫B株,观察其生物学特性,建成食蟹猴疟原虫B株—斯氏按蚊—恒河猴猴疟模型。B株原虫对斯氏按蚊感染性强,斯氏按蚊叮咬输血感染后7～11天的猴,其卵囊阳性率可达80～100％;第8天卵囊成熟,第10天子孢子进腺率平均52.7％。猴感染后第7天肝切片中红外期原虫不分叶,无     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.