麦冬多糖水提醇沉工艺研究

目的为麦冬多糖的进一步开发和利用提供参考。方法采用水提醇沉法,对麦冬多糖进行提取;苯酚-硫酸法测定多糖含量;采用L9(34)正交试验,以多糖百分含量为指标,对实验结果进行极差和方差分析。结果通过对正交实验进行极差、方差分析,确定了麦冬多糖最佳提取工艺条件为加8倍量水,提取3次,每次1.5小时,醇沉浓度为60%,用苯酚—硫酸法测定多糖含量为43.162%。结论正交实验表明醇沉浓度对麦冬多糖提取率有显著影响,其次为提取次数和提取时间。     

山药多糖提取工艺的研究

目的：对山药多糖水提醇沉工艺进行研究,得出山药多糖的最佳提取工艺。方法：以山药总多糖为指标,采用分光光度法,单因素考察及正交设计试验法,对料液比、提取温度、提取时间、提取次数进行考察;并对醇沉浓度、醇沉时间和醇沉次数进行考察。结果：水提正交试验结果表明,料液比1：10,温度100℃,时间4h,提取3次为最佳工艺条件。醇沉正交试验表明,醇沉后溶液浓度为80%,时间为12h,醇沉1次。结论：该实验为山药多糖提取工艺的确定提供依据。     

党参多糖的提取工艺研究

目的：确定党参多糖的最佳提取工艺。方法：以多糖提取率为指标,用正交实验法确定党参多糖的最佳提取工艺和醇沉条件。结果：最佳提取工艺料液比为1∶10,提取1.0 h,提取3次;醇沉条件为：提取液浓缩至1 g/mL,加入乙醇至醇沉浓度为80%,醇沉时间为12 h。结论：该工艺简便、稳定、准确,可用于党参多糖的提取。     

正交试验法优化鱼腥草多糖水煎煮提取工艺

目的:优选鱼腥草多糖的提取工艺。方法:以多糖得率、多糖含量为指标成分,采用正交试验对鱼腥草多糖的提取工艺进行优选。结果:优化工艺为:90℃水温浸3次,每次2h,醇沉浓度为80%。多糖得率及含量分别为24.7%、27.9%。结论:该工艺合理,多糖成分提取完全,具有较好的经济效益。     

金银忍冬多糖提取工艺研究

目的：研究金银忍冬中多糖的最佳提取工艺。方法：采用正交试验确定各因素的影响大小,确定金银忍冬中多糖的最佳提取条件,用水提醇沉方法提取多糖,苯酚-浓硫酸法测定多糖含量。结果：温度对金银忍冬多糖提取影响作用最大,金银忍冬多糖的最佳提取工艺：温度为90℃;提取3次,3h/次。结论：该研究可为金银忍冬多糖工业化提取提供理论依据。     

云芝多糖对人CIK细胞及其杀伤功能相关分子表达的影响

目的 探讨云芝多糖对人CIK细胞增殖及细胞杀伤功能相关分子表达的影响.方法 体外培养CIK细胞,不同浓度云芝多糖诱导人CIK细胞24h,CCK-8比色法检测人CIK细胞增殖,流式细胞仪测定CIK细胞表达的穿孔素、颗粒酶B和CD107a的变化.结果 不同浓度的云芝多糖可促进CIK细胞增殖,促进CIK细胞穿孔素、颗粒酶B的和CD107a的表达,25 mg/L云芝多糖作用最强;但随着药物浓度的增加,CIK细胞增殖率逐渐降低,且细胞杀伤功能相关分子表达含量下降.结论 云芝多糖在低浓度下可促进CIK细胞增殖,提高CIK细胞的杀伤活性和功能.     

茯苓多糖提取工艺研究

目的:优选茯苓水溶性粗多糖的水提醇沉工艺。方法:以茯苓水溶性多糖提取率为参察指标,以水为溶剂,选取粉碎力度、料液比、提取时间、提取次数、醇沉浓度、浓缩倍数、醇沉时间为影响因素,进行单因素考察,在综合单因素实验的基础上,采用L9(34)正交试验对水提和醇沉工艺进行优化设计,采用硫酸-蒽酮法测定多糖的含量。结果:茯苓水溶性多糖最佳的水提醇沉工艺为:过60目筛、料液比为1∶10,提取时间为100 min,提取1次,提取液浓缩至原体积的1/12,醇沉浓度为80%,静置12 h,过滤,滤饼用体积分数80%乙醇洗涤,60°C减压干燥。结论:此工艺成本低、操作简便、重现性好,可用于茯苓药材及饮片粗多糖的提取。     

正交设计优选鳖甲多糖提取工艺的研究

目的 确定鳖甲多糖的最佳提取工艺,以苯酚硫酸法测定鳖甲多糖含量。方法 采用碱提法提取、醇沉得到鳖甲多糖;利用苯酚硫酸法显色,在490 nm处测定吸光度。结果 碱液浓度为8%、料液比为1∶25、提取温度为60 ℃、提取时间8 h为最佳提取工艺,最佳醇沉浓度为80%;平均回收率为100.2%,RSD为1.4%(n=9)。结论 本实验鳖甲多糖提取及含量测定操作简便、结果准确、重复性好,可用于鳖甲多糖的提取与含量测定。     

桂枝解郁方的提取与精制工艺研究

目的:确定桂枝解郁方的水提醇沉的最佳工艺条件。方法:以芍药苷含量、浸膏得率为指标,采用正交试验对水煎工艺条件进行优选;以芍药苷含量为指标,考察了醇沉的条件。结果:水煎最佳工艺为加10倍量的水,煎煮2次,每次1.5h;醇沉工艺为:将水煎液浓缩成1.10(26℃)的清膏,加乙醇使含醇量达60%,搅匀,静置24h,滤过。结论:确定的提取工艺活性成分转移率高,精制工艺能最大程度保留活性成分。     

黄花菜多糖的超声提取工艺研究

目的探索黄花菜中粗多糖的超声提取工艺。方法黄花菜干粉经脱脂,超声波提取,Sevag法除蛋白,醇沉,真空抽滤,得到黄花菜粗多糖。从超声频率、提取温度、提取时间、料液比4个方面对提取率进行了分析。在单因素试验的基础上通过正交试验筛选出最佳提取工艺条件。用苯酚-硫酸法测定多糖的含量。结果正交试验得出黄花菜多糖的最佳提取工艺为水料比1∶20,超声功率60 Hz,提取温度60℃,提取时间15 min,该条件下黄花菜中粗多糖含量为9.67%。结论采用超声方法提取多糖节省时间,提取多糖的含量高。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.