携带IL-1B等位基因2牙周炎患者基础治疗后牙周可疑致病菌的变化

【目的】了解携带IL-1B（＋3954）等位基因2的牙周炎患者在牙周基础治疗后龈下菌斑中牙周可疑致病菌的动态变化情况。【方法】选择了携带IL-1B（＋3954）等位基因2牙周炎患者的42颗患牙为实验组（B2＋group）,无携带该等位基因牙周炎患者的40颗患牙为对照组（B2-group）。收集两组患牙在牙周基础治疗前、治疗后1、3、6个月时牙周临床指标菌斑指数（PI）、牙龈指数（GI）和探诊深度（PD）,采集龈下菌斑,采用细菌培养鉴定方法测定牙周可疑致病菌的检出率和检出量（CFU/g）。【结果】（1）在同一观察时间点,两组的临床牙周各项指标无统计学差异。（2）基线时,实验组和对照组的牙周可疑致病菌检出率无统计学差异,而实验组的梭杆菌、类杆菌和BPAR检出量则较对照组高（P〈0.05）。（3）基础治疗后1个月,两组的检出率和检出量明显下降（P〈0.05）。（4）治疗后3个月时,两组的可疑致病菌检出率和检出量较1个月均有上升,且实验组的BPAR检出率和检出量均较对照组高（P〈0.05）。（5）治疗后6个月时,两组的检出率基本回复基线水平;而实验组的真杆菌、梭杆菌、类杆菌和BPAR检出量则较对照组高（P〈0.05）. 【结论】（1）对于PD≥6mm的牙周炎患牙，基础治疗只能暂时控制牙周可疑致病菌。（2）基础治疗前后，携带IL-1B（＋3954）等位基因2患者的牙周深袋处都可检出有更多的梭杆菌、类杆菌和BPAR。     

IL-1B基因多态性与汉族慢性牙周炎临床指标的相关性

【目的】了解携带不同IL-IB 3954等位基因的汉族慢性牙周炎患的龈沟液中的IL-1β浓度、牙槽骨吸收程度、附着水平等相关临床牙周指标的差异情况。【方法】将已用PCR和限制性酶切方法检测IL-IB 3954等位基因情况的109名汉族慢性牙周炎患按其牙周情况分为轻度组(n=22)、中度组(n=48)、重度组(n=39)，在各组内分析比较不同IL-IB等位基因患的龈沟液中IL-IB浓度(ELISA法)、牙槽骨吸收程度、附着水平、探诊深度等临床指标的差异。【结果】携带IL-IB 3954等位基因2(B2阳性)患的龈沟液IL-1β浓度高于B2阴性患，其牙周破坏程度也较B2阴性患严重。【结论】汉族慢性牙周炎患的牙周破坏程度与患是否携带1L—1B( 3954)等位基因2呈正相关关系，该等位基因可能是发展为重度慢性牙周炎的危险因素。     

牙周基础治疗从不同途径对伴中重度牙周炎冠心病患者的影响

目的比较牙周基础治疗后伴有中重度慢性牙周炎的冠心病患者血清促炎因子水平及牙周可疑致病菌检出率的变化,分析牙周基础治疗对冠心病发生风险的影响．方法伴有中重度慢性牙周炎的冠心病患者80例,随机分为心内科常规治疗及牙周治疗组（实验组）和心内科常规治疗组（对照组）,8周后比较2组患者血清促炎因子水平及牙周可疑致病菌的检出率变化．结果2组患者牙周可疑致病菌检出率明显下降（P〈0．05）,实验组血清IL-1β及TNF-α水平明显降低（P〈0．01）．结论牙周基础治疗能从不同途径降低冠心病的发生风险,有利于冠心病的防治．     

IL-1B基因多态性与汉族慢性牙周炎临床指标的相关性

[目的]了解携带不同IL-1B 3954等位基因的汉族慢性牙周炎患者的龈沟液中的IL-1β浓度、牙槽骨吸收程度、附着水平等相关临床牙周指标的差异情况.[方法]将已用PCR和限制性酶切方法检测IL-1B 3954等位基因情况的109名汉族慢性牙周炎患者按其牙周情况分为轻度组(n=22)、中度组(n=48)、重度组(n=39),在各组内分析比较不同IL-1B等位基因患者的龈沟液中IL-1Β浓度(ELISA法)、牙槽骨吸收程度、附着水平、探诊深度等临床指标的差异.[结果]携带IL-1B 3954等位基因2(B2阳性)患者的龈沟液IL-1β浓度高于B2阴性患者,其牙周破坏程度也较B2阴性患者严重.[结论]汉族慢性牙周炎患者的牙周破坏程度与患者是否携带IL-1B( 3954)等位基因2呈正相关关系,该等位基因可能是发展为重度慢性牙周炎的危险因素.     

牙周牙髓联合病变与常见牙周病原微生物感染的关系研究

目的 分析牙周源性牙周牙髓联合病变患牙根管内口腔常见可疑致病菌的检出情况.方法 收集因牙周损害导致的牙周牙髓联合病变患牙30例(实验组),开髓揭顶冲洗后用纸尖采集根管内组织,抽提DNA;以5例因正畸拔除的健康前磨牙作为阴性对照组.采用二步PCR技术对可疑病原微生物进行检测,包括牙周可疑致病微生物福赛类杆菌(T.forsythensis)、牙龈卟啉单胞菌(P.gingivalis)、具核梭杆菌(F.nucleatum)、中间普氏菌(P.intermedia)、牙密螺旋体(T.denticola)和牙髓感染相关微生物牙髓卟啉单胞菌(P.endodontalis).结果 实验组患牙根管内组织样本中有13个样本存在目标微生物感染,总检出率为43.3%;其中检出T.forsythesis感染6例(20.0%).P,intermedia感染5例(17.0%),P.gingivalis感染4例(13.0%),P.endodontalis感染4例(13.0%),F.nucleatum感染1例(3.0%),所有样本中均未检出F.denticola.阴性对照组牙根管内组织样本中目标微生物检出率为零.结论 牙周源性牙周牙髓联合病变以牙周可疑致病微生物感染为主.     

牙周牙髓联合病变与常见牙周病原微生物感染的关系研究

目的分析牙周源性牙周牙髓联合病变患牙根管内口腔常见可疑致病菌的检出情况。方法收集因牙周损害导致的牙周牙髓联合病变患牙30例(实验组),开髓揭顶冲洗后用纸尖采集根管内组织,抽提DNA;以5例因正畸拔除的健康前磨牙作为阴性对照组。采用二步PCR技术对可疑病原微生物进行检测,包括牙周可疑致病微生物福赛类杆菌(T.forsythensis)、牙龈卟啉单胞菌(P.gingivalis)、具核梭杆菌(F.nucleatum)、中间普氏菌(P.intermedia)、牙密螺旋体(T.denticola)和牙髓感染相关微生物牙髓卟啉单胞菌(P.endodontalis)。结果实验组患牙根管内组织样本中有13个样本存在目标微生物感染,总检出率为43.3%;其中检出T.forsythensis感染6例(20.0%),P.intermedia感染5例(17.0%),P.gingivalis感染4例(13.0%),P.endodontalis感染4例(13.0%),F.nucleatum感染1例(3.0%),所有样本中均未检出T.denticola。阴性对照组牙根管内组织样本中目标微生物检出率为零。结论牙周源性牙周牙髓联合病变以牙周...     

腔治捷在治疗Ⅱ型糖尿病伴发牙周炎中的作用

【目的】观察牙周基础治疗联合腔治捷对II型糖尿病伴牙周炎的临床疗效。【方法】将30例慢性牙周炎伴II型糖尿病的患者,随机分成2组。实验组采用牙周基础治疗后应用腔治捷治疗,对照组牙周基础治疗后采用上1％碘甘油方法,分别于用药4、8周后观察临床效果。【结果】实验组与对照组均能有效的治疗II型糖尿病牙周炎。牙周治疗4、8周后,实验组与对照组菌斑指数（plaqueindex,PLI）差异均无统计学意义（P〉0．05）。牙周治疗4周后实验组较对照组的牙龈出血指数（suleusbleedingindex,SBI）下降更为显著（P〈0．05）,而牙周袋探诊深度（probingdepth,PD）、糖化血红蛋白（glycosylatedhemoglobin,HbAlc）差异无统计学意义（P〉0．05）。治疗8周后实验组PD、SBI、HbAIc与对照组指标比较差异均有统计学意义（P〈0．05）。【结论】腔治捷是慢性牙周炎伴II型糖尿病的安全有效的局部治疗药物。     

动脉粥样硬化斑块中牙周可疑致病微生物的检测

目的：检测动脉粥样硬化斑块中是否存在牙周致病菌。方法：共选取在手术过程中剥离的、已有动脉粥样硬化改变的动脉内膜标本10例,采用PCR扩增16S rDNA (16S ribosomal DNA)片段的方法,检测7种牙周可疑致病菌,包括牙龈卟啉单胞菌（Porphyromonas gingivalis, Pg）、福赛坦菌（Tannerella forsythia, Tf）、伴放线聚生杆菌（Aggregatibacter actinomycetemcomitans, Aa）、中间普氏菌（Prevotella intermedia, Pi）、变黑普氏菌(Prevotella nigrescen, Pn)、牙密螺旋体（Treponema denticola, Td）及直肠弯曲菌（Campylobacter rectus,Cr）。结果：10例标本中有3例检测出了牙周可疑致病菌,3例样本均检出了Pg,这3例中有1例同时检出了Tf,另1例同时检出了Pn;其余7例标本未检出相关牙周致病菌。结论：动脉粥样硬化斑块样本中检出了部分牙周可疑致病菌,为牙周炎与动脉粥样硬化之间可能存在联系提供了初步证据。     

白细胞介素-1B基因+3954C/T多态性与慢性牙周炎的相关性研究

目的 研究白细胞介素-1B(IL-1B)基因+3954C/T多态性与慢性牙周炎的关系.方法 采用病例对照实验设计,应用聚合酶链反应一限制性内切酶片段长度多态性基因分析方法 ,比较173例慢性牙周炎患者和168例牙周健康者IL-1B基因+3954位点基因型和等位基因分布特点.结果 IL-1B基因+3954位点CC、CT基因型检出率为96.5%和3.5%,无TT基因型,两种基因型和C、T等位基因频率在组间分布差异无统计学意义(P>0.05).结论 IL-1B基因+3954位点在中国汉族人群中变异率低,+3954位点的多态性与慢性牙周炎的易感性无关.     

2型糖尿病患者血糖控制对种植体龈沟牙周致病菌的影响

目的: 观察2型糖尿病患者的血糖控制对种植体龈沟牙周致病菌的影响。方法: 收集接受种植牙手术的106例2型糖尿病伴牙周炎的患者,按糖化血红蛋白（HbA1c）值分为HbA1c<8%组（48例）和HbA1c≥8%组（58例）。比较两组患者的年龄、HbA1c比例、菌斑指数、牙周探诊深度。采集入选牙位的龈下菌斑,采用聚合酶链式反应（PCR）检测6种可疑牙周致病菌数量并统计检出率;分析龈下病菌感染与HbA1c的相关性。结果： HbA1c≥8%组患者龈沟出血指数、探诊深度、福塞坦氏菌、中间普氏菌和具核梭杆菌数量和检出率均明显高于HbA1c<8%组（P均<0.05）;牙龈卟啉单胞菌、福塞坦氏菌和具核梭杆菌与患者HbA1c呈正相关（P<0.05）,中间普氏菌与HbA1c呈负相关（P<0.05）。 结论： 糖尿病患者的血糖水平与种植体龈下牙周致病菌菌群的繁殖有相关性。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Treatment of Vomiting

CASE HISTORY A female patient, 46 years old, head of the foreign affairs department of a certain university in Beijing, paid her first visit on October 9, 2006, with the chief complaint of vomiting for one month. She got vomiting after meals in early September. Before that, she had discomfortable sensation in the stomach due to angry with others, but she didn't pay much attention. Later, it developed into vomiting after eating. After the vomiting, the discomfort would be relieved, but with slight hypodynamia. She was once diagnosed as having 'neurogenic vomiting'. Having taken some western and Chinese drugs, the above symptoms were a little bit improved, but she would have nausea upon eating and with regurgitation. Because of the fear for vomiting, she did not dare to have food intake, with body weight reduction of 6 kilos in one month.     

Qiu Baoguo''''s Experience in Treating Consumptive Syndromes after Radio-Chemotherapies

Radiotherapy and chemotherapy are the important modern medical therapies for malignant tumors,yet they can also bring about serious local and systemic toxic side reactions so to decrease the patient;'s life quality,manifested by a series of consumptive symptoms.Having engaged in the combined work of Chinese and western medicine for nearly 50 years,the research fellow Qiu Baoguo in Henan Provincial Academy of TCM has developed his unique views on the TCM study of consumptive syndromes.The author of this essay had once the fortune tO follow Dr.Qiu in clinic,and specially would like to introduce in the following Dr.Qiu's experience in treating consumptive syndromes after radio-chemotherapies for patients with malignant tumor.     

Insomnia Due to Deficiency of Both the Heart and Spleen Treated by Acupuncture-Moxibustion and Chinese Tuina

OBJECTIVE: To observe therapeutic effects of the comprehensive therapy of acupuncture-moxibustion and Chinese Tuina for treatment of insomnia due to deficiency of both the heart and spleen. METHODS: 92 cases were divided randomly into the treatment group (treated by acupuncture-moxibustion and Chinese Tuina) and the control group (treated by acupuncture-moxibustion). RESULTS: The therapeutic effect of the treatment group was obviously superior to that of the control group (the CHI2 test showed P < 0.01). CONCLUSIONS: The comprehensive therapy of acupuncture-moxibustion and Chinese Tuina can give marked therapeutic effects for treatment of insomnia due to deficiency of both the heart and spleen.     

Analysis of CK-19,PDX-1,Nestin,Ngn3 in different donor islet purity in rats

Objective: To investigate if there are the CK-19, PDX-1, Nestin, Ngn3 positive cells in the donor islets of different purity in rats. Methods: Thirty male adult SD rats were randomly divided into 3 groups. Islets were isolated using digestion by ductal injection of collagenase. Group Ⅰ (n=10): Separating cell preparations were not purified, Group Ⅱ(n=10): Islet sediment was purified with 25% Ficoll400 ,Group Ⅲ (n=10): Islet sediment was purified with 25% and 11% Ficoll-400. The levels of protein of CK-19, PDX-1, Nestin and Ngn3 were detected by immunohistochemistry and the mRNA of CK-19, PDX-1, Nestin, Ngn3 was amplified by RT-PCR. Results: After two different purification methods applied, three islet preparations of different purities were obtained. The difference of islet purity was significant among various groups (P＜0.05). Compared with group Ⅱ and group Ⅲ,the protein and mRNA of CK-19, PDX-1, Nestin,Ngn3 were both higher in group Ⅰ; group Ⅲ was poorly expressed. Conclusions: The three different islet purity donor islet have different CK-19, PDX-1, Nestin, Ngn3 positive cells within them, indicating that there are some islet stem cells in the purified donor islet.     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.