宫颈上皮内瘤变中人乳头瘤病毒基因分型及感染状况研究

目的:探讨宫颈上皮内瘤变中人乳头瘤病毒(HPV)基因分型及感染状况,为临床宫颈癌的防治提供理论依据。方法:临床病理诊断为宫颈上皮内瘤变(CIN)石蜡包埋的组织标本150例为研究对象。采用DNA芯片技术,对其HPV感染状况进行回顾性分析。结果:1HPV阳性筛查率在20~39岁年龄段处于较高水平,其中20~29岁年龄段阳性率最高,为26.92%,宫颈上皮内瘤变好发年龄组为30~59岁之间。2CIN I常见的HPV基因型为:16、58;CIN II为16、31;CIN III为16、52。结论:HPV易感与CIN高发为不同年龄组,在不同级别CIN中HPV基因亚型不同。     

248例宫颈上皮内瘤变患者人乳头瘤病毒感染状况分析

目的研究和分析宫颈上皮内瘤变患者人乳头瘤病毒(HPV)感染状况。方法选取2016年至2017年在该院就诊以及治疗的248例病理诊断为宫颈组织病变患者采用聚合酶链式反应(PCR)扩增及导流杂交基因芯片技术对患者宫颈脱落细胞进行HPV基因分型检测,观察不同年龄阶段及不同级别宫颈上皮内瘤变患者HPV的感染状况。结果 248份样本中,共234份检测出HPV阳性,阳性率为94.35%;其中单一基因型139份,占58.90%,多基因型混合感染78份,占33.05%,其他型占8.05%;根据宫颈上皮内瘤变(CIN)患者的年龄将其分组,结果显示31～40岁组HPV检出阳性率最高为33.47%,高于20～30岁组及41～50岁组,分别为22.46%及25.42%,明显高于51～60岁组的11.86%;各年龄组之间差异有统计学意义(χ~2=32.017, P 0.01)。CIN I、CIN II、宫颈原位癌组均以高危型HPV16感染率最高。HPV感染检出率在宫颈息肉和子宫平滑肌瘤组及CIN I、CIN II、宫颈原位癌组分别为0、75.00%、93.62%和79.07%,随着宫颈病变级别的增加,HPV的总阳性率逐渐上升(P 0.05)。结论正常宫颈及不同级别的CIN患者HPV感染情况不同,不同亚型HPV在不同程度的CIN患者中的感染率不同,不同年龄对HPV的感染率也有所不同。     

人乳头瘤病毒基因型对宫颈上皮内瘤变严重程度及LEEP术预后的影响

目的：探究HPV基因型对患者发生宫颈上皮内瘤变的严重程度和对患者行LEEP术后预后的影响。方法收集2007-2013年我院223例有宫颈上皮内瘤变(CIN)并行LEEP环切术的患者进行研究,患者按CIN严重程度分为CIN1~2和CIN3(含原位癌CIS)两组;HPV基因型按：(1)低危;(2)中危(;3)高危HPV16;(4)高危HPV18;(5)未归类;共分为五组;术后患者按手术切缘是否存在残留病灶分为手术切缘阳性组和阴性组。采用核酸杂交技术检测患者所感染的HPV基因型,比较组间HPV型别的分布差异。结果 CIN3是所选患者中最常见的CIN类型(占68.6%),HPV16是CIN患者中最常见的基因型(占26.9%)。与CIN1~2组的患者比较,中危、16、18型的HPV在CIN3组患者中所占比例更高(P<0.05)。HPV18型感染组手术切缘阳性发生率最高(占60%)(P<0.05)。结论 HPV基因型别与患者的CIN严重程度密切相关,开展HPV筛查可为宫颈病变提供早期诊断和预后判断;HPV基因型所致患者手术切缘的阳性率不同,其中HPV18型患者手术切缘阳性率最高,因此感染HPV18型的CIN患者在术后更需仔细追踪。     

高危型HPV 16/18感染与宫颈上皮内瘤变的相关性

目的探讨高危型人乳头状瘤病毒（HPV）16／18感染与宫颈上皮内瘤（CIN）的关系。方法用膜式液基薄层细胞学（TCT）技术筛查与病理学诊断相结合，对宫颈炎和宫颈上皮内病变一级及其以上（≥CIN1）病例各200例进行HPV16／18型荧光定量PCR检测。结果宫颈病变≥CIN1组阳性病例69例，阳性率34．5％；宫颈炎对照组阳性．病例16例，阳性率8．0％，两组比较差异有统计学意义（P〈0．01）。结论高危型HPV16／18感染与宫颈上皮内瘤变密切相关。     

重庆市农村妇女高危型HPV感染状况分析

目的 分析重庆市农村妇女高危型人乳头瘤病毒(HPV)感染情况及其与宫颈病变的关系.方法 2016年1-4月选取重庆市万州区、永川区、巴南区、潼南区共20000例农村妇女,以HPV基因分型检测对其进行宫颈癌的初筛.对HPV16、18型(+)者或其余12种HPV高危型(+)阴道镜检查异常者进行阴道镜定点活检.结果 重庆市农村妇女高危型HPV感染阳性率为9.66%,不同年龄组的高危型HPV感染阳性率及不同高危型别HPV感染阳性率均有差异.高危型HPV感染阳性者中,宫颈上皮内瘤变(CIN)1发生率为2.33%,CIN2、CIN3及AIS发生率为1.35%,宫颈癌发生率为0.31%,不同年龄组各级别宫颈病变发生率差异无统计学意义.不同高危型别HPV感染者中,HPV16阳性者各级别宫颈病变发生率均最高,而其余12型HPV高危型别阳性者各级别宫颈病变发生率均最低,且差异有统计学意义.结论 HPV基因分型检测可提高宫颈癌的筛查效率,更适合在大规模人群中普查.     

宫颈病变人乳头状瘤病毒分子检测及分型的意义

目的 探讨宫颈癌前病变患者进行人乳头状瘤病毒(HPV)DNA检测及基因分型的意义.方法 采集2153例宫颈中心就诊患者的宫颈脱落细胞,采用杂交捕获技术对HPV DNA进行检测.采用凯普导流杂交HPV分型技术检测342例宫颈上皮内瘤变(CIN)患者的HPV亚型.结果 ①2153例受检者中701例HPV DNA检测阳性.阳性率达32.6%;其中以宫颈癌患者感染率最高,达93.9%,其次为宫颈上皮内瘤变,达54.6%.②高危型HPV感染好发于30岁以上,40～59岁是高危型HPV最高发的年龄阶段.③随着宫颈病变的严重程度,高危型HPV DNA病毒载量有上升趋势.④宫颈上皮内瘤变患者高危型HPV主要的感染类型为16、52和58亚型.低危型主要为11、6和42亚型.⑤CIN I主要感染亚型为16、52和33亚型,CINⅡ主要亚型为16、58和18亚型.CINⅢ主要亚型为16、52和58亚型.⑥高危型HPV感染者多重感染率为23.O%,其中双重感染18.9%.三重及以上感染4.1%.CIN I、CINlI和CIN III多重感染发生率未发现明显增高趋势.结论 高危型HPV DNA检测用于宫颈癌前病变筛查极有价值.HPVl6仍然是最应关注的高危亚型.     

不同亚型人乳头瘤病毒在宫颈疾病中的分布特点

目的:分析不同基因型的人乳头瘤病毒(HPV)在不同级别宫颈疾病中的分布特点,探讨其与宫颈癌的相关性?方法:对2012年1~12月在南京市妇幼保健院门诊拟诊有宫颈疾病(TCTASCUS和/或HPV分型异常)的665例患者进行阴道镜及组织病理检测,对结果进行统计分析?结果:共检测出14种HPV亚型,HPV阳性率随着宫颈上皮内瘤变(CIN)级别升高而增加;CIN中以高危型HPV感染为主,尤其以HPV16?58感染率最高?结论:对HPV进行分型检测,可以预测CIN发展?     

宫颈上皮内瘤变136例临床分析

目的探讨宫颈上皮内瘤变(CIN)与人乳头瘤病毒(HPV)感染的关系及其临床处理方法.方法用新柏氏液基超薄细胞检测(TCT)的1 436例妇科患者宫颈分泌标本,探讨CIN的阳性率、CIN与HPV感染的关系及处理方法.结果受检者中CIN阳性136例,阳性率为9.5%;HPV阳性74例,阳性率5.2%,CIN分级与HPV感染呈正相关.结论宫颈上皮内瘤变及其级别与HPV感染之间有重要关系,TCT超薄细胞学检测对早期发现、预防和治疗宫颈癌意义重大.     

泸州地区女性人乳头状瘤病毒感染与宫颈病变关系研究

目的：应用膜杂交多重检测技术进行人乳头状瘤病毒（HPV）基因分型诊断,结合宫颈活体组织检查探讨泸州地区女性HPV感染与宫颈病变的关系。方法：采用膜杂交多重检测技术对泸州地区1142例门诊就医和体检女性进行HPV感染定性检测和型别诊断,结果为阳性者建议做宫颈活体组织检查。结果：在1142例标本中,共检出HPV阳性标本301例,阳性率为26.36%,共检出18种基因型,高危型以HPV16、58感染为主,低危型以HPV6、11感染为主。接受宫颈活体组织检查的225例HPV阳性女性中,检出宫颈上皮内瘤变及浸润癌共112例（CINⅠ28例,CINⅡ25例,CINⅢ46例,浸润癌13例）。结论：HPV分型检测联合组织学检查能及时有效地筛查宫颈疾病,为宫颈癌的防治提供可靠的科学依据。     

高危型HPV-DNA染色体整合状态与宫颈上皮内瘤变及宫颈癌关系研究

目的研究高危型HPV—DNA染色体整合状态与宫颈上皮内瘤变及宫颈癌之间的关系。方法应用原位杂交和免疫组化方法检测95例宫颈上皮内瘤变（CINI、CINII、CINm级分别21,18,16例）及宫颈癌组织中HPV感染情况,观察阳性杂交病例中HPV-DNA染色体整合状态与宫颈上皮内瘤变及宫颈癌关系。结果免疫组化检测CINⅠ、CINⅡ、CINⅢ级阳性率分别为14％,16．6％,37．5％,宫颈鳞癌阳性率46．6％（P〈0．05）;原位杂交检测CINⅠ、CINⅡ、CINⅢ级阳性率分别为19％,27．7％,37．5％,宫颈鳞癌阳性率70％（P〈0．05）;在CINⅠ、CINⅡ级中HPV—DNA以染色体外游离状态存在,在CINⅢ级及宫颈鳞癌中出现HPV—DNA整合状态。结论HPV．DNA染色体整合状态与宫颈上皮内瘤变及宫颈癌有关,在一定程度上HPV—DNA染色体整合状态对宫颈上皮内瘤变的恶性转归具有提示意义。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.