马鞭草化学成分的分离与鉴定（I）

目的 探索马鞭草兴奋子宫的有效成分。方法 马鞭草甲醇提取物，经反复硅胶柱层析分离，理化性质和光谱分析鉴定其结构。结果 分离获得６个化合物单体，鉴定了其中３个化合物分别为十六酸（Ｉ）、乌索酸（Ⅱ）和β－谷甾醇（Ⅲ），其它３个化合物的结构待鉴定。结论 其中化合物Ⅰ、Ⅱ为首次从中国产马鞭草中获得。     

马鞭草抗乙肝有效部位化学成分研究

目的研究马鞭草（Verbena officinalis L．）抗乙肝有效部位的化学成分。方法采用柱层析和薄层层析分离马鞭草有效部位中的化学成分;用IR、ESI—MS、1^H—NMR、13^C-NMR等波谱技术鉴定结构。结果分离并鉴定了6个化合物,分别为山柰酚（1）、槲皮素（2）、杨梅素（3）、熊果酸（4）、马鞭草苷（5）和杨梅苷（6）。结论首次报道马鞭草抗乙肝有效部位的化学成分;化合物3、6为首次从该植物中分离得到。     

三花莸的化学成分研究

三花莸Caryopteris terniflora Maxim．为马鞭草科莸属植物，又名野荆芥、大风寒草等。主要分布于河北、山西、陕西、甘肃等地。全草药用，具有解表散寒、宣肺之效。治疗外感头痛、咳嗽、外障目翳、烫伤等症。其化学成分未见报道。为探讨其有效成分，对其化学成分进行了系统研究，从其正丁醇部分分离并鉴定了4个化合物，这些化合物分别为两个脑苷脂类化合物：大豆脑苷Ⅱ(soya-cerebroside Ⅱ，     

马鞭草苷现代药学与生物活性研究进展

马鞭草苷是药用植物马鞭草中的重要活性成分。马鞭草分布广泛,资源丰富,临床应用价值较高。马鞭草苷具有很好的研究开发前景。本文从马鞭草苷的含量测定、分离提取、药动学研究和生物活性等几个方面进行阐述,为马鞭草苷更深入的开发利用提供科学的理论依据。     

莴笋花化学成分的研究

目的：研究莴笋花Ｃostus lacerus的化学成分，方法采用硅胶柱层析分离,Sephadex LH-20柱纯化，分得１０个化合物：通过波谱技术和化学降解进行８个化合物的结构鉴定。结果它们分别为：二十八烷（Ⅰ），β－谷甾醇（Ⅱ），薯蓣皂苷元（Ⅲ），胡萝卜苷（Ⅳ），薯次苷Ｂ（Ⅴ），薯蓣次苷Ａ（Ⅵ），薯蓣皂甙（Ⅶ），纤维薯蓣皂苷（Ⅷ）。结论：化合物Ⅰ，Ⅱ，Ⅲ和Ⅴ为首次从该植物中分得。     

对马鞭草中环烯醚萜苷类生物合成路径的探讨

目的：探讨马鞭草中环烯醚萜苷类的生物合成路径。方法：分析马鞭草中环烯醚萜苷类化合物结构并与其生物合成路径对照。结果：新化合物3，4－二氢马鞭草苷的结构不符合这一生物合成路径。结论：马鞭草中环烯醚萜苷类的生物合成可能存在其它路径。     

卷叶贝母化学成分研究

目的对百合科植物卷叶贝母母的干燥鳞茎进行化学成分研究。方法通过提取和层析进行成分分离。运用化学和现代光谱手段进行结构鉴定，结果从中分得３个化合物，分别鉴定为（２２Ｒ，２５Ｓ）－ｓｏｌａｎｉｄａｎｅ－３β－醇（Ⅰ）、胸苷（Ⅱ）和腺苷（Ⅲ）。结论化合物Ⅰ为首次从贝母属植物中分得，腺苷具有抑制由ＰＡＦ诱导的血小板集活性。     

细梗紫菊中的化学成分

目的 对细梗紫菊Notoseris gracilipes全草的化学成分进行分离纯化、结构鉴定和抗菌活性测试。方法 以甲醇室温提取，采用各种色谱技术进行分离纯化。根据NMR等波谱数据鉴定结构。按常规方法进行抗菌活性的定性测定。结果 从细梗紫菊全草的甲醇提取物中分离鉴定出12个化合物，其中8个为倍半萜内酯：jacquilenin(Ⅰ），scorzoside(Ⅱ），ixerisosideD(Ⅲ）,austricin(Ⅳ）,notoserolideA(Ⅴ），crepidiasideB(Ⅶ）和lactusideB(Ⅷ）。另外4个化合物分别鉴定为6，7-二羟基香豆素（Ⅸ），野莴苣苷（Ⅹ），木犀草素-7-葡萄糖苷（Ⅺ）和胡萝卜苷（Ⅻ)。倍半萜内酯的抗菌实验表明化合物Ⅰ和Ⅱ对蜡状芽孢杆菌（Bacillu cereus AS.1.1688)的生长有抑制作用。结论 这些化合物均为首次从该植物中分离得到。     

滇白珠化学成分的研究

从滇白珠Ｒｅｈｄ。根的氯仿提取物中分离得到１１个化合物，其中７个化合物经理化 波谱分析分别鉴定为东莨菪素（Ⅰ），棕榈酸（Ⅱ），胡萝卜苷（Ⅲ）（＋）－ｌｙｏｎｉｒｅｓｉｎｏｌ（Ⅳ）－甲氧基异落叶松树脂醇（Ⅴ），３，４，５－三甲氧基苯甲酸（Ⅵ）和乙酰丁香酸（Ⅶ）。化合物Ⅴ为一新天然产物，其它６个化合物为该属植物中首次报道。     

兵豆化学成分的研究

目的：研究云南产的兵豆种子中的化学成分，为资源开发利用提供依据。方法：采用溶剂法和层析法分离、纯化兵豆化学成分，并通过理化鉴定与波谱分析，测定所分离出化合物的分子结构。结果与结论：从兵豆果实的乙醇提取物中分离到８个化合物，分别鉴定为β－谷甾醇（Ｉ）、β－谷甾醇棕榈酸酯（Ⅱ）、胡萝卜素苷（Ⅲ）、棕榈酸（Ⅳ）、９，１０－二羟基硬脂酸（Ⅴ）、乙基－α－Ｄ－吡喃葡糖苷（Ⅵ）、３－Ｏ－甲基－Ｄ－手性肌醇（Ⅶ     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.