采用低离子介质制备液体型凝血酶试剂稳定性观察

目的采用自备低离子缓冲液稀释凝血酶,探讨制备稳定的凝血酶时间（TT）测定试剂。方法将国产凝血酶冻干粉用自备低离子缓冲液溶解、稀释并标化。分装后置-20℃保存。观察低离子液体型TT试剂在-20℃冷存的稳定性和解冻后在全自动凝血分析仪工作环境下（15℃）的稳定性。并与进口冻干试剂进行平行试验。结果低离子液体型TT试剂在-20℃能保存6个月以上,解冻后在15℃环境中能稳定8 h。结论用低离子缓冲液和国产凝血酶制备的标准化TT试剂在低温下能长期保存,在全自动凝血分析仪工作环境下的稳定性和重复性符合TT实验要求。     

凝血酶试剂仪器工作环境稳定性分析

目的探讨凝血酶试剂在仪器工作环境中(15℃)的放置时间长短对检测结果的影响.方法将干粉凝血酶试剂溶解后放置于检测仪器试剂位置,放置不同时间后测定病人标本凝血酶时间.结果凝血酶试剂溶解后在15℃环境中放置10小时后对测定结果影响有显著差异(p<0.01).结论凝血酶试剂溶解后在仪器工作环境能稳定8h,若要较长时间保存应冷冻保存(-20℃).     

凝血酶对U937细胞uPAR mRNA表达的影响

目的 研究凝血酶通过凝血酶受体（ＴＲ）对细胞ｕＰＡＲ ｍＲＮＡ表达的影响。方法 在凝血酶、灭活凝血酶、和抗ＴＲ小肽多抗等作用下,以ＲＴ－ＰＣＲ法测定培养Ｕ９３７细胞ｕＰＡＲ ｍＲＮＡ的表达量。结果 凝血酶增强ｕＰＡＲ ｍＲＮＡ表达呈现凝血酶剂艇时间的依赖性。其作用机制与凝血酶和细胞膜上ＴＲ间的结合、以及凝血酶的蛋白水解活性有关。高浓度凝血酶或中等浓度凝血酶在延长作用时间长,对Ｕ９３７细胞ｕＰＡＲｍ     

凝血酶对U937细胞uPAR mRNA表达的影响

目的"研究凝血酶通过凝血酶受体(TR)对细胞 uPAR mRNA 表达的影响. 方法"在凝血酶、灭活凝血酶、和抗TR小肽多抗等作用下,以RT-PCR法测定培养U937细胞uPAR mRNA的表达量. 结果"凝血酶增强uPAR mRNA表达呈现凝血酶剂量和作用时间的依赖性.其作用机制与凝血酶和细胞膜上TR间的结合、以及凝血酶的蛋白水解活性有关.高浓度凝血酶或中等浓度凝血酶在延长作用时间时,对U937细胞uPAR mRNA表达有明显抑制效应. 结论"凝血酶对U937细胞uPAR mRNA表达的影响,除通过活化TR外可能还存在其它的途径,其确切机制尚需进一步研究.     

不同溶剂溶解凝血酶对凝血酶促凝活性的影响

目的比较凝血酶在不同溶剂中的凝血活性。方法用9种不同的溶剂稀释凝血酶并分为两种浓度、一种为低浓度,另一种为高浓度,低浓度凝血酶和高浓度凝血酶分别在CA1500全自动凝血仪上对同一枸橼酸抗凝血浆进行凝血酶时间（thrombin time,TT）和纤维蛋白原（fibrinogen,Fbg）测定。结果 TT和Fbg均提示：凝血酶在水溶液中的促凝活性最强。0.9%NS和5%葡萄糖溶液具有抑制凝血酶凝血活性的作用,且浓度越高抑制作用越强,以5%GNS对凝血酶的凝血活性抑制作用最强。结论凝血酶作为外用止血时,采用日常生活中极为常见的蒸馏水及纯净水稀释,可能会提高其止血效果     

凝血酶对大鼠离体胸主动脉和肺动脉的收缩作用及其可能机制

目的观察凝血酶对离体大鼠胸主动脉与肺动脉血管张力的影响,并初步探讨其可能的作用机制。方法采用Powerlab生物信号采集放大系统记录离体大鼠肺动脉与胸主动脉的张力信号,利用RT-PCR技术检测血管壁凝血酶受体的表达。结果凝血酶对离体大鼠肺动脉及胸主动脉具有明显的收缩作用,且该收缩作用与凝血酶受体-1激动剂的作用相似,不依赖于细胞外钙离子的存在。大鼠肺动脉及胸主动脉血管壁中均有凝血酶受体-1的表达。结论凝血酶对大鼠离体血管具有收缩作用并且可能是通过凝血酶受体-1而起作用,该作用不依赖于细胞外钙离子的存在。     

浅谈止血药凝血酶的研究进展

凝血酶是机体凝血系统中的天然成分,是一种生成于损伤处血管内皮细胞的多功能蛋白酶,它是参与凝血过程各个环节反应中的关键酶.本文介绍了凝血酶的结构、性质、在体内凝血机制中的作用及临床应用,并分析了其作为一种重要的外伤止血药的生产现状和前景.     

一种检测凝血酶、类凝血酶的新方法—纤维蛋白原平板法

目的：创建一种定量检测凝血酶（Thrombin）、类凝血酶（Thrombin-likeenzymes）活性的新方法—纤维蛋白原平板法。方法：采用纤维蛋白原作为底物，用琼脂糖与纤维蛋白原制作成纤维蛋白原平板，以凝血酶、类凝血酶标准品分别在该平板上的扩散反应，制作相应的标准曲线及回归曲线，并对凝血酶、类凝血酶活性作定量测定。结果：本法制作的凝血酶、类凝血酶检测标准曲线具有良好的线性关系（r=0.9994、r=0.9997）。结论：纤维蛋白原平板法可用于凝血酶、类凝血酶定量测定。具有操作简便、直观、稳定、敏感度高，不受检品浊度或颜色干扰且成本低廉等特点。     

凝血酶对食管癌细胞EC109细胞迁移的影响

目的：探讨凝血酶对食管癌细胞EC109细胞迁移的影响。方法：EC109分别接种于96孔培养板各孔,贴壁后划痕,分别加入不同浓度的凝血酶,作用时间12、24、48、72h。同时设置水蛭素组、凝血酶＋水蛭素组及无药组。拍照并测量计算细胞迁移距离。结果：凝血酶组各浓度在不同作用时间的迁移距离与水蛭素组、凝血酶＋水蛭素组和无药组比有统计学意义（P〈0.05）,水蛭素组、凝血酶＋水蛭素组与无药组比无统计学意义,凝血酶组各浓度间比无统计学意义。结论：凝血酶可增强EC109细胞迁移能力。     

凝血酶受体在多发性骨髓瘤细胞XG-1增殖和抗凋亡作用中的机制

目的研究凝血酶及其受体在促进多发性骨髓瘤细胞增殖和抗凋亡作用中的机制。方法采用免疫荧光标记、流式细胞仪检测、细胞计数和免疫印迹等方法,对多发性骨髓瘤细胞株XG-1细胞表达的凝血酶受体、凝血酶对XG-1的增殖与抗凋亡作用及细胞内JAK2-STAT3分子磷酸化进行分析。结果凝血酶可以通过激活XG-1表面的凝血酶受体,继而激活JAK2-STAT3途径的磷酸化,促进骨髓瘤细胞的增殖与抗凋亡作用,且该作用与gp130介导的信号转导作用无关。结论凝血酶及其受体介导的信号转导通路是多发性骨髓瘤维持增殖和抗凋亡作用的因素之一。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…