Apo H基因多态性与冠心病及血脂代谢的关系

目的探讨载脂蛋白H（ApoH）外显子3、8基因多态性与冠心病（CHD）及血脂代谢的关系。方法采用聚合酶链式反应结合限制性片段长度多态分析方法，分析了100例健康人及110例冠心病病人的ApoH外显子3、8基因型，并对血脂进行了测定。结果冠心病组ApoH外显子3GG、GA＋AA基因型频率及G、A等位基因频率与对照组比较差异无显著性（X^2=1．56～2．15，P〉0．05）。冠心病组ApoH外显子8GC基因型频率及C等位基因频率显著增高（X^2=3．91、8．44，P〈0．05）。冠心病组ApoH外显子3GG、GA＋AA基因型病人高密度脂蛋白胆固醇（HDL）显著高于对照组（t’=8．30，t=4．06，P〈0．05）；冠心病组及对照组内基因型间的血脂水平比较无明显差异。冠心病组外显子8GG、GC基因型病人LDL水平也显著高于对照组（t’=7．97、8．55，P〈0．05）；冠心病组GC基因型病人的三酰甘油（TG）水平显著高于GG型及对照组的各基因型（t’=4．02～4．71，P〈0．05）。结论ApoH外显子3基因多态性与冠心病及血脂代谢无相关性；ApoH外显子8GC基因型及C等位基因频率与冠心病发病有关，ApoH外显子8基因多态性与TG代谢有关。     

Apo H基因多态性与冠心病及血脂代谢的关系

目的 探讨载脂蛋白H(Apo H)外显子3、8基因多态性与冠心病(CHD)及血脂代谢的关系.方法 采用聚合酶链式反应结合限制性片段长度多态分析方法,分析了100例健康人及110例冠心病病人的ApoH外显子3,8基因型,并对血脂进行了测定.结果 冠心病组Apo H 外显子3 GG、GA+AA、G等位基因频率、A等位基因频率与对照组比较无差异(X2=1.56～2.15,P>0.05);与对照组比较冠心病组Apo H外显子8 GC基因型频率及C等位基因频率显著增高(x2=3.91～8.44,P<0.05).冠心病组Apo H 外显子3GG、GA+AA基因型LDL高于对照组(q=3.89,P<0.05),冠心病组及对照组各基因型间的血脂水平无差别(q=3.37,P >0.05);冠心病组外显子8 GG、GC基因型LDL也显著高于对照组(q=4.26,P<0.05),冠心病组GC基因型的三酰甘油(TG)显著高于GG型及对照组的各基因型(q=4.09,P<0.05).结论 APO H外显子3基因多态性与冠心病及血脂代谢无相关性;APO H外显子8 GC基因型及C等位基因与冠心病有关,APO H外显子8基因多态性与TG有关.     

急性白血病病人胞苷脱氨酶基因多态性对阿糖胞苷疗效的影响

目的 探讨急性白血病病人CDA基因第1号外显子基因多态分布以及追踪随访不同基因型患者的疗效与转归。方法 采用PCR扩增目的片段并直接测序采了解CDA基因G208A位点基因多态。结果 发现CDA基因第1号外显子存在G208A多态，有GG、GA和从3种基因型，其G等位基因频率为95．14％，而A等位基因频率为4．86％。同时发现未缓解比率在GG与GA和从两组之间无统计学差异（X^2=0．96，P〉0．05），但其复发率在两组之间存在差异（X^2=5．04，P=0．02〈0．05），GG基因型的患者复发率较低。结论 CDA基因第1号外显子在其研究人群中存在G208A多态，其不同的基因型对患者预后存在影响。     

蛋白Z启动子A-13G基因多态性与冠心病相关研究

目的 探讨蛋白Z启动子A-13G基因多态性在浙江地区汉族人群中的发生率及其与冠状动脉粥样硬化性心脏病（简称冠心病）的关系。方法 对冠心病组（至少1支冠脉直径狭窄≥50．00％）157例和对照组129例，采用聚合酶链反应和限制性内切酶片段长度多态性方法结合基因测序技术，检测蛋白Z启动子A-13G基因多态性。结果首次发现浙江地区汉族人群存在蛋白Z启动子A-13G基因多态性，野生型（AA型）、GA型和GG型分别占16．78％、53．85％和29．37％；A、G等位基因频率分别为43．71％和56．29％；冠心病组与对照组间基因型和等位基因频率分布差异均无显著性意义（X^2=1．17、0．32，均P〉0．05）；冠脉单支、双支和三支病变患者间基因多态性分布差异亦无显著性意义（X^2=1．17，P〉0．05），而蛋白Z启动子A-13G基因型在急性冠脉综合征和非急性冠脉综合征患者间的分布差异有显著性意义（P〈0．05），AA型冠心病患者患急性冠脉综合征的危险是GG型的3．42倍。结论 浙江地区汉族人群存在蛋白Z启动子A-13G基因多态性，蛋白Z启动子A-13G位点A→G突变可能对急性冠脉事件发生有保护作用。[编者按]     

布依族XRCC1基因单核苷酸多态性及其组合型分析

目的 分析布依族人群DNA修复基因XRCC1 C26304T、G27466A与G28152A单核苷酸多态性及其等位基因频率与组合分布特征。方法 获取自然人群个体布依族192例血白细胞基因组DNA，利用PCR扩增限制性酶切法（PCR—RFLP）检测XRCC1 C26304T、G27466A与G28152ASNPs。结果 XRCC1 C26304TSNPs基因型分布CC、CT、TT分别为64．6％、28．6％、6．8％，C、T等位基因频率分别为78．7％、21．3％。XRCC1G27466ASNPs基因型分布GG、GA、从分别为85．9％、12．5％、1．6％，G、A等位基因频率分别为91．7％、8．3％。XRCC1G28152ASNPs基因型分布GG、GA、从分别为55．2％、37．0％、7．8％，G、A等位基因频率分别为73．7％、26．3％。本研究发现XRCC1基因C26304T和G28152A两位点的SNPs组合基因型频率较高的为CC＋GG60例（31．2％）；C26304T和G27466A两位点的SNPs组合基因型频率较高的为CC＋GG98例（51．0％）；G28152A和G27466A两位点的SNPs组合基因型频率较高的为GG＋GG88例（45．8％）。结论 本研究揭示了布依族人群XRCC1基因3位点的单核苷酸多态性、基因型及其组合分布特征，为进一步研究该基因SNPs与其生理功能和疾病的关系提供基础资料。     

CTLA-4基因外显子1的49位点A／G多态性与吉兰-巴林综合征的关联研究

目的探讨细胞毒性T淋巴细胞抗原4（CTLA-4）基因外显子1的49位点A／G多态性与吉兰-巴林综合征（GBS）易患率的相关性。方法采用多聚酶链反应限制性片段长度多态性（PCR—RVt,P）技术分析10例吉兰-巴林综舍征患者及20例正常人CTLA-4基因外显子1的49位点A／G多态性。结果CTLA-4基因的3种基因型在GBS患者组中分布情况为：从型30．0％；AG型50．0％；GG型20．0％。在对照组中分布情况为：从型35．0％；AG型50．0％；GG型15．0％（P〉0．05）。CTLA-4／G49等位基因频率在GBS患者组为45．0％，在对照组为40％（P〉0．05）。结论CTLA-4基因外显子1的49位点A／G多态性与GBS无显著性关联。     

RGS2基因多态性与淮安地区汉族重度子痫前期易感性的相关性

目的：探讨RGS2基因rs4606位点单核苷酸多态性与淮安地区汉族重症子痫前期发病风险的相关性。方法住院分娩的汉族重度子痫前期患者284例为病例组（ PE组）,同期汉族正常妊娠孕妇316例作为对照组,采用基质辅助激光解吸附电离飞行时间质谱对RGS2基因rs4606位点进行基因分型。结果 PE组RGS2基因rs4606位点CC、GC和GG基因型频率分别为28．5％、49．3％和22．2％,等位基因频率C、G分别为53．2％和46．8％;对照组CC、GC和 GG 基因型分别为24．4％、51．6％和24．1％,等位基因频率 C、G 分别为50．2％和49．8％。 rs4606位点的基因型和等位基因频率在2组间的差异无统计学意义（P＝0．051）。结论 RGS2基因rs4606位点多态性与淮安地区汉族重度子痫前期发病风险无关。     

宁夏回、汉族群体IL-6启动子区基因多态性研究

目的探讨白细胞介素6（intedeukin6,IL-6）基因多态性在宁夏回、汉族人群中的分布特征。方法应用聚合酶链反应一限制性片段长度多态性（PCR-RFLP）方法,对210例回族和308例汉族人群白细胞介素6基因启动子区三个多态性位点（-572G／C、-597G／A、-174G／C）进行检测。结果宁夏回族群体肛-6基因三个SNP各基因型频率及等位基因频率分别为,-572G／C（CC：55．71％;CG：34．76％;GG：9．52％;C：73．10％;G：26．90％）;-597G／A（GGl94．29％;GAl5．71％;AA：0％;G：97．14％;A：2．86）;-174G／C（GG：96．67％;GC：3．23％;CC：O％;G：98．33％;C：1．67％）。宁夏汉族群体IL-6基因三个SNP各基因型频率及等位基因频率分别为,-572（CC：52．92％;CG：35．71％;GG：11．36％;C：70．78％;G：29．22％）;-597（GG：92．86％;GA：7．14％;AA：0％;G：96．13％;A：3．57）;-174（GG：97．08％;GC：2．92％;CC：0％;G：98．54％;C：1．46％）。两组间差异均无统计学意义（P〉0．05）;按性别分组,三个SNP位点的基因型频率及等位基因频率差异亦无统计学意义（P〉0．05）。结论IL-6基因-572、-597、-174三个多态性位点在宁夏回、汉族人群及不同性别间差异无统计学意义。     

IL-13基因＋2044G→A多态性与儿童支气管哮喘关联性研究

目的：探讨IL-13基因＋2044G→A多态性与潍坊地区汉族人群儿童支气管哮喘发病的关系。方法采用PCR-RFLP方法,对90例支气管哮喘患儿（哮喘组）和82例健康儿童（对照组） IL-13基因＋2044G→A多态性进行检测,计算基因型和等位基因频率,采用χ2检验进行组间比较。结果两组人群IL-13基因＋2044G→A多态性分布具有显著差异,GG,GA,AA基因型频率哮喘组分别为37．8％,54．4％和7．8％,对照组分别为68．3％,28．0％,3．7％（χ2＝16．0,P＜0．01）;G等位基因和A等位基因频率哮喘组为65．0％和35．0％,对照组为82．3％和17．7％（χ2＝13．14,P＜0．01）;哮喘组AA基因型和A等位基因频率明显高于对照组。与GG基因型和G等位基因比较,携带AA基因型和A等位基因的儿童发生支气管哮喘的相对风险明显增加,OR值分别为3．84（95％CI：3．83～14．70）（P＜0．05）和2．51（95％CI：1．91～6．60）（P＜0．01）。结论 IL-13基因＋2044G→A多态性与儿童支气管哮喘的发病具有关联性,A等位基因可能是易感基因。     

胆固醇24S-羟化酶和三磷酸腺苷结合盒转运子A1基因多态性与阿尔茨海默病的相关性分析

目的探讨胆固醇24S-羟化酶（CW46）基因第二内含子A→G多态性位点和三磷酸腺苷结合盒转运子A1（ABCA1）基因第六外显子G→A（R219K）多态性位点与散发性阿尔茨海默病（SAD）易感性的关系。方法采用病例-对照研究方法,利用聚合酶链反应-限制性片段长度多态性（PCR-RVLV）技术对168例SAD患者和215名正常对照的CYP46和ABCA1基因多态性进行检测,比较不同基因型与阿尔茨海默病（AD）发病风险之间的关系。结果CYP46基因第二内含子A→G多态性等位基因频率和基因型分布在SAD组和正常对照组差异无统计学意义。ABCA1基因第六外显子G→A多态性的基因型分布在两组之间差异有统计学意义（x^2=8．230,P=0．016）,AD组携带A等位基因（G／A＋A／A基因型）的频率明显低于对照组（61．3％vs73．5％,x^2=6．444,P=0．011）。Logistic回归分析表明携带A等位基因者（G／A＋A／A基因型）比携带GG基因型者AD发病风险低43％（校正后OR=0．57,95％CI=0．36～0．91,P=0．019）,而从纯合子比携带GG基因型者AD发病风险低60％（校正后OR=O．40,95％CI=0．21～0．77,P=0．006）。结论CYP46第二内含子A→G多态性可能与AD发病无关;而ABCA1基因第六外显子G→A多态性与SAD相关,携带A等位基因或从基因型对SAD发病可能有一定的保护作用。     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Dr.Zhang Ren''''s Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

Luo Lingjie''''s Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.     

Survey and practice of reporting quality of randomized controlled clinical trials on traditional Chinese medicine

Evidence obtained from randomized controlled trials （RCTs） has been generally accepted as the gold standard in the evaluation of clinical effectiveness. Readers need to understand the trial design, implementation, results, analysis and interpretation, so as to fully Jnderstand the results of RCTs. Thus, the investigators of RCTs have to report these items in a complete, accurate and clear manner. Since 1998, we have conducted several evaluations on the reporting quality of RCTs published in Chinese journals on traditional Chinese medicine （TCM） and results have shown that there is an urgent need for higher quality RCTs on TCM.     

TCM Treatment of Ankylosing Spondylitis by Tonifying the Kidney and Strengthening the Governor Vessel

Ankylosing spondylitis is a chronic and progressive disorder with inflammation mainly involving the central axis joints. It mainly affects the cervical spine and the lumbosacral area, with the pathogenesis closely related to the kidney and the Governor Vessel (GV). TCM holds that the syndrome is deficiency in origin and excess in superficiality, which is due to insufficiency of the kidney, deficiency of GV, and blocking of the channels with the invasion of exogenous evil, leading to poor circulation of qi and blood and malnutrition of the bones, muscles and joints. The TCM method of tonifying the kidney and strengthening GV to regulate circulation of qi and blood and check the arthralgia pain should be adopted, with the Kidney-Tonifying and GV Strengthening Decoction (益肾强督汤) prescribed.     

IMPROVING P-gp EXPRESSION IN HUMAN MONONUCLEAR CELLS IN VITRO TRANSFECTED BY MULTIDRUG RESISTANCE-1 mRNA

CHEMOTHERAPY playsa greatrolein the treat- ment of malignanttumors,especiallyingynecolo- gicalones.But inanticancerchemotherapy,leuko-cytopeniaisfrequentlytheprimarydose-limitingsideeffect factor.Moreover,cancersarefrequentlychemoresistantbe-causeof overexpressionof P-glycoprotein(P-gp), which isencodedby multidrugresistancegene (MDR1 ) and detectableinup to50% ofhuman cancersand renderscellsresistancetoanticancerdrugs.The safetyand potentialtherapeuticbenefitof mdr1 gene transferredto h…