芪冬宁方通过调控自噬抑制肺癌细胞生长的分子机制研究

  目的：观察健脾解毒方通过TLRs/NF-κB信号通路对5-FU致大鼠肠黏膜炎的保护作用。  方法：将60只SD大鼠随机分为空白组、模型组、健脾解毒方低、中、高剂量组(0.31 g/mL,0.62 g/mL,1.24 g/mL)、培菲康组6组,每组10只。除空白组外,每组大鼠予5-FU(50 mg/kg)腹腔注射,每日1次,连续5 d；中药治疗组在化疗的同时分别灌胃给予低、中、高剂量健脾解毒方(3.1 g/kg,6.2 g/kg,12.4 g/kg),每天1次,连续7 d；培菲康组在化疗的同时灌胃给予培菲康(151 mg/kg),每天1次,连续7 d。每日观察大鼠腹泻情况、测量大鼠体质量,7天后HE染色观察大鼠小肠组织结构,免疫组化检测小肠组织中紧密连接蛋白Occludin蛋白的表达,ELISA法检测各组大鼠血清TNF-α、IL-10表达,Westernblot法检测肠组织中MyD88、p65与TLRs含量。  结果：①实验过程中空白组大鼠体质量进行性增加,实验第3天起模型组及各给药组大鼠体质量开始下降,实验第5天起,健脾解毒方高剂量组大鼠体质量下降小于模型组(P<0.05)；第5天起,除空白组外的其他组大鼠出现腹泻,至第6天达到腹泻高峰,且第6天健脾解毒方高剂量组的腹泻发生率低于模型组(P<0.05)。②与空白组比较,模型组Occludin蛋白表达量减少(P<0.01)；与模型组比较,健脾解毒方各剂量组Occludin蛋白表达量均增加,其中以健脾解毒方高剂量组增加最显著(P<0.01)。③模型组大鼠血清TNF-α表达高于空白组(P<0.05),各治疗组的TNF-α均低于模型组,且健脾解毒方中、高剂量组的TNF-α低于健脾解毒方低剂量组(P<0.05)；模型组大鼠的IL-10低于空白组(P<0.05),健脾解毒方高剂量组、培菲康组高于模型组(P<0.05)。④模型组MyD88、p65、TLR4蛋白表达高于空白组(P<0.01),健脾解毒方中、高剂量组、培菲康组低于模型组,且健脾解毒方中、高剂量组的蛋白表达低于健脾解毒方低剂量组(P<0.01)。  结论：健脾解毒方能够抑制TLRs/NF-κB信号通路,调节炎性相关因子,减轻5-FU所致大鼠肠道黏膜炎症。     

健脾解毒方调控TLRs/NF-KB信号通路对5-FU致大鼠肠黏膜炎的保护作用

  目的：观察健脾解毒方通过TLRs/NF-κB信号通路对5-FU致大鼠肠黏膜炎的保护作用。  方法：将60只SD大鼠随机分为空白组、模型组、健脾解毒方低、中、高剂量组(0.31 g/mL,0.62 g/mL,1.24 g/mL)、培菲康组6组,每组10只。除空白组外,每组大鼠予5-FU(50 mg/kg)腹腔注射,每日1次,连续5 d；中药治疗组在化疗的同时分别灌胃给予低、中、高剂量健脾解毒方(3.1 g/kg,6.2 g/kg,12.4 g/kg),每天1次,连续7 d；培菲康组在化疗的同时灌胃给予培菲康(151 mg/kg),每天1次,连续7 d。每日观察大鼠腹泻情况、测量大鼠体质量,7天后HE染色观察大鼠小肠组织结构,免疫组化检测小肠组织中紧密连接蛋白Occludin蛋白的表达,ELISA法检测各组大鼠血清TNF-α、IL-10表达,Western blot法检测肠组织中MyD88、p65与TLRs含量。  结果：①实验过程中空白组大鼠体质量进行性增加,实验第3天起模型组及各给药组大鼠体质量开始下降,实验第5天起,健脾解毒方高剂量组大鼠体质量下降小于模型组(P<0.05)；第5天起,除空白组外的其他组大鼠出现腹泻,至第6天达到腹泻高峰,且第6天健脾解毒方高剂量组的腹泻发生率低于模型组(P<0.05)。②与空白组比较,模型组Occludin蛋白表达量减少(P<0.01)；与模型组比较,健脾解毒方各剂量组Occludin蛋白表达量均增加,其中以健脾解毒方高剂量组增加最显著(P<0.01)。③模型组大鼠血清TNF-α表达高于空白组(P<0.05),各治疗组的TNF-α均低于模型组,且健脾解毒方中、高剂量组的TNF-α低于健脾解毒方低剂量组(P<0.05)；模型组大鼠的IL-10低于空白组(P<0.05),健脾解毒方高剂量组、培菲康组高于模型组(P<0.05)。④模型组MyD88、p65、TLR4蛋白表达高于空白组(P<0.01),健脾解毒方中、高剂量组、培菲康组低于模型组,且健脾解毒方中、高剂量组的蛋白表达低于健脾解毒方低剂量组(P<0.01)。  结论：健脾解毒方能够抑制TLRs/NF-κB信号通路,调节炎性相关因子,减轻5-FU所致大鼠肠道黏膜炎症。     

健脾活血解毒方联合恩替卡韦对乙型病毒性肝炎合并非酒精性脂肪性肝病小鼠的干预作用及机制

目的 观察健脾活血解毒方联合恩替卡韦对乙型病毒性肝炎(乙肝)合并非酒精性脂肪性肝病(NAFLD)小鼠的干预作用，探讨其可能作用机制。方法 选用经重组腺相关病毒(rAAV)载体转染的小鼠15只，再经12周高脂饲料喂养构建乙肝合并NAFLD模型。将造模成功小鼠随机分为模型组、恩替卡韦组和联合中药组，每组5只。恩替卡韦组给予恩替卡韦灌胃，联合中药组给予恩替卡韦及健脾活血解毒方灌胃，模型组灌胃同等量生理盐水，连续灌胃12周。末次灌胃结束后处死小鼠，PCR检测血清HBV DNA载量，ELISA检测血清谷丙转氨酶(GPT)、谷草转氨酶(GOT)、载脂蛋白B(ApoB)、极低密度脂蛋白(VLDL)水平，HE染色及天狼星红染色观察肝脏脂肪变及纤维化情况，Western blot及Q-PCR法检测肝细胞内微粒体甘油三酯转运蛋白(MTTP)、ApoB蛋白及mRNA表达情况。结果 恩替卡韦组和联合中药组血清HBV DNA载量及GOT、GPT水平均明显低于模型组(P均<0.05),血清ApoB、VLDL水平均明显高于模型组(P均<0.05);联合中药组血清HBV DNA载量及GOT、GPT水平均明...     

健脾养血祛风方对脾虚慢性湿疹模型小鼠皮肤组织水通道蛋白3的影响

目的观察健脾养血祛风方对脾虚慢性湿疹模型小鼠皮损处水通道蛋白3(AQP3)含量的影响,并探讨其可能的作用机制。方法取健康雄性小鼠50只,随机分为正常组、模型组、阳性药组及中药高、低剂量组。采用小剂量反复DNCB涂抹及番泻叶致脾虚证建立小鼠脾虚慢性湿疹模型。中药组予健脾养血祛风方灌胃,阳性药组予盐酸左西替利嗪灌胃。免疫组化法检测病变皮肤组织中AQP3的表达,同时观察皮肤组织病理变化。结果病理观察结果显示,健脾养血祛风方对小鼠皮损处炎症损伤具有一定的修复作用。与正常组比较,模型组AQP3过度表达;与模型组比较,各给药组AQP3表达均明显下降,染色强度降低。模型组AQP3平均光密度值明显高于正常组(P0.01);与模型组比较,各给药组均能下调AQP3表达(P0.05)。结论健脾养血祛风方下调皮损中过度表达的AQP3可能是其治疗脾虚慢性湿疹的作用机制之一。     

疏肝健脾方药对非酒精性脂肪性肝病大鼠肝组织SREBP-1c mRNA及蛋白表达的影响

目的:观察疏肝健脾方药对非酒精性脂肪性肝病(NAFLD)大鼠肝组织SREBP-1c mRNA及蛋白表达的影响,并探讨其机制。方法:选雄性SPF级SD大鼠55只,随机分为模型组、正常对照组和3个中药干预组,分别为疏肝组、健脾组及合方组。除正常对照组外,各组采用脂肪乳剂灌胃8 w复制大鼠NAFLD模型,同时各药物干预组给予不同药物干预(10 mL/kg),正常对照组和模型组给予等体积蒸馏水灌胃。8 w后处死动物取肝组织,HE染色观察肝脏病理变化;RT-PCR方法检测肝组织SREBP-1c mRNA的表达;免疫组织化学方法检测肝组织SREBP-1c的蛋白表达水平和分布。结果:模型组大鼠肝组织SREBP-1c mRNA表达水平及SREBP-1c蛋白阳性表达率较正常对照组显著升高(P<0.01);各中药干预组大鼠肝组织SREBP-1c mRNA表达水平及SREBP-1c蛋白阳性表达率较模型组显著降低(P<0.05或P<0.01);疏肝组、健脾组大鼠肝组织SREBP-1c mRNA表达率较合方组显著降低(P<0.01)。病理观察显示疏肝、健脾组大鼠肝组织脂肪变最轻。结论:疏肝健脾方药可能是通过下调SREBP-1c mRN...     

健脾解毒活血法对脾胃湿热型幽门螺杆菌性胃炎小鼠胃部炎症的影响研究

目的:探讨健脾解毒活血法组方对脾胃湿热型幽门螺杆菌(Hp)相关性胃炎模型小鼠胃部炎症的影响。方法:以40只SPF级昆明种小鼠为实验对象,随机取10只作为空白组,剩余30只采用Hp菌液灌胃法建立脾胃湿热型Hp相关性胃炎小鼠模型,造模成功后,将其分为模型组、奥美拉唑钠组和中药组各10只。奥美拉唑钠组灌胃奥美拉唑钠混悬液10mg/kg体质量,中药组灌胃健脾解毒活血方药液18g/kg体质量,模型组灌胃同等量的0.9%氯化钠注射液,均每天1次,连续给药14d。观察各组血清白介素-1β(IL-1β)、胃泌素(GAS)以及胃组织Hp的感染情况。结果:模型组小鼠血清IL-1β、GAS的含量较空白组明显升高(P0.01,P0.05);与模型组比较,中药组和奥美拉唑钠组血清IL-1β显著降低(P0.05),但GAS则无明显差异。模型组小鼠胃组织Hp感染数较空白组明显增加;与模型组比较,中药组和奥美拉唑钠组胃组织Hp感染数显著降低(P0.05)。结论:健脾解毒活血方对IL-1β及Hp感染数有明显改善作用,但对GAS则没有明显影响。     

解毒护肝方抑制NLRP3炎症小体活化缓解对乙酰氨基酚诱导的小鼠急性肝损伤

目的:探讨解毒护肝方对对乙酰氨基酚(APAP)所致小鼠急性肝损伤(ALI)的保护作用及其机制.方法:60只C57BL/6N小鼠随机分为正常组、模型组、阳性药组以及解毒护肝方低、中、高剂量组.APAP造模同时,分别灌胃给予相应药物治疗,连续14 d.实验结束后,采集血清和肝组织,检测血清中丙氨酸氨基转移酶(ALT)、天门...     

凉血解毒利湿方对小鼠银屑病样皮损中过氧化物酶体增殖激活受体的影响

目的探讨凉血解毒利湿方治疗银屑病的可能作用机制。方法 50只BALB/c小鼠随机分为空白对照组、模型组、甲氨蝶呤组及中药高、低剂量组。除空白对照组外,其余各组连续6日外涂5%咪喹莫特乳膏建立银屑病模型,除模型组外,中药高、低剂量组分别给予凉血解毒利湿方42、21 g/(kg·d)灌胃,甲氨蝶呤组给予甲氨蝶呤片1 mg/(kg·d)灌胃,模型组和空白对照组给予0.4 ml蒸馏水,每日1次,共6天。第7天观察小鼠皮损变化情况;HE染色观察皮损组织形态学变化,光镜下测量表皮厚度;免疫荧光法检测小鼠皮损中增殖细胞核抗原(PCNA)有丝分裂细胞核数、有丝分裂细胞率、外皮蛋白、过氧化物酶体增殖激活受体α(PPAR-α)、过氧化物酶体增殖激活受体γ(PPAR-γ)、过氧化物酶体增殖激活受体β/δ(PPAR-β/δ)表达情况;Real-Time PCR、Western blot法分别检测小鼠皮肤中PPAR-α、PPAR-γ、PPAR-β/δmRNA、蛋白表达。结果模型组小鼠皮损鳞屑叠起、红斑色深、浸润较厚;与模型组相比,各给药组皮损症状均有明显改善。与空白对照组比较,模型组小鼠表皮细胞层增厚,有丝分裂细胞核数量均明显增多,有丝分裂细胞率显著升高。与模型组比较,甲氨蝶呤组及中药高、低剂量组PPAR-α、PPAR-γ蛋白表达升高,PPAR-β/δ蛋白表达降低,甲氨蝶呤组PPAR-γ、PPAR-β/δmRNA及中药高、低剂量组PPAR-β/δmRNA均明显降低(P0.05或P0.01)。与中药高剂量组比较,中药低剂量组PPAR-β/δmRNA表达降低(P0.01)。结论凉血解毒利湿方可调控银屑病小鼠皮损中PPARs表达,从而抑制表皮细胞过度增殖和分化,可能是其治疗银屑病的作用机制之一。     

解毒护肝方对药物性肝损伤大鼠TLR3/TNF-α/JNK2信号通路的影响

目的观察解毒护肝方对药物性肝损伤大鼠TLR3/TNF-α/JNK2信号通路的影响。方法采用对乙酰氨基酚灌胃制作大鼠肝损伤模型。将实验大鼠按体质量随机分为正常组、模型组、阳性对照组和解毒护肝方高、中、低剂量组,各给药组给予相应药液灌胃,正常组和模型组给予生理盐水灌胃。生化分析仪检测血清AST、ALT活性和总胆红素(TBIL)、直接胆红素(DBIL)含量;RT-PCR和Western blot分别检测肝组织JNK2基因和蛋白的表达;酶标仪检测肝组织匀浆肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)含量;免疫组化染色观察Toll样受体3(TLR3)蛋白表达。结果与正常组比较,模型组大鼠ALT、AST、DBIL、TNF-α水平显著升高(P0.01),JNK2 mRNA和p-JNK2、TLR3蛋白表达显著升高(P0.01);与模型组比较,解毒护肝方中、低剂量组大鼠AST、ALT活性显著降低(P0.05,P0.01),解毒护肝方低剂量组大鼠DBIL含量显著降低(P0.05),解毒护肝方高剂量组大鼠TNF-α含量显著降低(P0.01),解毒护肝方高、中剂量组大鼠JNK mRNA和TLR3蛋白表达均显著下调(P0.05,P0.01),p-JNK2蛋白表达有降低趋势,但差异无统计学意义(P0.05)。结论解毒护肝方对药物性肝损伤大鼠有明显的保护作用,以中、低剂量组效果最为明显,其机制可能与调控TLR3/TNF-α/JNK2信号通路有关。     

解毒祛瘀滋阴方对MRL/lpr狼疮小鼠CD4~+T细胞MeCP2信号通路的影响

目的探讨解毒祛瘀滋阴方治疗系统性红斑狼疮的可能作用机制。方法 30只MRL/lpr狼疮小鼠随机分成中药组、西药组、模型组各10只,10只C57BL/6小鼠作为对照组。中药组予0.5 g/ml浓度的解毒祛瘀滋阴方水煎剂灌胃,西药组予0.5 mg/ml浓度的醋酸泼尼松片混悬液灌胃,模型组和对照组予蒸馏水灌胃,均每次0.01 ml/g,每日1次,连续干预12周。干预结束后观察小鼠皮毛、活动及浅表淋巴结等一般状态,检测小鼠血清白细胞介素4(IL-4)、γ干扰素(IFN-γ)水平变化;HE染色染色观察肾脏病理改变;检测小鼠脾脏CD4~+T细胞甲基结合蛋白2(MeCP2)m RNA和蛋白的表达水平。结果与模型组比较,中药组小鼠肾脏病理损伤明显改善,血清IL-4、IFN-γ浓度水平降低,MeCP2 m RNA和蛋白表达水平明显升高(P0.05或P0.01),且中药组MeCP2蛋白及m RNA表达水平升高较西药组明显(P0.05或P0.01)。结论解毒祛瘀滋阴方可能是通过激活MeCP2信号通路,进而抑制系统性红斑狼疮的发病。     

微波消解-电感耦合等离子体质谱法测定脉络宁注射液中25种矿物质元素

目的建立微波消解-电感耦合等离子体质谱(ICP-MS)直接稀释测定脉络宁注射液中25种矿物质元素(Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V、As、Cd和Hg)的方法。方法分别对微波消解条件和测试条件进行考察;样品经微波消解后,采用电感耦合质谱仪测定25种矿物质元素,并对测定方法学进行考察。结果确定最佳消解条件为3步缓慢升温:400 W 80℃升温10 min,保留5 min;600 W 120℃升温10 min,保留5 min;900 W 200℃升温20 min,保留20 min;25种矿物质元素在各自的线性范围内线性关系良好,r≥0.999 6,精密度、稳定性和重复性试验的RSD均符合定量分析要求;加标回收率为94.7%~106.1%,RSD在0.34%~2.79%。脉络宁注射液中检测出Mg、Ca、Fe、Cu、Zn、Mn、Al、B、Ba、Co、Cr、K、Li、Mo、Na、Ni、P、Pb、Sr、Th、Ti、V,未检出As、Cd和Hg。结论该方法简便、迅速、准确,适用于脉络宁注射液中25种矿物质元素的同时测定。     

Die Lateralität und Lateralitätsinstabilität in der Diagnostik und Therapie

The disturbance and instability of laterality are obstacles to diagnostics and therapy. Correction prior to starting therapy is required. They also predispose toward defined health problems and unspecific diseases. Numerous research activities provide evidence of the relevance of undisturbed laterality in diagnostics and therapy. Techniques of testing and therapeutic corrections will allow for optimized therapy success.     

Yerba Mate or Paraguay Tea

正Beverages based on Ilex paraguariensis A.St.-Hil.are used in the south Brazil and other Latin American countries located at the so-called southern cone.It is known as chimarro or mate in south and southeast Brazil,tererêin     

Journal of Acupuncture and Tuina Science Instructions for Authors

The Journal of Acupuncture and Tuina Science, a journal with an international scope （IS SN 1672-3597, CN 31-1908/R, Bimonthly）, is embodied by ＇Springer Verlag＇ Database, Index Copernicus （IC） and Chinese Scientific and Technical Paper and Citations Data （CSTPCD）. You can search full text on http：//www, springerlink, com/content/1672 -3597.     

Ethnopharmacologic study of chinese rhubarb

This paper deals with an interdisciplinary study covering historic, botanical, phytochemical, pharmacological and clinical aspects of rhubarb and related species, to lay stress on the correlation between plant phylogeny, chemical constituents and purgative activity.

It was found that the official rhubarbs were exclusively restricted in the Sect. Palmata e.g. Rheum palmatum R. palmatum var. tanguticum R. officinale; the following criteria may serve as their standard, viz., the presence of sennoside derivatives and rhein, the occurrence of the reduced form of rhein and aloe-emodin, the leaves with any kind of palmate incision. Comprehensive multivariate analyses showed that there is a very close relationship between the leaf incision, existence of sennosides or rhein and purgative activity.     

基于ICP-MS法的加味左金丸中无机元素分析

目的 通过电感耦合等离子体质谱（inductively coupled plasma mass spectrometry,ICP-MS）法建立加味左金丸中Cd、Pb、As、Hg、Co、V、Ni、Cu、Li、Sb、Ba、Mo、Sn、Cr、Na、Mg、Al、Ca、Ti、Mn、Fe、Zn、Ga、Se、Sr、Tl共计26种无机元素的测定方法。方法 加味左金丸通过微波消解法处理后,根据相对分子质量的大小选择内标物,其中⁷Li、²³Na、²⁴Mg、²⁷Al、⁴⁰Ca、⁴⁸Ti、⁵¹V、⁵²Cr、⁵⁵Mn、⁵⁶Fe、⁵⁸Ni、⁵⁹Co、⁶³Cu、⁶⁶Zn、⁷⁰Ga、⁷⁵As、⁷⁷Se、⁸⁶Sr以⁷²Ge作为内标;⁹⁵Mo、¹¹⁴Cd、¹¹⁸Sn、¹²¹Sb、¹³⁷Ba以¹¹⁵In作为内标;²⁰²Hg、²⁰⁵Tl、²⁰⁸Pb以²⁰⁹Bi作为内标。对标准品溶液、空白溶液与供试品溶液进行分析,采用标准曲线法进行定量分析。通过ICP-MS法进行测定。结果 26种无机元素线性的相关系数r ≥ 0.999 6,检出限为0.001~1.500μg/L,定量限为0.01~5.00 μg/L,精密度与重复性试验的RSD均小于5%,平均回收率在82.64%~106.44%,RSD均小于5%。对3个厂家的12批样品进行了测定,26种元素的含量差异较大,其中Na、Mg、Ca、Fe 4种元素的含量比较高,均大于500 μg/g,Cd、Pb、As、Hg、Co、Li、Sb、Mo、Sn、Cr、Se、Tl的含量比较低,均小于1 μg/g。由结果可知,人体的常量元素,如Na、Mg、Ca的含量比较高,Cd、Pb、As、Hg等有害元素含量比较低。根据《中国药典》2020年版一部的要求,本品中Cd、Pb、As、Hg与Cu均符合规定。结论 该方法快速、准确,可以用于加味左金丸中无机元素的测定。     

Wirksamkeit der Akupunktur bei der Therapie von idiopathischen Schmeckstörungen: Eine randomisierte Placebo-kontrollierte Studie

Background

At present, the state-of-the-art medication in treating idiopathic taste disorder (gustatory dysfunction) is zinc. If zinc medication was unsuccessful, not tolerated or if it is contraindicated the persons affected can hardly be helped.

Objectives

In a randomized placebo-controlled clinical trial an attempt was made to determine the efficacy of acupuncture.

Methods

The treatment group was treated with acupuncture needles, the placebo group with an inactive acupuncture-laser. Endpoints of the study were changes in the taste test, the quality of life and the severity of symptoms of depression (BDI) as well as mood changes (ZMS). Satisfaction with the success of the respective therapy was also evaluated. Data were collected before and after therapy and again for the treatment group 10 weeks after completion of the treatment period.

Findings

Treatment with real acupuncture showed a significant improvement in gustatory function as well as a significant improvement in psychological symptoms. Greater satisfaction with the treatment results was evident in the treatment group. The treatment results of acupuncture remained stable over a period of ten weeks after completion of the treatment.

Conclusion

Acupuncture was effective in the therapy of idiopathic taste disorders and therefore it is an effective alternative when zinc medication is not tolerated or unsuccessful. But it is also suitable as a therapy of first choice. Acupuncture can be considered as an extension of the therapeutic spectrum in the difficult treatment of taste disorders.     

Erstaunliches Ergebnis einer Schädelakupunktur nach Yamamoto bei Hemihypsarrhythmie rechts

A girl, three and a half years old at the respective date, premature in 25th week of gestation, had been presented. On day three after birth, she had had a IVH III° right and left with severe involvement of parenchyma and other multiple complications, e. a. infant respiratory distress syndrome up to the 42nd day postpartum, and epileptic attacks with apnoeic events.     

Veränderungen der Herzratenvariabilität (HRV) während Low Level Lasernadelakupunktur: Placebokontrollierte Studie an 19 herzgesunden Probanden

Background

Experimental and clinical studies postulate a neurobiological mechanism of acupuncture by modulating central pain systems, neurohormones and -transmitters. It seems to be quite likely that the autonomic nervous system (ANS) plays an important mediating role in this neuromodulation.

Aim

In measuring heart rate variability (HRV), as a non-invasive approach to the ANS-function, the object of this study was to monitor probable acupuncture-induced changes of autonomic balance. The innovative technology of laser-needle acupuncture offers for the first time a stimulation method which fulfils the demands on a placebo-controlled acupuncture trial not requiring controversial sham acupuncture.

Method

19 healthy voluntary subjects underwent 3 treatments in randomized sequence, consisting of placebo-, 1-point- (PC 6) and simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle-stimulation over 20 minutes. Before, during and after placebo-/verum laser-needle acupuncture, HRV-data for normalized HF(high frequency)- and LF(low frequency)-Power (nHF, nLF) and their quotient nLF/nHF obtained by power spectrum analysis (FFT), were measured and analyzed statistically at 5 different time points (ANOVA repeated measures, p < 0,05).

Results

During both verum applications, the 1-point- (PC 6) as well as the simultaneous 3-point- (PC 6, LR 3, SI 3) laser-needle stimulation, significant changes of the ANS-activity were found in comparison to the placebo application. The most significant modification of HRV was an increase of nHF (p < 0,05 (PC 6), p < 0,0001 (PC 6, LR 3, SI 3)) as an expression of growing vagal activity during acupuncture.

Conclusion

Laser-needle acupuncture causes in 1-point-stimulation (PC 6) as well as in simultaneous 3-point-stimulation (PC 6, LR 3, SI 3) significant changes in HRV in comparison to placebo application. HRV-monitoring seems to be a suitable approach in exploration of acupuncture-induced changes of ANS and could possibly be established in combination with laser needle acupuncture as a standard for further scientific and clinical acupuncture investigations which are greatly needed.