狼疮肾炎患者血清IL-16水平的检测及其临床意义

目的：探讨狼疮肾炎患者血清白细胞介素－16（IL－16）水平变化及其临床意义。方法：狼疮肾炎患者48例，按照系统性红斑狼疮疾病活动指数（SLEDAI）分为狼疮肾炎活动组28例。狼疮肾炎静止组20例；另选非狼疮肾炎组12例，正常对照组10名，采用酶联免疫吸附法（ELISA）检测各组的血清IL－16水平。结果：狼疮肾炎活动组、静止组血清IL－16水平均较正常对照组显著增高（P＜0.05），狼疮肾炎活动组血清IL－16水平与SLEDAI成显著正相关（r=0.839、P＜0.01），与血清抗双链DNA抗体水平、尿蛋白及狼疮肾炎肾组织疾病活动指数呈正相关，但狼疮肾炎静止组与非狼疮肾炎组之间血清IL－16水平无统计学差异。结论：狼疮肾炎患者血清IL－16水平增高，可作为评价狼疮肾炎活动的参考指标。     

狼疮肾炎外周血单个核细胞MAPKERK1/2信号通路活化的观察

目的目的观察有丝分裂原激活的蛋白激酶-细胞外调节激酶（MAPK^ERK1/2）细胞信号通路在系统性红斑狼疮（SLE）并肾炎（LN）患者外周血单个核细胞（PBMC）活化状态，探讨MAPK^ERK1/2通路在LN患者免疫异常中的作用及临床意义。方法26例LN患者和21例正常健康对照者进入研究，取外周血分离PBMC进行体外培养，利用蛋白印迹法（Western Blot）测定PBMC MAPK^ERK1/2蛋白磷酸化活化水平，比较LN与正常对照PBMC MAPK^ERK1/2活化水平的差异，并分析MAPK^ERK1/2。以活化水平与临床狼疮疾病活动的关系。结果LN患者PBMC中MAPK^ERK1/2信号通路呈高度活化状态，与正常健康对照有显著性差异（P〈0．05）；MAPK^ERK1/2蛋白磷酸化活化水平与狼疮疾病活动评分（SLEDAI）、血清抗双链DNA抗体呈正相关关系（P〈0．06）。结论LN患者PBMC MAPK^ERK1/2以信号通路异常活化，并与临床狼疮疾病活动密切相关。     

白细胞介素类mRNA在儿童哮喘中异常表达的检测及意义

目的：研究白细胞介素－12（IL－12），IL－13在哮喘发病中的作用。方法：用逆转录聚合酶链反应（RT－PCR）法半定量分析了哮喘急性发作期及正常对照组儿童外周血单个核细胞（PBMC）中IL－12，IL－13mRNA表达水平的变化，同时对IgE水平进行了检测，结果：哮喘组IL－12 mRNA表达减少，IL－13 mRNA表达增多，病情越重，IL－12mRNA表达越少，IL－13 mRNA表达越多，无论IgE升高与否。与对照组比较，IL－12，IL－13mRNA表达，差异均有显著性，结论：IL－12，IL－13可能是构成气道慢性炎症的各类因素之一。     

双歧杆菌对裸鼠腹腔巨噬细胞产生细胞毒性效应分子的影响

目的 了解青春型双歧杆菌刺激裸鼠腹腔巨噬细胞后产生的IL－6、IL－12及TNF－α的水平。方法 以青春型双歧杆菌注射于裸鼠腹腔，体外获取腹腔巨噬细胞，分别滴加兔抗鼠IL－6、IL－12及TNF-α单抗，然后以FITC标羊抗兔IgG孵育，最后用激光共聚焦显微镜测定巨噬细胞发出的荧光强度。结果 双歧杆菌注射组裸鼠腹腔巨噬细胞产生的IL－6、IL－12及TNF－α水平均显高于对照组（P＜0．01）。结论 青春型双歧杆菌能激活巨噬细胞，使之分泌多量的IL－6、IL－12及TNF－α。     

血清IL-10和IL-12在自身免疫性甲状腺疾病中的表达特征

目的：研究自身免疫性甲状腺疾病中血清白细胞介素－10（IL－10）和白细胞介素－12（IL－12）的表达特征及其在免疫反应发生中的作用和机制。方法：对38例Graves病（GD）患者（18例未治疗的初诊患者，为GDa组；20例抗甲亢药物治疗的患者，为GDb组）、24例慢性淋巴细胞性甲状腺炎（HD）患者及22例正常者（对照组）检测了血清中IL－10，IL－12的表达水平及甲状腺功能的变化。IL－10和IL－12采用酶联免疫法（ELISA）测定。血清游离T3（FT3），游离T4（FT4）和促甲状腺激素（TSH）测定用化学发光免疫分析法。抗甲状腺球蛋白抗体（TgAb)和抗甲状腺微粒体抗体（TmAb)用放射免疫法。结果：GDa组的IL－10、IL－12均高于对照组（P＜0．05，P＜0．01），但以IL－12的增高占优势，IL－12／IL－10比值增高（P＜0．05）。GDb组的IL－12水平和IL－12／IL－10比值较GDa组显著降低（P＜0．05，P＜0．01），与对照组比较无显著差异。IL－10水平较GDa组有上升趋势但无统计学意义，但高于对照组（P＜0．01），HD患者IL－12水平和IL－12／IL－10比值均较对照组增高（P＜0．01，P＜0．01），较GDb组也显著增高（P＜0．05，P＜0．01），IL－10水平与对照组比较无显著差异，但显著低于GDb组（P＜0．05）。结论：GD患者，在甲状腺功能亢进状态时，由Thl细胞产生的IL－12水平和低表Th2细胞活力的IL－10水平均明显增高，提示Th1和Th2的表达均增高，细胞免疫和体液免疫均参入了甲亢的自身免疫反应过程。在HD患者也有IL－12水平的显著增高，表明了Th1细胞因子所介导的细胞免疫在HD的病理反应中起主导作用。在GD和HD患者，均表现有IL－12／IL－10比值的变化，提示Th1/Th2间的平衡紊乱可能对介导甲状腺自身免疫炎症反应的产生起关键作用。     

变应性鼻炎特异性免疫治疗对＊IL—6，IL—8，IL—12影响的临床观察——附70例报告

目的：研究特异性免疫治疗前后变应性鼻炎患者血清白细胞介素（IL）－6,IL－8,IL－12的变化及意义。方法：用酶联免疫吸附试验（ELISA）检测健康体检者（对照组）19名,变应性鼻炎（鼻炎组）70例特异性免疫治疗前后的血清IL－6,IL－8,IL－12及总免疫球蛋白E（TlgE)水平,结果：鼻炎组特异性免疫治疗前与对照组相比,IL－6,IL－8,IL－12均明显升高,P＜0．01,特异性免疫治疗后与治疗前相比,IL－8显著降低,P＜0．01,IL－6明显降低,P＜0．05,IL－12呈有降低,但无显著性差异,P＞0．05,特异性免疫治疗前IL－6,IL－8与TlgE呈正相关,IL－12与TlgE呈负相关,结论：特异性免疫的治疗使变应性鼻炎患者IL－6,IL－8,IL－12的分泌趋于正常。提示特异性免疫治疗有可能通过改善机体的免疫功能而发挥作用。     

狼疮性肾炎患者免疫功能指标的检测

摘要：目的：分析狼疮肾炎（LN）患者的免疫功能指标。 方法：选取65例SLE患者,其中35例并发肾炎为LN组,30例未并发肾炎为SLE无肾炎组,以30例体检健康者作为对照组,同时检测各组外周血T淋巴细胞亚群水平及相关血清学免疫指标。 结果：LN患者组外周血CD4+细胞水平、CD4+/CD8+比值及C3水平明显低于SLE无肾炎组及健康人对照组（P<0.05）,IgG、IgM、24 h尿蛋白定量、半胱氨酸蛋白酶抑制剂C（CysC）水平明显高于SLE无肾炎组及健康人对照组。 结论：LN患者T淋巴细胞亚群数量及补体水平均显著降低,与肾功能损害相关。     

狼疮性肾炎患者Th1／Th2细胞因子的研究

目的探讨狼疮性肾炎患者的发病与Th1／Th2优势活化状态之间的关系。方法采用酶联免疫吸附法（FLISA）测定32名狼疮性肾炎患者和10名健康人PBMC培养上清白细胞介素-4（IL-4）,白细胞介素-10（IL—10）,干扰素-γ,（IFN-γ）和白细胞介素-2（IL-2）水平。结果活动期狼疮性肾炎患者IL-4、IL-10水平明显高于非活动期、正常对照组,差异有显著性（P〈0．05）。活动期狼疮性肾炎患者IL-2水平明显低于非活动期、正常对照组。差异有显著性（P〈0．05）。IFN-γ水平3组间无显著性（P〉0．05）。结论Th2细胞因子介导的免疫应答在活动期狼疮性肾炎的发病机制中占主导地位,活动期狼疮性肾炎患者Th1细胞因子的细胞活化降低。     

联合检测C1q抗体、双链DNA抗体对系统性红斑狼疮的诊断价值

目的探讨联合检测C1q抗体、双链DNA（dsDNA）抗体对狼疮活动和狼疮性肾炎的价值。方法58例系统性红斑狼疮分为疾病活动和疾病稳定组、狼疮性肾炎和非狼疮性肾炎组，酶联免疫吸附法检测血清C1q抗体和dsDNA抗体水平，比较两抗体单个和联合检测对疾病活动和狼疮性肾炎的价值。结果C1q抗体和dsDNA抗体阳性对疾病活动的敏感性分别为63．88％和69．44％，特异性分别为81．82％和86．36％；两抗体联合对疾病活动的敏感性为83．33％，特异性为72．73％，两抗体的一致率为70．68％。疾病活动组两抗体阳性率和抗体水平显著高于疾病稳定组；两抗体与系统性红斑狼疮疾病活动指数、血沉、IgG、球蛋白水平显著正相关，与C3、C4及白蛋白水平显著负相关。狼疮性肾炎组dsDNA水平显著高于非狼疮性肾炎组，尿蛋白≥（＋＋）患者C1q抗体水平显著增高。结论C1q抗体和dsDNA抗体都是狼疮疾病活动的指标，两抗体都与狼疮性肾炎有关，联合检测可以提高疾病活动检出率。     

活动性狼疮肾炎患者外周血单个核细胞钙调神经磷酸酶活性的检测

目的：检测活动性狼疮肾炎（LN）患者外周血单个核细胞（PBMC）钙调神经磷酸酶（calcineurin．CaN）活性。方法：体外培养活动性LN患者PBMC，应用发色底物法检测胞浆CaN活性。结果：在单纯培养情况下，正常对照组和活动性LN组PBMC均出现一定量CaN活化，活动性LN组显著高于正常对照组[（46．08±5．58）nm01／mg·pr us（8．81±3．61）nmol/ng·pr，P〈0．01]；在PMA＋ionomycin刺激下，各组CaN活性均升高，活动性LN组CaN活性明显高于正常对照组[（69．34±12．59）nmol／mg·pr us（37．12±11．57）nmol／mg·pr，P〈0．011。结论：LN患者PBMc异常活化可能与CaN过度活化有关。[著者文摘]     

狼疮肾炎外周血单个核细胞LFA—1表达

目的检测淋巴细胞功能相关抗原-1(LFA-1)在狼疮肾炎外周血单个核细胞中的表达并对其临床价值进行探讨.方法通过分离外周血单个核细胞,利用流式细胞术检测了狼疮肾炎外周血单个核细胞LFA-1表达.结果狼疮肾炎活动组外周血单个核细胞LFA-1表达高于非活动组和健康对照组(7.49±1.17vs2.67±0.65、2.21±0.32,P值均小于0.01),而非活动组与健康对照组则无明显差异(P＞0.05).LFA-1表达与狼疮活动指数呈正相关(r=0.746,P＜0.01).与狼疮活动指标抗核抗体、抗双链DNA抗体呈正相关(r值分别为0.627、0.705,P＜0.01),与补体(C3)呈负相关(r=-0.538,P＜0.01).结论LFA-1分子与狼疮活动密切相关,临床上可以作为狼疮活动的参考指标.     

Defective cellular immune responses in lupus nephritis

To determine whether patients with systemic lupus erythematosus (SLE) and active nephritis have more profound defects in cellular immune responses, we studied cell-mediated immunity (CMI) in 12 patients with lupus nephritis (LN) and 11 patients with chronic mesangial proliferative glomerulonephritis (CGN) without renal insufficiency. The LN patients had a significant depression in CMI, characterized by impaired delayed hypersensitivity skin reactivity to keyhole limpet haemocyanin (KLH), decreased percentage of T cells and diminished lymphocyte transformation to phytohaemagglutinin (PHA). The levels of concanavalin A (Con A)-induced suppressor cell activity (SCA) were significantly lower in LN patients compared with those in normal controls and those in CGN patients. The levels of all CMI parameters for the 6 LN patients with the nephrotic syndrome (NS) were significantly more decreased when compared with those of the 6 LN patients without NS. In contrast, the CMI levels in CGN patients with or without NS did not differ from normal subjects. These findings may lend further insight into the understanding of the immunoregulatory defect in LN.     

Studies on synthesis of interleukin-6 and gammaglobulin in peripheral blood mononuclear cells of patients with systemic lupus erythematosus.

Peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE) proliferated spontaneously and secreted an elevated level of IgG compared with that of normal controls. However, the levels of interleukin-6 (IL-6) produced by PBMC from patients with SLE with or without pokeweed mitogen (PWM) stimulation showed no significant difference from those of normal controls. The levels of IL-6 secreted spontaneously from PBMC of SLE patients correlated inversely with the percent and the absolute number of CD19 positive cells in PBMC, but not with the levels of IgG and IgM secreted spontaneously from PBMC. There was no significant difference in the levels of IgG produced by PBMC stimulated with IL-6 and also in the levels of IL-6 synthetized by T and B cells between SLE patients and normal controls. These data suggest that IL-6 may not play an important role in the hypergammaglobulinemia in SLE.     

Urinary neutrophil gelatinase-associated lipocalin as a marker for disease activity in lupus nephritis

The neutrophil gelatinase-associated lipocalin (NGAL) has been emerging as a novel biomarker of acute kidney injury while its value in lupus nephritis is uncertain. The aim of this study was to assess urinary NGAL levels as a marker for disease activity in patients with lupus nephritis.This study included 70 systemic lupus erythematosus (SLE) patients; 50 with active lupus nephritis (LN) and 20 without as well as 20 matched controls. The neutrophil gelatinase-associated lipocalin (NGAL) in both serum and urine samples was measured by enzyme-linked immunosorbent assay (ELISA). Patients with active LN received standard treatment then assessed for response as well as the value of urinary NGAL (uNGAL). Our results revealed that, The SLE patients with or without LN had an elevated urinary NGAL as compared to controls (p?相似文献   

血清CXCL10、肾酶的水平变化与狼疮性肾炎病情活动的关系

目的 探讨狼疮性肾炎(LN)患者血清趋化因子配体10(CXCL10)、肾酶的变化及其意义.方法 回顾性选取2016年4月至2018年12月在廊坊市人民医院确诊的LN患者87例(LN组)、慢性肾炎患者90例(对照组).根据系统性红斑狼疮疾病活动度评分(SLEDAI-2000)将LN组分为活动组57例,非活动组30例;根据...     

Defective T-lymphocyte colony formation in patients with lupus nephritis

To determine whether patients with systemic lupus erythematosus (SLE) and active nephritis have more profound defects in cell-mediated immunity (CMI), we studied T-colony-forming cells (TCFC) in 12 patients with lupus nephritis (LN) and 14 patients with chronic mesangial proliferative glomerulonephritis (CGN) without renal insufficiency. We also examined the activity of T-colony-stimulating factor (TCSF) in media conditioned by phytohaemagglutinin (PHA) stimulated peripheral blood lymphocytes (PHA-LCM). The levels of TCFC and TCSF were decreased in patients with LN compared with those in normal controls and lower in LN patients with the nephrotic syndrome (NS) than in those without NS. In contrast, these CMI parameters in CGN patients with or without NS did not differ from normal subjects. TCSF activity for TCFC in both normal individuals and LN patients was removed from PHA-LCM with interleukin 2 (IL 2) receptor bearing cultured T cells. These in vitro findings suggest that IL 2 is the essential factor contained in PHA-LCM. Our observations may lend further insight into the understanding of the immunoregulatory defect in LN.     

Urinary fibronectin as an indicator of kidney fibrosis in nephritis]

AIM: To investigate clinicomorphological relationships between elevated urinary excretion of fibronectin (FN) and development of fibrosis in the kidney in patients with lupus nephritis (LN) and chronic glomerulonephritis (CGN). MATERIALS AND METHODS: Urinary FN excretion was measured at radial immunodiffusion in 54 LN patients. Of them, 15 patients had inactive LN, 39 patients had active LN varying in clinical forms. Urinary FN was also measured by passive hemagglutination in 36 CGN patients (11 inactive CGN and 25 active CGN cases). Biopsy specimens were obtained from 49 patients with active nephritis (43 with CGN and 6 with LN). FN deposits were studied immunohistochemically and morphometrically with determination of relative fibrosis area. RESULTS: Urinary FN excretion in patients with nephritis was higher than in healthy controls. In active CGN and LN the levels of FN were significantly higher than in inactive CGN and LN. The highest FN urinary concentrations were registered in patients with severe CGN and LN, especially in the presence of renal failure and arterial hypertension. Among them, the highest individual values were observed in patients with rapidly progressive nephritis. No positive correlations were found between the degree of the urinary FN excretion increment and degree of proteinuria. This suggests local-renal origin of most urinary FN. Morphologically, FN deposits were revealed in 73% of the biopsies. In most of the patients with severe nephritis both in CGN and LN there was a diffuse distribution of FN in the glomerules and interstitium. A correlation with a morphological nephritis type was absent, but existed between FN presence in the renal biopsies and relative area of interstitium (fibrosis). CONCLUSION: FN excreted in high amounts with urine in nephritis originates from the kidneys and reflects severity of fibrogenesis in the kidney.     

MCP-1在狼疮性肾炎患者血浆及尿中的检测水平及意义

目的检测狼疮性肾炎(LN)患者治疗前后尿及血浆中单核细胞趋化蛋白-1(MCP-1)的表达水平及其临床意义,并进一步探讨MCP-1的水平与狼疮性肾炎进展的关系。方法实验分成4组:14例疾病活动期狼疮性肾炎患者(A组)、10例非疾病活动期狼疮性肾炎患者(B组)、16例无肾脏损害的系统性红斑狼疮患者(C组)及10例健康对照组(D组)。采用酶联免疫吸附法(ELISA法)检测LN患者尿及血浆中MCP-1的水平。结果 1狼疮性肾炎患者尿MCP-1水平显著高于正常对照组(P0.05)及无肾脏损害的系统红斑狼疮组(P0.05)。2尿MCP-1水平在狼疮性肾炎疾病活动期较非活动期增高(P0.05)。3治疗达缓解后的狼疮性肾炎患者尿MCP-1水平较治疗前明显降低(P0.05),血浆MCP-1水平在各组之间的差异无统计学意义。4尿MCP-1水平与尿蛋白定量、尿素氮、尿白蛋白、肌酐清除率呈正相关。血浆MCP-1只与尿蛋白定量呈正相关,其他指标均无相关性。结论尿MCP-1的水平可能与狼疮性肾炎的疾病进展相关,尿MCP-1可能成为狼疮性肾炎诊断及预后的生物学标志。