白花蛇舌草LC-MS指纹图谱的研究

目的:建立白花蛇舌草的LC-MS指纹图谱。方法:采用LC-MS法,检测不同产地同一季节采收的10批样品色谱特征,与此同时和黄毛耳草(两者都属于茜草科耳草属植物)进行比较。结果:建立白花蛇舌草指纹图谱。结论:LC-MS指纹图谱具有特异性,可以评价和全面控制白花蛇舌草质量。     

白花蛇舌草配方颗粒的HPLC指纹图谱研究

目的建立白花蛇舌草配方颗粒的HPLC指纹图谱分析方法,为科学评价其质量提供依据。方法采用高效液相色谱法,以AKKZONOBEL Kromasil 100—5 C18（4．6mmx250mm,5μm）为色谱柱;以甲醇（A）、0．05％（体积分数）H3PO4（B）为流动相进行梯度洗脱;检测波长240nm;流速1．0mL·min^-1;柱温30℃。结果建立了白花蛇舌草配方颗粒的指纹图谱,确定了25个共有峰,10批白花蛇舌草配方颗粒样品相似度在0．9以上。结论该方法简便、准确、重复性好,能有效地对白花蛇舌草配方颗粒的质量进行评价。     

白花蛇舌草组织培养研究

目的为白花蛇舌草的药材生产提供优质的种苗。方法用植物组织培养方法对白花蛇舌草进行快速繁殖研究。结果选出白花蛇舌草的最佳愈伤化培养基为MS 2,4-D 4.0mg/L Kt 0.5 mg/L、最佳分化培养基为MS BA 3.0 IAA 0.4、生根培养基1/2MS IBA 0.4 mg/L,,并分生出大量的白花蛇舌草试管苗。结论为白花蛇舌草的快速繁殖研究提供了一定的科学依据。      

滇产白花蛇舌草生药学初步研究

目的：对滇产白花蛇舌草Oldenlondia diffusa（willd） Rond进行生药学研究。为建立白花蛇舌草的质量控制方法及开发利用提供生药学资料，为该药材的深入研究奠定基础。方法：采用性状鉴别、显微鉴别、理化鉴别的方法对白花蛇舌草进行生药学研究。结果：显微鉴别、理化鉴别结果与报道一致。结论：通过比较，滇产白花蛇舌草与报道的其他产地的白花蛇舌草组织结构相似，化学成分类似。     

辛夷挥发油类成分气相色谱一质谱仪指纹图谱研究

目的建立辛夷挥发油成分气相色谱-质谱（GC—MS）指纹图谱。方法采用水蒸气蒸馏法提取辛夷挥发油，用GC—MS进行成分分析。色谱柱：HP-5MS（50m×0．20mm×0．33μm）石英毛细管柱；载气：99．9％氦气；载气流速：1．0ml／min；质谱扫描范围：30～550amu。结果建立了辛夷挥发油类化学成分GC—MS指纹图谱，标定了19个共有指纹特征峰，方法学考察符合指纹图谱技术要求。结论本法可为辛夷药材质量控制提供科学依据。     

bcl—xl和p53在人肝癌Bel7402细胞中的表达及中药的干预作用

目的：从基因水平探讨白花蛇舌草多糖对体外培养的人肝癌Bel7402细胞凋亡诱导的作用及机制。方法：水提醇沉法提取白花蛇舌草多糖，人肝癌Bel7402细胞常规体外细胞培养，设定空白对照组、5-氟尿嘧啶阳性对照组、白花蛇舌草多糖组。HE染色法光镜下观察细胞形态改变，计算凋亡指数，采用MTT法检测白花蛇舌草多糖对癌细胞的生长抑制作用，RT—PCR法检测原癌基因bcl—xl、抑癌基因p53基因mRNA表达的变化。结果：白花蛇舌草多糖体外抑制人肝癌Bel7402细胞生长、促进细胞凋亡，凋亡指数达16．97％，以MTT显色法检测细胞抑制率达到61．5％，以RT—PCR半定量法检测，上调p53基因mRNA表达，降低bcl—xl基因mRNA表达，以上各指标与空白对照组相比均有显著差异。结论：白花蛇舌草多糖抑制人肝癌Bel7402细胞增长，诱导细胞凋亡，其分子机制可能与激活抑癌基因p53基因、抑制原癌基因bcl—xl基因表达有关。     

白花蛇舌草中多糖提取精制工艺的考察

目的：改进白花蛇舌草中多糖的提取工艺，并对所得多糖进行分离纯化。方法：将旋转蒸发仪（RE-52—05）进行改进，用于提取白花蛇舌草中的多糖成分。采用紫外分光光度法测定提取物中多糖的含量，以优化多糖的提取工艺；采用化学方法和薄层色谱法对所得多糖进行鉴别，并用柱层析对样品进行分离纯化。结果：通过以上方法得到白花蛇舌草中多糖的白色纯品。结论：采用真空提取，可减少白花蛇舌草多糖的提取过程中因高温带来的多糖的损耗，经柱层析分离纯化可得多糖纯品。     

白花蛇舌草体外诱导人肝癌HEPG2细胞凋亡的初步研究

目的：初步探讨白花蛇舌草对人肝癌HEPG2细胞的增殖抑制作用。方法：制备白花蛇舌草水提取液，体外培养人肝癌HEPG2细胞，MTT法体外细胞毒实验，琼脂糖凝胶电泳方法检测细胞凋亡。结果：白花蛇舌草浓度在10-80mg／mL时，抑制人肝癌HEPG2细胞生长，且随着剂量的增加抑制作用增强，电泳图片显示DNA梯形降解带纹。结论：白花蛇舌草通过诱导细胞凋亡对人肝癌HEPG2细胞具有增殖抑制作用。     

白花蛇舌草及水线草的紫外谱线组法鉴别

目的：鉴别白花蛇舌草及其混淆品水线草。方法：应用紫外谱线组法，测试两在不同极性溶剂中的紫外吸收光谱。结果：两的水浸液、石油醚浸液及氯仿浸液的紫外吸收光谱几乎一致，而无水乙醇浸液白花蛇舌草在229nm波长处有一吸收峰，水线草在此处没有吸收峰。结论：无水乙醇浸液229nm波长处吸收峰的有无可作为白花蛇舌草及其混淆品水线草的鉴别特征。     

紫外荧光法鉴别白花蛇舌草及两种常见混淆品

白花蛇舌草为茜草科植物。白花蛇舌草Oldenlandia diffusa(Wild)Roxb的干燥全草有清热解毒、利湿消痈等功效。近年用于癌症治疗，用量较大。笔者对本区抽样质检中发现，白花蛇舌草最常见的混淆品有两种，经鉴定为白花蛇舌草的同属植物伞房花耳草和纤花耳草，二者与白花蛇舌草的植物形态极为相似，差别甚微，单凭外观难以区分。目前，白花蛇舌草和纤花耳草的理化鉴别法采用薄层色谱法，齐墩果酸反应。该法操作繁琐，条件苛刻，一般基层单位较难掌握控制。本文采取紫外荧光法，以辅助鉴别白花蛇舌草及其混淆品。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.