糖基化终产物在白内障晶状体膜外在蛋白中累积的研究

研究糖基化终产物（ａｄｖａｎｃｅｄｇｌｙｃａｔｉｏｎｅｎｄｐｒｏｄｕｃｔｓ，ＡＧＥ）在老年性白内障发病中的意义。方法制备ＡＧＥ牛血清白蛋白免疫家兔获抗血清。用Ｓｅｐｈａｒｏｓｅ４Ｂ－ＢＳＡ和Ｓｅｐｈａｒｏｓｅ４Ｂ－ＢＳＡ－ＡＧＥ柱纯     

卵巢癌抗独特型单链抗体诱导特异性体液免疫应答的动物实验研究

目的：用卵巢癌抗独特型单链抗体６Ｂ１１ｓｃＦｖ代替肿瘤抗原免疫动物，观察是否诱导动物产生特异性体液免疫反应。方法：将６Ｂ１１ｓｃＦｖ交联钥孔虫戚虫血蓝素，辅以弗氏佐剂反复免疫ＢＡＬＢ／ｃ小鼠，制备抗血清。采用间接法ＥＬＩＳＡ，竞争抑制ＥＬＩＳＡ及免疫流式细胞法分析抗血清特性。结果：经ＥＬＩＳＡ检测显示，由６Ｂ１１ｓｃＦｖ刺激产生的抗抗独特型单链抗体（Ａｂ３）能与６Ｂ１１ｓｃＦｖ和卵巢癌组织抗原ＯＣ１６６９特异性结合。竞争抑制ＥＬＩＳＡ表明，Ａｂ３能有效抑制卵巢癌单抗ＣＯＣ１６６９（Ａｂ１）与ＯＣ１６６９的特异性结合。免疫流式分析结果可见，Ａｂ３能与表达卵巢癌抗原的人卵巢癌细胞系ＯＶ１细胞表面结合而不能与无卵巢癌抗原表达的人宫颈癌细胞系ＨｅＬａ细胞表面结合。从以上结果可以证明Ａｂ３与Ａｂ１的抗原结合特性相同。结论：６Ｂ１１ｓｃＦｖ能代替卵巢癌抗原诱导动物产生特异性体液免疫反应，具有模拟抗原的作用，为６Ｂ１１ｓｃＦｖ作为卵巢癌抗独特型单链抗体疫苗的实际应用提供依据     

抗磷酸酪氨酸抗体的制备、纯化和应用

目的 制备抗磷酪氨酸多克隆抗体,利用引抗体研究缺血再灌注对海马磷酸酪氨酸蛋白（ｐ－ｔｙｒ－ｐｒ）免疫反应性的影响。方法 以偶联磷酸化酪氨酸的牛血清白蛋白（ＢＳＡ）作为免疫原免疫兔获得抗血清。将磷酸酪氨酸偶联到溴化氰活化的ｓｅｐｈａｒｏｓｅ４Ｂ上。抗血清经到酪氨酸－ｓｅｐｈａｒｏｓｅ４Ｂ亲和柱纯化,所得抗体专一性强,并经ｄｏｔ ｂｌｏｔ鉴定。结果 抗体仅对酪氨酸磷酸化的蛋白质包括酪氨酸磷酸化的血清白     

免疫亲和色谱定向偶联方法的研究

将初步纯化属小鼠ＩｇＧ１亚类的抗Ｇ３单克隆抗体（ＭＧ３１－随机偶联于ＣＮＢｒ－Ｓｅｐｈａｒｏｓｅ４日凝胶,并成功地定向偶联于ｒＰｒｏｔｅｉｎＡ－ＳｅｐｈａｒｏｓｅＦＦ凝胶,两种偶联反应的结合率分别达到９１．７％和８６．６％。ＭＧ３１－ｒＰｒｏｔｅｉｎＡＳｅｐｈａｒｏｓｅＦＦ的抗原结合容量是ＭＧ３１－ＣＮＢｒ－Ｓｅｐｂａｒｏｓｅ４Ｂ的１．８倍,因此,单克隆抗体通过其Ｆｃ区与ｒＰｒｏｔｅｉｎＡ结合,留出正确定向的抗原结合位点,能够提高免疫亲和吸附剂的结合容量。     

利用卵巢癌抗独特型抗体制备的Ab3及其功能特性研究

利用卵巢癌抗原内影像型单克隆抗独特型抗体６Ｂ１１和１Ｈ１２代替肿瘤抗原，诱导动物产生特异性体液免疫反应。经ＥＬＬＳＡ检测证明，由抗独特型抗体（Ａｂ２）刺激产生的抗－抗独特型抗体（Ａｂ３）能与卵巢癌组织抗原ＯＣ１６６－９特异性结合。用Ｗｅｓｔｅｒｎ ｂｌｏｔ方法分析证明Ａｂ３与Ａｂ１的抗原结合特性相同。Ａｂ３血清还可通过抗体依赖性细胞介导的细胞介导的细胞毒作用特异性杀伤卵巢癌ＳＫＯＶ３细胞，表明其与     

抗hTSH抗独特型抗体的制备：——免疫原的分离纯化及鉴定

本文根据独特型－抗独特型理论探讨Ｇｒａｖｅｓ′病的发病机理，报道了对兔抗ｈＴＳＨＩｇＧ免疫原的分离，纯化及鉴定。首先用饱和硫酸铵粗提兔抗ｈＴＳＨ血清γ－球蛋白，继以葡萄球菌Ａ蛋白琼脂糖凝胶亲和层析柱（ＰｒｏｔｅｉｎＡｓｅｐｈａｒｏｓｅＣＬ４Ｂ）纯化ＩｇＧ，经十二烷基硫酸钠—聚丙烯凝胶电泳（ＳＰＳ－ＰＡＧＥ）鉴定，证实为ＩｇＧ纯品。再经ＴＳＨ偶联的亲和层析柱为免疫吸附剂，进一步分离出抗ＴＳＨＩｇＧ，然后用放射免疫分析（ＲＩＡ）检测证实保持了较高的抗体活性。     

人血清淀粉样蛋白P的分离,纯化及抗血清的制备

目的分离、纯化人血清淀粉样蛋白Ｐ（ＳｅｒｕｍａｍｙｌｏｉｄＰ，ＳＡＰ）；利用新西兰大白兔制备抗血清。方法新鲜人血清２００ｍｌ与等体积４％琼脂糖凝胶（Ｓｅｐｈａｒｏｓｅ－４Ｂ）混合，在钙离子存在条件下分离ＳＡＰ，然后用二乙基氨基乙基琼脂糖凝胶ＣＬ—６Ｂ（ＤＥＡＥ—ＳｅｐｈａｒｏｓｅＣＬ－６Ｂ）离子交换层析技术进一步纯化ＳＡＰ，用火箭电泳检测ＳＡＰ组分，用十二烷基磺酸钠聚丙烯酰胺凝胶电泳（Ｓｏｄｉｕｍｄｏｄｅｃｙｌｓｕｌｆａｔｅ－Ｐｏｌｙａｃｒｙｌａｍｉｄｅｇｅｌｅｌｅｃｔｒｏｐｈｏｒｅｓｉｓ，ＳＤＳ－ＰＡＧＥ）检测其纯度，经Ａ２８０（１％）＝１８２测定其含量。用纯化的ＳＡＰ与完全佐氏试剂混合注射于新西兰大白兔背部皮内，每次１００μｇ，间隔１０天，连续３次，第四次２００μｇ，ＳＡＰ总用量为５００μｇ，１０天后取血制备抗血清。结果从２００ｍｌ人血清中提取纯品２４ｍｇ，产率约４０％。抗血清经双向扩散法测定效价为１：１６。结论该改良法，操作简便，节省时间，但ＳＡＰ产率并不低。     

Graves患者TSH抗独特型抗体检测及其意义

根据自身免疫性甲状腺疾病发病机制，新的独特型－抗独特型免疫网络学说，用兔抗人ＴＳＨＡｂ检测ＴＳＨＡｂ_２。以正常人为对照，其结合率ｘ＋２ｓ为正常上限，大于此值为阳性。１００例ＴＲＡｂ阳性组有５２％患者ＴＳＨＡｂ_２阳性，５０例ＴＲＡｂ阴性组有４％患者ＴＳＨＡｂ２_阳性，两组有明显差异（Ｐ＜０．０５）。ＴＲＡｂ和ＴＳＨＡｂ_２正相关（ｒ＝０．５８２，Ｐ＜０．０５）。同时用制备的ＴＳＨ抗体吸附ＴＲＡｂ阳性血清后再测ＴＲＡｂ吸附前后有明显差异（Ｐ＜０．０５）。ＴＳＨＡｂ_２的检出为探讨Ｇｒａｖｅｓ’发病机制提供了一条新思路。     

HBV感染者血中大颗粒淋巴细胞的测定

分５组测定了乙型肝炎病毒（ＨＢＶ）感染者血中大颗粒淋巴细胞（ＬＧＬ）的含量，每组各３０例。其中（１）组：乙型肝炎表面抗原（ＨＢｓＡｇ）、ｅ抗原（ＨＢｅＡｇ）和核心抗体（ＨＢｃＡｂ）均为阳性；（２）组：为ＨＢｓＡｇ和ＨＢｅＡｇ阳性者；（３）组：为ＨＢｓＡｇ阳性、抗－ＨＢｅ和抗－ＨＢｃ阳性者；（４）组：为抗－ＨＢｓ阳性者；（５）组：为抗－ＨＢｓ和抗－ＨＢｅ阳性者。并且与对照组进行比较。结果表明，（１＿     

血清前－S_2蛋白检测的临床意义

采用ＥＬＩＳＡ法检测１０４例各类型乙型肝炎（乙肝）患者的血清前－Ｓ２抗原（Ｐｒｅ－Ｓ２Ａｇ）及其抗体（Ｐｒｅ－Ｓ２Ａｂ），结果表明，Ｐｒｅ－Ｓ２Ａｇ的出现与ＨＢｓＡｇ、ＨＢｅＡｇ、ＨＢｃＡｂ呈显著相关性（Ｐ值均＜０．００５），主要见于乙肝急性期及慢性乙肝患者，说明病毒复制活跃、传染性强。而Ｐｒｅ－Ｓ２Ａｂ阳性仅见于急性乙肝恢复期。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.