黄芩黄精等四种中药抗真菌实验研究

应用试管法对黄芩、黄精、虎仗、大黄等四种中药对六种真菌的抗真菌作用进行了研究。初步结果：黄芩ＭＩＣ为：Ｔｒ和Ｅｆ为６．２５ｍｇ／ｍｌ，Ｔｇ为１２．５ｍｙ／ｍｌ，ＳＳ为２５．ｏｍｇ／ｍｌ，Ｃｎ为５０．０ｍｇ／ｍｌ；黄精ＭＩＣ为：Ｔｒ、Ｅｆ、Ｔｇ和ＳＳ为５０．０ｍｇ／ｍｌ；虎仗的ＭＩＣ为：Ｅｆ１２．５ｍｇ／ｍｌ，Ｔｇ和Ｔｒ为２５．０ｍｇ／ｍｌ；大黄为ＭＩＣ为：Ｔｒ５０．０ｍｇ／ｍｌ。     

血β_2－微球蛋白在慢性肺心病缓解期判定肾功能减退的价值

对３０例慢性肺心病缓解期患者及５０例正常人采用ＲＩＡ法测定了血清的β_２－微球蛋白（β_２－ＭＧ），结果显示：慢性肺心病缓解期患者血清β_２－ＭＧ值为３．５±０．５８ｍｇ／Ｌ，较正常人血清β_２－ＭＧ值３．２４±０．４５ｍｇ／Ｌ为高（ｔ检验，Ｐ＜０．０１），肺心病缓解期患者中有１４例（Ａ组）血清β_２－ＭＧ值为４．２９±０．３８ｍｇ／Ｌ，超过正常上限，且血ＢＵＮ６．８ｍｍｏｌ／Ｌ，Ｃｒ１３２μｍｏｌ／Ｌ，Ｃｃｒ６４ｍｌ／ｍｉｎ；另１６例（Ｂ组）血清β_２－ＭＧ值（３．１２±０．３４ｍｇ／Ｌ）正常，血ＢＵＮ５．４ｍｍｏｌ／Ｌ，Ｃｒ１１４μｍｏｌ／Ｌ，Ｃｃｒ１０２ｍｌ／ｍｉｎ，两组β_２－ＭＧ及Ｃｃｒ比较有显著差异（ｔ检验，Ｐ＜０．０１）。提示血清β_２－ＭＧ测定在慢性肺心病缓解期患者中较ＢＵＮ、Ｃｒ能更早地发现肾功能减退。     

烧伤焦痂毒性提取物D1对培养心肌细胞L—型钙离子通道活动…

目的：研究烧伤焦痂毒性提取物Ｄ１对培养心肌细胞Ｌ－型钙离子通道活动的影响；方法：采用培养２ｄ－５ｄ的的大白鼠飞快主肌细胞，以Ｃｅｌｌ－ａｔｔａｃｈｅｄ方式记录通道电活动结果：正常大鼠皮肤脂质提取物Ｄ１在０．５ｍｇ／ｍｌ与１．０ｍｇ／ｍｌ时对钙离子通道活动无显著影响，在２．０ｍｇ／ｍｌ时可使开放活动有所加强，烧伤焦生提取物Ｄ１在０．５ｍｇ／ｍｌ、１．０ｍｇ／ｍｌ、２．０ｍｇ／ｍｌ时使通道开放活动显著     

丝裂霉素C动脉灌注对血管形态改变的研究

本实验采用剂量０．５ｍｇ／２ｍｌ及１．０ｍｇ／２ｍｌ的抗癌药丝裂霉素Ｃ对家兔颈外动脉行一次性灌注，于灌注后１、３、７ｄ分别进行光镜及透射电镜检查。实验结果表明：颈外动脉内灌注ＭＭＣ可造成该动脉系统不同分段的不同程度的损伤。光镜下动的损伤以内膜破坏性改变为特征；亚微结构的改变呈现ＥＣｓ脱落、内膜通透性增高及血栓形成。     

中草药体外抗淋球菌的实验研究

该文报道采用琼脂对倍稀释法，测定了１２种中草药体外抗淋球菌作用的效果。结果显示：黄莲、五倍子、诃子有效强的抗淋球菌作用，其ＭＩＣ５０和ＭＩＣ９０为０．６２５ｍｇ／ｍｌ和１．２５ｍｇ／ｍｌ。黄柏、黄芩、金银花、连翘、虎杖抗淋球菌作用次之，其ＭＩＣ５０和ＭＩＣ９０分别为１．２５ｍｇ／ｍｌ和２．５ｍｇ／ｍｌ。赤芍、秦皮抗淋球菌作用较弱，其ＭＩＣ５０和ＭＩＣ９０均为２．５ｍｇ／ｍｌ和５．０ｍｇ／ｍｌ。研究结果提示中草药可用于治疗淋球菌所致感染，但需进一步临床研究。     

五味子和头孢氨苄对大肠埃希氏菌及金黄色葡萄球菌抑制作 …

目的：观察五味子和头孢氨苄对大肠埃希氏菌和金黄色葡萄球菌的抑制作用。方法：试管稀释法。结果：五味子水煎剂（１ｇ／ｍｌ,ｐＨ＝２．８）对二株受试菌的ＭＢＣ分别是６２．５ｇ／Ｌ和３１．２５ｇ／Ｌ,而五味子水煎剂调ｐＨ为７．０后。其ＭＢＣ均大于５００ｇ／Ｌ。头孢氨苄对大肠埃希氏菌的ＭＩＣ和ＭＢＣ分别是４．８８ｍｇ／Ｌ和１５６．２５ｍｇ／Ｌ;对金黄色葡萄球菌的ＭＩＣ和ＭＢＣ分别是２．４４ｍｇ／Ｌ和７８．１     

异丙酚辅助硬膜外麻醉的临床观察

腹盆腔手术２００例行硬膜外阻滞，局部药为２％利多卡因１０ｍｌ＋０．３％地卡因１０ｍｌ＋１：２０万Ｕ肾上腺素，麻醉平面出现后，随机分组，异丙酚组１００例，术前５ｍｉｎ静滴异丙酚稀释（５％ＧＳ４份＋异丙酚１份），负荷量为１０ｍｇ／ｋｇ，维持量２￣４ｍｇ／ｋｇ。氟芬合剂组１００例，术前５ｍｉｎ静注氟芬合剂２￣３ｍｌ作为对照组。结果两组均能满足手术要求，术中Ｐ、Ｒ、ＭＢＰ、ＳＰＯ２、ＥＴＣＯ２等指标两组无     

高效液相色谱法测定双黄连泡泡糖中绿原酸含量

采用高效液相色法测定了双黄连泡泡糖中绿原酸的含量，色谱柱为ＮｏｖａｐａｋＣ１８流动相为四氢呋喃：２％醋酸水，检测波长为３２６ｎｍ，流速为０．７ｍｌ／ｍｉｎ。在２．２￣２２．０ｍｇ／Ｌ浓度范围内有良好线性关系。平均回收率为９８．６％，ＲＳＤ为２．９４％。     

青光眼小梁切除术中局部应用丝裂霉素的临床疗效观察

为改善青光眼小梁切除术后滤过泡的功能，随机将４４例（６０眼）各型中晚期患者，分为丝裂霉素（ＭＭＣ）组及生理盐水组（各３０眼）ＭＭＣ组小梁切除术中局部应用０．２ｍｇ．ｍｌ＾－１ＭＭＣ５ｍｉｎ术后追踪１～２个月，结果示：ＭＭＣ组中的功能性滤过泡的眼显著多于对照组：ＭＭＣ组术后眼压下降率明显大于对照组，且一年时ＭＭＣ组手术成功率显著高于对照组，提示ＭＭＣ是安全，效好的抗瘢痕形成药物。     

鹅绒藤的正性肌力作用与毒性

鹅绒藤茎部乳汁水溶性冻干粉（ＦＤＰＣＣ）１～４ｍｇ／ｍｌ，对离体蛙心具有洋地黄样正性肌力作用，大剂量时（６ｍｇ／ｍｌ）致中毒并使心跳停止于收缩期。对离体豚鼠左、右心房可产生依剂量性正性肌力作用，半数有效浓度（ＥＣ＿（５０））分别为１．７×１０￣（－２）与４．０×１０￣（－２）ｍｇ／ｍｌ，对心率影响则不明显。鹅绒藤乳汁冻干粉小鼠静脉注射（ｉｖ）的半数致死量（ＬＤ＿（５０））为０．２１±０．０２ｇ／ｋｇ。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

猪肺磷脂注射液联合经鼻持续气道正压通气对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性的影响

目的 探讨猪肺磷脂注射液联合经鼻持续气道正压通气(NCPAP)对呼吸衰竭早产儿的临床疗效及肌酸激酶同工酶活性(CK-MB)的影响.方法 选取呼吸衰竭早产儿80例,分为观察组和对照组各40例.对照组采用NCPAP给氧治疗,观察组给予NCPAP给氧联合猪肺磷脂气管内给药.观察两组患儿治疗前及治疗12h、24 h后PaO2、PaCO2、血氧饱和度(SaO2)、pH的变化情况,检测治疗前及治疗5d后血清CK-MB水平;评估两组患儿的临床治疗效果.结果 两组患儿PaO2、PaCO2、SaO2、pH比较,差异均有统计学意义(P＜0.05),其中观察组治疗后的PaO2、SaO2、pH均高于对照组,PaCO2则低于对照组.两组的PaO2、SaO2、pH均随观察时间延长而升高(P＜0.05),PaCO2均随观察时间的延长而降低(P＜0.05).观察组治疗有效率为87.5％,显著高于对照组的70.0％ (P ＜0.05).治疗5d后两组患儿血清CK-MB水平均较前降低(P＜0.05),且观察组明显低于对照组(P＜0.05).结论 猪肺磷脂注射液气管内给药联合NCPAP可以显著降低呼吸衰竭早产儿CK-MB的含量,提高治疗有效率,起到很好的呼吸循环支持作用.