红细胞膜定向包裹血红蛋白-白蛋白纳米粒的制备及评价

  目的  制备红细胞膜定向包裹的血红蛋白-白蛋白纳米粒（RBC-Hb/BSA-NP）,并对其进行表征和长循环能力的探究。  方法  通过溶剂蒸发法制备包含血红蛋白的白蛋白纳米粒（Hb/BSA-NP）,然后采用物理挤压的方式制备得到RBC-Hb/BSA-NP,并对其粒径、Zeta电位和外观形态进行表征;通过评价红细胞膜包覆白蛋白纳米粒的完整性筛选出红细胞膜的最佳用量;采用测量制剂表面唾液酸含量确定红细胞膜的正确朝向;使用荧光显微镜与流式细胞仪检测RBC-Hb/BSA-NP体外抗巨噬细胞吞噬的能力;最后通过体内药代动力学实验来评价纳米制剂在体内的长循环效果。  结果  制备的RBC-Hb/BSA-NP的平均粒径为（127.7±3.5） nm,平均Zeta电位为（?17.1±0.28） mV,具有清晰的核-壳结构,72 h内稳定性良好。0.8 mL的全血中提取出的红细胞膜能够刚好完整包覆1 mL Hb/BSA-NP（ρ_BSA=10 mg/mL）。与游离的红细胞相比,RBC-Hb/BSA-NP中的唾液酸含量无显著变化,表明红细胞膜正向包裹在纳米粒的表面。与普通白蛋白纳米粒（BSA-NP）和Hb/BSA-NP相比,RBC-Hb/BSA-NP可显著减少巨噬细胞摄取,且在体内的循环时间显著延长。  结论  成功制备了红细胞膜定向包裹的血红蛋白-白蛋白纳米粒,并证实该递送系统具有良好的长循环能力。     

紫杉醇甘草次酸修饰透明质酸纳米粒的制备及性能研究

优化紫杉醇甘草次酸修饰透明质酸（PTX/GA-HA）纳米粒的载药工艺,并系统评价其体内外特性。以载药量、包封率为评价指标,通过单因素考察优化甘草次酸修饰透明质酸纳米粒的载药工艺。制得的纳米粒粒径为（321.2±8.2）nm,荷负电;载药量和包封率分别为（31.2±0.8）%和（90.3±1.6）%。体外释放动力学研究显示,在偏酸性介质中,PTX/GA-HA纳米粒下具有更快的释药速度。同时,MTT实验显示其对多种肿瘤细胞具有杀伤作用,尤其对HepG2细胞的生长抑制作用最强。此外,细胞摄取实验表明,GA-HA纳米粒易被肿瘤细胞摄取。因此,PTX/GA-HA纳米粒具有优良的体内外特性,其成功制备将有助于提高抗肿瘤药物的肿瘤靶向治疗效果。     

吴茱萸碱脂质纳米粒的药代动力学和在体肠吸收特性研究

研究吴茱萸碱脂质纳米粒在大鼠体内的药代动力学和在体肠吸收特性。采用溶剂蒸发法制备吴茱萸碱脂质纳米粒,并测量其粒径电位。雄性SD大鼠分别单剂量灌胃给予吴茱萸碱和吴茱萸碱脂质纳米粒后（按吴茱萸碱计,质量分数为250 mg/kg）,在预设时间点取血并采用HPLC法测定血浆样品中吴茱萸碱的含量,用DAS 2.1.1软件分析药代动力学数据;建立大鼠在体单向肠灌流模型,研究吴茱萸碱脂质纳米粒在大鼠肠道内的吸收情况。结果显示,吴茱萸碱脂质纳米粒的平均粒径和电位分别为180.10 nm和-17.90 mV。吴茱萸碱和吴茱萸碱脂质纳米粒的药时曲线下面积分别为（862.60±14.03）和（4 084.31±17.21）μg/L·h,峰浓度分别为（163.40±13.27）和（616.90±21.04）μg/L。吴茱萸碱脂质纳米粒在各个肠段的吸收均显著高于吴茱萸碱（P＜0.05）,且其在结肠的吸收优于其他肠段。其在胃、十二指肠、空肠、回肠和结肠的吸收速率常数分别为（45.10±6.08）×10^-5,（48.20±1.21）×10^-5、（22.10±3.18）×10^-5、（59.10±1.11）×10^-5和（90.00±3.85）×10^-5 s^-1。其在十二指肠、空肠、回肠和结肠的有效渗透系数分别为（44.10±0.51）×10^-5、（17.21±0.77）×10^-5、（35.36±0.31）×10^-5和（40.33±0.34）×10^-5 cm/s。综上,吴茱萸碱脂质纳米粒增强了吴茱萸碱在大鼠肠道内的吸收,提高了吴茱萸碱在大鼠体内的口服生物利用度。     

胰岛素缓释载体聚乙二醇-聚己内酯-聚甲基丙烯酸-N,N-二乙氨基乙酯的制备及评价

目的 制备pH敏感聚乙二醇-聚己内酯-聚甲基丙烯酸-N,N-二乙氨基乙酯(mPEG-PCL-PDEAEMA)载胰岛素缓释纳米粒,考察其体外释放效果和体内降糖活性.方法 结合开环聚合反应和原子转移自由基聚合反应合成具有不同疏水链段的mPEG-PCL-PDEAEMA,用傅里叶红外光谱和核磁共振氢谱表征其结构;采用纳米沉淀技术制备聚合物载胰岛素纳米粒,动态光散射法测定粒径,透射电子显微镜观察其形态;BCA法测定载药情况,并考察其体外释放行为;建立糖尿病大鼠模型,监测给药后血糖水平.结果 在pH1.2~7.4时,聚合物纳米粒的粒径随pH增大而减小.以90%wt投药比制备mPEG5k-PCL13k-PDEAEMA10k和mPEG5k-PCL10k-PDEAEMA10k载胰岛素纳米粒时的包封率和载药率为最佳,包封率分别为(81.99±1.77)%和(53.12±0.62)%,载药率分别为(42.46±0.53)%和(32.34±0.26)%,粒径分别为181.9±6.67 nm和169±7.1 nm.体外释放结果显示聚合物载胰岛素纳米粒具有出色的缓释行为,并且随着疏水链段的增长,药物释放速度减慢.体内药效实验表明mPEG5k-PCL13k-PDEAEMA10k载胰岛素纳米粒能够在体内保持48 h的降血糖效果,较游离胰岛素的降糖作用时间明显延长.结论 pH敏感三嵌段聚合物mPEG-PCL-PDEAEMA有望成为理想的胰岛素缓释载体.     

紫杉醇白蛋白片段纳米粒的制备

目的：利用蛋白酶解和纳米粒技术在白蛋白片段的基础上筛选制备紫杉醇纳米粒的新型载体,并考察相关理化指标。方法：利用牛血白蛋白（Bovine serum albumin,BSA）酶解片段作为载体,分散乳化-旋蒸去溶剂法制备紫杉醇-BSA酶解片段-纳米粒,在外观、粒径分布、收率、包封率、载药量及体外释药性方面对样品进行考察。结果：BSA酶解后经粗分离得到4个片段组,选择第4组为候选载体,得到的纳米粒外观形态圆整、均匀,初制备纳米粒混悬剂平均粒径为150.2 nm,平均收率为（45.132±0.903）%,平均包封率为（50.246±0.712）%,平均载药量为（4.804±0.101）%,48 h累计释药82.4%。结论：利用白蛋白酶解片段制备紫杉醇纳米粒是可行的,为扩大紫杉醇纳米粒的载体来源、降低制备成本提供了可能。     

硫酸长春新碱聚乳酸羟基乙酸共聚物纳米粒的制备及其体外性质评价

目的制备硫酸长春新碱（VCR）聚乳酸羟基乙酸共聚物（PLGA）纳米粒（NPs）,研究其理化性质以及体外抗肿瘤活性。方法采用改良的复乳溶剂挥发法制备负载硫酸长春新碱的PLGA纳米粒,以透射电子显微镜观察纳米粒的形态,以激光粒度仪测定纳米粒的粒径和Zeta电位,以透析袋法研究其体外释放规律,以人乳腺癌细胞（MCF-7）为细胞模型,通过MTT试验考察载药纳米粒的细胞毒性。结果制备的VCR-PLGA NPs外观呈球形,平均粒径为（145.81±4.72）nm,Zeta电位为（-17.50±1.92）mV,包封率为（56.81±3.17）%,载药量为（2.79±0.18）%,体外释放规律符合双相动力学方程：Q=100-（72.19e-0.164 3 t＋29.26e-0.002 971 t）（R2=0.996 8）。载药纳米粒与原药相比可以增加细胞摄取而引起细胞毒性。结论初步建立了负载硫酸长春新碱的PLGA纳米粒系统,为体内抗肿瘤活性研究提供了依据。     

阿奇霉素固态类脂纳米粒的制备及其体外释药特性的研究

目的:以硬脂酸为载体材料制备阿奇霉素固态类脂纳米粒(AZM-SLN),并考察其体外释药特性。方法:采用乳化蒸发-低温固化法制备AZM-SLN;并对其形态、粒径、包封率、结构和质量、体外释药特性等进行研究。结果:得到的硬脂酸固态类脂纳米粒为类球形实体,粒径分布比较均匀,平均粒径dav=(17.75±5.00)nm;三批载药纳米粒的包封率为(81.57±1.33)%;体外释药符合Higuchi方程;用DSC分析证明纳米粒确已形成。结论:硬脂酸纳米粒有可能成为一种新型药物载体。     

RGD肽修饰紫杉醇聚合物纳米粒的制备及其药效学研究

制备靶向肽c(RGDyK)修饰的紫杉醇聚合物纳米粒,并对其体内外药效学性质进行评价。采用透析法制备靶向肽修饰的包载紫杉醇(PTX)的低相对分子质量肝素-全反式维甲酸聚合物(PTX-LHRyK)纳米粒,测定其粒度分布、Zeta电位、载药量和包封率等理化性质,通过体外细胞毒实验和体内药效学实验评价PTX-LHRyK纳米粒的抗肿瘤效果。制得的PTX-LHRyK纳米粒的粒径为(131.7±2.3)nm,Zeta电位为(-27.1±2.3)mV,载药量和包封率分别为(32.03±0.11)%和(84.84±2.63)%。随着孵育时间的延长,PTX-LHRyK纳米粒对B16F10细胞的毒性增加,孵育72 h后对B16F10细胞的IC₅₀为(41.6±7.2)ng/mL,LHRyK载体对B16F10细胞的存活率无显著影响。体内药效学研究显示,PTX-LHRyK纳米粒的抑瘤率达到75.28%,是混合药物溶液组的1.46倍,纳米粒制剂组的小鼠体重和相对脾重均无显著性变化。因此,PTX-LHRyK纳米粒粒径小,载药量高,可明显提高紫杉醇的抗肿瘤治疗效果,且降低药物的不良反应。     

洛伐他汀仿生纳米脂质体的制备、体外靶向性及家兔体内药代动力学

以洛伐他汀(LT)为模型药物,用高密度脂蛋白(HDL)脂质组分制备洛伐他汀纳米脂质体(LT-NLC),考察了LT-NLC的形态、粒径、Zeta电位、包封率以及结晶行为。将LT-NLC体外和HDL孵育获得载脂蛋白(apo)制备LT-NLC-apo(代表NLC体内起主要作用的组分),评价其体外逃避巨噬细胞吞噬和靶向到泡沫细胞的能力,并对其进行了家兔体内的药代动力学研究。结果表明,LT-NLC为球形或者椭圆形的粒子,粒径(14.6±2.0)nm,Zeta电位(-32.3±0.4)mV,包封率(96.2±1.3)%。体外研究显示内源性的LT-NLC-apo能逃避巨噬细胞的吞噬并靶向到泡沫细胞,药代动力学数据进一步证明LT-NLC可有效逃避吞噬,延长体内循环时间。不含蛋白的仿HDL组分的NLC有望成为传递亲脂性抗动脉粥样硬化药物到泡沫细胞的有效工具。     

双响应性透明质酸碳量子点-明胶纳米递药系统的构建及抗肿瘤效果评价

  目的  通过构建pH和基质金属蛋白酶（matrix metalloproteinase, MMP）双响应性粒径可变纳米递药系统,协同提高化疗药物在肿瘤组织的高效滞留和高效穿透,增强肿瘤治疗效果。  方法  构建了透明质酸（hyaluronic acid, HA）碳量子点（carbon quantum dots, CD）偶联明胶纳米粒（gelatin nanoparticle, GNP）,通过pH敏感的亚胺连接化疗药物阿霉素（doxorubicin, DOX）,得到GNP@HA-CD-DOX纳米粒并进行表征,考察粒径变化能力、释药行为、血液相容性、细胞摄取和肿瘤球深层穿透能力、体内肿瘤分布以及治疗效果。  结果  GNP@HA-CD-DOX纳米粒粒径为（162.93±2.55） nm,在MMP处理下可降解释放出粒径约40 nm的HA-CD-DOX。该纳米粒DOX载药量为（4.94±0.22）%,DOX可以在肿瘤微环境和溶酶体中响应低pH释放。GNP@HA-CD-DOX无明显溶血现象;与MMP-2共孵育后粒径减小,能够明显提高细胞摄取和肿瘤球中的深层穿透。GNP@HA-CD-DOX在荷瘤小鼠模型上表现出优于小粒径HA-CD-DOX的肿瘤分布和抗肿瘤能力,且安全性较好。  结论  该pH和MMP酶双响应粒径可变的纳米递药系统协同提高药物在肿瘤的滞留和深层穿透,提高了抗肿瘤效果,为肿瘤治疗提供了新的思路。     

ANKRD7 and CYTL1 are novel risk genes for alcohol drinking behavior

Background  Alcohol dependence (AD) is a complex disorder characterized by impaired control over drinking. It is determined by both genetic and environmental factors. The recent approach of genome-wide association study (GWAS) is a powerful tool for identifying complex disease-associated susceptibility alleles, however, a few GWASs have been conducted for AD, and their results are largely inconsistent. The present study aimed to screen the loci associated with alcohol-related phenotypes using GWAS technology.

Methods  A genome-wide association study with the behavior of regular alcohol drinking and alcohol consumption was performed to identify susceptibility genes associated with AD, using the Affymetrix 500K SNP array in an initial sample consisting of 904 unrelated Caucasian subjects. Then, the initial results in GWAS were replicated in three independent samples: 1972 Caucasians in 593 nuclear families, 761 unrelated Caucasian subjects, and 2955 unrelated Chinese Hans.

Results  Several genes were associated with the alcohol-related phenotypes at the genome-wide significance level, with the ankyrin repeat domain 7 gene (ANKRD7) showing the strongest statistical evidence for regular alcohol drinking and suggestive statistical evidence for alcohol consumption. In addition, certain haplotypes within the ANKRD7 and cytokine-like1 (CYTL1) genes were significantly associated with regular drinking behavior, such as one ANKRD7 block composed of the SNPs rs6466686-rs4295599-rs12531086 (P = 6.51×10^–8). The association of alcohol consumption was successfully replicated with rs4295599 in ANKRD7 gene in independent Caucasian nuclear families and independent unrelated Chinese Hans, and with rs16836497 in CYTL1 gene in independent unrelated Caucasians. Meta-analyses based on both the GWAS and replication samples further supported the observed significant associations between the ANKRD7 or CYTL1 gene and alcohol consumption.

Conclusion  The evidence suggests that ANKRD7 and CYTL1 genes may play an important role in the variance in AD risk.

     

一氧化氮供体型心脑血管药物研究的实践与思考

一氧化氮（nitric oxide，NO）作为信使物质和/或效应分子在心血管、神经、免疫等系统中发挥着极其重要的作用。NO生成不足或信号传导异常与多种疾病的形成和发展密切相关，直接或间接调控体内NO水平及其相关代谢途径的研究已成为生物医学和药学领域研究的热点。本文介绍了体内NO的产生及其在心脑血管中的生理、病理作用，综述了本课题组在NO供体型心脑血管药物及相关药物研究方面所开展的工作，并对今后NO供体型心脑血管药物研究发展方向进行了展望。     

上海市闵行区流动人口婚育观念与行为的研究

应用现况调查方法,从上海市近效城乡结合部随机抽取２个乡镇（每个乡选取１个镇）作为调查单位,对２４８８例外来育龄流动妇女进行调查,着重调查婚姻和生育情况、生育意愿与生育决策、理想子女数和对子女性别的偏爱、节育状况、人流状况等。结果显示：由于国家计划生育政策的认真贯彻实施,外来流动人口的婚育观念及节育模式发生了改变,但有一些人仍保留着传统的婚育观念,提示应加强这些流动人口的计生宣教和计生服务。     

降压0号治疗高血压对抑郁情绪的影响

目的 研究降压0号对高血压患者抑郁情绪的影响。方法 采用现场实验的方法,将研究对象分为降压0号组和常规治疗组,进行为期1年的降压治疗。研究对象在基线和随访1年末均自填衡量抑郁程度的Zung量表。结果 降压0号组和常规治疗组分别纳入1445例和1414例研究对象。随访1年末两组血压达标率分别为80.6%和81.8%（P=0.439）。随访期间,两组量表评分均略有增加（P均<0.05）但变化幅度小且差异无统计学意义（P=0.429）,两组有抑郁倾向者比例均无明显变化（P均>0.05）。结论 与其他常规降压药物相比,未发现降压0号治疗高血压对患者的抑郁情绪有明显影响。     

Regulatory effect of Langchuang serial recipes on T-lymphocyte subsets Th and Tc in patients with systemic lupus erythematosus

Objective： To study the principle of clearing Fei （肺）, cooling blood, and detoxification as well as nourishing yin and moisening Fei （abbr. as CCD-NM） in regulating the levels of peripheral T-lymphocyte subsets Th and Tc cells to explore its mechanism for lowering the incidence of infection in patients with systemic lupus erythematosus iSLE）. Methods： Sixty SLE patients without complicated infection were assigned to the treatment group and the control group, 30 in each group. The control group was treated with Western medicine alone, while the treatment group was treated with the same program of Western medicine, but additionally administered with either Langchuang No.1 （狼疮 Ⅰ号） or 2 （狼疮 Ⅱ 号）, serial concerted Chinese recipes, applied respectively in patients in the active stage or in the resting stage. The total time of treatment for both groups was 1 year. Further, a healthy control group was set up with 20 healthy subjects. The expressions of Thl, Th2, and Tcl and Tc2 cells in peripheral blood were detected and compared with those in the healthy control group. Results： （1） As compared with the healthy control group, ratios of Thl/Th2 and Tcl/Tc2 in SLE patients, whether complicated with infection or not, were significantly lower （P〈0.05 or P〈0.01）. （2） Comparison between patients with complications and those uncomplicated with infection showed that the two ratios and Thl expression were lower and Tc2 was higher in the former than those in the latter （all P〈0.05）. （3） Ratios of Thl/Th2 and Tcl/Tc2 increased after treatment in patients of both the treatment group and the control group （P〈0.05 and P〈0.01）, but the changes in the treatment group were more significant （P〈0.05）. Conclusion： The principle of CCD-NM could regulate the Th and Tc subsets toward equilibrium in SLE patients, which might be one of the mechanisms of action for alleviating complicated infection.     

Effect of Jieze No.1(洁泽Ⅰ号)on Cervicitis Caused by Ureaplasma Urealyticum and on Ureaplasma Urealyticum in vitro

Objective:To observe the clinical therapeutic effect of Jieze No.1(洁泽Ⅰ号)on cervicitis caused by ureaplasma urealyticum and its inhibitory effect on ureaplasma urealyticum (Uu)in vitro.Methods:A total of 393 patients suffering from cervicitis induced by ureaplasma urealyticum without other complications were randomly assigned to 3 groups,the combined treatment group:140 patients treated with Chinese herbs Jieze No.1 by vaginal lavage,30 min each time,once a day for 10 consecutive days and oral administration of Azithromycin,1.0 g once every 72 h for three times;Jieze group:115 patients were treated with Jieze No.1 alone by vaginal lavage,30 min each time,once a day for 10 consecutive days;and the Azithromycin group:138 patients were treated with oral administration of Azithromycin,1.0 g once in 72 h for three times.All the patients were treated for 1 therapeutic course and condom were used for contraception during the treatment course.The Uu patients were examined again after 21 days of treatment.The therapeutic effect on cervicitis was observed.The experimental study of Jieze No.1 on the Uu strain separated from the secretion of the urogenital tract was also observed.The minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of the Uu were investigated.Results:The total effective rate of the combined group was 85.3%,showing a significant difference compared with the Jieze group(67.8%)and the Azithromycin group(60.3%,both P＜0.01).There was no statistical significance between the latter two groups(P＞0.05).The clearing rate of Uu in the combined group was 78.4%,that of the Jieze group was 60.9%and the Azithromycin group was 47.9%.The combined group also showed a significant difference in comparison with the other two groups(all P＜0.01).Especially for the drugresistant strain,the clearing rate of Uu reached 48.1%in the combined group,42.1%in the Jieze group,and 16.1%in the Azithromycin group,respectively.The clearing rate of Uu for the drugresistant strain in the former two groups had significant differences in comparison with the latter (P＜0.01,P＜0.05),while there was no significant difference between the former two(P＞0.05).The range of MIC and MBC of Jieze No.1 to the drug-resistant strain of Uu was 15.62-250.00 mg/mL.To the non-drug-resistant MIC and MBC strain,it was 15.62-125.00 mg/mL.For the drug-resistant strain,MIC50 was≤31.25 mg/mL,MBC50 was≤62.50 mg/mL,MIC90 was≤1 25.00 mg/mL and MBC90 was≤250.00 mg/mL.For the non-drug-resistant strain,MICso was≤31.25 mg/mL,MBC50 was≤62.50 mg/mL,MIC90 was≤62.50 mg/mL and MBC90 was≤1 25.00 mg/mL.Conclusion:Jieze No.1 combined with Azithromycin can effectively treat cervicitis caused by Uu.The laboratory study confirms that Jieze No.1 has an inhibitory effect on ureaplasma uraalyticum strain.It has a remarkably effective therapeutic effects on drug-resistant strains,which is worthy of further research.     

洁泽Ⅰ号抗解脲支原体药效实验及其对解脲支原体宫颈炎的治疗作用

目的研究洁泽Ⅰ号体外抗解脲支原体（Uu）的作用及其治疗解脲支原体宫颈炎的临床疗效。方法测定洁泽Ⅰ号对解脲支原体临床分离株的最低抑菌浓度（MIC）、最低杀菌浓度（MBC）。310例完成临床观察符合诊断标准无合并症的解脲支原体宫颈炎患者，随机分为三组，A组：阿奇霉素联合洁泽Ⅰ号阴道灌洗治疗组102例；B组：洁泽Ⅰ号阴道灌洗治疗组87例；C组：口服阿奇霉素治疗组121例。三组治疗期间安全套避孕，均治疗满一疗程，停药21d后复查解脲支原体并行妇科检查，观察三组临床疗效。结果洁泽Ⅰ号对解脲支原体耐药株MIC范围为：15．62～250．00（mg／ml，以下均同此单位），MBC的范围为：15．62-250．00；非耐药株MIC范围为：15．62～125．00；MBC范围为：15．62～250．00；耐药株MIC50≤31．25、MBC50≤62．50、MIC90≤125．00，MBC90≤250．00，非耐药株MIC50≤31．25、MBC50≤62．50，MIC90≤62．50，MBC90≤125．00。临床疗效观察：总有效率A组85．3％；B组：67．8％，C组：60．3％，A组与B组、C组相比差异有极显著性意义（均P〈0．01），B组与C组相比差异无显著性意义（P〉0．05）。宫颈分泌物Uu转阴率A组78．4％，B组60．9％，C组47．9％；A组与B组、C组相比差异有极显著性意义（均P〈0．01），B组与C组相比差异无显著性意义（P〉0．05），对耐药菌株Uu转阴率，A组48．1％，B组42．1％，C组16．1％，A组与B组相比差异无显著性意义（P〉0．05），A组与C组相比差异有极显著性意义（P〈0．01），B组与C组相比差异有显著性意义（P〈0．05）。结论体外实验表明洁泽Ⅰ号能有效抑制解脲支原体的生长，联合阿奇霉素对解脲支原体宫颈炎有较好的治疗效果，特别是对耐药菌株疗效有显著优势，值得进一步研究。     

胆道直接灌注胆石溶解剂的体外实验研究

通过对一系列的溶石剂进行遴选实验 ,遴选出疗效确切 ,更适宜于临床治疗的胆石溶解剂。方法 :采用体外溶石法 ,比较胆石相对减重率 ,最终确定胆石溶解剂。结果 :在模拟胆囊条件下 ,桔油Ⅰ号油溶石速度最快、效果最理想     

Effect of Jieze No.1 (洁泽 号) on cervicitis caused by ureaplasma urealyticum and on ureaplasma urealyticum in vitro

Objective： To observe the clinical therapeutic effect of Jieze No. 1 （洁泽Ⅰ号） on cervicitis caused by ureaplasma urealyticum and its inhibitory effect on ureaplasma urealyticum （Uu） in vitro. Methods： A total of 393 patients suffering from cervicitis induced by ureaplasma urealyticum without other complications were randomly assigned to 3 groups, the combined treatment group： 140 patients treated with Chinese herbs Jieze No.1 by vaginal lavage, 30 min each time, once a clay for 10 consecutive clays and oral administration of Azithromycin, 1.0 g once every 72 h for three times; Jieze group： 115 patients were treated with Jieze No.1 alone by vaginal lavage, 30 min each time, once a day for 10 consecutive days; and the Azithromycin group： 138 patients were treated with oral administration of Azithromycin, 1.0 g once in 72 h for three times. All the patients were treated for 1 therapeutic course and condom were used for contraception during the treatment course. The Uu patients were examined again after 21 clays of treatment. The therapeutic effect on cervicitis was observed. The experimental study of Jieze No. 1 on the Uu strain separated from the secretion of the urogenital tract was also observed. The minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of the Uu were investigated. Results： The total effective rate of the combined group was 85.3%, showing a significant difference compared with the Jieze group （67.8%） and the Azithromycin group （60.3%, both P〈0.01）. There was no statistical significance between the latter two groups （P〉0.05）. The clearing rate of Uu in the combined group was 78.4%, that of the Jieze group was 60.9% and the Azithromycin group was 47.9%. The combined group also showed a significant difference in comparison with the other two groups （all P〈0.01）. Especially for the drugresistant strain, the clearing rate of Uu reached 48.1% in the combined group, 42.1% in the Jieze group, and 16.1% in the Azithromyc     

祛痰活血开窍法对脑组织缺血性损伤的保护及其机制的研究

目的：探讨具有祛普活血开窍功效的中药复方脑Ⅱ号对急笥脑缺血性损伤的保护作用及其机制。方法：采用电镜技术及放射性核素示踪法等技术，从功能与结构方面进行研究。结果；脑脉Ⅱ号能减轻急性脑缺血大鼠大脑皮层组织结构的损伤程度，提高缺血脑组织的ＳＯＤ，ＧＳＨ－ＰＸ活性，并减少ＬＯＰ含量，能增加脑组织的＾３Ｈ－脱氧葡萄糖含量。