三种方法检测尿中的有形成分（白细胞红细胞及管型）结果分析

目的：三种方法UF-50全自动尿沉渣分析法与尿干化学分析法显微镜检测尿常规红细胞，白细胞及管型。方法：随机收集住院患者的晨尿500份，先用尿干化学分析法检测再用UF-50尿沉渣分析仪自动进样模式检测，最后再用Olympus显微镜镜检。将三种方法检测结果作比较。结果：500份标本中，UF-50尿沉渣分析仪检测管型阳性的有69例，显微镜镜检管型阳性的有17例，UF-50尿沉渣分析仪检测管型阳性率13．8％，显微镜检测管型阳性率3．4％，将UF-50沉渣分析仪与显微镜检测管型阳性率进行χ^2检验（P〈0．05）两者相差显著。UF-50全自动尿沉渣分析仪与尿干化学分析仪联合检测尿中红细胞结果阳性率40．2％，显微镜检测阳性率42％，UF-50全自动尿沉渣分析仪与尿干化学分析仪联合检测尿中白细胞阳性率43％，显微镜镜检阳性率43．4％，经矿检验两者相差不显著（P〉0．05）。结论：UF-50尿沉渣分析仪对尿中管型的检测存在假阳性，UF-50尿沉渣分析仪，干化学分析仪与显微镜检测尿中白细胞和红细胞结果较一致。     

不同尿液检测方法结果分析

为探讨尿液分析的自动化应用,对尿液分析仪器的干扰因素造成的假阳性结果进行分析,采用UF-50全自动尿沉渣分析仪和尿干化学仪联机后结果与显微镜镜检结果进行分析比较。结果,UF-50全自动尿沉渣分析仪所测红细胞和管型假阳性率较高,红细胞阳性数580例、假阳性数222例;管型阳性数509例、假阳性数166例;红细胞和管型假阳性率分别为38.28%和32.61%。白细胞和上皮细胞假阳性率较低,白细胞阳性数342例、白细胞假阳性数24例;上皮细胞阳性数90例、上皮细胞假阳性数9例;白细胞和上皮细胞假阳性率分别为7.01%和10.00%。通过UF-50全自动尿沉渣分析仪、尿干化学仪和显微镜三者交叉互检,可排除假阳性结果,使尿液分析准确化,为临床提供可靠的诊断依据。     

粘液丝、上皮细胞对UF-50型尿沉渣分析仪测管型的影响

UF-50型尿沉渣分析仪是使用流式细胞技术的尿液有形成分分析仪。该仪器识别红细胞、白细胞、细菌、真菌的能力强。但还不能准确鉴别各种管型。为了探讨尿液中各种上皮细胞和尿液粘液丝对UF-50型全自动尿沉渣分析仪测管型的影响，我们对725份尿液标本，用显微镜与用UF-50型全自动尿沉渣分析仪分别检测，并对测定结果对照分析。其结果表明，正常尿液和管型尿液标本，UF-50仪测定结果与镜检相符，但有一部分含有上皮细胞及粘液丝的标本易被误认为管型。因此在用UF-50型尿沉渣分析仪作分析时，当分析结果提示有管型时，应进行离心镜检。以减少误诊，提高检验质量。     

UF-500i尿液分析仪检测尿液有形成分的对比分析

目的探讨UF-500i尿液分析仪对尿液主要有形成分(红细胞、白细胞、管型)检测的准确性.方法随机收集我院门诊患者新鲜晨尿标本480份,并额外特别选取外观异常尿液60份,分别采用UF-500i尿液分析仪和普通光学显微镜镜检,结果进行比较分析.结果在各类尿样的检测中,UF-500i尿液分析仪测定的红细胞、白细胞阳性率与显微镜镜检比较,均无统计学意义,管型阳性率均明显高于显微镜镜检阳性率.在混浊尿和明显含黏液丝尿液的检测中假阳性率更高.结论 UF-500i尿液分析仪测定尿液有形成分,管型易出现假阳性,尤其对于外观异常尿液,在经仪器检测后,必须用显微镜复检.     

UF-50尿沉渣分析仪检测准确性的探讨

目的评价UF-50尿沉渣分析仪对尿液主要有形成分红细胞、白细胞和管型检测的准确性。方法随机收集我院门诊和住院患者新鲜晨尿400份,用尿沉渣分析仪定量分析后再用显微镜镜检,将结果进行比较分析。结果UF-50尿沉渣分析仪对红细胞、白细胞和管型检测的阳性率均明显高于显微镜镜检阳性率。结论UF-50尿沉渣分析仪对尿液检测容易受到多种干扰因素的影响,假阳性率高,不能完全替代显微镜镜检,尤其是UF-50尿沉渣分析仪提示阳性的标本更应进行显微镜检查,以确保检验结果的准确无误。     

UF-50尿沉渣分析仪与常规尿液分析联合检测的应用价值

目的：探讨UF-50尿沉渣分析仪与干化学分析、显微镜检查联合检测尿液的应用价值。方法：用UF-50全自动尿沉渣分析仪、Miditron尿＋项分析仪和显微镜分别对850例尿液标本进行检测，并对3种检验结果进行分析比较。结果：UF-50法测红细胞、管型和小圆上皮细胞假阳性率较高，分别为16．8％、83．5％和14．3％；测白细胞假阳性率较低，为1．86％。干化学法测红细胞阳性数较高，白细胞阳性数偏低。结论：UF-50尿沉渣分析仪与干化学分析、显微镜检查联合应用，可以排除假阳性结果，使尿液分析标准化．为临床提供可靠依据。     

UF-50全自动尿沉渣分析仪各检测项目干扰因素分析

目的:探讨UF-50尿沉渣分析仪与显微镜镜检结果的符合情况及影响因素。方法:随机收集门诊患者的晨尿标本836份,用UF-50尿沉渣分析仪与显微镜分别检测尿液中红细胞、白细胞、管型,然后将两者的检测结果进行比较。结果:836份标本中UF-50尿沉渣分析仪检测红细胞阳性的有224例,假阳性率为21.4%,假阴性率为2.9%;白细胞阳性的有205例,假阳性率为5.4%,假阴性率为1.9%;管型阳性的有168例,假阳性率为30.9%,假阴性率为0.7%。结论:UF-50尿沉渣分析仪只能定位于过筛作用,尿沉渣的显微镜检查可对尿中的有形成分进行最直观的分析,避免仪器检测造成的某些误诊、漏诊。     

尿沉渣分析仪假阳性结果分析在临床中的应用

目的:探讨尿液分析的标准化应用,对尿液分析仪器的干扰因素造成的假阳性结果进行分析。方法:采用UF-50全自动尿沉渣分析仪和尿干化学仪联机后结果与显微镜镜检结果进行分析比较。结果:UF-50全自动尿沉渣分析仪所测红细胞和管型假阳性率较高,分别为38.84%和32.94%;白细胞和上皮细胞假阳性率较低,分别为7.23%和11.49%。结论:通过UF-50全自动尿沉渣分析仪、尿干化学仪和显微镜三者交叉互检,可排除假阳性结果,使尿液分析标准化,为临床提供可靠的参考依据。     

探讨尿沉渣镜检时白细胞与红细胞的筛选标准

目的 探讨尿沉渣镜检时白细胞与红细胞的筛选标准.方法 采用干化学分析+尿分析仪自动分析+尿沉渣显微镜检查检测尿液标本,并对结果进行分析.结果 UF-50检测结果正常且干化学结果异常或UF-50检测结果中白细胞＜20/ul、红细胞＜ 30/ul时尿沉渣镜检红细胞、白细胞数均在参考值范围内.结论 UF-50检测结果中,白细胞＞20/ul、红细胞＞30/ul时,需做尿沉渣镜检.     

UF-100尿沉渣分析仪与尿沉渣镜检的重要性

目的:分析UF-100尿沉渣分析仪与尿沉渣镜检联合运用对尿液中红细胞、白细胞和管型检测的重要性.方法:对1 000例患者的尿液标本进行尿沉渣分析、干化学分析和显微镜镜检,并进行比较.结果:426例尿沉渣分析仪检测异常者中48例属于正常,假阳性率为10.8 %,尿液中细菌、结晶、黏液丝、酵母样菌等是影响尿沉渣分析仪检测结果的主要因素.结论:UF-100全自动尿沉渣分析仪与显微镜镜检联合应用,可以提高检测精确度.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.     

Scientific principles and rigorous processes should be followed in developing clinical guidelines for therapeutic interventions of integrative medicine

A clinical guideline for the therapeutic interventions of integrative medicine may be defined as a written document which states a series of recommendations on therapeutic interventions of integrative medicine for a special disease or condition. The guideline may provide assistance to medical professionals in making clinical decisions aimed at improving the clinical outcome of patients and reducing the costs of medical care（~＇4~. Recommendations issued by a guideline should be based on the best available evidence in both Western and Chinese medicine. For fulfilling this purpose, the development of clinical guidelines for therapeutic interventions in the field of integrative medicine should follow scientific principles and undergo a rigorous processes.