R-HPLC测定兔血清中头孢氨噻肟及其去乙酰代谢物浓度

建立了反相高效液相色谱法(R-HPLC)测定12只家兔血清中头孢氨噻肟(CTX)及其主要代谢产物去乙酰头孢氨噻肟(dCTX)的方法。本法测定CTX及dCTX的最低检出浓度分别为0.3μg/ml及0.5μg/ml;CTX的线性范围为0.5～20.0μg/ml;dCTX的线性范围为1.0～20.0μg/ml;药物及内标头孢唑啉的回收率均在90％以上。操作简便、准确。     

微波消化-分光光度法快速检测人发铝

介绍了一种将微波消化与分光光度法联用,快速测定人发中铝含量的新方法.在选定的实验条件下,测定铝离子的线性范围为:0～3.5μg/ml,其相对标准偏差为5.1％(n=6),回收率为99.3％。经30例正常人发铝含量分析,结果((?)±s)为16.9±9.6μg/g,与文献值基本一致。本法比目前其它方法更简单、更快速,可作为人体内铝水平的检测指标。     

芘丁酸-二氧化硅敏感膜测定正己烷中的单质硫

目的:为研究测定石油制品中硫化物的光纤化学传感器提供实验基础。方法:采用溶胶-凝胶法制备芘丁酸-二氧化硅敏感膜(试剂项),根据荧光多元猝灭响应原理测定正己烷中单质硫的含量。结果:单质硫在0.75～15.00μg/ml浓度范围内与lg(F0/F)成线性关系,r=0.999 3。检出限为0.75μg/ml,平均回收率为(103.2±7.5)％。结论:用芘丁酸-二氧化硅试剂相测定正己烷溶液中的单质硫含量可取得较好的精密度、回收率和可逆度。     

石墨炉原子吸收光谱法测定水中微量铝

目的采用石墨炉原子吸收光谱法,加入重铬酸钾基体改进剂,测定水中微量铝。方法通过改进铝的灰化温度、原子化温度以及基体改进剂来测定水中铝的含量。结果本方法检出限为0．85μg/L,相关系数为0．9996;线性范围为0～100μg／L;相对标准偏为1．08％～1．4％,加标回收率为91％-102％。结论显示研究方法干扰少,灵敏度高,精密度和准确度均能满足测定要求,适合水样中铝的测定。     

氢化物发生原子吸收光谱法测定天然水中痕量锑方法探讨

本文对氢化物发生原子吸收光谱测定天然水中痕量锑的方法进行了探讨．该法具有简便、快速、灵敏、准动等优点．本文对有关实验条件和数据进行了验证，得出最低检出限为2ng．若取100ml水样分析，其最低检出浓度0．02μg／L．10、20、40ng锑含量水平标准溶液和含锑3．18ng天然水样精密度（以CV计）为1．2％～13．7％，水样加标回收率为95％～105％．该法对河水、湖水、自来水．井水等10种水样测定,发现多数水样含量为0．012～0．086μg／L.     

对-氨基偶氮苯法测定酒中甲醇含量

本文提出一种间接测定酒中甲醇的、灵敏的分光光度法。甲醇被氧化成甲醛后用对-氨基偶氮苯和SO_2显色,在酸性介质中比色测定。甲醇含量在0～300μg/25ml范围遵循比耳定律,最大吸收波长为505nm,检出极限为1μg/ml。酒中一些常见有机物不干扰测定。     

高效液相色谱荧光-光化学衍生法测定固体牛乳制品中黄曲霉毒素含量

目的 采用免疫亲和柱-光化学衍生联合高效液相色谱荧光检测器测定奶酪、奶片、奶粉和奶酪棒4类固体牛乳制品中的黄曲霉毒素B₁、B₂、G₁、G₂的含量。方法 对2021年9月—12月市场采集的24份固体牛乳制品样品进行水浴均质，采用乙腈超声提取、粗去蛋白，离心移取上清液并定容，一次性过免疫亲和柱净化，5ml甲醇和水溶液分别淋洗，2ml甲醇洗脱，洗脱液氮吹浓缩，1ml乙腈涡旋复溶后过膜测定。结果 黄曲霉毒素B_l、B₂、G₁和G₂线性浓度范围分别为0.015～6.000μg/L、0.023～6.000μg/L、0.030～6.000μg/L和0.030～6.000μg/L,相关系数均大于0.999,方法检出限为0.06～0.12μg/kg,方法回收率为80.7%～93.6%,相对标准偏差在2.1%～7.8%(n=6)。结论 采用高效液相色谱荧光-光化学衍生法能够满足黄曲霉毒素B₁、B₂     

瞬时甲基化气相色谱法测定苯妥英和扑痫酮的体液浓度

本文报道用瞬时甲基化气相色谱法,在改装的国产100型气相色谱仪上,分离和测定体液中同时存在的苯妥英(DPH)和扑痫酮(PD)或其中的一种药物。在血样中应用本法的最低检测浓度DPH为1μg/ml;PD为2μg/ml。用甲基苯妥英(M-DPH)作内标,DPH的线性范围为2～50μg/ml,r=0.9973,方法回收率为99.95～105.4％,天内精密度CV(％)<1.71％,天间精密度CV(％)<2.33％;PD的线性范围为4～40μg/ml,r=0.9975,方法回收率为98.90～103.9％,CV(％)<6.49％。用本法测定DPH的血药浓度和分光光度法比较,r=0.9824,测定值y(uv)=0.02964+0.9074x(GLC)。用本法监测37例DPH的血清样品,多数样品的血药浓度低于10μg/ml,测定值和临床反应基本一致。用本法也能测定药物的唾液浓度,从而计算病人的DPH血浆蛋白结合率,测定7例的结果为91.03±3.06％,与文献报道的89～93％相一致。     

HPLC测定枸橼酸西地那非片中西地那非及其有关物质的含量

目的建立RP-HPLC测定枸橼酸西地那非片中西地那非及其有关物质的含量.方法以乙腈-水(44∶56,v/v)为流动相,HypersilODS-2色谱柱(5μm,200mm×4.6mm)为固定相,流速为1ml/min,检测波长为292nm.结果西地那非在20～80μg/ml浓度范围内线性关系良好,RSD为1.27%,平均回收率为99.48%,最低检测限为0.1μg/ml.结论方法可用于枸橼酸西地那非片及有关物质的含量测定.     

用氨敏电极测定水中氨氮含量方法的探讨

作者系统地研究了用氨气敏电极测定水样中氨氮含量的方法,结果表明加酸调pH<2保存水样,加EDTA消除金属离子干扰,可获得满意效果。方法的回收率范围为90～104％,变异系数≤3.4％,测定范围为10~(-1)～10~(-4)μg/ml,检出限为0.13μg/ml,与纳氏比色法测定结果比较无差异(t=0.3775,P>0.5)。本法所需设备价廉,操作简便快速,结果准确可靠,可直接测定有色或浑浊水作中氨氮含量。     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.