高压氧治疗脑卒中的剂量研究

观察２２例脑卒中患者在高压氧治疗不同时期体内自由基代谢物质含量的变化。每天用０．２ＭＰａ高压氧稳压治疗４０ｍｉｎ，连续治疗２０天。结果发现，经高压氧连续治疗１０天，患者血浆ＬＰＯ含量的增加和红细胞ＳＯＤ、ＧＳＨＰｘ活性的下降都不明显（Ｐ＞０．０５），仅红细胞明显降低（Ｐ＜０．０５）；连续治疗２０天后，患者血浆ＬＰＯ含量明显增高，红细胞ＳＯＤ和ＧＳＨＰｘ活性及ＧＳＨ含量显著下降（Ｐ＜０．０５）。建议     

精神分裂症的脂质过氧化研究

目的：研究精神分裂症病人活性氧自由基发生及脂质过氧化的生成情况。方法：本研究应用超氧化物歧化酶超微量快速测定法、ＤＴＢＮ直接法、二硫双硝基苯甲酸定量测定法和ＴＢＡ法测定了精神分裂症病人４０例（Ⅰ型２３例，Ⅱ型１７例）和健康对照者４０人血中ＳＯＤ，ＧＳＨ－ＰＸ活力，ＧＳＨ和ＬＰＯ含量。结果：精神分裂症血中ＳＯＤ活力明显高于对照组（Ｐ＜０．０５），ＧＳＨ含量极显著低于对照组（Ｐ＜０．０１），而ＧＳＨ－ＰＸ活力、ＬＰＯ含量无明显变化（Ｐ＞０．０５）。其中Ⅱ型病人ＧＳＨ含量降低有更为明显的趋势。结论：对精神分裂症的脂质过氧化研究具有重要含义。     

NIDDM合并视网膜病变患者红细胞聚集性变化及其影响因素

本文测定了 ＮＩＤＤＭ合并视网膜病变患者２１例、无视网胰病变患 者１９例及正常对照２０例的红细胞聚集性有关参数。的果显示，ＮＩＤＤＭ视网膜病 变组的低切全血粘度、红细胞聚集指数均显著高于无视网膜病变组及对照组（Ｐ＜ ０．０１）。ＮＩＤＤＭ组和合并视网膜病变组的血沉及血沉方程Ｋ值均显著高于对照组 （Ｐ＜０．０１）。ＮＩＤＤＭ视网膜病变组的血浆纤维蛋白原水平及糖化血清蛋白指数 均显著高于无视网膜病变组和对照组（Ｐ＜０．０５，Ｐ＜０．０１）。ＮＩＤＤＭ视网膜病变 组血清白蛋白及超氧化物歧化酶（ＳＯＤ）均显著低于无视网膜病变组和对照组（Ｐ ＜０．０５，Ｐ＜０．０１）．ＮＩＤＤＭ视网膜病变组红细胞聚集指数与血浆纤维蛋白原、 糖化血清蛋白指数呈显著正相关（ｒ值 ０．４５２、０．４４６，Ｐ＜０．０５）．与血清ＳＯＤ、白蛋 白呈显著负相关（ｒ－０．４６２、－０．４３９，Ｐ＜０．０５）．结果提示，ＮＩＤＤＭ合并视网膜 病变患者上细胞聚集性增高，这种增高与血中纤维蛋白原水平增高，蛋白质非酶糖 基化增加、ＳＯＤ及白蛋白水平降低有关．     

急性心肌梗塞QRS积分与严重并发症,酶学及血液流变学的关系

采用Ｗａｇｎｅｒ简化后的３７点／２９分ＱＲＳ积分系统，对按照ＷＨＯ标准诊断的１６０例急性心肌梗塞患者发病后一、二、三、七天分别进行心电图ＱＲＳ积分，观察积分与严重并发症、酶学、血液流变学的关系。第二、三天心电图积分＞３组泵衰、心性死亡率显著高于≤３组（Ｐ＜０．０１），积分越高，预后越差。心电图ＱＲＳ积分与ＳＬＤＨ（第一、二、七天，分别ｒ＝０．６１、０．５５、０．４２）、ＳＧＯＴ（第二天，ｒ＝０．８３）、ＳＣＰＫ（第三天ｒ＝０．６８）、α－ＨＢＤＨ（第七天，ｒ＝０．７３）呈正相关。第一天积分与全血低切比粘度、红细胞电泳时间呈正相关（ｒ＝０．４９和０．５８）。     

冠状动脉病变程度与血脂及其亚组分、超氧化物歧化酶关系的研究

目的：探讨血脂、脂蛋白及超氧化物歧化酶（ＳＯＤ）与冠状动脉病变程度的关系。方法：选择冠状动脉造影患者３０例，分为正常组（１１例）、单支组（１０例）和多支组（９例）；测定胆固醇、甘油三酯、脂蛋白及其亚组分和ＳＯＤ含量。结果：３组患者间血清甘油三酯和胆固醇水平相近；多支组高密度脂蛋白胆固醇／低密度脂蛋白胆固醇（ＨＤＬＣ／ＬＤＬＣ）比值低于正常组（Ｐ＜０．０５）。单支组和多支组ＨＤＬＣ均低于正常组（Ｐ均＜０．０１），而ＬＤＬＣ均高于正常组（Ｐ＜０．０５或Ｐ＜０．０１）。正常组ＳＯＤ为（２０３．４±５７．７）ｋＵ／Ｌ，高于单支组〔（１２９．４±７１．６）ｋＵ／Ｌ，Ｐ＜０．０５〕和多支组〔（９５．９±５３．３）ｋＵ／Ｌ，Ｐ＜０．０１〕。ＳＯＤ与ＬＤＬ呈中度负相关（ｒ＝－０．４４８，Ｐ＜０．０５），ＳＯＤ与ＨＤＬ（ｒ＝０．６９６，Ｐ＜０．０１）和ＳＯＤ与ＨＤＬＣ（ｒ＝０．３９９，Ｐ＜０．０５）均呈正相关。结论：血脂、脂蛋白、脂蛋白亚组分及ＳＯＤ与冠状动脉病变程度有一定的联系，ＨＤＬ、ＨＤＬＣ、ＬＤＬ、ＬＤＬＣ和ＨＤＬＣ／ＬＤＬＣ是预测冠状动脉粥样硬化性心脏病发病的重要易患因子。     

磁处理水与藻酸双脂钠对高脂血症大鼠的影响

目的 探讨磁处理水（简称磁水）与藻酸双脂钠（ＰＳＳ）治疗高脂血症（ＨＬＰ）是否有协同效应,为治疗高脂血症的供新途径。方法 采用高胆固醇饲料饲养大鼠,造成ＨＬＰ模型。用酶法测量大鼠血清胆固醇（ＴＣ）,甘油三酯（ＴＧ）,高密度脂蛋白－胆固醇（ＨＤＬ－Ｃ）,低密度脂蛋白－胆固醇（ＬＤＬ－Ｃ）并对大鼠血液流变学指标进行检测。结果 磁水组和ＰＳＳ组大鼠血清ＴＣ、ＴＧ、ＬＤＬ－Ｃ、全血低切（２０ｓ＾－１）与高切（８０ｓ6-1)比粘度ηｂ,全血还原粘度ηｒ都显著降低（与高脂对照组比较Ｐ〈０．０５或０．０１）。此外,ＰＳＳ还具有增高ＨＤＬ－Ｃ及ＨＤＬ－Ｃ／ＴＣ,降低血浆比粘度的作用（Ｐ〈０．０５）,而磁水虽有此作用,但无统计学意义。磁水＋ＰＳＳ组与磁水组和ＰＳＳ组比较血清ＴＣ、ＴＧ、ηＢ、显著降低（Ｐ〈０．０５）,此外,ＨＤＬ－Ｃ     

飞行人员疗养前后血浆尿液过氧化脂质测定

目的测定飞行人员疗养前后血浆、尿液过氧化脂质（ＬＰＯ）含量，旨在评定疗养效果。方法随机抽取２０例飞行人员，以入院疗养两天内作为疗养前，疗养至２０天时间为疗养后，测定疗养前后血浆、尿液ＬＰＯ含量。结果结果发现疗养后比疗养前血浆ＬＰＯ下降、尿液ＬＰＯ含量升高（Ｐ＜０．０５），疗养后血浆ＬＰＯ下降和尿液ＬＰＯ升高负相关（ｒ＝－０．６３８，Ｐ＜０．０５）。结论血浆、尿液ＬＰＯ可以作为疗养效果评定的客观指标。     

旋磁场并高压氧对脑卒中患者自由基代谢的影响

旋磁场并高压氧对脑卒中患者自由基代谢影响的研究结果表明，采用０．１ＭＰａ高压氧，每日１次，４０分钟，连续治疗１０次为１疗程，高压氧升高血浆过氧化脂质（ＬＰＯ）含量和抑制红细胞超氧化物歧化酶（ＳＯＤ）活性的作用不明显（Ｐ〉０．０５），但能明显降低红细胞谷胱甘肽（ＧＳＨ）含量和谷胱甘肽过氧化物酶（ＧＳＨｐｘ）的活性（Ｐ〈０．０５）。旋磁场，磁片两块异名极并置，磁感应强度为０．２５Ｔ，旋转（２５００ｒ／     

去甲斑蝥素致大鼠多脏器功能失常综合征的动态观察

目的：探讨多脏器功能失常综合征（ＭＯＤＳ）病程中重要脏器功能的变化以及与氧自由基的关系。方法：用ＳＤ大鼠制作ＭＯＤＳ模型,动态检测脏器功能及血清超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＳＨ Ｐｘ）和丙二醛（ＭＤＡ）的变化。结果：血清丙氨酸氨基转移酶（ＡＬＴ）、肌酸激酶及其同工酶（ＣＫ 和ＣＫ ＭＢ）和肌酐（Ｃｒ）均明显升高,其中ＣＫ ＭＢ于１２ 小时明显升高,１８ 小时达到高峰（Ｐ＜ ０．０１）;ＣＫ于１２小时最先达到衰竭标准（Ｐ＜ ０．０１）;ＡＬＴ与Ｃｒ均在２４ 小时后达到衰竭标准（Ｐ＜ ０．０５）。同时ＳＯＤ、ＧＳＨ Ｐｘ活性降低和ＭＤＡ含量增多（Ｐ均＜ ０．０５）。ＭＤＡ与ＣＫ、ＡＬＴ和Ｃｒ之间呈正相关关系,ｒ值分别为０．８４７、０．６３４和０．５６７（Ｐ均＜ ０．０１）。结论：在去甲斑蝥素致大鼠ＭＯＤＳ的病程中,心功能衰竭出现在先,然后为肾和肝功能衰竭。器官功能受损与氧自由基有关。     

冠心病患者脂质过氧化反应与血液流变学变化的研究

测定了３０例冠心病（ＣＨＤ）患者和３０例正常人血浆丙二醛（ＭＤＡ）含量、红细胞超氧化物歧化酶（ＳＯＤ）、全血谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）活力和部分血液流变学指标。结果表明，ＣＨＤ心绞痛发作期机体抗氧化能力明显降低，自由基增多及血液粘度增大，提示自由基损伤及脂质过氧化和高血粘度。Ｓｖａｔｅ有抗脂质过氧化及降低血浆纤维蛋白原的作用。     

冠心病患者血液流变学改变及其机理探讨

40例冠心病患者（心绞痛30例，ＡＭＩ１０例）１１项血液流变学指标观察发现：心绞痛患者全血低切粘度、红细胞聚集指数、纤健蛋白原、Casson屈服值明显升高，AMI患者相同指标有更明显的改变趋势，Casson屈服值是更灵敏的反映血液流变特性的指标。相关分析表明：血浆脂质过氧化物含量与纤维蛋白原合量呈明显正相关（r＝0．356，P＜0．05）。提示冠心病时脂质过氧化增强可能是致纤维蛋白原升高，血粘度升高的最终原因之一。     

Single dose administration of L-carnitine improves antioxidant activities in healthy subjects

L-carnitine has been used as a supplement to treat cardiovascular or liver disease. However, there has been little information about the effect of L-carnitine on anti-oxidation capability in healthy human subjects. This study was designed to investigate the correlation between plasma L-carnitine concentration and antioxidant activity. Liquid L-carnitine (2.0 g) was administered orally as a single dose in 12 healthy subjects. Plasma concentration of L-carnitine was detected by HPLC. The baseline concentration of L-carnitine was 39.14 ± 5.65 μmol/L. After single oral administration, the maximum plasma concentration (C(max)) and area under the curve (AUC(0-∞)) were 84.7 ± 25.2 μmol/L and 2,676.4 ± 708.3 μmol/L·h, respectively. The half-life and the time required to reach the C(max) was 60.3 ± 15.0 min and 3.4 ± 0.46 h, respectively. There was a gradual increase in plasma concentrations of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase and total antioxidative capacity (T-AOC) in the first 3.5 h following L-carnitine administration. The plasma concentrations of SOD, GSH-Px, catalase and T-AOC returned to baseline levels within 24 h. A positive correlation was found between L-carnitine concentration and the antioxidant index of SOD (r = 0.992, P < 0.01), GSH-Px (r = 0.932, P < 0.01), catalase (r = 0.972, P < 0.01) or T-AOC (r = 0.934, P < 0.01). In conclusion, L-carnitine increases activities of antioxidant enzymes and the total antioxidant capacity in healthy subjects. It may be useful as a supplementary therapy for chronic illnesses involving excessive oxidative stress.     

多发性骨髓瘤患者抗氧化酶活性与血清铜、锌、锰和硒含量的关系

动态地测定了多发性骨髓瘤(multiple myeloma,MM)缓解期和非缓解期病人内源性抗氧化酶体系的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)的活性,并同期测定了病人血清铜(Cu)、锌(Zn)、锰(Mn)和硒(Se)的含量。结果发现,MM非缓解期病人SOD、GSH-Px及CAT活性较正常对照组明显降低,血清Zn、Mn和Se含量明显降低,血清Cu含量显著升高。缓解期病人抗氧化酶活性及血清Cu、Zn、Mn和Se含量接近正常水平。SOD活性与血清Zn和Se含量呈正相关(r_(Zn)=0.746,P<0.001;r_(Se)=0.533,P<0.01),GSH-Px活性与血清Se含量呈显著正相关(r=0.822,P<0.005),CAT活性与血清Zn和Se含量呈正相关(r_(zn)=0.507,P<0.05,r_(Se)=0.694,P<0.001)。本研究结果对解释MM患者抗氧化酶活性降低的原因及指导临床治疗具有实际意义。     

Plasma total antioxidant capacity, lipid peroxidation, and erythrocyte antioxidant enzyme activities in patients with rheumatoid arthritis and osteoarthritis

OBJECTIVES: The metabolism of cells in inflammatory and noninflammatory arthritic joint diseases is subject to complex environmental controls. The aim of the present study was to investigate the total antioxidant capacity (TAC), levels of lipid peroxidation (LPO), and antioxidant enzyme activities in patients with rheumatoid arthritis (RA) and osteoarthritis (OA). DESIGN AND METHODS: Plasma levels of TAC, malondialdehyde (MDA), the activities of some erythrocyte antioxidant enzymes, as well as erythrocyte sedimentation rates (ESR) were estimated in patients with RA and OA and compared with controls. RESULTS: The plasma TAC levels were significantly lower in the RA group than the OA and control group (P < 0.05). Plasma MDA concentrations were significantly higher in patients with RA than those with OA and healthy subjects (P < 0.05). Erythrocyte GSH-Px and CAT activities were found to be significantly lower in patients with RA than those with OA and healthy subjects (P < 0.05, P < 0.05, respectively). However, there were no significant differences in erythrocyte SOD activities between the groups (P > 0.05). ESR were significantly higher in RA patients than in healthy subjects and patients with OA (P < 0.01). Moreover, there were significant negative correlations between TAC vs. MDA, ESR vs. TAC, and a positive correlation between ESR vs. MDA in the RA group (r = -0.398, P < 0.05; r = -0.422, P < 0.05; r = 0.530, P < 0.05, respectively). CONCLUSIONS: Our results demonstrated that levels of LPO are increased in patients with RA compared to patients with OA. In addition, plasma TAC levels are decreased in RA due to its inflammatory character. We conclude that detecting plasma TAC levels with this novel method may be used as a routine and rapid test to verify the levels of oxidative stress in RA. Furthermore, correlating TAC and LPO levels with acute phase reactants such as ESR may give some clues about disease activity in RA.     

血小板GMP-140、vWF、SOD、LPO与高血压病关系的研究

为了解高血压病（EH）发病机理，采用ELISA法和比色法检测EH患者血小板a-颗粒膜蛋白140（GMP－140）、血浆VonWillebrand因子（vWF）、超氧化物歧化酶（SOD）、过氧化脂质（LPO）的含量。结果显示EH患者血小板GMP－140、vWF和LPO水平均高于正常对照组（P＜0．01～0．001），SOD水平低于正常对照组（P＜0．01）。相关分析：GMP－140与vWF、LPO是正相关（r=0．78、0．82，P＜0．05）。表明：EH患者血小板活化与血管内皮细胞受损及自由基损伤关系密切。     

类风湿关节炎患者血浆对氧磷酯酶-1活性与氧自由基代谢水平的相关性研究

目的探讨类风湿关节炎（RA）患者血浆对氧磷酯酶-1（PON-1）活性与氧自由基代谢水平的关系。方法检测118例RA患者和56名健康对照者血浆PON-1活性、氧化修饰低密度脂蛋白（OX-LDL）、丙二醛（MDA）含量、超氧化物歧化酶（SOD）和循环谷胱苷肽过氧化物酶（GSH-Px）活性及晚期蛋白质氧化产物（AOPP）水平,分析PON-1活性与氧自由基代谢水平的关系。结果RA患者血浆PON-1、SOD、GSH-Px活性分别为（122．24-24．1）kU／L、（78．2±21．3）kU／L、（156．4±32．2）U／L,低于对照组（P〈0．01）。OX-LDL、AOPP、MDA水平分别为（832．0±256．2）μg／L、（342．3±118．1）μmol／L、（16．2±6．4）μmol／L,高于对照组（P〈0．01）。RA患者血浆PON-1活性与GSH-Px、SOD呈正相关（r：0．781,P〈0．01;r=0．702,P〈0．01）,与OX-LDL、MDA、AOPP呈负相关（r=-0．721．P〈0．01;r=-0．789,P〈0．01;r=-0．679,P〈0．01）。结论RA患者血浆PON-1活性降低,PON-1可能通过降低抗氧化能力机制参与了RA的病理生理过程。     

便秘型肠易激综合征患者的血浆5-HT水平与氧化应激作用的相关性分析

目的分析便秘型肠易激综合征患者的血浆5-羟色胺(5-HT)表达与氧化应激作用的相关性。方法选择78例便秘型肠易激综合征患者作为观察组,78例健康人作为对照组,两组均进行血浆5-HT、脂质过氧化物(LPO)和丙二醛(MDA)的检测,以及空腹血糖(FBG)、血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)的检测与相关性分析。结果观察组的血浆MDA浓度(4.46±0.51)nmol/L高于对照组(1.68±0.38)nmol/L,差异有统计学意义(P0.05);观察组LPO浓度(18.67±1.48)nmol/L高于对照组(6.62±1.29)nmol/L,差异有统计学意义(P0.05)。观察组血浆5-HT浓度(1 828.44±300.87)pg/mL高于对照组(1 236.93±289.48)pg/mL,差异有统计学意义(P0.05)。偏相关分析便秘型肠易激综合征的血浆5-HT与MDA(r=0.325,P0.05)、LPO(r=0.432,P0.05)、TC(r=0.357,P0.05)都呈正相关,而与HDL-C(r=-0.385,P0.05)呈负相关;多因素Logistic回归分析显示MDA、LPO、TC与HDL-C都为影响5-HT的独立危险因素(P0.05)。结论便秘型肠易激综合征患者中血浆5-HT呈现高表达状况,同时伴随有MDA与LPO的高表达,且MDA与LPO的表达情况会影响5-HT的表达,可能是便秘型肠易激综合征的病理生理机制之一。     

Blood oxidative stress status in patients with macrophagic myofasciitis.

The study aimed at determining the presence of an oxidative stress in patients with macrophagic myofasciitis (MMF), a new inflammatory myopathy with suspected toxic etiology related to aluminium hydroxide-containing vaccines. A total of 30 MMF patients (nine males, 21 females; aged 42+/-14 years), whose diagnosis was confirmed by deltoid biopsy, have been included and compared to 38 sex- and age-matched healthy control subjects (10 males, 28 females; aged 43+/-8 years). The blood oxidative stress status has been evaluated by assaying six parameters: plasma lipid peroxidation products (thiobarbituric acid-reactive substances: TBARS) and antioxidant defense systems: plasma vitamin E and glutathione peroxidase (GSH-Px) activity, erythrocyte GSH-Px and Cu,Zn-superoxide dismutase (SOD) activities. Plasma selenium was also determined as a trace element essential to the activity of GSH-Px. Statistical significance was evaluated by the Mann-Whitney test. Plasma GSH-Px activity, selenium and vitamin E concentration were significantly lower in MMF group than in controls (P=0.004, P=0.003 and P=0.009, respectively), with a positive correlation in MMF patients between plasma GSH-Px activity and selenium concentration (rho=0.0001). The other parameters of oxidative stress did not significantly differ between both groups. A macrophage activation could occur in MMF, consequently to chronic stimulation by aluminium-containing vaccines, and could participate to the lower values of selenium and vitamin E observed in comparison with controls. Nevertheless, since no deficiency in these elements has been observed, no supplementation is to be considered.     

不同粒径纳米和常规微米SiO_2对大鼠肺、肝、心、睾丸组织的氧化损伤作用

目的探讨不同粒径纳米和常规微米SiO2对大鼠肺、肝、心、睾丸组织的氧化损伤作用。方法采用气管直接滴注法给大鼠染毒,滴注后48 h处死大鼠,测定肺、肝、心、睾丸组织超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性及脂质过氧化作用(LPO)水平。结果①在67.5 mg/kg剂量下,20 nm SiO2、60 nm SiO2均可引起肺、肝、睾丸组织SOD活性显著降低(P0.05),微米SiO2只引起肺组织SOD酶活性降低(P0.05)。各实验组大鼠心脏SOD活性无显著性变化(P0.05)。②在67.5 mg/kg剂量下,20 nm SiO2、60 nm SiO2及微米SiO2均可引起肺、肝脏GSH-Px活性显著降低(P0.05),且20 nm SiO2还引起心肌组织GSH-Px活性显著降低(P0.05),微米SiO2只引起肺组织GSH-Px活性降低(P0.05)。③20 nm SiO2、60 nm SiO2均可引起肺、肝和睾丸组织LPO水平显著升高(P0.05),且20 nm SiO2还引起心肌组织LPO水平显著升高(P0.05),微米SiO2只引起肺组织LPO水平显著升高(P0.05)。结论微米SiO2只引起肺组织的氧化损伤,而20 nm SiO2、60 nm SiO2均可引起肺、肝、心、睾丸组织不同程度的氧化损伤作用,且20 nm SiO2的毒性作用强于60 nm SiO2的毒性作用。