CyclinD_1、CDK_4蛋白在食管癌中的表达及意义

目的为探讨ＣｙｃｌｉｎＤ１、ＣＤＫ４基因及蛋白在食管癌发生中的意义及其相互间的作用。方法采用ＬＳＡＢ免疫组化染色法,对４３例食管癌手术标本的ＣｙｃｌｉｎＤ１、ＣＤＫ４蛋白进行标记。结果在鳞癌组织中,两种抗体的阳性物质存在于细胞核中和／或胞浆中,在正常鳞状上皮主要存在于核中,且从正常鳞状上皮→癌旁不典型增生→鳞癌组织,ＣｙｃｌｉｎＤ１、ＣＤＫ４的阳性率逐渐上升,统计学处理：正常上皮与癌组织之间的阳性率有差异显著性（ＰＣｙｃｌｉｎＤ１＜０．０５,ＰＣＤＫ４＜０．０５）;但未检出ＣｙｃｌｉｎＤ１、ＣＤＫ４与癌分化的关系。另外,在正常鳞状上皮中,ＣｙｃｌｉｎＤ１、ＣＤＫ４则多集中在基底层的增殖带。结论ＣｙｃｌｉｎＤ１、ＣＤＫ４蛋白表达的变化是食管癌发生中的早期事件,并且在正常细胞周期中起正调控作用。     

Cyclin D1、P53在口腔鳞癌中的表达及意义

目的：研究ＣｙｃｌｉｎＤ１、Ｐ５３蛋白在口腔鳞癌中的表达及与口腔鳞癌发生、发展间的关系。方法：应用免疫组化ＳＰ法,检测了４０例口腔鳞癌中ＣｙｃｌｉｎＤ１、Ｐ５３蛋白的表达情况。结果：５７．５％（２３／４０）的口腔鳞癌ＣｙｃｌｉｎＤ１过表达。正常口腔粘膜Ｐ５３蛋白均为阴性;５２．５％（２１／４０）的口腔鳞癌Ｐ５３蛋白阳性,两组间差异有显著性（Ｐ＜０．０５）。其中高分化鳞癌阳性率为３６．８％（７／１９）;中分化鳞癌阳性率为６６．７％（１４／２１）,差异具有显著性（Ｐ＜０．０５）。结论：ＣｙｃｌｉｎＤ１过表达及Ｐ５３蛋白异常协同作用引起口腔鳞癌细胞的失控性生长,在口腔鳞癌的发生、发展中发挥着重要的作用。     

原癌基因蛋白CyclinD1在喉癌中的表达

目的：探讨ＣｙｃｌｉｎＤ１基因表达与喉癌发生发展的关系。方法：应用Ｓ－Ｐ免疫组化方法检测１０例声带息肉组织及４４例喉鳞癌组织中ＣｙｃｌｉｎＤ１蛋白的表达。结果：ＣｙｃｌｉｎＤ１在喉癌组织中的阳性表达率为６８．３％（３０／４４），在声带息肉组织中的阳性率为３０．０％（３／１０），是者差别有统计学意义，Ｐ〈０．０５，而在癌旁正常组织中未见表达。ＣｙｃｌｉｎＤ１蛋白表达与喉癌患者年龄、淋巴结合转移无明显     

细胞周期素D1表达对肾母细胞瘤生长的影响

目的：研究细胞周期素 Ｄ１ （ｃｙｃｌｉｎ Ｄ１）表达对肾母细胞瘤的影响。方法：应用原位杂交技术检测 ２３例肾母细胞瘤 ｃｙｃｌｉｎ Ｄ１ 表达情况。结果：１５ 例检测到ｃｙｃｌｉｎ Ｄ１ 表达阳性细胞,表达指数 ５６％ ～４３９％ 。ｃｙｃｌｉｎ Ｄ１ 表达阳性组瘤体重量显著大于阴性组（ Ｐ＜ ００５）;ｃｙｃｌｉｎ Ｄ１ 高表达者瘤体重量明显大于低表达者（ Ｐ＜ ００５）。临床晚期标本ｃｙｃｌｉｎ Ｄ１ 表达指数明显高于早期标本（ Ｐ＜ ００５）;不同组织类型标本 ｃｙｃｌｉｎ Ｄ１ 表达指数无显著性差异（ Ｐ＞ ００５）。ｃｙｃｌｉｎ Ｄ１ 表达指数与瘤体重量呈正相关（ｒ＝ ０５３, Ｐ＜ ００５）。结论：ｃｙｃｌｉｎ Ｄ１表达与肾母细胞瘤发生发展关系密切;过度表达时,肾母细胞瘤细胞增殖周期演进快,肿瘤生长迅速,预后不良。     

CyclinD1在子宫内膜癌中的表达及其意义

应用免疫组织化学技术（ＳＰ法），检测ＣｙｃｌｉｎＤ１基因在３６例子宫内膜癌中的表达。ＣｙｃｌｉｎＤ１在子宫内膜癌过表达阳性率为４１６７％（１５／３６），其表达与子宫内膜癌的临床分期，细胞分级、转移及患者预后有关（Ｐ＜００５）。结果提示，测定子宫内膜癌ＣｙｃｌｉｎＤ１蛋白有助于确定患者的临床分期，组织分化程度，判断肿瘤转移及估计患者的预后     

胃粘膜癌变过程中P1 6、CyclinD1的表达

目的：探讨Ｐ１６、ＣｙｃｌｉｎＤ１的表达在胃癌发生发展中的作用。方法：采用免疫组化Ｓ－Ｐ法。结果：在胃粘膜癌变过程中，Ｐ１６阳性表达率逐渐下降，其中异型增生和胃癌组Ｐ１６阳性表达率明显低于慢性浅表性胃炎组（Ｐ　＜０．０５）；ＣｙｃｌｉｎＤ１阳性表达率逐渐增高，其中肠化生、异型增生和胃癌组ＣｙｃｌｉｎＤ１阳性表达率明显高于慢性浅表性胃炎组（Ｐ　＜０．０５）。在胃癌中，Ｐ１６阳性表达率与胃癌分期和分化程度有关，ＣｙｃｌｉｎＤ１的阳性表达率与之无关。结论：Ｐ１６表达缺失和ＣｙｃｌｉｎＤ１异常表达是胃癌发生过程中一个较早的分子事件，且Ｐ１６表达缺失与胃癌进展和恶性程度有关。     

AR与cyclinD1、p16在喉鳞癌发病中作用及其相互关系

目的：探讨雄激素受体（ＡＲ）与细胞周期蛋白Ｄ１（ｃｙｃｌｉｎＤ１）、ｐ１６基因三者在喉鳞癌中的作用及相互关系。　方法：　应用ＡＲ、ｃｙｃｌｉｎＤ１、ｐ１６单克隆抗体，通过免疫组化Ｓ－Ｐ法检测４４例喉鳞癌和１１例癌旁组织中的ＡＲ、ｃｙｃｌｉｎＤ１、ｐ１６的表达。　结果：　ＡＲ、ｃｙｃｌｉｎＤ１、ｐ１６在喉鳞癌上皮组织中阳性表达分别是６８．２％，５６．８％，５２．３％，与癌旁正常组织对照，两者差异有显著性（Ｐ　＜０．０５），ＡＲ与ｃｙｃｌｉｎＤ１的表达呈正相关，同样ｃｙｃｌｉｎＤ１与ｐ１６的表达呈正相关，而ＡＲ与ｐ１６之间无相关性，同时发现ＡＲ、ｃｙｃｌｉｎＤ１、ｐ１６的阳性表达与临床及病理分级无相关性（Ｐ　＞０．０５）。结论：ＡＲ、ｃｙｃｌｉｎＤ１、ｐ１６蛋白阳性表达在喉鳞癌的发生和发展中起重要作用。ＡＲ在喉鳞癌上皮细胞的阳性表达可作为诊断喉鳞癌的辅助指标。     

喉癌癌变过程中CyclinD1表达及临床病理学意义

目的 探讨喉癌癌变过程中ＣｙｃｌｉｎＤ１表达的临床病理学意义。方法 用免疫组化检测２０例正常粘膜、４０例不典型增生病变和６０例喉癌组织中ＣｙｃｌｉｎＤ１的表达。结果 ＣｙｃｌｉｎＤ１阳性表达定位于细胞核。在喉癌变过程中,喉正常粘膜、不典型增生病变和喉癌中ＣｙｃｌｉｎＤ１阳性表达率分别为５．０％、３０．０％、５３．３％（Ｐ〈０．００１）。淋巴结转移级ＣｙｃｌｉｎＤ１阳性表达率明显高于非淋巴结转移给（     

细胞周期素D1在结肠癌中的表达

目的 探讨细胞周期素（ｃｙｃｌｉｎ）Ｄ１与ｐ５３基因在结肠癌中的表达意义及其关系。方法 应用原位杂交技术和免疫组织化学技术检测结肠癌中ｃｙｃｌｉｎ Ｄ１基因ｍＲＮＡ和ｐ５３蛋白的表达。统计处理采用卡方检验。结果 发现结肠癌组织和癌旁组织中ｃｙｃｌｉｎ Ｄ１表达阳性率分别为６３．４％（４０／６３）及４４．４４％（２８／６３）,两者之间差异有显著性（Ｐ〈０．０５）。ｃｙｃｌｉｎ Ｄ１表达程度与癌组织分化程度及临床分期有关,差异有显著性（Ｐ〈０．０５）。ｃｙｃｌｉｎ Ｄ１表达与其他临床病理指标无关（Ｐ〉０．０５）。ｐ５３蛋白在结肠癌中阳性率为３８．０９％（２４／６３）,ｐ５３阳性的患者其ｃｙｃｌｉｎ Ｄ１的表达较之ｐ５３阴性者为高,差异有显著性（Ｐ〈０．０５）。结论 ｃｙｃｌｉｎＤ１的过表达及ｐ５３突变在结肠癌的癌变     

食管鳞癌细胞周期调控基因CyclinD1蛋白和增殖细胞核抗原的表达

目的：探讨ＣｙｃｌｉｎＤ１和增殖细胞核怕（ＰＣＮＡ）在食管鳞癌,癌旁正常上皮和一上皮的表达特点,并分析了ＣｙｃｌｉｎＤ１在食管鳞癌发生,发展中的作用及与临床病理特点的关系。方法：应用免疫组织化学Ｓ－Ｐ法对８２例管鳞癌,３３例非典型增生上皮和２３例正常粘膜上皮进行了ＣｙｃｌｉｎＤ１和ＰＣＮＡ检测。结果：８２例食管鳞癌中５７例阳阳性表达,阳性率为６９．５％,非典型增生组阳性率为３６．４％,正常粘膜上皮     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.