担子菌黄卷缘齿菌的化学成分

目的研究担子菌黄卷缘齿菌Hydnum rep andum的化学成分。方法通过硅胶、Sephadex LH-20、反相硅胶柱色谱分离化合物,运用氢谱、碳谱、二维核磁共振(1H1-HCO SY,HM QC,HM BC,NOESY)、质谱、高分辨质谱、旋光鉴定结果。结果共分离鉴定了11个化合物,分别是:sarcodon in A(Ⅰ)、scabron ine B(Ⅱ)、3β-羟基-5,α8α-过氧麦角甾-6,22-二烯-3β-醇(Ⅲ)、(22E,24R)-麦角甾-7,22-二烯-3,β5,α6β-三醇(Ⅳ)、(22E,24R)-麦角甾-7,22-二烯-3β-醇(Ⅴ)、苯甲酸(Ⅵ)、对羟基苯甲醛(Ⅶ)、对羟基苯甲酸乙酯(Ⅷ)、乙基-β-D-吡喃葡萄糖苷(Ⅸ)、硫代乙酸酐(Ⅹ)、(2S,2′R,3S,4R)-2-(2-羟基-十八碳酰胺)二十二碳烷-1,3,4-三醇(Ⅺ)。结论所有化合物都是首次在黄卷缘齿菌中分到。     

中国南海黑乳海参共附生白色侧齿霉菌中的甾体类成分

目的对中国南海黑乳海参共附生白色侧齿霉菌Engyodontium album的次生代谢产物进行研究。方法运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等分离手段对白色侧齿霉菌Engyodontium album的乙酸乙酯提取物进行分离纯化,根据现代波谱技术结合文献报道进行结构鉴定。结果共分离得到5个化合物,分别鉴定为:(22E,24R)-5α,8α-过氧化麦角甾-6,22-二烯-3β-醇(1)、(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(2)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(3)、3β,5α-二羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(4)、3β,5α-二羟基-6β-甲氧基-麦角甾-7,22-二烯(5)。结论本研究是对黑乳海参共附生白色侧齿霉菌次生代谢产物的首次报道,这5个化合物均为首次从白色侧齿霉菌Engyodontium album中分离得到。     

大白口蘑子实体的化学成分研究

目的研究大白口蘑(Tricholoma giganteum Massee)子实体的化学成分。方法大白口蘑子实体的乙酸乙酯部位经过硅胶、RP-18、Sephadex LH-20等多种材料进行分离纯化,通过现代波谱技术进行结构鉴定。结果分离鉴定了12个化合物,它们分别为:亚油酸甲酯(1)、亚油酸(2)、麦角甾-4,6,8(14),22-四烯-3-酮(3)、麦角甾-5,7,22-三烯-3β-醇(4)、过氧麦角甾醇(5)、麦角甾-7,22-二烯-3β,5α,9α-三羟基-6酮(6)、麦角甾-7,22-二烯-3β,5α-二羟基-6酮(7)、麦角甾-7,22-二烯-3β,5α,6α,9α-四醇(8)、麦角甾-7,22-二烯-3β,5α,6β,9α-四醇(9)、麦角甾-7,22-二烯-3β,5α,6β-三醇(10)、3β-O-glucopyranosyl-5α,6β-dihydroxy-ergosta-7,22-diene(11)、脑苷脂D(12)。结论化合物1-3、6-12均为首次从该种真菌中分离得到。     

中国南海小月柳珊瑚Menella kanisa中过氧化甾体成分研究

目的研究中国南海小月柳珊瑚Menella kanisa中的化学成分。方法应用正相硅胶、Sephadex LH-20凝胶、半制备反相高效液相色谱法(RP-HPLC)等多种分离手段对小月柳珊瑚Menella kanisa的乙醚提取物进行分离纯化,根据现代波谱技术结合文献报道进行结构鉴定。结果和结论从南海小月柳珊瑚Menella kanisa中分离得到5个过氧化麦角甾,分别鉴定为:5α,8α-过氧化胆甾-6-烯-3β-醇(1)、(22E)-5α,8α-过氧化胆甾-6,22-二烯-3β-醇(2)、(22E,24R)-5α,8α-过氧化麦角甾-6,22-二烯-3β-醇(3)、5α,8α-过氧化麦角甾-6,24(28)-二烯-3β-醇(4)、24(E)-5α,8α-过氧化胆甾-24-乙基-6,24(28)-二烯-3β-醇(5)。这5个过氧化甾醇均为首次从该种珊瑚中分离得到。     

中国南海紫柳珊瑚中过氧化甾醇类化学成分的研究

目的对采自中国南海的紫柳珊瑚(Muriceopsis flavida)的生物活性成分进行研究。方法应用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、反相高效液相层析(RP-HPLC)等分离手段对紫柳珊瑚的乙醚提取物进行分离纯化,应用核磁共振(NMR)、质谱(MS)等波谱手段对得到的化合物进行结构鉴定,采用琼脂扩散试验法对分离得到的化合物进行体外抗微生物活性测试。结果从紫柳珊瑚乙醚提取物中分离得到5种过氧化甾醇,分别鉴定为:(22E,24S)-5α,8α-过氧化麦角甾-6,22-二烯-3β-醇(1)、(22E,24R)-5α,8α-过氧化麦角甾-6,22-二烯-3β-醇(2)、(24R)-5α,8α-过氧化胆甾-24-乙基-6-烯-3β-醇(3)、(22E)-5α,8α-过氧化胆甾-6,22-二烯-3β-醇(4)、5α,8α-过氧化胆甾-6-烯-3β-醇(5)。体外抗微生物活性测试表明这5种化合物均有不同程度的抗微生物活性。结论首次从中国南海紫柳珊瑚中得到5种过氧化甾醇,其中化合物2对微藻显示强烈的生长抑制活性,具有进一步研究的价值。     

花生茎叶化学成分研究(Ⅱ)

目的 研究花生茎叶的化学成分.方法 采用硅胶柱色谱和凝胶柱色谱Sephadex LH-20进行分离纯化,通过理化方法和波谱数据分析进行结构鉴定.结果 从70%乙醇提取物中分离并鉴定了11个化合物,分别为β-胡萝卜苷(Ⅰ)、正二十六碳酸乙酯(Ⅱ)、棕榈酸(Ⅲ)、5,8-过氧化麦角甾-7,22-二烯-3β醇(Ⅳ)、水杨酸(Ⅴ)、尿嘧啶核苷(Ⅵ)、(22E,24R)-麦角甾-7,22-二烯-3β醇(Ⅶ)、2-O-甲基-肌醇(Ⅶ)、9(Z),12(Z)-十八二烯酸(Ⅸ)、麦角甾-5,22-二烯-3-醇-7-酮(Ⅹ)、正二十九烷(Ⅺ).结论 除化合物Ⅰ、Ⅲ,其余化合物均为首次从该属植物中分得.     

变绿红菇化学成分研究

目的研究变绿红菇Russula virescens子实体化学成分。方法变绿红菇子实体用95%乙醇浸泡提取浓缩,经硅胶、RP-8、Sephadex LH-20等多种材料进行分离,波谱学方法鉴定化合物的结构。结果分离鉴定了8个化合物,分别为铁屎米酮(canthin-6-one,Ⅰ)、3β-羟基-5α,8α-过氧化麦角甾-6,22-二烯(Ⅱ)、3β-羟基-麦角甾-5,7-22-三烯(Ⅲ)、3β,5α,6β-三羟基-麦角甾-7,22-二烯(Ⅳ)、硫代乙酸酐(Ⅴ)、顺丁烯二酸(Ⅵ)、D-阿洛醇(Ⅶ)、腺嘌呤核苷(Ⅷ)。结论所有化合物均为首次从该菌中分到,其中化合物为首次从该属中分到。     

茯苓化学成分分离与结构鉴定

目的 研究茯苓中化学成分,阐明茯苓药效物质基础.方法 采用稀乙醇提取,各种柱色谱法如硅胶、反相、凝胶等,以及结晶法进行分离纯化,用紫外光谱、核磁共振、质谱等各种光谱波谱技术对分离得到的化合物进行结构鉴定.结果 从茯苓中分离得到10个化合物,分别鉴定为乙酰依布里酸(1),麦角甾醇过氧化物(2),麦角甾-7,22-二烯-3β,5α,6β-三醇(3),麦角甾-7-烯-3β醇(4),麦角甾-7,22-二烯-3β醇(5),茯苓酸(6),去氢土莫酸(7),3-表-去氢土莫酸(8),依布里酸(9),3β-羟基-羊毛甾-7,9(11),24-三烯-21-酸(10).结论 化合物1、2、3、4为首次从茯苓中分离得到.     

块花柳珊瑚化学成分的研究

目的对采自中国广西省北海附近的块花柳珊瑚(Anthogorgia sp.)进行化学成分的研究。方法用硅胶柱层析(石油醚:丙酮=99:1,49:1,39:1,34:1,29:1,24:1,19:1,14:1,11:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,100%丙酮;氯仿:甲醇=24:1,19:1,14:1,9:1,7:1,5:1,3:1,1:1,1:4,1:9,1:39,100%甲醇)梯度洗脱和SephadexLH-20凝胶柱层析(正己烷:氯仿:甲醇2:1:1洗脱)对块花柳珊瑚丙酮提取物的乙醚部分进行分离纯化,并利用经1HNMR、13CNMR、MS等现代光谱技术对其进行结构鉴定。结果分离得到6个甾醇类化合物以及1个神经酰胺,其结构分别为:麦角甾-5,24(28)-二烯-3β-醇(1)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(2)、(22E,24S)-麦角甾-5,22-二烯-3β-醇(3)、胆甾-5-烯-3β,7β,19α-三醇(4)、(22E)-胆甾-5,22-二烯-3β-醇(5)、胆甾醇(6)、N-正十六碳酰基-正十八碳-4(E)-烯鞘胺醇(7)。结论这些化合物均首次从该种海洋动物中分离得到。     

旱生卷柏化学成分研究(Ⅲ)

卷柏科植物旱生卷柏Selaginella stauntoniana Spring为多年生草本,分布于华北、西北等地,民间药用全草,具有活血散瘀、凉血止血的功效[1]。本实验从旱生卷柏醇提取物低极性部位中首次分离得到5种成分,经理化、TLC和光谱分析确定结构为:豆甾-4-烯-3,6-二酮()、β-谷甾醇()、5α,8β-环二氧麦角甾-6,22二烯-3β醇()、大黄酸()、芦荟大黄素(),其中化合物、、、为卷柏科乃至蕨类植物中首次分离得到。1材料与仪器旱生卷柏由中国药品生物制品检定所张继副主任药师等采于河北省雾灵山大沟村,并鉴定学名;薄层色谱硅胶G和柱色谱硅胶(青岛海洋化工…     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.     

Dr.Zhang Ren's Experience in the Acupuncture Treatment of Different Diseases with the Same Therapeutic Principle

Dr.Zhang Ren,the chief physician,is the chairman of Shanghai Acupuncture and Moxibustion Association.Having been engaged in medicine for about 40 years,he is experienced in treating various intractable diseases.In his long years of clinical practice,he advocates taking the TCM differentiation as the basis to seek for the acupuncture method for treatment of modern intractable diseases.The author of this essay had the fortune to follow Dr.Zhang in study.The following is a summary of Dr.Zhang's experience in the acupuncture treatment for different intractable diseases with the same therapeutic principle.     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.