子宫内膜异位症合并不孕者生殖内分泌的改变

目的 通过检测血液及腹腔液中生殖内分泌激素水平,了解内分泌因素在子宫内膜异位症所致不孕中的作用。方法 运用荧光微粒子发光法（MEIA）,于卵泡期对78例不孕或子宫内膜异位症（EM）患（EM合并不孕32例,单纯EM20例,单纯不孕26例）及14名健康绝育的血液及腹腔液进行生殖内分泌激素的检测。结果 EM血中催乳素（PRL）、雌二醇（E2)及睾酮（T）水平高于无EM（P＜0．05）,EM合并不孕PRL及T高于EM非不孕;重度EM的PRL、E2及T高于中度和轻度EM（P＜0．05）;无EM的不孕除LH较正常对照组高外,其余与正常对照组之间无差异;卵泡刺激素（FSH）及孕酮（P）水平在以上各组之间无差异（P＞0．05）。EM合并不孕组腹腔液中PRL、LH、E2及T水平高于其他各组（P＜0．05）,而FSH及P与其他各组之间无差异（P＞0．05）。EM非不孕PRL高于非EM不孕及正常对照组（P＜0．05）、LH则与正常对照组无差异,但低于非EM不孕组（P＜0．05）,而FSH、E2及P与非EM不孕及正常对照组（P＜0．05）、LH则与正常对照组无差异（P＞0．05）;非EM不孕组与正常对照组之间除LH较高外,其余各项无差异;不同程度EM组之间除FSH无差异外,其余各项均有差异,重度高于中度,中度又高于轻度EM（P＜0．05）。结论 子宫内膜异位症特别是合并不孕血及腹腔液中PRL和T水平较高,这可能是EM导致不孕的原因之一。     

性激素与前列腺癌病理分级及临床分期的相关性

目的  探讨前列腺癌（PCa）患者血清性激素睾酮（T）、雌二醇（E2）、孕激素（P）、卵泡刺激素（FSH）、黄体生成素（LH）及泌乳素（PRL）的水平及其与PCa的病理分级及临床分期的相关性。方法  PCa和良性前列腺增生症（BPH）患者各45例,另选取体检健康男性45例为正常对照组。采用电化学发光免疫分析法测定性激素六项（T、E2、P、FSH、LH及PRL）的水平。对PCa患者进行病理分级及临床分期,分析组间差异。评估各检测指标与PCa的病理分级及临床分期的相关性。结果  与BPH组及正常对照组比较,PCa组患者血清T降低、FSH和LH增高,差异有统计学意义（P <0.05）,E2、P、PRL比较,差异均无统计意义（P >0.05）;而BPH组患者T、E2、P、FSH、LH及PRL水平与正常对照组比较,差异无统计学意义（P >0.05）。相关性分析结果显示,PCa患者血清T、E2、P、FSH、LH及PRL水平与PCa的病理分级及临床分期均无相关性（P >0.05）。结论  血清T、FSH、LH水平对前列腺良、恶性疾病的鉴别有指导意义。PCa患者血清各项性激素水平与PCa病理分级及临床分期无相关性。

     

子宫内膜异位症合并不孕妇女内分泌的临床研究

目的探讨子宫内膜异位症合并不孕女性内分泌及自身免疫抗体情况。方法分别抽取子宫内膜异位症患者(A组)、不孕症患者(B组)及子宫内膜异位症合并不孕患者(C组)各60例为研究对象,同时选取60例健康妇女作为对照组,分别检测其血清中相关内分泌及自身免疫抗体情况。结果 C组E2、INS、T、PRL水平显著高于其他三组(P0.05);A组血清PRL水平显著高于B组及对照组(P0.05)。不同程度子宫内膜异位症患者血清LH、FSH水平之间无差异(P0.05);三组血清E2、T、PRL、INS均存在差异,差异有统计学意义(P0.05),且重度组各指标均高于中、轻度组,中度组各指标高于轻度组,差异有统计学意义(P0.05)。A、B、C三组在ASAb、AOAb、EMAb、ACAb、HCGAb及总抗体阳性反应率方面高于对照组(P0.05);C组在各抗体及总抗体阳性反应率方面显著高于A、B组(P0.05);A组及B组在各抗体及总抗体阳性率方面无差异(P0.05)。结论子宫内膜异位症合并不孕患者常有E2、INS、T、PRL内分泌异常,且伴有免疫系统紊乱。临床通过内分泌及免疫手段可能对此类患者的临床诊治提供指导依据。     

探讨子宫内膜异位症(EMS)合并不孕患者血清内分泌激素水平及自身免疫抗体情况

目的探讨子宫内膜异位症(EMS)合并不孕患者血清内分泌激素水平及自身免疫抗体情况;方法选择我院2013年1月至2015年1月收治的子宫内膜异位症及不孕症患者100例,按照患者疾病类型将其分成单纯不孕组(A组)30例、单纯子宫内膜异位症组(B组)35例、子宫内膜异位症合并不孕症组(C组)35例,另选择35例健康体检者当成对照组。全部患者均给予化学免疫荧光法测定和ELISA酶联合免疫法测定;结果 C组患者的PRL、T、INS、E2水平显著高于A组、B组(P0.05);三组患者的LH、FSH水平比较差异无统计学意义(P0.05);A组、对照组患者的血清PRL水平显著低于B组患者(P0.05),其他指标比较差异无统计学意义(P0.05);C组的抗体总阳性率显著高于其他各组(P0.05);A、B两组患者的抗体阳性率比较差异无统计学意义(P0.05)。结论子宫内膜异位免疫失衡导致的T、PRL、E2内分泌异常是导致子宫内膜异位症合并不孕症的主要因素,分析和研究患者的内分泌水平,能为临床诊治提供科学依据。     

增龄与成年男性垂体性腺激素水平关系

目的探讨增龄对成年男性垂体性腺激素水平的影响.方法将80名成年男性分为三个年龄组-青年组、中年组、老年组,用放射免疫分析法(RIA)测定其血液中垂体性腺激素水平.结果睾酮(T)随年龄增加而明显降低,青年组>中年组>老年组(P<0.01).FSH随年龄增加明显升高,各组间差异非常显著(P<0.01).LH同时随之升高,但差异并不明显(P>0.05).各组之LH/FSH为青年组3.3,中年组2.0,老年组1.1.各组的PRL值均无明显差异(P>0.05).结论随增龄发生功能改变的原发部位在性腺而不在垂体,发生原发性分泌减少的部位为性器官,男性为睾丸.     

子宫内膜异位症合并不孕妇女内分泌临床研究

目的：探析子宫内膜异位症合并不孕女方内分泌激素水平及自身抗体免疫情况。方法选取该院2012年1月—2014年12月收治的120例子宫内膜异位症(EMS)及不孕症患者,并根据患者疾病类型分为单纯不孕组(A组)38例、单纯子宫内膜异位症组(B组)42例及子宫内膜异位合并不孕症组(AB组)40例,同时选取健康体检者40例,作为对照组,采取化学免疫荧光法测定各组患者血清中的T(睾酮)、INS(胰岛素)、E2(雌二醇)、PRL(泌乳素)、FSH(卵泡刺激素)及LH(黄体生成素)水平,并采取ELISA酶联合免疫法进行测定各组患者AOAb(抗卵巢抗体)、ACAb(抗心磷脂抗体)、ASAb(抗精子抗体)、HCGAb(抗绒毛促性腺激素抗体)及EMAb(抗子宫内膜抗体)阳性情况。结果 AB组患者的PRL、INS、T、E2水平均明显高于A组和B组,差异有统计学意义(P<0.05),FSH及LH水平3组差异无统计学意义(P>0.05),A组和对照组患者的血清PRL水平显著低于B组,差异有统计学意义(P<0.05),其他各指标差异无统计学意义(P>0.05);AB组的抗体总阳性率明显高于其他3组,差异有统计学意义(P<0.05),A组和B组之间抗体阳性率差异无统计学意义(P>0.05)。结论引起患者发生子宫内膜异位症不孕症的主要因素有可能是子宫内膜异位免疫失衡引起的T、E2、和PRL内分泌异常,通过对其内分泌水平进行分析研究,旨在为临床提供可靠依据。     

血管生成因子VEGP和bFGF与子宫内膜异位症的相关性研究

目的检测血管内皮生长因子(VEGF)和碱性成纤维细胞 生长因子(bFGF)在子宫内膜异位症(EM)中的表达,探讨两种因子与EM发病机制的关系.方法TSS〗收集EM患者的子宫内膜异位病灶组织35例,在位内膜组织38例及非子宫内膜异位症患者的子宫内膜37例,采用免疫组织化学SP法分别测定VEGF及bFGF在内膜组织中的表达情况,并用组织学评分对实验结果进行半定量统计,比较其表达强度.结果①VEGF在三组内膜的腺体及间质细胞中均有表达,主要定位于细胞浆.异位内膜组的表达强度高于正常对照组,二者差异有显著性(p<0.05).在位内膜组高于正常对照组,差异有显著性(P<0.05).异位内膜组同在位内膜 组比较,差异无显著性(P>0.05);②EM患者的VEGF在异位内 膜组和正常对照组均无周期性变化,而在EM患者的在位内膜中VEGF的表达强度分泌期比增生期明显增强,在月经周期中呈周期性变化;③bFGF在正常子宫内膜及EM患者在位内膜和异位内膜的增生期和分泌期中均有表达,表达强度差异无显著性(P>0.05),无周期性变化;④VEGF和bFGF在EM患者增生期和分泌期的在位内膜和异位内膜中的表达呈正相关关系.结论VEGF和bFGF均为促进血管生成的重要细胞因子,在子宫内膜异位症的异位内膜及在位内膜均有表达,从而表明VEGF和bFGF的促血管生成是EM发生发展中的一个重要环节.     

男性肺癌患者血清性激素水平的分析

目的探讨男性肺癌患者血清性激素水平的变化及临床意义。方法检测27例肺癌患者、19例肺良性病变患者和34例正常健康人员的血清卵泡刺激素(FSH)、黄体生成素(LH)、雌二醇(E2)、睾酮(T)、孕酮(P)及催乳素(PRL),并进行统计学分析。结果肺癌患者血清T水平较正常对照组显著性降低(P<0.01),E2较正常对照组显著性增高(P<0.01),而P、LH、PRL及FSH较正常对照组无显著性差异(P>0.05);肺良性病变组血清T、P、E2、LH、PRL及FSH水平较正常对照组均无显著性差异(P>0.05)。结论男性肺癌患者体内存在性激素水平的失衡和紊乱,检测血清中T和E2的水平对肺癌的诊断和病情变化监测具有一定价值。     

子宫肌瘤患者及接受UAE治疗后血清性激素水平分析

目的：探讨性激素在子宫肌瘤发病中的意义。方法：采用化学发光技术检测正常对照61例(对照组)、子宫肌瘤患6l例(患组)以及44例子宫肌瘤接受子宫动脉栓塞治疗(UAE)后的患，血清促卯泡成熟激素(FSH)、促黄体生成激素(LH)、雌激素(E2)、垂体泌乳素(PRL)、睾酮(T)水平。结果：子宫肌瘤患组的血清FSH、LH、PRL、T水平均明显高于对照组；血清E2水平与对照组无显差异。其中44例接受UAE治疗的子宫肌瘤患术后血清LH、E2、PRL、T水平均比术前明显降低，FSH与术前无显差异；术后血清FSH、PRL、T水平均明显高于对照组，LH、E2水平明显低于对照组。结论：PRL、T是子宫肌瘤的发病因素。     

子宫内膜异位症合并不孕妇女内分泌临床研究

目的:研究分析子宫内膜异位合并不孕症患者的内分泌情况.方法:根据2012年1月至2014年12月我院的120例子宫内膜异位症(EMS)合并不孕症患者来进行研究分析,这些患者分组为A组单纯不孕症患者共38例,B组单纯子宫内膜异位症患者共42例,AB组子宫内膜异位症合并不孕症患者共40例,对照组选取同期健康体检者40例,采取化学免疫荧光法测定各组患者血清中的睾酮(T)、胰岛素(INS)、雌二醇(E2)、泌乳素(PRL)、卵泡刺激素(FSH)及黄体生成素(LH)水平,并采取ELISA酶联合免疫法进行测定各组患者抗卵巢抗体(AOAb)、抗心磷脂抗体(ACAb)、抗精子抗体(ASAb)、抗绒毛促性腺激素抗体(HCGAb)及抗子宫内膜抗体(EMAb)阳性情况.结果:PRL、INS、T、E2水平为AB组比A和B组高,结果存在差异(P<0.05),FSH及LH水平三组对比无差异(P>0.05),血清PRL为A组和对照组患者较低,B组较高,结果存在统计学差异(P<0.05),其他指标无统计学差异(P>0.05).结论:子宫内膜异位症合并不孕症患者临床中主要的发病因素是子宫内膜异位导致的T、E2、和PRL内分泌异常,临床中对患者的内分泌情况进行研究分析能够为治疗提供参考.     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

安心颗粒对急诊经皮冠状动脉介入术后生活质量影响的研究

目的：评价使用安心颗粒对急诊经皮冠状动脉介入术（PPCI）术后生活质量的影响.方法：将160例接受PPCI的急性ST段抬高型心肌梗死患者随机分为安心颗粒组（术前顿服安心颗粒8.8g,术后安心颗粒4.4 g/次,每日2次）和对照组（仅接受基础药物治疗）.所有患者均服用阿司匹林、氯吡格雷和阿托伐他汀.分别在入院时、出院前1d、出院后180 d时,应用心肌梗死多维度量表（MIDAS）、中文版SF-36评价量表对患者生活质量评分.并观察术后30 d以内的出血并发症、血小板减少症发生情况.结果：入院时和出院前1d,两组患者的心肌梗死MIDAS、SF-36量表评分比较无差异（P＞0.05）;出院后180 d时,与对照组比较,安心颗粒组MIDAS、SF-36评分明显减低（P＜0.05）;组内与入院时比较,两组出院前1d、出院后180 d时,MIDAS、SF-36评分均降低（P＜0.05）.两组患者在随访期间均无大量出血、少量出血、重度和极重度血小板减少症发生,安心颗粒组有4例、对照组有7例发生不明显出血（P＞0.05）.两组发生轻度血小板减少症的患者数比较无差异（P＞0.05）.结论：PPCI使用安心颗粒,能改善急性ST段抬高型心肌梗死患者的生活质量,且不增加出血风险.     

The comparative studies of the influences of Urapidil and Nicardipine on sino-atrial node function, atrio-ventricular node function and hemodynamics

Objective:To investigate the influences of urapidil and nicardipine on rabbit sinus function,atrio-ventricular node function and hemodynamics.Methods:Thirty-two Angora's rabbits were selected and randomly divided into four groups.U1 group:urapidil 0.25 mg/kg;U2 group:urapidil 0.5 mg/kg;N1 group:nicardipine 10 μg/kg;N2 group:nicardipine 20 μg/kg.All these medicine were administrated within 30 seconds.Measurements were taken before and after the administration of urapidil or nicardipine for the following data:mean blood pressure(MAP),heart rate(HR),sino-atrial conduction time(SACT),maximal sinoatrial recovery time(SNRTmax)corrected sinus node recovery time(CSNRT),index of sinus node recovery time(SNRTI),Wenckebach A-V conduction frequency (WB),and P-R interval.Results:Significant MAP and HR changes were identified in all of the four groups before and after administration of both urapidil and nicardipine.No significant changes could be found in the rest of the parameters.Intergroup analysis showed that SACT and CSNRT of N1 and N2 groups were shorter than those of the U2 group(P＜0.01);the MAP decreased(P＜0.01)and the HR increased drastically(P＜0.01).Conclusions:Neither urapidil(0.25 mg/kg,0.5 mg/kg)nor nicardipine(10μg/kg,20μg/kg)has any significant influence on rabbit sinus function or rabbit atrio-ventricular node function.Nicardipine could be a better choice than urapidil for parafunctional sinus node patients.     

Expression of OPGmRNA and ODFmRNA in the patients of hip fracture due to osteoporosis

Objective：To investigate the gene expression of osteoprotegerin（OPG） and osteoclast differentiation factor（ODF） in the bone tissue of patients with hip fracture due to osteoporosis. Methods：OPGmRNA and ODFmRNA in the bone tissue in 50 cases of osteoporosis sufferers（over 50 years old） with hip fracture（Observer Group） and 30 cases of hip facture sufferers with no osteoporosis（Control group） were analyzed with the Semi-Quantitative RT-PCR method. Results：The mRNA expressed of ODF, OPG were both high in the patients with hip fracture. In the control group, the expression of OPG mRNA was observed, while the expression of ODF mRNA was very slight. Conclusion：Aged patients contained all signals including OPG, ODF that are essential for inducing osteoclastogenesis and promoting bone resorption.     

Dynamic Changes in Serum Estradiol and Progesterone levels in Patients of Premenstrual Syndrome with Adverse Flow of Liver-qi

Objective:To probe into the influence of changes of ovarian hormones on the pathogenesis of the specific sub-type premenstrual syndrome(PMS)and reveal partial microcosmic mechanisms of adverse flow of liver-qi.Methods:Estradiol(E2)and progesterone(P)levels in serum were determined at different phases of menstrual cycle by radioimmunoassay.Results:In the group of PMS with adverse flow of liver-qi.the secretive peak value Of E2 and P at the follicular phase significantly decreased,and the secretive peak value at the luteal phase did not come into being.Conclusions:Low E2 and P secretive peak at the follicular phase and absence of secretive peak at the luteal phase is one of the microcosmic mechanisms of PMS with adverse flow of liver-qi.One of the pathophysiologic mechanisms of specific sub-type PMS is probably the continuous low level of E2and P.     

Real-time Three-dimensional Echocardiography in Assessment of Left Ventricular and Right Ventricular Volumes

Real-time three-dimensional echocardiography (RT3DE)is a new ultrasound technique that enables dynamic threedimensional visualization and quantification of the heart in real time. Investigation of feasibility and methodology of RT3DE in determining left ventricular (LV) and right ventricular (RV) volumes, RT3DE was performed in 35 normal adults using Philips SONOS 7500 system with a 2-4 MHz matrix array transducer. The 60°×60° "pyramid" volume database was obtained and analyzed on a TomTec echo workstation. Both LV and RV volumes were calculated with four 3DE methods (i.e. apical 2, 4, 8, and 16-plane) through manually tracing ventricular endocardial borders in end diastole and end systole. Stroke volumes were then calculated. LV volume was also measured by 2DE Simpson's rule using GE VIVID 7 ultrasound machine.     

SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN

Increasing maternal age is the only etiological factor unequivocally linked to Down's syndrome in humans. The occurrence rate of newborns with Down's syndrome is about 1/220 in women over 35 years old. However, the occurrence rate in embryos fertilized in vitro, of the elder woman is unclear. Using FISH we screened the number of chromosome 21 in preimplanted embryos of 5 elderly women (average age, 38.4 years) to study the feasibility and necessity of screening trisomy 21 in embryos in patients over 35 years old at the in vitro fertilization (IVF) center.