c-myc反义寡核苷酸对低氧内皮细胞条件培养液诱导的肺血管周细胞增殖的影响

应用细胞培养、核酸斑点杂交、~３Ｈ－胸腺嘧啶核苷（~３Ｈ－ＴｄＲ）掺入、免疫细胞化学、图像分析等技术观察ｃ－ｍｙｃ反义寡核苷酸（ＯＤＮｓ）对低氧内皮细胞条件培养液（ＨＥＣＣＭ）诱导的大鼠肺血管周细胞ｃ－ｍｙｃ ｍＲＮＡ表达、增殖细胞核抗原（ＰＣＮＡ）表达及~３Ｈ－ＴｄＲ掺入量的影响。结果表明,ＨＥＣＣＭ显著促进周细胞 ｃ－ｍｙｃ ｍＲＮＡ表达、ＰＣＮＡ表达（Ｐ＜０．０１）及~３Ｈ－ＴｄＲ掺入量增加（Ｐ＜０．０１）,反义ＯＤＮｓ显著阻抑 ＨＥＣＣＭ诱导的周细胞ｃ－ｍｙｃ ｍＲＮＡ表达、ＰＣＮＡ表达（Ｐ＜０．０１）及~３Ｈ－ＴｄＲ掺入量增加（Ｐ＜０．０５）,而同义ＯＤＮｓ无上述作用（Ｐ＞０．０５）。结果提示,ＨＥＣＣＭ可通过上调ｃ－ｍｙｃ基因表达促进肺血管周细胞增殖,反义ＯＤＮｓ通过下凋ｃ－ｍｙｃ基因表达,抑制ＨＥＣＣＭ诱导的肺血管周细胞增殖。     

大肠癌中bcl-2和增殖细胞核抗原的表达及其临床意义

目的　研究大肠癌中ｂｃｌ－２和ＰＣＮＡ的表达及其临床意义,探讨其细胞凋亡和细胞增殖之间的关系。方法运用免疫组化法ＳＰ法检测５６例大肠癌组织中ｂｃｌ－２和ＰＣＮＡ的表达。结果５６例大肠癌中ｂｃｌ－２和ＰＣＮＡ的阳性率分别为６０．７％和１００．０％。ｂｃｌ－２在侵及肌层和Ａ、Ｂ期的表达高于侵及浆膜及浆膜外和Ｃ、Ｄ期（Ｐ＜０．０５）,高、中分化癌高于低、未分化癌但无明显差异（Ｐ＞０．０５）;ＰＣＮＡ增殖指数在低、未分化癌,侵及浆膜及浆膜外和Ｃ、Ｄ期高于高、中分化癌,侵及肌层和Ａ、Ｂ期（Ｐ＜０．０１）;ｂｃｌ－２和ＰＣＮＡ的表达无相关关系（Ｐ＞０．０５）。结论大肠癌中ｂｃｌ－２的表达与浸润深度和Ｄｕｋｅｓ分期密切相关,而与分化程度无关,ＰＣＮＡ增殖指数与肿瘤分化程度、浸润深度和Ｄｕｋｅｓ分期有关。     

p53,p21及—c—erbB—2蛋白在大肠粘膜和肿瘤中的表达

目的探讨ｐ５３、ｐ２１、及ｃ－ｅｒｂＢ－２蛋白与大肠癌发生和发展的关系。方法运用免疫组化方法检测ｐ５３、ｐ２１、及ｃ－ｅｒｂＢ－２蛋白在６８例大肠癌、１１例大肠腺瘤、２６例癌旁粘膜及１５例正常大肠粘膜中的表达情况。结果①正常粘膜及癌旁粘膜中无ｐ５３蛋白过度表达。大肠癌ｐ５３蛋白表达率比腺瘤高（Ｐ＜０．０５）。正常粘膜ｐ２１及ｃ－ｅｒｂＢ－２蛋白的阳性表达率比癌、腺瘤及癌旁粘膜低（Ｐ＜０．０１或Ｐ＜０．０５）。②正常粘膜以３种蛋白全部阴性者为多，癌旁粘膜以１种蛋白阳性或全阴者为多。③ｐ５３及ｃ－ｅｒｂＢ－２蛋白阳性的大肠癌和腺瘤的ＰＣＮＡ（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）增殖指数高（Ｐ＜０．０１或Ｐ＜０．０５），而ｐ２１蛋白阳性及阴性的大肠癌和腺瘤的ＰＣＮＡ指数无明显差别（Ｐ＞０．０５）。ｐ２１及ｃ－ｅｒｂＢ－２蛋白阳性对癌旁粘膜及正常粘膜的ＰＣＮＡ增殖指数无明显影响。结论癌基因和抑癌基因蛋白协同表达可能与大肠腺瘤和癌的发生有关，尤其与ｐ５３蛋白的关系较为密切，似通过导致细胞过度增殖而使肿瘤发生和恶性转化。     

放疗前、中c-myc蛋白表达与宫颈癌细胞凋亡和增殖的关系

目的：探讨在放射治疗前、中宫颈癌细胞ｃ－ｍｙｃ基因表达与细胞凋亡和增殖的关系。方法：对 ４８例宫颈癌患者分别于放疗前、放疗 ２０Ｇｙ／２周取活检,石蜡包埋。应用原位ＤＮＡ缺口未端标记法（ＴＵＮＥＬ）检测细胞凋亡,应用免疫组化法对ｃ－ｍｙｃ蛋白、增殖细胞核抗原（ＰＣＮＡ）进行检测。结果。 ４８例宫颈癌患者放疗前、放疗 ２０Ｇｙ凋亡阳性率分别为６７％、８５％,有显著性差异（Ｐ＜０．０５）。放疗前ｃ－ｍｙｃ蛋白阳性表达率为 ７８％,ＰＣＮＡ阳性表达率为８６％,放疗２０Ｇｙ其表达率分别为４３％和５５％,放疗前、中比较均有显著性差异（Ｐ＜０．０１）。放疗２０Ｇｙ ｃ－ｍｙｃ蛋白表达与凋亡有明显相关性。放疗前及放疗２０Ｇｙ ｃ－ｍｙｃ蛋白表达与ＰＣＮＡ表达有相关性。结论：ｃ－ｍｙｃ蛋白表达与宫颈癌细胞增殖有关,亦与放射诱导宫颈癌细胞凋亡有关。     

鼻咽癌P^53,C—myc,EGFR基因表达的研究

目的：Ｐ＾５３，Ｃ－ｍｙｃ及ＥＧＦＲ基因在鼻咽癌（ＮＰＣ）的发生、发展中可起重要作用，基因的研究结果可能为ＮＰＣ的预防及诊疗提供科学的依据。方法：用免疫组化Ｓ－Ｐ法检测了３０例ＮＰＣ组织、１０例无瘤鼻咽癌（ＮＰ）组织的Ｐ＾５３、Ｃｍｙｃ及ＥＧＦＲ基因的异常表达情况。结果：①三种基因在ＮＰＣ组织中均有较高水平的表达，Ｐ＾５３蛋白阳性率为８６．７％（２６／３０），Ｃ－ｍｙｃ和ＥＧＦＲ蛋白阳性率均为７０     

ＲＮＡｉ沉默ＰＥＬＰＩ／ＭＮＡＲ基因对子宫内膜癌细胞增殖和细胞周期的作用

【目的】 构建脯氨酸－谷氨酸－亮氨酸富集蛋白１（ＰＥＬＰ１）基因慢病毒表达载体,研究沉默ＰＥＬＰ１／雌激素受体非基因组活性辅助调节因子（ＰＥＬＰ１／ＭＮＡＲ）基因表达对子宫内膜癌细胞增殖和周期的作用及机制。【方法】 构建合成靶向ＰＥＬＰ１／ＭＮＡＲ ＲＮＡｉ慢病毒表达载体,并转染子宫内膜癌Ｉｓｈｉｋａｗａ细胞,Ｒｅａｌ－ｔｉｍｅ ＰＣＲ和ｗｅｓｔｅｒｎ ｂｌｏｔ检测ＰＥＬＰ１／ＭＮＡＲ ｍＲＮＡ和蛋白水平的表达。使用普通培养基和加入Ｅ２的培养基培养各组细胞,ＭＴＴ检测各组细胞增殖情况;流式细胞仪检测细胞生长周期情况;Ｗｅｓｔｅｒｎ ｂｌｏｔ检测ＥＲ下游靶基因ｃ－ｆｏｓ,ｃｙｃｌｉｎ Ｄ１的蛋白表达水平。【结果】 成功构建合成靶向ＰＥＬＰ１／ＭＮＡＲ ＲＮＡｉ慢病毒表达载体,转染子宫内膜癌Ｉｓｈｉｋａｗａ细胞,转染后 ＰＥＬＰ１／ＭＮＡＲ ｍＲＮＡ的表达和蛋白的表达分别下降８６％和６５％（Ｐ ＜ ０．０５）。转染后Ｉｓｈｉｋａｗａ细胞与对照组相比增殖抑制（Ｐ ＜ ０．０５）, 细胞周期Ｇ０／Ｇ１期细胞比例增加,Ｓ期细胞比例减少 （Ｐ ＜ ０．０５）。加入雌激素后,三组细胞的生长速度加快,细胞Ｓ期的比例增加,转染组增殖抑制明显（Ｐ ＜ ０．０５）,Ｓ期比例降低（Ｐ ＜ ０．０５）。转染组ＥＲ下游基因ｃ－ｆｏｓ、ｃｙｃｌｉｎ－Ｄ１蛋白水平均显著降低（Ｐ ＜ ０．０５）。【结论】 在子宫内膜癌细胞中沉默ＰＥＬＰ１／ＭＮＡＲ的表达能能抑制细胞生长、使细胞阻滞在Ｇ１期,下调ＥＲ靶基因蛋白表达,ＰＥＬＰ１／ＭＮＡＲ有望成为子宫内膜癌治疗的潜在靶点。     

鼻咽癌的癌基因与抗癌基因癌蛋白表达的研究

应用免疫组化方法检测了５８例鼻咽癌和２４例鼻咽良性疾患的表皮生长因子受体（ＥＧＦＲ），Ｎ－ｒａｓ，ｃ－ｍｙｃ，ｃ－ｅｒｂＢ－２，Ｐ５３和增殖细胞核抗原（ＰＣＮＡ）的表达情况。结果发现鼻咽癌中ＥＧＦＲ，Ｎｒａｓ，ｃ－ｍｙｃ，Ｐ５３和ｃ－ｅｒｂＢ－２的阳性率分别为２９．３％，１７．２％，１２％，８．６％和１２％。鼻咽癌中ＰＣＮＡ的阳性率为１７．９１％，鼻咽良性疾患为６．６７％，两者有极显著的差别（ｔ＝     

EGF、EGFR及PCNA在肺癌中的表达

用Ｓ－Ｐ免疫组化方法对５２例原发性肺癌作ＥＧＦ、ＥＧＦＲ和ＰＣＮＡ检测。结果显示,４６例ＮＳＣＬＣ中ＥＧＦ、ＥＧＦＲ的阳性表达率分别为６０．９％和６３％,６例小细胞癌和正常肺组织无表达。有转移的肺癌组ＥＧＦＲ的阳性表达率高于无转移组（Ｐ〈０．０５）。５２例肺癌中ＰＣＮＡ的阳性表达率为７８．８％。ＮＳＣＬＣ中ＥＧＦ、ＥＧＦＲ的表达与肺癌分期、病理分级以及ＰＣＮＡ的表达呈正相关。以上结果提示ＥＧＦ、Ｅ     

血管内皮细胞生长因子及其受体在大肠癌组织中的表达

目的观察血管内皮生长因子（ＶＥＧＦ）及其受体（ＫＤＲ）在大肠癌组织中的表达情况。方法利用免疫组化ＳＡＢＣ 法检测８０例大肠癌组织及１０例正常大肠组织中的ＶＥＧＦ及ＫＤＲ的表达。结果ＶＥＧＦ染色性物质主要位于肿瘤细胞 膜及胞浆,ＫＤＲ染色性物质主要位于癌组织及癌组织旁的血管内皮细胞上,大肠癌组织中ＶＥＧＦ和ＫＤＲ的表达阳性 率显著高于正常对照组织（Ｐ＜０．０１）,ＶＥＧＦ和ＫＤＲ的表达与肿瘤的分化程度及Ｄｕｋｅｓ分期密切相关（Ｐ＜０．０５）,与病理 分型无关（Ｐ＞０．０５）。结论ＶＥＧＦ和ＫＤＲ在大肠癌的发生和发展中起着重要作用,可反映大肠癌的恶性程度和进展情 况,并作为预后的指标。     

相近分化的肺鳞癌和腺癌中PCNA及EGFR表达的研究

探讨ＰＣＮＡ和ＥＧＦＲ在肺鳞癌、腺癌中的表达及与癌组织分型，淋巴结转移等方面的关系。方法：用免疫组化Ｓ－Ｐ法分别对４４分分化相近肺癌组织、１６例正常肺组织ＰＣＮＡ和ＥＧＦＲ的表达进行检测。结果：肺癌组织ＰＣＮＡ和ＥＧＦＲ的表达明显高于正常肺组织。鳞癌与腺癌比较，ＰＣＮＡ和ＥＧＦＲ表达无统计学差异。（Ｐ〉０．０５）。淋巴结转移阳性癌组织ＰＣＮＡ和ＥＧＦＲ表达与淋巴 结转移阴性癌的组织比较，无学差异（     

Value of D-Dimers in patients with acute aortic dissection

Objective: To evaluatel the value of D-dimers in patients with acute aortic dissection (AAD). Methods: This study consisted of 16 patients with AAD and 27 non-AAD patients. Serum D-dimets were measured by Sta-Liatest D-DI immunoturbidimetric assay. Results: D-dimer level was higher (P ＜ 0.001) in patients with AAD(7.91 ± 5.52 μg/ml) than that in non- AAD group(1.57±1.24 μg/ml). D-dimer was positive (＞0.4 μg/ml) in all patients with AAD and in 10 control group patients (37%). Among patients with acute AAD, D-dimers tended to be higher in Stanford A than in Stanford B (8.67 ± 4.31 μg/ml vs. 3.24±1.27 μg/ml, P ＜0.01). D-dimer values tended to be higher in more extended disease(3.84 ± 1.65 μg/ml, 8.57 ± 3.58 μg/ml and 11.87 ± 5.69 μg/ml in thoracic aorta, thoracic and abdominal aorta, thoracic and abdominal aorta and iliacal arteries, respectively, P ＜ 0.05 for both 8.57 ± 3.58 and 11.87 ± 5.69 vs. 3.84 ± 1.65 ). Including the control group into the analysis, we found a sensitivity of 100%, a negative predictive value of 100%, and a specificity of 66% and a positive predictive value of 64% for D-dimer in diagnosis of AAD in our patients with suspected AAD. Conclusion: D-dimer was elevated in patients with AAD. A negative D-dimer test result could be useful in excluding AAD.     

Research of combined liver-kidney transplantation model in rats

Objective： To set up a simple and reliable rat model of combined liver-kidney transplantation. Methods： SD rats served as both donors and recipients. 4℃ sodium lactate Ringer＇s was infused from portal veins to donated livers,and from abdominal aorta to donated kidneys, respectively. Anastomosis of the portal vein and the inferior vena cava （IVC） inferior to the right kidney between the graft and the recipient was performed by a double cuff method, then the superior hepatic vena cava with suture. A patch of donated renal artery was anastomosed to the recipient abdominal aorta. The urethra and bile duct were reconstructed with a simple inside bracket. Results： Among 65 cases of combined liver-kidney transplantation, the success rate in the late 40 cases was 77.5%. The function of the grafted liver and kidney remained normal. Conclusion： This rat model of combined liver-kidney transplantation can be established in common laboratory conditions with high success rate and meet the needs of renal transplantation experiment.     

APPLICATION OF LORNOXICAM TO PATIENT-CONTROLLED ANALGESIA IN PATIENTS UNDERGOING ABDOMINAL SURGERIES

FOR anesthesiologis s ,treatingpostoperativepainhas alwaysbeen a problem.Althoughopioidshave been provedtobe effective,theirsideeffectscouldnotbeignored.With thedevelopmentofscienceand pharmacology,many drugs with aspectsof satisfactoryanalgesicefficacyand couldbe welltoleratedby patientshave been developed.And lornoxicamisone of them, which isa non-steroidalanti-inflammatorydrug (NSAID ), with analgesic, anti-infl-ammatory,andantipyreticproperties.Itseliminationhalf-time(3 to 5 hours) isle…     

BLOOD PRESSURE CHANGE WITH AGE IN SALT-SENSITIVE TEENAGERS

Objective To observe blood pressure change with age in salt-sensitive teenagers whose salt sensitivity were determined by repeated testing.Methods Salt sensitivity was determined through intravenous infusion of normal saline combined with volume-depletion by oral diuretic furosemide in 55 teenagers. After five years, salt sensitivity was re-examined and subject blood pressure was followed up. Blood pressure changes in salt-sensitive teenagers were compared to that of non-salt sensitive teenagers over five years.Results After 5 years, the repetition rate of salt sensitivity determined by intravenous saline loading is 92.7%. In teenagers with salt sensitivity on the baseline, both the systolic blood pressure increments and increment rates were much higher than non-salt sensitive teenagers (12.7±12.1 mmHg vs. 2.8±5.2 mmHg, P＜ 0.01; 12.2%± 12.0% vs. 2.5% ±4.4%, P＜ 0.001,respectively). There was a similar trend for diastolic blood pressure (8.4 ± 6.4 mmHg vs. 3.7 ± 6.4 mmHg, P = 0.052; 13.2% ±10.6 % vs. 6.8%± 10.1%, P = 0.053, respectively).Conclusions Salt sensitivity determined by intravenous saline loading showed good reproducibility. Blood pressure increments with age were much higher in salt-sensitive teenagers than non-salt sensitive teenagers, especially in terms of systolic blood pressure.     

Development on Adjustable Calibration Marker for Shock Wave Focus

Shock wave lithotripsy （SWL） is a treatment of choice for upper urinary stones. However, this procedure is inappropriate for obese patients because the focus is often unable to reach the target owing to the limited focal distance in shock wave source. Although treating such patients in a blast path may increase the application length of shock wave source, it＇s difficult to find this path on the lithotripter monitor. For this reason, we invented an adjustable calibration marker in order to set an effective focus in the shock wave hath.     

Study on the TCM Pathogenesis of Angiopathic Complications of Type II Diabetes

Excess production of reactive oxygen species(ROS)of mitochondrion mediated by hyperglycemia is the common pathogenesis of angiopathic complications of diabetes.TCM holds that the damp from the dysfunction of spleen.kidney and liver is the causative factor of complications of diabetes.This is similar to the mechanism of Ros resulting in angiopathic complications of diabetes.When the angiopathic complications of type II diabetes mellitus(T2DM)are difierentiated as caused by turbid damp in TCM can be explained as ROS.Since the obstruction of pathogenic damp in channels and collaterals is said to be the main pathogenesis,the treating principle should be dissolving the damp to remove the obstruction.     

Leptin, Ghrelin and TNF-α before and after Hypo-caloric Traditional Chinese Diet and Auricular Acupuncture

INTRODUCTION Obesity is a complex emergent problem, which can be possibly solved not only by the diet but also by the life style and promotion of a constant physical exercise. 1, 2 No doubt careful attentions must be given to the nutritional condition of obese people, the dietary habits, the somatic build (i.e. distribution of fat mass) and the organic functions linked to formation of the fat mass. All the parameters should be constantly monitored before, during and after a diet treatment. 3, 4, 5     

Relationship between Dysglycemia and Carotid Atherosclerosis in Tibetan Population

People with dysglycemia are at high risk for atherosclerotic diseases. This study aims at investigating the atherosclerotic vascular damage in dysglycemia and its metabolic origin in Tibetan population.     

Acupuncture Combined with Spinal Tui Na for Treatment of Primary Dysmenorrhea in 30 Cases

Objective: To observe the therapeutic effects in acupunture treatment of primary dysmenorrhea combined with spinal Tui Na, and study its mechanism. Methods: Thirty cases of the treatment group were treated by acupuncture combined with spinal Tui Na, and thirty cases in the control group were treated by routine acupuncture. Results: The total effective rate was 93.3% in the treatment group, and 73.3% in the control group, with a significant difference between the two groups (P<0.05). Conclusions: Acupuncture combined with spinal Tui Na has good prospects for treatment of primary dysmenorrhea.     

Luo Lingjie's Experience in Treating Chronic Nephropathy

In treating chronic nephropathy,Luo Lingjie,a chief physician,pays attention to regulating the balance between yin and yang,treating infection if present,and removing pathogenic factors.He prescribes gentle drugs and uses carefully strongly warming-tonifying ones,emphasizes the importance of persuading the patient to persist in treatment with medication and nurse one's health for recuperation,and is good at combined use of TCM and western medicine therapy and brings the merits of various therapies into full play,with obvious theraoeutic effects.