细胞培养分离人轮状病毒的研究

本文报道用MA104细胞从4名急性肠炎患儿的粪液标本中,分离出3株致细胞病变的轮状病毒。细胞病变特征为细胞边缘交模糊,细胞融合,堆积,变圆以及细胞瓦解。我们发现用细胞培养分离HRV不仅取决于接种和培养条件,而且很大程度上取决于粪液标本中病毒颗粒的结构。     

昆明地区婴幼儿腹泻中发现的星状病毒

用直接电镜和免疫电镜法,我们对1983年秋季昆明地区婴幼儿腹泻114名患者粪便作了检查,发现5例(4.4％)含有星状病毒。用免疫电镜测定,有2例双份血清中,有抗体4倍特异增长。星状病毒有光滑的边缘,呈圆形,直径28—30nm,表面呈5—6个星角及一个白的中心,两星角之间的表面凹陷,呈三角形。     

INFANTILE ROTAVIRUS ENTERITIS TREATED WITH HERBAL VALIRIANA JATAMANSI(VJ)

Valiriana Jatamansi(VJ),a Chinese herb wasfound effective against rotavirus enteritis in clinicalpractice.Animal experiments carried out by theInstitute of Medical Biology in Kunming have alsodemonstrated the efficacy of VJ.From December 22,1982 to January 4,1983,102 patients with rotavirus enteritis were admittedto our hospital.Of these 74 cases were randomlydivided into 3 groups:Group A(46 cases),GroupB(12 cases) and Group C(16 cases).In group A,the VJ-treated group,the drug was given orally ata dosage of 5 ml every 6 hrs for patients under 1year;10 ml every 6 hrs to patients over 1 year.Ingroup B,the control group,a solution of vitaminsBoo.and C was given orally.In group C,the West.ern medicine treated group,Gentamycin or SMZwas given in common dosage.Groups A and C werealso given the vitamins Bco.and C orally as in groupB.Patients with water and electrolyte disturbancein all groups received fluid therapy as indicated.Within 72 hours of medication the rate of cessa-tion of diarrhea in groups A,B and C were 73.92%,41.67% and 56.25% respectively.The rate in groupA was considerably higher than group B.Percentagesof patients whose temperature returned to normalafter treatment in groups A,B and C were 90.64%,60%and 92.30% respectively,suggesting antipyreticeffect of VJ.     

抗轮状病毒单克隆抗体简报

本研究用牛轮状病毒NCDV株为抗原,免疫2月龄Balb/c雌鼠,二个半月后取小鼠脾细胞与小鼠骨髓瘤细胞SP_2/o融合,共融合二次,融合率达100％。平行采用酶联免疫吸附试验和血凝抑制试验两种方法,从192个原始孔中初步筛选出12个阳性孔,其中11个孔为酶联免疫吸附试验阳性、1个孔为血凝抑制阳性。将其中几个孔进行三次有限稀释克隆化,得到3株抗轮状病毒单克隆抗体(2G_2、3F_4、4B_5)。在酶联免疫吸附试验中,2G_2株     

乙型肝炎病毒(HBV)感染树鼩的实验研究(摘要)

至今,除人类以外,对HBV敏感的动物只有黑猩猩和长臂猿。这些高等灵长类动物稀少珍贵,应用极其有限。树鼩(Tupaia)介于低等灵长类和啮齿类之间,易于捕获饲养。本文探讨树鼩对HBV是否易感,获得初步结果。材料和方法选用云南树鼩(Tupaia Belangeriyunalis)自野外捉来后笼养2周,健     

细胞培养分离人轮状病毒的研究

1980年Wyatt等报道,用含人轮状病毒(HRV)的粪液感染已知菌小猪,经体内传代后,接种于细胞培养获得第一株HRV—Wa株。最近,一些研究者报道,分离到HRV。     

秋季婴幼儿急性胃肠炎病源——轮状病毒的研究

秋季婴幼儿腹泻在我国流行多年,但病原问题长期未能解决。1973～1974年Bishop,1974 Flewett 发现了轮状病毒,此后世界上几个技术先进国家都先后发现了此病毒。本文报道1978年对北京两个医院收治的部份患儿所进行的病源学研究。     

急性胃肠炎病原之一——嵌杯病毒的研究

1982年,我们在北京从137例急性胃肠炎婴儿的粪中检出2例嵌杯病毒(Cali—civirus).本文在国内首次扼要报道对此病毒的形态学、血清学及其与疾病关系的研究结果.实验方法和结果(一)病毒形态学1.病毒大小:测量216个嵌杯病毒     

EXPERIMENTAL INFECTION OF ADULT TUPAIA BELANGERI YUNALIS WITH HUMAN ROTAVIRUS

This article deals with studies in late autumn 1981 0f the infection of wild adult Tupaia belangeri yunalis (a species of tree shrew caught in autumn in Yunnan province) with human rotavirus., 12 tupaias are infected in the first passage, 3 in the second passage and 3 in the third passage, 12/12, 2/3 and 2/3 0f the tu- paias develop diarrhea during the 3 passages. Electron microscopic observat.ion shoiws that 12/12, 3/3 and 2/3 0f the tupaias excrete rota- virus during the passages. Three tupaias died during the first passage and show mucosal ne- crosis and lymphocyte infiltration in sections of different intestinal parts. Immune electron mi- croscopically, anti-rotavirus antibody is assayed in sera of 2 infected tupaias, in both of which titer rises greater than 4 fo.lds in the convalescent phase serum as compared with the pre-infection serum. These results indicate that the adult tupaia is probably susceptible to human rota- virus.     

中草药“秋泻灵”实验治疗树鼩人工感染轮状病毒肠炎的研究

本文报道用中药“秋泻灵”治疗实验感染树鼩轮状病毒肠炎的结果,并与ORS治疗组和不治疗对照组进行了比较,用“秋泻灵”治疗的6只动物,平均止泻18小时,平均失重2.5克,全部治愈,ORS治疗的5只动物,平均止泻56小时,失重6.6克,其中1只死亡,未治疗对照组的5只动物平均止泻93小时,失重6.8克,其中3只死亡,4只死亡动物肠组织切片有粘膜坏死及淋巴细胞浸润,结果表明:“秋泻灵”是一种比ORS好的治疗轮状病毒肠炎的药物。