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北京市顺义县50岁及以上人群中盲患病率调查   总被引:79,自引:13,他引:66  
目的 调查北京市顺义县50岁及以上人群盲患病率,并与1985年调查结果相比较,以此评价近10余年该县实施防盲治项目的效果。方法 1996年9~11月采用整群随机抽样方法在顺义县抽取28个调查点,对50岁及以上人群进行视力和眼部检查。正式现场调查之前先进行预试验,并进行保证调查质量的重复性检验。结果 检录5555例中,受检人数为5084例,受检率为91.5%。以世界卫生组织视力损伤为标准,盲患病率为  相似文献
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Numerous animal studies indicate that aldose reductase inhibitors (ARIs) are beneficial for the prevention or amelioration of diabetic complications such as neuropathy, nephropathy and the ocular complications of cataract, retinopathy and keratopathy. To aid in the identification of novel potent ARIs, we have previously developed a screening method that is based on the formation of a non-covalent ternary tight-binding enzyme-inhibitor-nucleotide (AR-ARI-NADPH) complex that can be isolated using YM-10 filter units. Here, we report a modification of this method that permits us to rapidly identify tight binding ARIs that are isolated by denaturation from AR-ARI-NADPH complexes that are free of possible contamination resulting from the reaction of methanol with the YM-10 filter units. For the development of this procedure, nine structurally diverse ARIs were mixed with purified recombinant rat lens aldose reductase (RLAR) bound with NADPH to form tight-binding RLAR-ARI-NADPH complexes. These complexes were purified by high pressure Sephadex 75 size exclusion chromatography using ammonium acetate buffer and the formation of each complex was confirmed by electrospray ionisation mass spectrometry (ESI-MS). Each of the complexes was then denatured with methanol, rechromatographed on the size exclusion column, and the identity of the bound ARIs was confirmed by ESI-MS. The apparent ARI binding with aldose reductase to form a tight binding ARI complex appeared proportional to their IC50 values. This procedure allows for the rapid identification of tight binding ARIs with apparent IC50s<0.1 microm.  相似文献
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