Conditioned Medium from Human Umbilical Vein Endothelial Cells Promotes Proliferation,Migration, Invasion and Angiogenesis of Adipose Derived Stem Cells

Preeclampsia (PE) is a pregnancy-specific hypertensive complication, closely related to endothelial dysfunction. Adipose derived stem cells (ADSCs) have the capacity to differentiate into endothelial cells for vascular repair. Therefore, we hypothesized that induced endothelial differentiation of ADSCs might hold great potential for the treatment of PE. In this study, the primary ADSCs and human umbilical vein endothelial cells (HUVECs) were isolated by the collagenase digestion method. The supernatant of HUVECs was collected from the first generation of cells. Then, ADSCs were divided into two groups: ADSCs alone group and induced ADSCs (iADSCs) group. In iADSCs group, ADSCs were induced by HUVECs conditioned medium and ADSCs special culture medium at a ratio of 1:1 over a two-week period. In order to identify the endothelial characteristics of iADSCs, CD31 and CD34 were examined by flow cytometry. The proliferation, migration, invasion and angiogenesis assays were employed to compare the bioactivity of iADSCs and ADSCs. Furthermore, The levels of angiogenic related factors including vascular endothelial growth factor (VEGF) and placenta growth factor (P1GF) were detected by RT-PCR and Western blotting. Results showed conditioned medium from HUVECs promoted ADSCs proliferation, migration, invasion and angiogenesis. In addition, the levels of VEGF and P1GF were significantly enhanced in iADSCs group. This study uncovered the iADSCs application potential in the therapy and intervention of PE.     

“妊娠期微小病毒B19感染的临床实践指南”解读

正人微小病毒B19(简称B19病毒)是一种单链、线性DNA病毒,是学龄儿童传染性红斑(又称第五病)的病原体。妊娠期急性B19病毒感染的发生率约为1%~2%,在感染爆发流行时妊娠期妇女的感染率可上升至10%~([1])。由于B19病毒可以通过胎盘,孕妇感染B19病毒后可通过母胎垂直传播途径引起胎儿宫内感染。尽管大多数情况下胎儿宫内感染可自发缓解,不影响胎儿结局,但部分患者B19病毒宫内感染可导致胎儿水肿、死胎和自然流