基于“肝肾同源”理论探析椎动脉型颈椎病治疗思路

椎动脉型颈椎病(CSA)是常见病、多发病,因其患病率高、难以治愈的发病特点,严重影响患者的生活工作质量。通过对古代文献和现代研究的深入挖掘,探讨“肝肾同源”理论与CSA病因病机的联系,阐明“补肾益肝”法治疗CSA的临床疗效,探索有效的中医药防治方案,发挥中医药治疗CSA的独特优势以及提供相应的理论参考和治疗思路。     

经后路伤椎置钉结合功能锻炼治疗胸腰椎骨折临床观察

目的:探讨经伤椎椎弓根钉内固定结合术后功能锻炼治疗胸腰椎骨折的方法及效果。方法 :短节段经伤椎治疗胸腰椎骨折患者78例,72例获得随访,术后对伤椎前缘高度、伤椎Cobb角、椎管容积、腰背痛及神经功能恢复等方面进行评价。结果:获得随访的患者术后伤椎前缘高度恢复至(95.2±3.5)%,脊柱Cobb角恢复至术后的(4.5±3.2)°,伤椎平均正中矢状径恢复至术后的(96.5±2.3)%,手术前后有显著性差异(P0.05)。患者腰背痛症状明显缓解,VAS评分由术前的(9.4±0.6)分降到术后的(2.4±2.7)分。神经功能除术前4例A级的患者下肢感觉、运动无改善,剩下患者神经功能均有1~3级的恢复;术后随访出现1例患者椎弓根螺钉断裂现象。结论:经伤椎椎弓根钉内固定术对骨折复位满意,重建脊柱的稳定性,术后功能锻炼对腰背痛及术后神经功能恢复起到促进作用,减少术后并发症。     

杜仲诱导骨髓间充质干细胞成骨分化防治骨质疏松症相关信号通路研究进展

骨质疏松症（osteoporosis,OP）已成为全世界面临的重大公共卫生健康问题,尤其随着我国人口老龄化进程的加速,对其的防治已刻不容缓。近年来,随着对我国传统中医药的挖掘与探索,多项研究发现补肾中药杜仲对于骨质疏松症的防治效果明显。现代药理学研究表明,杜仲有效成分可以通过调节雌激素水平、骨代谢相关细胞因子及护骨素的表达对成骨细胞、破骨细胞、骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）增殖分化综合作用,有效防治骨质疏松。而BMSCs作为成骨细胞的前体细胞,探索其分化机制对于指导防治骨质疏松症具有重大意义。本文对传统中药杜仲诱导BMSCs向成骨细胞分化的相关信号通路作以综述,以期为OP的诊疗提供新思路。     

医科院校对生物化学实验教学改革的尝试与探索

通过调整生物化学实验的内容、改变教学模式、开放实验室等措施，对生物化学实验教学实施进行改革尝试。在加强学生基本操作训练、培养创新能力和科研意识等方面，收到了良好的效果，对培养适应社会需要的医学人才有积极意义。     

周期性牵张椎间盘纤维环细胞肌动蛋白骨架的重排

BACKGROUND: When the intervertebral disc is under stress, the hydraulic pressure generated inside the nucleus pulposus makes the annulus fibrosus extend outward and expand, and the annulus collagen fibers are stretched so that the extracellular matrix of annulus fibrosus cells is also under the pressure. In the intervertebral disc, aggrecan is the main component of proteoglycans, matrix metalloproteinase-2 is a major enzyme for extracellular matrix degradation, and tissue inhibitor of metalloproteinase is a multifunctional specific inhibition factor for matrix metalloproteinase activity. There is a mutual regulation between the latter two to keep the homeostasis between them. OBJECTIVE: To investigate the mechanism of cyclic tensile strain in the metabolism of intervertebral disc annulus matrix. METHODS: Rat anulus fibrosus cells were subjected to 2% or 10% cyclic tensile strain at 1.0 Hz for 2 and 12 hours using Flexcell4000 tension system. Then cells were collected and cultured in conditioned medium for gene and protein detection. Real-time quantitative PCR was used to detect mRNA expression of aggrecan, matrix metalloproteinases-2 and tissue inhibitor of metalloproteinase-2. Gelatin zymography was used to detect matrix metalloproteinases-2 activity. RESULTS AND CONCLUSION: The use of 2% cyclic tensile strain had no obvious effect on the stress fiber of actin cytoskeleton, whereas actin cytoskeleton was depolymerized in response to 10% cyclic tensile strain. The 2% cyclic tensile strain raised the expression of Aggrecan at 12 hours; whereas raised the matrix metalloproteinases-2 and tissue inhibitor of metalloproteinase-2 at 2 hours, both of which were in homeostasis; matrix metalloproteinases-2 activity had no significant changes. 10% cyclic tensile strain had no effect on the mRNA expression of Aggrecan. No matter stretching 2 or 12 hours, the matrix metalloproteinases-2 was up-regulated, and the tissue inhibitor of metalloproteinase-2 was down-regulated, both of which were not in balance. Moreover, the matrix metalloproteinases-2 activity was not significantly changed. These findings indicate that the mRNA expressions of Aggrecan, matrix metalloproteinases-2 and tissue inhibitor of metalloproteinase-2 alter in response to cyclic tensile strain in rat anulus fibrosus cells, and the tensile strain induces different mechano-responses in the actin cytoskeleton.      

新型C形椎管内骨折块复位器治疗胸腰椎骨折的临床研究

目的:评估自制C型椎管内骨折块复位器在胸腰椎骨折后路手术中的疗效及应用价值。方法:36例病人手术前CT及MRI提示胸腰椎骨折并伴有椎体后缘骨折块突入椎管压迫脊髓,行后路切开复位椎弓根钉棒系统内固定+全椎板减压,用自制C型椎管内骨折块复位器在后侧方推挤使椎体后缘骨折块复位,达到恢复椎管容积的目的,并自体骨横突间植骨,术后随访以评价脊柱矫形、椎管占位、复位及神经功能恢复情况(Frankel分级法)。结果:术后X光平片、CT检查36例椎管容积均恢复至正常80%以上,。术后随访6~72月(平均24月),未发生椎体后突畸形,Frankel分级平均进步1~2级。结论:自制C形椎管内骨折块复位器扩大了胸腰椎骨折后路手术治疗适应证范围,复位效果确切,操作简便安全,值得推广。     

肱骨髁间骨折的治疗近况

肱骨髁间骨折目前多倾向于开放复位内固定治疗,特别是对肱骨髁间粉碎性骨折,并强调手术时间越早越好.外固定时间要视内固定牢固性而定,一般不宜超过3周.     

脊柱调衡手法对腰神经后支卡压综合征的作用探讨

通过探讨腰神经后支卡压综合征的应用解剖、发病机理,脊柱调衡手法的操作要领、生物力学和治疗作用机制,认为脊柱调衡手法是治疗腰神经后支卡压综合征的一种安全有效的保守疗法.     

中药熥敷保健颈托的研制及临床应用

目的:研制一种治疗颈椎病的中药熥敷保健颈托,并观察其临床疗效.方法:中药熥敷保健颈托由普通颈托与中药熥敷合剂药袋组成,药袋粘贴入特制的颈托凹陷内.选择160例颈椎病患者随机分为观察组和对照组,观察组采用佩戴中药熥敷保健颈托治疗,对照组采用奇正炎痛贴治疗,2个疗程后观察疗效.结果:观察组治愈率和有效率分别为72.5%和9...     

后方入路经椎旁病灶清除椎弓根钉内固定治疗胸腰椎结核42例

目的：探讨后方入路经椎旁病灶清除、植骨、椎弓根钉内固定技术治疗胸、腰椎结核的疗效。方法：对42例胸腰椎结核患者采用后方入路经椎旁病灶清除、植骨、椎弓根钉内固定术,术后定期随访,观察近、远期疗效。结果：所有切口一期愈合,全部病例植骨骨性融合,神经损害有1～2级的改善,脊柱后凸畸形有不同程度纠正,结核无局部复发。结论：后方入路经椎旁病灶清除、植骨、椎弓根钉内固定术是治疗胸腰椎结核简便、有效、安全的方法。