牛脑提取液治疗大鼠脊髓损伤的实验研究

目的：探讨牛脑提取液对实验性大鼠脊髓损伤的作用饥制：方法：健康SD大鼠64只，随机分为硬膜内给牛脑提取液组、硬膜内给生理盐水组、腹腔内给牛脑提取液组、腹腔内给生理盐水组。采用改良的Allen氏装置制作模型：运用脊髓神经功能、体感诱发电位、辣根过氧化酶示踪、大体观察、组织学及超微结构变化作为观察指标进行实验观察。结果：实验组与对照组相比．其各项观测指标皆有明显改善；局部用药组观察指标明显优于全身用药组。结论：牛脑提取液无论局部应用或全身应用均能促进大鼠脊髓损伤后神经纤维的修复与再生，但以硬膜内给药为佳。     

COL8A1siRNA对肝癌细胞体外增殖、侵袭能力及右旋柠烯药敏性的影响

目的 应用RNA干扰技术下调小鼠肝癌细胞株(Hca-F)COL8A1的表达,通过观察癌细胞的体外增殖、侵袭能力,研究调节COL8A1表达对肿瘤细胞药敏性的影响.方法 采用RTPCR、Western Blot分析干扰前后Hca-F细胞中COL8A1的表达情况.用体外侵袭实验检测干扰前后Hca-F细胞的侵袭能力.用MTT法分析干扰前后Hca-F细胞的增殖情况及其对右旋柠烯(D-limonene)的药敏性.结果 Hca-F/RNAi细胞中COL8A1的表达与未给予RNA干扰处理的对照组及RNA干扰对照组相比出现明显下调;该细胞的增殖及穿过基质胶的侵袭能力下降;同时上调该细胞对D-limonene的药敏性.结论 小鼠肝癌细胞中COL8A1的表达与肿瘤细胞的增殖、侵袭及对抗肿瘤药物的敏感性密切相关,可能为肿瘤的化学治疗提供新靶点.

Abstract：

Objective To investigate the possible effects of COL8A1 on the proliferation, invasion and drug sensitivity of murine hepatocarcinoma cell line Hca-F, we used an RNA interference (RNAi) approach to silence COL8A1 expression. Methods The expression levels of COL8A1 in HcaF/siRNA cells were assessed by RT-PCR and Western blot. The inhibitory effect of RNAi on Hca-F cell invasion in vitro was demonstrated by ECM invasion assay. The in vitro proliferative ability and drug sensitivity of COL8A1-deficient cells were determined by MTT assay. Results The expression of COL8A1 was significantly reduced in COL8A1/siRNA cells after 30h transfection, compared with both the RNAi control and the Hca-F cells. The reduced COL8A1 expression also attenuated the proliferative, invasive ability, as well as increased drug sensitivity of Hca-F/siRNA cells. Conclusion Our current results indicate that the expression of COL8A1 functionally mediates tumor cell proliferation, invasion, and drug sensitivity, and is a potential target for therapeutic anti-cancer drugs.     

阴茎纤维性海绵体炎的研究进展

阴茎纤维性海绵体炎发病率并不低 ,长期不愈会导致阴茎弯曲、勃起疼痛及勃起功能障碍 ,临床应引起足够重视。本文就本病病因、诊断方法和治疗方法的研究进展加以综述     

经皮肾穿刺造瘘术与开放手术治疗肾后性急性肾功能衰竭的疗效对比分析

目的 探讨经皮肾穿刺造瘘术(PCN)治疗肾后性急性肾功能衰竭(PARF)患者的效果及应用价值.方法 回顾性总结分析40例PARF患者在B超引导下行PCN的临床资料,并与20例行常规开放手术患者进行对比.结果 肾穿刺组PCN成功率为100%,引流后2～7 d,患者肾功能均恢复正常;治愈30例;长期留置肾造瘘管10例,其中2例为腹腔继发肿瘤患者,8例为宫颈癌晚期患者;无一例死亡、无造瘘后肾周血肿和胸膜损伤发生,未出现术后感染、多器官功能衰竭等并发症.开放手术组于引流后2～7 d 19例患者肾功能恢复正常,其中术后出现肺部感染3例,经抗感染、对症治疗后好转;多器官功能衰竭死亡1例.结论 PCN具有创伤小、出血少的优点,对挽救PARF患者肾脏功能有重要价值.     

城镇职工基本诊疗项目的筛选及其支付标准的确定

主要探讨了筛选基本诊疗项目及其确定支付标准的一种方法，综合运用了层次分析法、德尔菲法和线性规划等方法，得到了比较理想的支付标准。这对于促进我国城镇职工基本医疗保险的完善和发展具有积极的推动意义。     

三氧化二砷对前列腺癌DU-145细胞周期阻滞及凋亡的影响

目的探讨三氧化二砷(As2O3)对前列腺癌非雄激素依赖细胞系DU-145细胞的增殖抑制作用及对细胞周期和凋亡的影响.方法应用体外细胞生长抑制试验(MTT比色法)研究As2O3对DU-145细胞生长的影响,流式细胞仪检测细胞周期分布情况及凋亡情况,琼脂糖凝胶电泳检测细胞DNA的变化.结果As2O3对DU-145细胞的增殖有明显的抑制作用,随着药物浓度增加和作用时间延长,凋亡细胞明显增加,出现G2/M期阻滞和DNA的断裂.结论As2O3可明显抑制前列腺癌DU-145细胞的增殖,诱导细胞凋亡,抑制细胞周期.     

β-榄香烯对人膀胱癌BIU-87细胞磷脂代谢转换率及细胞脂膜流动性的影响

磷脂是细胞膜的重要组分，肌醇磷脂在细胞的功能调控中发挥重要的作用，并与细胞增殖及肿瘤形成关系密切。β-榄香烯是中药温莪术的有效抗癌成分，临床应用对许多恶性肿瘤的疗效确切，我们已经证明其可诱导人膀胱癌BIU-87细胞凋亡；本研究旨在进一步研究其对BIU-87细胞肌醇磷脂代谢转换及脂膜流动性的影响，探索凋亡的分子机制，结果报道如下。     

必须认真提高男性绝育的手术质量——男扎术后痛性结节的病因和治疗

男性绝育手术,不论是输精管结扎手术还是注射法绝育术,都是我国目前大力开展计划生育工作、控制人口的迅速增长的重要的有效措施之一。由于男性的解剖和生理特点,其手术方法较女性经腹腔的输卵管结扎更为简便、安全、易行。虽然目前全国各地区都有各自习用的方法,但归纳起来不外有四川、上海、黑龙江、江苏、辽宁等地常用的“钳穿法”,河北的“穿刺引线法”,山东的“针头固定小切口法”和浙江的“针挑法”等。此外还有近年来四川省创用的“直视钳穿法”和“输精管注射绝育法”。以上虽各具特点,但总的都要求做到创伤小,安全可靠、并发症少、容易操作、广大     

蟾酥对小鼠膀胱癌的抗癌作用研究

选用小鼠可移植性膀胱癌BTT739作为动物模型，观察蟾酥对小鼠肿瘤生长以及相关指标的影响。目的：探讨蟾酥对BTT739荷瘤膀胱癌小鼠的抗肿瘤作用。方法：BTT739肿瘤细胞接种T739小鼠，随机分组，①对照组：每日腹腔注射生理盐水0．1ml1次；②蟾酥组：蟾酥按5mg／k小鼠体重腹腔注射给药，日1次；③丝裂霉素C组(MMC组)：每日1次丝裂霉素C腹腔注射1mg／kg；测定蟾酥对荷瘤小鼠的抑瘤率，荷瘤生存时间，腹腔巨噬细胞活性。结果：蟾酥组小鼠的抑瘤率为50．5％，荷瘤生存时间为31．3天，小鼠腹腔巨噬细胞吞噬率为36％。结论：在表实验条件下，蟾酥可以直接抑制小鼠膀胱肿瘤生长，延长小鼠荷瘤生存时间，提高小鼠免疫力可能是蟾酥抗小鼠肿瘤生长的重要机制。     

十全大补汤对荷膀胱癌小鼠大剂量化疗的减毒增效作用

Objective To investigate the toxicity attenuation and efficacy potentiation effects of Shiquan Dabu Tang (SDT) on high dose chemotherapy in T739 mice with bladder carcinoma. Methods Mouse bladder carcinoma tissue was inoculated subcutaneously into T739 mice to establish tumor-beating mice model. The tumor-bearing mice were randomly divided into a CTX group (100, 200, 400 mg/Kg respectively), a SDT group (high or low dose respectively), a high-dose SDT combined with 200 mg/Kg CTX group and a control group. The body weight, diameter of tumor nodules and complete blood count were observed subsequently. Results Different doses of SDT could effectively inhibit tumor growth in mice. SDT + CTX treatment significantly prolonged the survival time of mice by 49.4±23.3 days (P<0.01, 0.05, 0.01), compared with high dose SDT treatment (17.4±5.77) days, 200 mg/kg CTX treatment (23±14.02) days and control group (11.75±2.06) days respectively. The peripheral platelet count increased more significantly in mice treated with SDT within a week as compared to mice without SDT treatment (P<0.05). The peripheral RBC count and liB concentration increased more significantly in mice treated with SDT for 2 weeks as compared to mice without SDT treatment (P<0.05). Conclusions SDT could enhance the anti-tumor effects of high dose CTX on tumor-bearing mice and reduce toxicity in its peripheral red blood cells. The results showed that SDT combined with high dose of CTX chemotherapy had toxicity attenuation and efficacy potentiation effects in tumor-beating T739 mice.