罗氏海盘车中一个新的脑苷脂类化合物——罗氏脑苷D

目的:研究海洋生物罗氏海盘车的化学成分。方法:采用硅胶柱色谱、凝胶柱色谱、制备HPLC等手段分离化合物,利用理化性质和波谱分析方法鉴定结构。结果:从罗氏海盘车中分离得到1个化合物,2-{2′-羟基(2′R,14Z)二十三酰胺}-3-羟基-(2S,3R,4E)-十三碳烯-1-O-β-D-吡喃葡萄糖苷。结论:该化合物为新化合物,命名为罗氏脑苷D。     

HPLC-ESI-MS鉴定罗氏海盘车中的多种化合物及相关指纹图谱研究

目的：建立高效液相色谱-电喷雾质谱（HPLC-ESI-MS）联用技术用于海洋中药罗氏海盘车有效成分快速鉴定及其指纹图谱研究的方法。方法：对罗氏海盘车药材进行超声波提取，采用HPLC-ESI-MS 对罗氏海盘车粗提物中多种化合物进行分析，并在此基础上建立罗氏海盘车药材的HPLC 特征指纹图谱。结果：结合文献，应用HPLC-ESI-MS 鉴定出罗氏海盘车提取物中的11 种化合物；在化合物鉴定的基础上，建立了罗氏海盘车药材的HPLC 指纹图谱，发现不同批次罗氏海盘车的质量存在差异。结论：本方法可用于罗氏海盘车药材有效成分的快速鉴定及质量控制。     

海星脑苷脂-磷脂复合脂质体抗肿瘤活性作用

目的通过体内、体外实验,研究海星脑苷脂-磷脂复合脂质体的抗肿瘤作用。方法从罗氏海盘车中提取脑苷脂和磷脂,并制备脑苷脂-磷脂复合单室脂质体,采用四甲基偶氮噻唑蓝(MTT)法测定海星脑苷脂-磷脂复合脂质体对S180细胞生长抑制作用;通过体内实验检测其对S180小鼠实体瘤生长的抑制率、脾脏指数、胸腺指数及体重变化等指标。结果海星脑苷脂-磷脂复合脂质体在体外对S180细胞有明显的抑制作用,其24、487、2 h的半数抑制浓度(IC50)分别为199.89、148.95和126.65μg/mL;体内实验表明,海星脑苷脂-磷脂复合脂质体能明显抑制小鼠S180肉瘤的生长,其抑瘤率为72.6%,同时提高荷瘤小鼠的胸腺指数和脾指数,延缓荷瘤小鼠体重的下降,抑癌效果优于海星脑苷脂和磷脂单独使用。结论海星脑苷脂-磷脂复合脂质体在体内、体外均具有较强的抗肿瘤活性。     

DNA strand breaks, cytochrome P-450-dependent monooxygenase system activity and levels of chlorinated biphenyl congeners in the pyloric caeca of the seastar (Asterias rubens) from the North Sea

Seastars (Asterias rubens L.) were collecte d at sampling locations in different areas along transects radiating into the southern North Sea, representing areas impacted by contaminants to different degrees. Strand breakage in DNA isolated from tissue of the pyloric caeca was measured by the alkaline unwinding assay, modified to allow for the isolation of highly intact DNA. The interpretation of the results is based on the incidence of DNA strand breaks (expressed as the double:total DNA ratio or F value, indicating the degree of double-strandedness). The cytochrome P-450 concentration and the benzo [a] pyrene (BaP) hydroxylase activity were measured in microsomal fractions of the pyloric caeca. The chlorinated biphenyl (CB) congeners in tissue samples from the digestive gland were determined by temperature-programmed gas-chromatography, with a CPSil8 capillary column as the stationary phase, hydrogen as the carrier gas and 63Ni-electron capture detection. Areas where seastars showed different DNA integrity could be described. The highest integrity (0.75 < F < 0.85) was found in the off-shore reference sites of the Dogger Bank and Southern Bight. The mean F value in seastars from most sampling locations varied between 55 and 75%. The lowest DNA integrity (0.35 < F < 0.55) was found in specimens obtained from sampling locations near the river Rhine delta, along the Dutch coast and at two expected uncontaminated offshore areas. The BaP hydroxylase activity was relatively high near the mouth of the rivers Rhine and Scheldt, but also at a supposedly clean site near Dogger Bank. The concentration of CB congeners in the pyloric caeca of seastars decreased along transects radiating into the southern North Sea from the coastal areas of the Netherlands; the highest concentrations were in the nearby coastal areas and the lowest were in the open sea sampling locations. The data indicate that there might be a relationship between pollution from the Rivers Rhine, Meuse and Scheldt and the incidence of DNA strand breaks and/or BaP hydroxylase activity     

海星类动物的应用研究：Ⅰ罗氏海盘车壳部分化学成分的研究

对我国渤海湾产的罗氏海盘车的无机元素、氨基酸及蛋白质等成分进行了分析检测。结果显示,其壳中不仅含有大量钙、磷、镁、铯、锶,还含有一定量人体必要的锌、锡、锰、铁、锗、铜等微量元素;氨基酸总含量为17.04％,其中必要氨基酸约占30％;蛋白质含量为19.66％。     

基于棘的显微结构的海盘车属形态学研究

目 的 研究海盘车属的棘的形态学差别。方 法 借助显微照相和扫描电镜等新的方法,对中国海域海盘车属4种海盘车的棘进行研究。结 果 记录4种海盘车棘的数量排列和形态,同时对不同部位棘进行拍照记录,归纳出基于棘显微结构的检索表。     

多棘海盘车的化学成分研究进展

国内外对多棘海盘车的化学成分进行大量的研究,从中分离得到多种独特的化学成分及新成分,包括海星皂苷、甾体硫酸盐、甾体、氨基酸、多糖等,其中很多化合物具有促进抑菌和溶血等方面的药理活性,查阅和收集国内外相关文献进行归纳、分析和综述,为多棘海盘车的进一步开发研究提供依据。     

海星Asterias rollestoni的化学成分研究

目的从青岛海星Asterias rollestoni分离并确定结构新颖的活性化合物,并阐明海星中的次生代谢产物的多样性.方法应用正相和反相色谱包括半制备HPLC方法分离和纯化化合物;应用IR,UV,MS,lD和2D NMR,以及理化性质确定化合物的结构.结果从青岛海星Asterias rollestoni中获得17种化学成分,分别为N7-2'-deoxypseudoxanthosine(1),2'-O-methyl-in-osine(2),2'-deoxyinosine(3),2'-O-methylguanosine(4),inosine(5);thymine(6),uracil(7),thymidine(8),deoxyuridine(9),2'-O-methyluridine(10),(-)-(1S,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(11),(-)-(1R,3S)-1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(12),(-)-(3S)-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid(13),L-tryptophan(14),L-phenylalanine(15),3-carboxy indole(16)和p-hydroxybenzoic acid(17).结论其中1为新化合物.它们的化学结构经1D,2D NMR,以及其它波谱分析,结合与已知化合物的波谱和理化数据对照得以确定.化合物8,9,10为首次从天然资源中获得,化合物14和15系首次从该海星中获得.     

海星黄对肾虚大鼠及肾阳虚志愿者的影响

研究海星黄对房劳型大鼠及肾阳虚证志愿者的影响，结果表明，口服给予海星黄2g.d^-1,90d后大鼠组的红细胞数，白细胞数，血红蛋白含量，肾指数，睾丸指数和精囊腺指数均比造型组高，海星黄对肾阳虚志愿者总有效率为90％，所有服用本品者未发现任何不良反应，说明海星黄对大鼠肾阳虚具有显著的滋补作用，对肾阳虚证病人有较好的治疗作用。     

罗氏海盘车、海燕、金氏真蛇尾皂甙的制备及性质鉴定比较

采用甲醇法分分别对罗氏海盘车、海燕、金氏真蛇尾３种棘皮动物的皂甙进行了提取制备,其产率分别为０．０２３３％、０．０５２２％、０．１１５４％,结果表明金氏真蛇尾的皂甙含量最高,并且对３种皂甙进行理化性质实验、溶血实验、紫外光谱和红外光谱和红外光谱鉴定比较,证明提取物为海星总皂甙,由罗氏海盘车、海燕、金氏真蛇尾提取的皂甙溶点分别为１７５～１７８℃、２４２～２５５℃、２４４～２６０℃。