| HPLC-ESI-MS鉴定罗氏海盘车中的多种化合物及相关指纹图谱研究 |
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| 引用本文： | 张道来,陈军辉,王 虹,赵恒强,程红艳,王小如,李国强.HPLC-ESI-MS鉴定罗氏海盘车中的多种化合物及相关指纹图谱研究[J].世界科学技术-中医药现代化,2009,11(1):173-178. |
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| 作者姓名： | 张道来 陈军辉 王 虹 赵恒强 程红艳 王小如 李国强 |
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| 作者单位： | 国家海洋局第一海洋研究所青岛市现代分析技术及中药标准化重点实验室,青岛,266061;国家海洋局第一海洋研究所青岛市现代分析技术及中药标准化重点实验室,青岛,266061;国家海洋局第一海洋研究所青岛市现代分析技术及中药标准化重点实验室,青岛,266061;国家海洋局第一海洋研究所青岛市现代分析技术及中药标准化重点实验室,青岛,266061;国家海洋局第一海洋研究所青岛市现代分析技术及中药标准化重点实验室,青岛,266061;国家海洋局第一海洋研究所青岛市现代分析技术及中药标准化重点实验室,青岛,266061;厦门大学化学化工学院化学系现代分析科学教育部重点实验室,厦门,361005;中国海洋大学医药学院,青岛,266003 |
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| 基金项目： | 我国近海海洋综合调查与评价“908”专项（908-02-05-04）：海洋药用生物资源评价和《中华海洋本草》编纂，负责人：王长云；海洋一所基本科研业务专项（GY-022008T32）：海洋绿藻活性成分分析、鉴别，负责人：陈军辉。 |
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| 摘 要： | 目的：建立高效液相色谱-电喷雾质谱（HPLC-ESI-MS）联用技术用于海洋中药罗氏海盘车有效成分快速鉴定及其指纹图谱研究的方法。方法：对罗氏海盘车药材进行超声波提取，采用HPLC-ESI-MS 对罗氏海盘车粗提物中多种化合物进行分析，并在此基础上建立罗氏海盘车药材的HPLC 特征指纹图谱。结果：结合文献，应用HPLC-ESI-MS 鉴定出罗氏海盘车提取物中的11 种化合物；在化合物鉴定的基础上，建立了罗氏海盘车药材的HPLC 指纹图谱，发现不同批次罗氏海盘车的质量存在差异。结论：本方法可用于罗氏海盘车药材有效成分的快速鉴定及质量控制。
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| 关 键 词： | 高效液相色谱-电喷雾质 谱罗氏海盘车 指纹图谱 |
| 收稿时间： | 2008/11/4 0:00:00 |
| 修稿时间： | 1/6/2009 12:00:00 AM |
Identification of active components in Asterias rollestoni Bell. by HPLC- ESI-MS and Study on Their HPLC Fingerprint |
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| Zhang Daolai,Chen Junhui,Wang Hong,Zhao Hengqiang,Cheng Hongyan,Wang Xiaoru and Li Guoqiang.Identification of active components in Asterias rollestoni Bell. by HPLC- ESI-MS and Study on Their HPLC Fingerprint[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2009,11(1):173-178. |
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| Authors: | Zhang Daolai Chen Junhui Wang Hong Zhao Hengqiang Cheng Hongyan Wang Xiaoru and Li Guoqiang |
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| Institution: | QingDao Key Lab on Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of SOA, Qingdao 266061;QingDao Key Lab on Analytical Technology Development and Standardization of Chinese Medicines, First Institute Oceanography of SOA, Qingdao 266061; Department of Chemistry and the Key Laboratory of Analytical Science of the MOE, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China;School of Medicine and Pharmacy, Ocean University of China, Qingdao 266063, China |
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| Abstract: | Objective: A new method based on high performance liquid chromatography- electrospray ionization mass spectrometry (HPLC-ESI-MS) was developed for the rapid identification of active compounds in Asterias rollestoni Bell and development of its fingerprints. Method: Samples were extracted by ultrasonic-assist extraction, and the extraction conditions were optimized. The developed HPLC -ESI -MS method was used to identification of the components in Asterias rollestoni Bell extract, and a chromatographic fingerprint based on HPLC analysis was established. Result: Eleven compounds in Asterias rollestoni Bell extract could be primary identified by ESI-MS on-line detection combined with literature review. The result of similarity evaluation for fingerprint indicated that the quality of different Asterias rollestoni Bell samples were not entirely consistent. Conclusion: This method has the advantages of simple operation, rapid measurement and it is a powerful tool for identification of active components in Asterias rollestoni Bell and its quality control. |
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| Keywords: | HPLC-ESI-MS Asterias rollestoni Bell Fingerprint |
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