OBJECTIVE: To understand the role of nasal mucous epithelial cells to hypoxia in early stage of nasal polyps(NP) formation. METHODS: Epithelial cells of NP and inferior turbinate (IT) were cultured without serum under normal oxygen and hypoxia, and stimulus of inflammatory cytokines. Erythropoietin (EPO) was regarded as hypoxia mark, and expression of vascular endothelial growth factor(VEGF) mRNA and protein derived from epithelial cells were detected respectively by in situ hybridization and ELISA. RESULTS: 1. Under hypoxia, EPO mRNA was expressed intensely in epithelial cells from NP and IT, and there was no significant difference between both of them. This result suggested that EPO might be regarded as a hypoxic mark. 2. The ability of producing VEGF mRNA increased with cytokines stimulation, especially under hypoxia. Protein level of VEGF from epithelial cells of NP and IT increased with cytokines stimulation, especially in hypoxia and was time-dependent. CONCLUSION: Epithelial cells actively produce vast VEGF under hypoxia. The VEGF induced by hypoxia of the mucosa in middle meatus is of importance in the formation of nasal polyps(NP) in early stage, which may be the major cause of NP formation in middle meatus. 相似文献
To analyze the clinical and pathological features of patients with anti-neutrophilic cytoplasmic antibody (ANCA)-associated vasculitic (AASV)-kidney injury (AASVKI).
Methods
From January 2015 to December 2018, a total of 101 AASVKI patients treated in the First Hospital of Jilin University were divided into 2 groups (the pANCA-positive group and the cANCA-positive group) for comparison; 63 patients were performed renal biopsy and divided into 3 groups according to pathological results [the non-crescent nephritis group (non-C), the crescent nephritis group (C), and the sclerotic nephritis group (S)] for comparison.
Results
Compared with the Group pANCA, Group cANCA exhibited higher incidence of eye involvement (P?=?0.039) and skin mucosa destruction (P?=?0.045), higher serum creatinine (Scr) (P?<?0.001), higher 24-h urine protein quantification (P?=?0.045), but lower hemoglobin (Hb) (P?<?0.001). Compared with Group non-C, Group C had higher Scr (P?<?0.001) and urinary red blood cells (P?=?0.010), lower estimated glomerular filtration rate (eGFR) (P?=?0.003), hemoglobin (HD)-dependence (P?=?0.042), and higher mortality rate within 1 year (P?=?0.005); compared with Group S, Group C had faster onset of erythrocyte sedimentation rate (ESR) (P?=?0.031), higher urinary red blood cells (P?=?0.029), and lower incidence of HD-dependence (P?=?0.038).
Conclusions
AASVKI patients with positive cANCA have poor renal function, among whom the patients with crescent type have poorer renal function than those with non-crescent type, have higher urinary red blood cells while lower HD-dependence than those with sclerotic type.