HPLC法测定益母草颗粒中盐酸水苏碱含量

益母草颗粒本品种为卫生部药品标准中药成方制剂第7册收载的品种，原标准中仅有益母草中生物碱的沉淀反应。为提高本品的质控标准，以更好的控制药物的内在质量，本文采用HPLC法对方中益母草进行了测定。含测试验中，采用HPLC法，以盐酸水苏碱为对照品，并参考有关文献，对方中盐酸水苏碱进行了HPLC含量测定方法的研究，通过方法学的系统考察和10批样品的含量测定，建立了本品中盐酸水苏碱的高效液相色谱含测方法，现报道如下。     

光纤传感溶出度监测系统测定萘普生钠胶囊溶出度

目的:建立萘普生钠胶囊体外溶出度的测定方法.方法:采用桨法,转速100 r·min-1,以pH 6.8磷酸盐缓冲液为溶出递质,利用萘普生钠在330 nm处的紫外吸收,用光纤化学传感溶出度过程分析系统(FOCSDT)原位监测萘普生钠胶囊溶出过程.同时取样,用紫外分光光度法测定溶出度,作对照实验.结果:在24.6～123.2 mg·L-1范围内,测定信号与萘普生钠的浓度成线性关系.两种测定方法差异无显著性(P＞0.05).结论:FOCSDT药物溶出度过程分析系统连续完整地反映了药物溶出情况,提供了快速溶出药物溶出度监测的可信方法.     

真菌性鼻窦炎20例临床分析

目的 探讨真菌性鼻窦炎的诊断和治疗,提高对本病的认识,减少误诊。方法 对20例真菌性鼻窦炎的临床资料进行回顾性分析。结果 真菌性鼻窦炎CT扫描主要特点为鼻窦炎内软组织密度影,仅极少数伴有不均匀的斑片状或点状钙化灶。20例均经手术及术后配合抗真菌药物治疗治愈,并经病理检查找到真菌菌丝。结论 本病的诊断主要依靠病理,过份强调CT的特征性改变容易造成误诊。手术彻底清除病变组织,开放窦口,保证引流,术后配合抗真菌药物治疗是有效的治疗方法。     

喉颗粒细胞瘤1例

病人，女，10岁，因声嘶进行性加重6个月，以左声带肿物性质待查于2002年10月10日人院。查体：一般情况好，会厌呈小儿型，抬举尚可，间接喉镜和纤维喉镜检查均示：左声带后1／3有一白色圆形肿物，表面光滑，双声带运动正常。于2002年10月14日在气管插管全麻支撑喉镜下行左声带肿物摘除术，术中见左声带中后部见一新生物，约2mm     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.     

阻塞性睡眠呼吸暂停低通气综合征与左心室肥厚相关性研究

[目的]探讨阻塞性睡眠呼吸暂停低通气综合征(Obstructive Sleep Apnea Hypopnea Syndrome,OSAHS)与左心室肥厚(Left Ventricular Hypertrophy,LVH)的相关性。[方法]随机抽取我院心血管内科住院病人63例,进行夜间多导睡眠监测(Polysomnography,PSG)及心脏彩超检查,并根据PSG结果分成正常组、轻度组及中重度组。[结果]轻度及中重度组的室间隔厚度(interventricular septum thickness in diastole,IVSd)及左心室后壁厚度(left ventrieular posterior wall thickness in diastole,LVPWd)高于正常组且与睡眠呼吸暂停低通气指数(apnea hypoventilation index,AHI)存在正相关。AHI与IVSd、LVPWd的回归系数分别为0.061(P<0.05)及0.071(P<0.05),即在其它条件不变的情况下AHI每增加10,IVSd可增加0.61 mm,LVPWd可增加0.71 mm。[结论]OSAHS与LVH存在正相关。OSAHS可能是引起LVH的原因之一。     

面中掀翻上颌骨拆装进路切除鼻咽血管纤维瘤

目的:探讨应用面中掀翻上颌骨拆装进路治疗Ⅲ、Ⅳ期鼻咽血管纤维瘤患者的可行性。方法:回顾性分析2001年2月~2004年8月应用面中掀翻上颌骨拆装进路治疗7例Ⅲ、Ⅳ期鼻咽血管纤维瘤的临床资料。采用Fisch分期,Ⅲ期患者5例,Ⅳ期患者2例;2例Ⅲ期患者接受面中掀翻联合同侧上颌骨拆装进路,接受面中掀翻联合LefortⅠ患者5例(Ⅲ期3例、Ⅳ期2例),其中1例Ⅳ期患者因颅内肿瘤侵犯范围较广采用颅面联合进路。6例术前行超选择性瘤体供血动脉栓塞(Ⅲ期患者4例、Ⅳ期患者2例)。结果:术中出血量为(600±324)ml。6例动脉栓塞患者术中出血量为(483±165)ml。1例未栓塞患者出血量1 300 ml。手术时间为(129±22)min。7例患者术后病理为鼻咽血管纤维瘤。6例患者术后随访2.5~6.0年未见肿瘤复发。1例接受术前行超选择性瘤体供血动脉栓塞Ⅲ期患者术后1年肿瘤复发,再手术后随访至今未见复发。结论:对于Ⅲ、Ⅳ期患者,面中掀翻上颌骨拆装不仅利于术中显露肿物达到根治性切除,缩短手术时间、减少术中出血量而且术后美容佳、功能恢复好。术前行血管造影及血管栓塞对减少术中出血量、降低肿瘤复发率具有重要临床意义。     

钙离子拮抗剂治疗动脉粥样硬化的现状

<正>钙离子拮抗剂(CCB)是目前心血管领域内最常应用的药物之一,它主要用于治疗高血压病、心绞痛、心律失常、肥厚型心肌病及慢性心功能不全等方面,是目前应用非常广泛和     

军队疗养院抗菌素使用调查分析

目前合理应用抗菌素已成为军队疗养院面临的严重问题,不合理应用抗菌素的危害已被人们逐渐重视,本文对我院2002-2003年抗菌素应用处方统计分析,加深了解军队疗养院门诊抗菌药物应用现状,对不合理用药加重细菌耐药及不良反应严重性的认识,对增强合理应用抗生素的责任心与紧迫感有重要的意义。1　疗养院不合理使用抗菌素的现象11　无指征或指征不明显用药　预防用药实际只限于少数必要的情况下,不能盲目无原则地滥用。如果无论是否发生感染,普遍给予抗生素只能造成不必要的浪费和增加患者的经济负担,也可能使细菌产生耐药性,菌群失调,增加…     

两种膨润土细胞毒性的体外试验研究

Objective To study comparatively the cytotoxicity induced by acid bentonite and organic bentonite.Methods The cytotoxicity of two kinds of bentonite was detected using CCK8 assay,neutral red uptake(NRU) assay,lactate dehydrogenase(LDH) leakage assay,apoptosis assay and hemolysis assay.In hemolysis assay human erythrocytes served as target cells and were exposed to the two kinds of bentonite at the doses of 0,0.3125,0.6250,1.2500 and 2.5000 mg/ml for ten min.In other four assays,human B lymphoblast cells (HMy2.CIR) served as target cells and were exposed to the two kinds of bentonite at the doses of 0,10,20,30,60,120 and 180 μg/ml for four h.Results In hemolysis assay,the hemolysis rates induced by two kinds of bentonite at all doses were significantly higher than that of control (P＜0.05);in CCK-8 assay,the cellular activities in acid bentonite group at the doses ≥30 μg/ml and in organic bentonite group at the doses ≥20μg/ml were significantly lower than that of control (P＜0.01);the similar results appeared in NRU assay and LDH assay,and the dose-effect relationship was observed in above 4 assays.In apeptosis assay,the early apoptosis cell rates in acid bentonite group at the dose of 180 μg/ml and in organic bentonite group at the doses of 120,180 μg/ml were significantly higher than that of control (P＜0.05).Moreover,the results of five in vitro assays indicated the eytotoxicity induced by organic bentonite was higher than that induced by acid bentonite.Conclusion Two kinds of bentonite could induce cytotoxicity,such as apoptosis and damage of cell membrane.The cytotoxicity of organic bentonite is higher than that of acid bentonite due to the different industrial treatment and characteristics of two kinds of bentonite particles.