高压氧对慢性应激抑郁模型大鼠行为及神经干细胞移植的影响

BACKGROUND:Hyperbaric oxygen as a well-recognized therapy for ischemic and hypoxic diseases has to be combined with other treatments. OBJECTIVE:To study the effects of hyperbaric oxygen combined with neural stem cell transplantation via tail vein in chronic stress depression rats. METHODS:Of 60 Sprague-Dawley rats, 15 rats randomly selected were given no treatment as normal group, and the rest 45 ones were used to establish a rat model of depression and randomly divided into three groups: model group (n=15, without giving any treatment), neural stem cell group (n=15, injection of 1 mL neural stem cell suspension (3×10⁶) via the tail vein) and combined group (n=15, hyperbaric oxygen treatment plus neural stem cell injection). Hyperbaric oxygen treatments were carried out four times per day, for 1 week, and all the treatments were given 24 hours after modeling. Rats in each group were subjected to body mass measurement and sugar water consumption test 1 and 2 weeks after treatment, and open field test within 5 minutes after treatment to observe rat behavior changes. The survival and distribution of CM-Dil labeled neural stem cells were observed by fluorescence microscopy. The changes in the size, morphology and number of hippocampus neurons in rat were detected by the method of nylon staining. TUNEL method was used to measure the apoptosis of nerve cells. RESULTS AND CONCLUSION:Compared with the normal group, in the model group, rat’s body weight, sucrose water preference and open field test scores were significantly lower, reduced number of hippocampal neurons with intact structure was found and TUNEL results showed more apoptosis (P < 0.05). Compared with the model group, the body weight, sucrose preference and open field test score increased significantly (P < 0.05), the number of hippocampal neurons was increased, while the number of apoptotic cells was reduced significantly in the neural stem cell and combined groups (P < 0.05). Compared with the neural stem cell group, these indexes were improved more significantly in the combined group (P < 0.05). More CM-Dil positive cells were found in the combined group than the neural stem cell group (P < 0.05). Additionally, the number of hippocampal neurons was higher in the combined group than the neural stem cell group, but lower than the normal group. To conclude, transplantation of neural stem cells combined with hyperbaric oxygen can improve the depression behavior and apoptosis in the hippocampal neurons in chronic stress depression rats.     

Cofilin 1在肿瘤中的表达及意义

Cofilin 1（丝切蛋白1）是肌动蛋白相关蛋白,对肌动蛋白介导的细胞运动和骨架的改变起重要的调节作用。近年研究发现Cofilin 1的活化与肿瘤细胞的恶性侵袭性质有关。Cofilin 1的局部激活可以诱导片状伪足的形成,并影响肿瘤细胞运动的方向,从而增强肿瘤细胞的运动和迁移。最新研究成果显示,抑制或者增强Cofilin 1的活性可以减少肿瘤细胞的侵袭和迁移。Cofilin 1和p-Cofilin1在肿瘤细胞中的表达存在一个动态平衡,打破这个平衡可以直接抑制细胞的迁移和侵袭。本文对Cofilin 1的结构、功能、调控机制及平衡机制与肿瘤侵袭转移和上皮间质转化(EMT)以及肿瘤耐药性的关系作一综述。     

以艾滋病病毒gp41为靶点的芳基苯甲酸类化合物的设计、合成及活性评价

目的以艾滋病病毒（HIV）gp41为作用靶点,设计合成了12个含芳基取代的苯甲酸类化合物,并对其进行抗HIV活性测
试。方法以卤代苯甲酸或间羧基苯硼酸为原料,通过Suzuki-Miyaura偶连反应及Knoevenagel缩合反应,引入含取代基的芳环
及芳杂环。ELISA法检测其抑制HIV gp41 六螺旋束形成的活性。荧光素酶法检测化合物抗HIV活性。结果化合物结构经
NMR、MS得到确认,其中5个化合物（7b、7c、7d、7e、7g）具有抑制HIV gp41六螺旋束形成的活性,其中7d活性最强。该化合物
能抑制HIV-1 SF33病毒株的复制,其IC50为20 μmol/L。结论合成的芳基苯甲酸类化合物7d能通过抑制HIV gp41六螺旋束结
构的形成来抑制HIV的复制。
     

胸部外伤50例患者X线征象及动态观察

100039;2武警总院放射科,北京100039)胸部在直接或间接的暴力下均可发生肺挫伤、撕裂伤骨折等,严重者会危及患者生命。因此,及时准确地作出X线诊断,对减轻患者的痛苦、挽救患者生命有着非常重要意义。对胸部外伤的动态观察有助于了解病变的吸收治愈过程,为临床医师提供可靠的诊     

结核杆菌DNA疫苗pVS84与pIL2质粒联合免疫的保护效率研究

目的用构建的人hIL-2质粒和结核杆菌DNA疫苗pVS84联合免疫后,对小鼠的细胞因子进行了测定并对抗结核分枝杆菌H37Rv攻击的能力进行了评估。方法将hil-2插入pVAX1,构建了人hIL-2的质粒pIL2。将雌性C57BL/6鼠分成6组,分别用pVS84和pIL2各50μg,pVS84,pVAX1,pIL2S和PBS免疫3次,间隔2周,加强免疫2次。另一组用BCG(105CFU)皮内免疫1次。每组10只鼠在最后一次加强后,取脾培养,检测上清细胞因子。另10只用结核菌H37Rv攻击,2周后取脾、肝和肺培养结核菌并计数。结果pVS84+pIL2联合免疫组的鼠血清hIL-2平均浓度为720.5±114.5pg/ml,显著高于其它组。pVS84+pIL2组、pVS84组的脾细胞培养上清的mIL-2和mIFN-γ平均含量显著高于3个阴性对照组(P<0.001),与BCG免疫组无显著性差异(P>0.05)。pVS84+pIL2组脾、肝和肺的平均结核菌载量分别为12471.4±2269.3,13622.6±2404.0和14742.0±2378.7CFU/g,低于pVS84组和3个阴性对照组相应器官的结核菌载量(P<0.001),与BCG对照组无显著差异。结论pIL2质粒与pVS84联合免疫能够刺激机体产生Th1型免疫应答,抵抗H37Rv攻击的能力与BCG的效果相当。     

先天性二叶式主动脉瓣畸形的超声诊断价值

二叶式主动脉瓣畸形(Bicuspid aortic valve defect BAVD)是一种常见的先天性疾病,易伴发其他心血管异常,出现明显血流动力学改变及严重症状而需药物治疗或手术干预。多普勒超声心动图是全面评价BAVD及其并发症的首选方法。本文总结BAVD的超声表现,为超声及临床医师提高对该病的认识提供有用的信息。     

基质金属蛋白酶抑制剂抗肝癌细胞系侵袭作用的实验研究

目的探讨基质金属蛋白酶抑制剂-3（TIMP-3）对人肝癌细胞系（HXPG2）恶性表型的影响。方法利用基因重组技术构建含全长人TIMP-3cDNA的真核表达载体,利用脂质体法转染入HEPG2细胞。检测转染后鳓细胞的金属蛋白酶（MMPs）的活性、体外侵袭能力、制成裸鼠肝癌动物模型后体内成瘤性及自发转移能力等多项生物学行为的变化。结果HEPG2细胞在转染了TIMP-3cDNA后表达TIMP-3蛋白,与朱转染细胞及空载体对照组相比,体外侵袭能力、软琼脂集落形成能力均降低,裸鼠的肝癌肿瘤体积及重量也相应降低,且肿瘤组织中微血管密度降低,细胞外基质基本保持完整。结论特异性上调TIMP-3基因的表达可在一定程度上逆转HEPG2细胞的恶性表型。     

沂水县学龄儿童碘缺乏病防治监测结果分析

目的 了解沂水县碘缺乏病经十多年防治后,8～10岁学龄儿童的碘代谢及甲状腺肿情况.方法 触诊法测甲状腺肿,砷铈催化分光光度法测尿碘,氧化还原直接滴定法测盐碘.结果 甲状腺肿大率8.83%,患病率0.63%;尿碘中位数317.32ug/L;碘盐,盐库为33.4mg/kg,销售点为29.6mg/kg,居民户为23.3mg/kg.结论 沂水县碘缺乏状态基本改进,8～10岁学龄儿童的碘摄入量能维持体内的正常需要,但仍有部分儿童的甲状腺肿发生率较高,主要原因是这部分人补碘不足,用盐不当.     

尼莫地平治疗急性蛛网膜下腔出血疗效观察

近年来,我们应用尼莫地平治疗急性蛛网膜下腔出血(SAH)昏迷31例,取得良好效果。现总结如下。 临床资料:本组59例发病6小时内,经颅脑CT检查确诊的急性SAH患者,均无糖尿病、重度肝肾功能不全、心功能不全或多脏器功能衰竭。随机分为两组,治疗组31例,男18例,女13例;年龄42～57岁,平均49岁;意识状态评分(18.19±5.64);对照组28例,其性别、年龄、意识状态评分等与治疗组具有可比性。 治疗方法:两组均采用降低颅内压、抗纤溶止血及对症支持治疗;治疗组在此基础上用尼莫地平10mg加入5％葡萄糖生理盐水250～500ml中静脉滴注,每日1次,共10天。 结果:两组治疗前后意识状态评分见表1。表1显示,两