荧光定量RT-PCR检测Rac1 mRNA基因及其与胃癌转移的关系

Objective To investigate the relationship between tumor metastasis-related Rac1 mRNA expression levels and gastric carcinomas metastasis, and to investigate the significance of Rac1 as a tumor marker for the evaluation of gastric carcinomas metastasis and distinguish between benign and malignant lesions. Methods This experiment used fluorescence quantitative RT-PCR TaqMan probe technology, chose Rac1 target gene fragment, which was cloned into the pET-20b (+) vector, constructed recombinant plasmid, and established the Rac1 mRNA fluorescence quantitative RT-PCR standard curve. And then the Rac1 mRNA levels were detected in 52 cases of gastric carcinoma tissues,52 cases of para-carcinoma tissues and 12 cases of benign gastric disease tissues. Its association with the metastasis of gastric carcinomas was analyzed. Results The positive rates and levels (median, P25-P75) of Rac1 mRNA were 100. 0% ,7.41 ×105 (3. 50 × 105-4. 36 × 106) copies/μl in gastric carcinomas, 46. 2% ,0(0-1.73 × 104) copies/μl in parscarcinoma tissues, 33. 3%, 0(0-3.55 × 103) copies/μl in benign tissues. The positive rates and leves(x2 =43.16,x2Xk-w = 64. 19, P ＜0. 01)among the three groups were significantly different. When the critical value of Rac1 mRNA level was 1.73 × 104 copies/μl determined by ROC, the sensitivity and specificity were 88. 5% and 100% respectively. When the critical value of Rac1 level was 7.49 × 105 copies/μl, the sensitivity and specificity were 57.9% and 78. 6% respectively. ConclusionThe Rac1 mRNA level detected with fluorescence quantitative RT-PCR has reference value to distinguish between benign and malignant lesions and evaluate gastric carcinoma metastasis.     

NK细胞趋化因子和细胞因子的分泌与调节

NK细胞是固有免疫系统中一个非常重要的成员，能够杀死病毒、细菌、寄生虫感染的细胞以及肿瘤细胞。同时，NK细胞也可以分泌一些细胞因子和趋化因子来调节免疫应答以及炎症反应，然而，这种分泌功能与NK细胞的成熟状态相关，同时也受到一些调控因子的调节，比如P1108，深入研究这些因子的功能和调控过程，对NK细胞参与免疫应答以及炎症反应具有重要意义。     

孕早期环境因素对儿童先天性心脏病的影响

目的:探讨孕早期环境因素对儿童先天性心脏病(CHD)的影响。方法:收集2011年6月~2013年12月在深圳市妇幼保健院出生的汉族CHD患儿100例作为病例组,按照配对(1∶1)病例对照研究选取同期同医院同民族体检正常的儿童100例作为对照组,通过问卷调查及多因素Logistic回归分析方法,分析CHD可能的危险因素。结果:病例组患儿CHD的主要类型有室间隔缺损51例(51.0%)、房间隔缺损32例(32.0%)、动脉导管未关闭12例(12.0%)、其他5例(5.0%)。应用卡方检验筛选其中孕前6个月慢性病史、严重早孕反应、孕早期感冒、孕妇职业危险因素接触史、孕妇异常生育史、孕妇流产史、孕早期服药史、母亲被动吸烟、丈夫职业危险因素接触史、丈夫饮酒史、孕前补充维生素和补充微量元素等因素,两组间差异具有统计学意义(P<0.05);多因素Logistic回归分析发现,孕前补充维生素和微量元素与CHD发生呈现负相关关系;孕前6个月慢性病史、孕妇职业危险因素接触史、孕早期感冒、孕妇异常生育史、母亲被动吸烟和丈夫职业危险因素接触史与CHD发生呈现正相关关系。结论:儿童发生CHD与父母双方的某些习惯或者孕前的某些状况都有关系,应加强父母双方怀孕的整个过程的健康教育,保健服务以及减少对危险因素接触,从而减少和预防CHD的发生。     

NK细胞趋化因子和细胞因子的分泌与调节

Natural killer (NK) cells are the important members involved in innate immunity, which can kill virus-, intracellular bacteria-, and parasite-infected target ceils or tumor cells. NK cells can secrete cytokines/ chemokines as well, in order to mediate immune response and inflammation. However, its cytokine-secretion ability is related with the maturation of NK cells and is regulated by some factors, for example, P110δ. There-fore, more work should be done to elucidate the function and regulation of these factors, which have a signifi-cant influence on inflammation and tissue impairment in some diseases involving NK cells.     

T淋巴瘤侵袭转移诱导因子1表达水平与胃癌生物学行为的关系

目的探讨T淋巴瘤侵袭转移诱导因子1（Tiam1）在胃癌组织、癌旁组织、胃良性病变组织中的表达水平，及其与胃癌转移等生物学行为的关系。方法用实时荧光定量逆转录PCR（FQ-Rt-PCR）方法，对66例胃癌患者的癌组织、癌旁组织及11例胃良性病变患者的组织标本中Tiam1 mRNA进行定量检测。结果66例胃癌患者的癌组织、癌旁组织中Tiam1 mRNA表达阳性率分别为92．4％、19．7％，11例胃良性病变患者的组织标本中有1例患者有Tiam1 mRNA表达，3类组织标本的差异有统计学意义（χ^2=78．96，P〈0．01）；胃癌组织中Tiam1 mRNA表达量高于癌旁组织，且与患者性别、年龄、肿瘤大小、肿瘤原发部位无关（P〉0．05），但随着淋巴结转移的发生、浸润深度加深、分化程度降低、TNM分期增高，而呈现增高趋势（P〈0．05）。结论FQ-Rt-PCR检测Tiam1表达具有较高的敏感性和特异性，Tiam1与胃癌生物学行为密切相关，有可能成为胃癌转移的基因治疗新靶点。     

P一糖蛋白基因疫苗诱导小鼠实验性肝炎

目的：观察P-糖蛋白基因疫苗免疫小鼠诱导的免疫病理变化。方法：P-糖蛋白基因疫苗20～100μg两次肌注(间隔9d)免疫小鼠，收集血清检测特异性抗体效价；取心、肝、肾制作组织切片，HE染色行病理检查。结果：末次免疫后5d鼠血清特异性抗体效价达到1：4000；心、肾组织正常，但肝脏出现自身免疫性肝炎改变。结论：P-糖蛋白基因疫苗免疫小鼠可诱导实验性肝炎，实验性肝炎小鼠可作为人自身免疫性肝炎的动物模型。     

溴隐亭治疗高泌乳素血症42例分析

高泌乳素血症常导致不孕及卵巢功能减退，本文回顾1994年3月～1995年6月本院妇科门诊诊治的高泌乳素血症病人共42例，采用澳隐亭治疗，对其就诊原因及治疗效果，作一回顾性分析。1资料与方法1．1一般资料1994年3月～1995年6月，在我院妇科门诊收集的高泌乳素血症42例，无甲状腺功能疾患及影响PRL升高用药史，年龄21岁至40岁。1．2；仅床资料42例为已婚，结婚最长12年，最短一年，平均4．2年，其中以不孕、闭经、溢乳为主要临床表现，其中23例有生育要求。闭经时间3个月～5年，溢乳时间为2个月～3年，42例患者中，CT发现垂体微腺癌7例。1．…     

NK细胞趋化因子和细胞因子的分泌与调节

Natural killer (NK) cells are the important members involved in innate immunity, which can kill virus-, intracellular bacteria-, and parasite-infected target ceils or tumor cells. NK cells can secrete cytokines/ chemokines as well, in order to mediate immune response and inflammation. However, its cytokine-secretion ability is related with the maturation of NK cells and is regulated by some factors, for example, P110δ. There-fore, more work should be done to elucidate the function and regulation of these factors, which have a signifi-cant influence on inflammation and tissue impairment in some diseases involving NK cells.     

NK细胞趋化因子和细胞因子的分泌与调节

Natural killer (NK) cells are the important members involved in innate immunity, which can kill virus-, intracellular bacteria-, and parasite-infected target ceils or tumor cells. NK cells can secrete cytokines/ chemokines as well, in order to mediate immune response and inflammation. However, its cytokine-secretion ability is related with the maturation of NK cells and is regulated by some factors, for example, P110δ. There-fore, more work should be done to elucidate the function and regulation of these factors, which have a signifi-cant influence on inflammation and tissue impairment in some diseases involving NK cells.