都市白领滋补茶

都市丛林中，优雅的职业女性们一如铿锵的玫瑰，继续坚强地绽放着、憔悴着。亚健康的状态无处不在。让我们来帮你找个温情、传统的伴侣吧!关爱自己，从药饮开始……     

肿瘤Ki-67抗原HLA-A2限制性细胞毒性T淋巴细胞表位的鉴定

Objective To identify the HLA-A2-restricted CTL epitopes encoded by antigen segment of Ki-67 for preparation of tumor vaccines. Methods Two epitope prediction databases ( BIMAS and SYFPEITHI) were used to predict the epitopes of Ki-67, and 7 peptides were synthesized as the candi-dates. The prediction was evaluated by peptide-binding test. ELISPOT was used to inspect the IFN-γ secre-ted by CTLs in order to reflect the immunogenicity of the peptides. Results The purities of synthetic pep-tides and positive control peptide were all above 95%. Peptide-binding test displayed that LQGETQLLV (280-288) of Ki-67 could bind with HLA-A2 molecule more strongly. ELISPOT assay displayed that LQGETQLLV could induce activation of specific CTLs. Conclusion LQGETQLLV (280-288) might be the HLA-A2-restricted CTL epitope of Ki-67 antigen.     

直肠癌Miles术后患者自我形象紊乱的诊断和护理

目的　探讨自我形象紊乱 (self-imagedisorder)这一护理诊断在直肠癌Miles术后患者中的应用及其相关护理措施。方法　对 78例直肠癌Miles术后患者进行资料收集和问卷调查分析 ,同时比较不同年龄、性别、文化程度患者自我形象紊乱的发生率 ,并针对患者的特点进行护理干预。结果　 78例患者中 4 6例发生自我形象紊乱 ,占 5 8.97% ,发生率随受教育程度的升高而降低 ,差异极显著 (P <0 .0 1)。老年患者的发生率较年轻患者高 ,女性较男性稍高 ,但无统计学差异。结论　自我形象紊乱在直肠癌Miles术后患者中的发生率较高 ,尤其是老年、文化程度较低的患者发生率更高。自我形象紊乱影响患者的术后恢复 ,影响其生活质量 ,因此应予以充分重视 ,积极治疗 ,使患者恢复信心 ,提高生活质量。     

Multi-parameter prediction of HLA class Ⅰ restricted cytotoxic T lymphocyte epitopes for human proliferating cell nuclear antigen

Objective To predict the HLA class Ⅰ restricted cytotoxic T lymphocyte epitopes for human proliferating cell nuclear antigen (PCNA). Methods By using 3 epitope prediction databases which including MHC binder ( BIMAS and SYFPEITHI databases) and proteasome cutting site ( PAProC databases), the epitopes of PCNA were predicted by analyzing several parameters and methods. Results After comprehensive analysis,we have obtained two possible HLA-A0201 restricted CTL epitopes SMSAD-VPLV (228-236, score 447 633. 595 ) and LINEACWDI ( 22-28, score 127 966.779);two HLA-A24 re-stricted CTL epitopos DYEMKLMDL ( 113-121, score 563 994.9 ) and EFARICRDL ( 143-151, score 40 540.7);two HLA-A1101 restricted CTL epitopes LTSMSKILK (72-80, score 1334. 2680 ) and DVPLV-VEYK (232-240,score 736.9236). Conclusion The multi-parameter epitope prediction method is feasi-ble for cytotoxic T lymphocyte epitope prediction.     

疝环充填式无张力疝修补术治疗腹股沟疝(附102例报告)

目的：探讨疝环充填式无张力疝修补术治疗腹股沟疝的临床效果和应用价值。方法：回顾性总结我院2000年2月－2001年12月行疝环充填式无张力疝修补术治疗腹股疝102例，共108例次。结果：本组无手术死亡，无切口感染，均痊愈出院。9例出现阴囊水肿，1例荨麻疹病人出现过敏反应，2例出现伤口局部疼痛。随访1－22个月，随访率83．3％，无一例复发。结论：疝环充填式无张力疝修补术是一种符合重复解剖的先进手术方法，具有手术简单、安全、创伤小、恢复快、痛苦少、复发率低、适应证广等优点，适用于各型成人腹股沟疝，尤其适合于年老体弱、巨大疝、复发疝病人，值得推广应用。     

内源性血管紧张素Ⅱ在梗阻性黄疸大鼠应激性溃疡中的作用

内源性血管紧张素Ⅱ在梗阻性黄疸大鼠应激性溃疡中的作用杨乃众牟东成徐为杜如昱（北京医科大学人民医院外科，北京１０００４４）梗阻性黄疸（下称梗黄）术后应激性溃疡大出血的发生率和死亡率都很高，其原因尚不清楚。目前认为胃粘膜完整性的维持在于保证足够的粘膜血流...     

原发性骶前肿瘤56例临床诊治分析

目的探讨原发性骶前肿瘤的临床诊断及外科治疗方法。方法回顾性分析1980年1月至2015年7月间收治的56例原发性骶前肿瘤病人的临床资料,其中男性21例,女性35例,年龄23~68岁,平均36.9岁。病程10 d至7年。所有病人术前均经过直肠指检或影像学检查的诊断定位,其中经骶尾部入路25例,经腹入路24例,经腹经骶尾部联合入路7例。其中6例为腹腔镜下经腹切除。结果本组病人良性34例,恶性22例;肿瘤完整切除39例,部分切除10例,未切除7例。术后发生骶前脓肿2例,切口感染2例,直肠瘘1例。获得随访的病人中,肿瘤切除者,复发4例,死亡1例。肿瘤未切除者全部死亡。结论原发性骶前肿瘤缺乏特异性症状,直肠指检以及B超、CT、MRI等相关影像学检查对于其术前诊断及手术入路的选择有重要作用。手术治疗是骶前肿瘤的首选治疗方法。合理的手术入路选择以及对并发症的充分认识和准备是原发性骶前肿瘤治疗成功的关键。     

肿瘤Ki-67抗原HLA-A2限制性细胞毒性T淋巴细胞表位的鉴定

Objective To identify the HLA-A2-restricted CTL epitopes encoded by antigen segment of Ki-67 for preparation of tumor vaccines. Methods Two epitope prediction databases ( BIMAS and SYFPEITHI) were used to predict the epitopes of Ki-67, and 7 peptides were synthesized as the candi-dates. The prediction was evaluated by peptide-binding test. ELISPOT was used to inspect the IFN-γ secre-ted by CTLs in order to reflect the immunogenicity of the peptides. Results The purities of synthetic pep-tides and positive control peptide were all above 95%. Peptide-binding test displayed that LQGETQLLV (280-288) of Ki-67 could bind with HLA-A2 molecule more strongly. ELISPOT assay displayed that LQGETQLLV could induce activation of specific CTLs. Conclusion LQGETQLLV (280-288) might be the HLA-A2-restricted CTL epitope of Ki-67 antigen.     

改良式Appleby手术治疗胰体癌及胰胃二重癌2例报告

胰体尾癌常在出现腹、背部疼痛后或发现腹部包块等晚期表现时才能确诊 ,手术切除率较低 ,仅 5 %～ 10 % [1] ,预后较差。不能切除的原因主要为胰体尾周围大血管受浸润 ,如肝总动脉或腹腔动脉受浸润等。因此 ,受累动脉的切除可以提高胰体尾癌的切除率 ,并有可能延长患者术后的生