辛伐他汀对脂多糖诱导人THPL-1单核细胞MCP-1表达的影响

【目的】研究辛伐他汀对脂多糖（LPS）诱导人THP-1单核细胞表达单核细胞趋化蛋白-1（MCP1）的影响，探讨辛伐他汀的抗炎作用。【方法】体外培养人THP-1单核细胞，分为对照组、LPS组、辛伐他汀低、中、高浓度组，辛伐他汀三组加入不同浓度辛伐他汀预孵2h与LPS组均加入LPS刺激24h，应用Western blot与ELISA法分别检测各组细胞蛋白与培养基上清中MCP-1水平。【结果】LPS诱导人THP-1单核细胞MCP-1表达显著增加，辛伐他汀呈浓度依赖性显著抑制LPS诱导人THP-1单核细胞MCP-1表达。【结论】辛伐他汀能够抑制LPS诱导人THP-1单核细胞MCP-1表达，提示其具有抗炎作用。     

天晴复欣注射剂联合天晴甘平胶囊治疗慢性乙型肝炎40例

本我院近年来采用天晴复欣注射剂与天晴甘平胶囊联合治疗慢性乙型肝炎取得了较好的疗效,现将结果报告如下.     

医学检验技术专业本科毕业论文写作的培养

医学检验技术专业的培养目标,医学检验技术专业学生撰写毕业论文很有必要性。该文总结了皖南医学院检验学院培养医学检验技术专业本科学生撰写毕业论文的具体措施,取得的成绩,以及做得不足的地方。     

健脑安对血管性痴呆模型大鼠海马胆碱乙酰转移酶和突触素表达的影响

目的观察健脑安对血管性疾呆(VD)大鼠海马胆碱乙酰转移酶(ChAT)和突触素(Syn)表达的影响。方法健脑安口服给药治疗VD大鼠1月后采用免疫组化技术观测大鼠海马ChAT和Syn表达变化,与都可喜(Duxil)对照。结果①各组大鼠海马ChAT和Syn阳性表达面积比较如下,健脑安组(2 980±786.53)/(2 948.0±811.92)μm2和都可喜组(3 116.6±808.25)/(3 086.5±862.48)μm2均明显高于模型组(1 004±464.4)/(1 704±671.74)μm2)P值均<0.01)。②ChAT免疫组化染色:健脑安组和都可喜组大鼠海马ChAT阳性神经细胞排列较规则,胞浆ChAT阳性染色较明显,可见阳性染色神经纤维,较密集。③Syn免疫组化染色:健脑安组和都可喜组大鼠海马神经细胞周围Syn阳性颗粒密集、染色深,可见少量胶质细胞。健脑安组和都可喜组比较未见有明显差异。结论健脑安具有显著增加VD大鼠海马ChAT和Syn表达的作用,这可能是其改善痴呆认知和记忆障碍的作用机制。     

食欲素A对慢性间歇缺氧大鼠呼吸活动及颏舌肌肌电的影响

目的 探讨食欲素A(Orexin A)对慢性间歇缺氧(CIH)大鼠呼吸活动及颏舌肌肌电的影响。方法 选取成年雄性SD大鼠32只,分别建立对照组(大鼠置于常压常氧动物仓中,连续处理4周)及CIH组(建立CIH大鼠模型,慢性间歇低氧处理,交替给予氮气及空气,每天8 h,连续处理4周)。分析对照组及CIH组大鼠的呼吸活动及颏舌肌肌电(心率、平均动脉压、呼吸频率及颏舌肌肌电); 50μmol/L,100μmol/L及200μmol/L三种剂量Orexin A对CIH大鼠的呼吸活动及颏舌肌肌电的影响; Orexin A受体拮抗剂对CIH大鼠呼吸活动及颏舌肌肌电的影响及内源性Orexin神经损毁大鼠呼吸活动及颏舌肌肌电的影响。结果 与对照组大鼠相比,CIH组的颏舌肌紧张性活动、呼吸性相关性活动明显较低(P 0. 05);两组的呼吸频率对比无统计学差异(P 0. 05)。给药前,三组大鼠的颏舌肌紧张性活动肌电、呼吸性相关性活动肌电、呼吸频率、心率、平均动脉压比较,差异无统计学意义(P 0. 05);给药后,颏舌肌紧张性活动肌电及呼吸性相关性活动肌电均明显上升,且随着Orexin A剂量的增加,以上指标明显增加(P 0. 05)。给药后,OX1R组与OX2R组的颏舌肌紧张性活动肌电与呼吸性相关性活动肌电均明显降低,两组的呼吸频率、心率、平均与给药前比较,差异无统计学意义(P 0. 05)。Orexin神经损毁后,颏舌肌紧张性活动肌电与呼吸性相关性活动肌电均明显降低(P0. 05);呼吸频率、心率及平均动脉压比较,差异无统计学意义(P 0. 05)。结论 外源性Orexin A会增强CIH大鼠的颏舌肌活动,且随着Orexin A剂量的增加,其对CIH大鼠的颏舌肌活动增强,损毁或抑制CIH大鼠Orexin神经,会降低颏舌肌活动;增加或抑制Orexin A对CIH大鼠的呼吸活动影响较小。     

肾素-血管紧张素系统的回顾

肾素-血管紧张素系统(RAS)新成员的发现,改变了既往认为只有血管紧张素转化酶(ACE)催化生成的血管紧张素(Ang)Ⅱ才产生致病作用的观点,证明肾素与前肾素-肾素受体(RPR)的结合及AngⅣ与胰岛素调控氨基肽酶受体(IRAP)的结合同样也会产生致病作用。新发现的ACE2催化Ang-(1-7)和Ang-(1-9)的生成,Ang-(1-7)与Mas受体和Ang-(1-9)与AT2受体结合之后,可产生心血管保护作用,但RAS的致病作用仍然占优势。RAS新成员的发现,对认识RAS抑制剂的临床研究结果提供了新的理论依据。     

Association of IL33/ST2 Signal Pathway Gene Polymorphisms with Myocardial Infarction in a Chinese Han Population

This study investigated the relationship between IL-33/ST2 signal pathway gene polymorphisms and myocardial infarction(MI) in Han Chinese. A case-control association analysis was performed on a total of 490 MI patients(MI group) and 929 normal subjects(NC group). Sequenom Mass Array and Taqman genotyping technique were used to analyze the tag single nucleotide polymorphisms(SNPs) in the genes encoding IL-33, ST2, and IL-1Ra P(rs11792633, rs1041973 and rs4624606). The results showed that the frequencies of rs4624606 genotypes AA, TT, AT were 0.031, 0.647, 0.322 in MI group and 0.026, 0.712, 0.263 in NC group, and the allele frequencies of A and T were 0.192, 0.808 in MI group and 0.157, 0.843 in NC group. There were significant differences in rs4624606 genotypes and allele frequencies between MI group and NC group(P<0.05). For rs11792633, the allele frequencies of C and T were 0.45, 0.55 in MI group and 0.454, 0.546 in NC group with no significant differences found between the two groups. Compared with genotype CC+TC, rs11792633 genotype TT had an increased risk of hypertension(P<0.05). However, there were no significant differences in the frequencies of rs11792633 genotypes between the two groups. No significant differences were noted in the frequencies of rs1041973 genotype and allele between the two groups. Logistic regression analysis showed that rs4624606 genotypes AT and AA+AT were both significantly associated with MI(AT: OR=1.325, P=0.029, 95% CI=1.03–1.705; AA+AT: OR=1.316, P=0.028, 95% CI=1.03–1.681) after factors such as age, gender, smoking, drinking, body mass index(BMI), triglyceride(TG) and cholesterol were adjusted. Those carrying rs4624606 genotype AT or AA+AT had an increased risk of MI. No associations were found between the polymorphisms of the other two loci with MI. It was concluded that, in the IL33/ST2 signal pathway, the A allele of rs4624606 polymorphism of IL-1Ra P gene is a potential independent risk factor for MI, and the genotypes AA+AT and AT are associated with the incidence of MI.