基于GEO数据库的IgA肾病基因的筛选和生物信息学分析

目的通过生物信息学分析鉴定IgA肾病的生物标志物,旨在阐明IgA肾病疾病进展的可能分子机制。方法从GEO数据库下载包含IgA肾病患者和健康对照的微阵列数据集GSE104948、GSE93798和GSE37460数据集,并使用limma R包进行分析,以获得差异表达基因。然后进行GO分析和KEGG通路富集分析,运用STRING和Cytoscape软件构建蛋白互作网络以阐明IgA肾病的分子机制。结果应用limma R包分析鉴定出30个DEG,包括16个上调基因,14个下调基因。FN1和COL1A2等上调基因主要参与细胞外基质受体互作及PI3K-Akt信号通路。IgA肾病中ALB基因显著下调。FN1,COL1A2,ALB和FABP1基因被筛选为枢纽基因。结论FN1、COL1A2、ALB和FABP基因可能在IgA肾病的发展中起重要作用,并可能作为诊断和治疗IgA肾病的潜在分子靶标。     

Nucleostemin特异性RNA干扰对HeLa细胞体内外增殖能力的影响

目的：检测肿瘤细胞Nucleostemin（NS）的表达，研究NS特异性RNA干扰对HeLa细胞体内外增殖的影响。方法：提取6种肿瘤细胞总RNA，用 RT-PCR和Northern blot方法检测NS的表达。用NS特异性siRNA表达载体转染HeLa细胞，观察转染的HeLa细胞（简称NS-siRNA-HeLa细胞）体内外增殖的变化。结果：6种肿瘤细胞中NS明显高表达。体外培养的NS-siRNA-HeLa细胞中NS表达显著低于对照组，G0/G1期细胞百分率显著升高。体内致瘤实验显示，NS-siRNA-HeLa细胞在裸鼠体内增殖显著低于对照组。结论： NS在肿瘤细胞中高表达具有普遍性。NS特异性RNA干扰使HeLa细胞进入S期受阻，并可明显降低HeLa细胞体内外的增殖能力。     

Transfer of germinal vesicle to ooplasm of young mice could not rescue ageing-associated chromosome misalignment in meiosis of oocytes from aged mice

BACKGROUD: Transferring a germinal vesicle (GV) from an aged woman's oocyte into ooplasm from a younger woman has been proposed as a possible way to overcome the problem of age-related decline in female fertility. Here we assessed this possibility by determining whether ooplasts derived from young mice could rescue ageing-associated chromosome misalignment in meiosis of oocytes from aged mice. METHODS: Three groups of reconstructed oocytes, young GV-young cytoplast (group YY), aged GV-young cytoplast (group AY), and young GV-aged cytoplast (group YA), were created by micromanipulation and electrofusion. RESULTS: Nuclear transplantation was successful in 89.8-94.4% of GV-ooplast complexes, and maturation rate of the reconstructed oocytes was 93.5-97.9%. Confocal microscopy analysis showed a significantly higher rate (49.2%) of chromosome misalignment in ageing mice than in young mice (16.9%), and 57.1% of oocytes in group AY exhibited chromosome misalignment, while the abnormality rate in groups YY and YA was 16.3 and 16.7% respectively. Calcium imaging showed that the three groups of reconstructed oocytes exhibited a similar pattern of calcium oscillations upon stimulation with bovine sperm extracts. Fertilization rate and developmental capacity to 2-cell embryos were also similar among the three groups of oocytes. CONCLUSIONS: Our findings suggest that: (i) the ooplasm from young mice could not rescue ageing-associated chromosome misalignment in meiosis of GV from aged mice; and (ii) behaviour of chromosome alignment over metaphase spindle is predominantly determined by GV material.     

氧等离子体硫化法制作可变形聚二甲基硅氧烷薄膜培养基底

发展了氧等离子体硫化法制作可变形聚二甲基硅氧烷薄膜培养基底的技术。与传统的加热硫化法相比，该法制作的基底表面具有亲水性，细胞的黏附、铺展以及基底皱褶变形的发展均非常迅速。此外，观察了细胞松弛素D处理过程中基底皱褶的变化，结果表明这种技术具有较高的时间分辨率。     

Establishment and characterization of a cytotrophoblast cell line from normal placenta of human origin

A cell line has been established from human placentae at thefirst trimester of normal pregnancy. The cell line was obtainedby culture of purified cytotrophoblast cells in serum-free mediumsupplemented with epidermal growth factor, insulin, dexamethasoneand 0.1% bovine serum albumin. The cells can be subculturedfor >30 passages in one to three splits. All the cells weremononuclear epithelial-like cells positive to cytokeratin 18,gonadotrophin-releasing . hormone (GnRH), neuropeptide Y, neurotensin,leucine-enkephalin, dopamine and 5-hydroxytryptamine inununo-cytochemicalstaining. The cells secreted GnRH, progesterone and oestradiol(in the presence of testosterone) but little human chorionicgonadotrophin and no -endorphin. The cell line showed humankaryotypes and had a population doubling time of 48 h in serum-freemedium. However, the cells would stop growing in the mediumcontaining fetal bovine serum. A normal cytotrophoblast cellline established in serum-free medium will be particularly usefulin the study of cytotrophoblast cell proliferation and differentiation.     

雪莲的研究进展

雪莲是我国珍稀名贵的中药材，具有很高的药用价值，应用前景广阔。雪莲生长环境恶劣，自然资源十分有限。由于近年来野生雪莲乱采滥挖现象严重，加之人工栽培困难，雪莲已被列为国家三级濒危物种而受到保护。综述雪莲的有效成分、药理作用、开发利用现状及解决雪莲资源短缺的途径，并对雪莲今后研究发展的方向进行了展望，以期更加有效地保护和合理开发利用雪莲资源、满足医药市场需求、保护西部生态环境和促进西部经济发展。     

环孢素对心脏移植大鼠外周血淋巴细胞多基因mRNA表达的影响

 目的 对大鼠心脏移植前后不同时间点外周血淋巴细胞mRNA差异表达的研究,探讨环孢素对免疫排斥反应过程中基因表达的影响.方法 心脏移植前后以15 mg·kg^-1·d^-1环孢素灌胃的大鼠,分别在移植前、移植后24,72 h,7,10,12d取血,提取总RNA,用mRNA差异显示法找出差异表达条带,克隆测序,用灰度扫描法计算差异表达基因的比密度。结果 对照组移植心脏存活率于移植后的10d内降至为0%,而此阶段环孢素组的移植心脏存活率始终保持在50％.移植后第10天,对照组与凋亡信号传导和线粒体蛋白构象重构与蛋白转运相关基因Ribosomal—12S和S25,MHC—Ⅲ(Hst-70)的表达水平显著高于移植前,与移植心脏存活率最低点同步.其2,3-bisphosphoglycerate和gig18基因表达水平的变化无统计学意义。环孢素组与细胞凋亡有关的gig18和Thbsl,以及T细胞受体(TCR)等基因的表达水平于移植后10 d内持续低于移植前,2,3-bisphosphoglycerate基因的表达水平于移植后24 h降低,只有钠通道β-1基因的表达水平于移植后10 d内高于移植前。结论 环孢素可负调心脏移植后大鼠外周血淋巴细胞凋亡相关基因gig18和Thbsl,正调钠通道基因的转录。     

生化过程工程与中药现代化

在生化工程20余年的研究工作基础上。结合中药现代化开展了一些技术、材料与设备的研究开发工作。研究植物细胞、组织、器官大规模培养增殖技术，应用于细胞代谢产物、生物转化和人工育种及发根，实现了工厂化生产，并成功研制多种类型的生物反应器；在原有化学工程提取分离技术的基础上，发展了反应分离耦合、微波辅助提取等新技术，实现了高效浸出，并且节能、节水；此外，又发展了包括反胶团萃取、一步三相萃取青霉素、泡沫分级分离、膜分离、高速逆流色谱分离纯化等新技术。高速逆流色谱技术是一种没有固相载体的液一液多级逆流萃取技术，避免了不可逆吸附，已成功用于多种天然产物的分析和分离，作为研究中药指纹图谱的新方法具有很好的精密度和重现性。高效液相色谱、气相色谱、毛细管电泳、质谱等技术在指纹图谱研究中发挥了重要作用。与此同时，还研制了多种分离介质，并在药物的修饰与包埋方面做了大量工作。另外，在海洋药物研究领域，创造了连续培养连续采收的新流程、新技术．进行了转基因藻的培养。用于生产基因工程产品。     

Selective loss of basal forebrain cholinergic neurons in APP770 transgenic mice

Objective To determine whether cholinergic neurons in the basal nucleus magnocellularis (N BM) and the medial septum are affected in transgenic mice overexpressing human a myloid precursor protein 770 (APP₇₇₀).
Methods Eight age groups, from 3 months old to 10 months old, of either heterozygous tra nsgenic or non-transgenic mice were used for choline acetyltransferase (ChAT) s taining using immunohistochemistry. The number of ChAT-positive neurons was co unted on the MCID Image Analysis System. Neurons in the cerebral cortex and are a CA1 of hippocampus were also stained with cresyl violet and counted using opti cal dissector technique.
Results There is no change in the number of forebrain cholinergic neurons in the transge nic mice up to 9 months of age. A loss of these cholinergic neurons starts in 9 months old transgenic mice, with a further decrease in the number of NBM and me dial septum neurons in 10-month-old transgenic mice. On the other hand, the n umber of neurons in the cerebral cortex and hippocampal area CA1 remained unchanged.
Conclusion These results demonstrate a selective loss of basal forebrain cholinergic neuron s in APP₇₇₀ transgenic mice.     

异丙酚在人工流产术中的临床效果观察

目的:观察异丙酚在人工流产术中麻醉效果、宫颈松弛程度、阴道出血量、人工流产综合征及术中不良反应。方法:选择对象200例,分观察组、对照组各100例,观察组术前静脉注射异丙酚,对照组术前未给予任何药物,手术方法两组相同。结果:观察组镇痛率为:100％,无人工流产综合征发生;宫颈口松弛程度和术中不良反应,两组有显著性差异(P<0.01);两组术中出血量比较无统计学意义(P>O.05)。结论:异丙酚用于人工流产术镇痛效果较好,可避免人工流产综合征的发生,减少术中不良反应,缩短手术时间,对子宫收缩无影响,可以广泛应用于无痛人工流产术。