重庆市新结防模式下MDR-TB患者治疗现状及经济负担调查研究

本研究评估重庆市在新结防模式下MDR-TB患者的治疗现状及经济负担,为MDR-TB控制提供决策依据。研究表明患者接受正规治疗的比例较高,医保覆盖率也较高,但也还存在相关卫生服务可及性差、治疗副反应普遍、患者经济负担较重等问题。后续应加强重点人群和弱势人群MDR-TB的筛查,提升相关卫生服务的可及性,同时需要加强对患者的经济支持。     

医学期刊编辑素质刍议

本文着重阐述了医学期刊编辑应具备最基本、最重要的三大素质：政治素质、业务素质和创新素质。特别强调医学期刊编辑要有创新素质，只有创新，才有发展和进步。刻苦学习，不断进取，努力使自己成为医学家、编辑家和企业家。     

New syndrome: Mother and son with hypertelorism,downslanting palpebral fissures,malar hypoplasia,and apparently low-set ears associated with joint and scrotal anomalies

We report on a mother and son with a similar syndrome of hypertelorism and telecanthus, epicanthal folds, downslanting palpebral fissures, ptosis, broad nasal bridge, malar hypoplasia, thin upper lip, smooth philtrum, and apparently low-set prominent ears. The son also has a hypoplastic shawl scrotum, cryptorchidism, and genu valgum. His language development was delayed at 18 months, but subsequently improved and was normal at age 3. The mother has the additional findings of marked cubitus valgus, hyperextensible joints, dull normal intelligence and a bleeding diathesis. This pattern of multiple congenital anomalies may represent a new syndrome.     

Connective tissue repair in circumferential periodontal defects in dogs following use of a biodegradable membrane

A biodegradable polylactic acid membrane was evaluated for its ability to enhance periodontal regeneration. In 8 Labrador dogs, circumferential defects were created around the maxillary 1st premolars. The defects were combination "1-wall vertical and horizontal". A membrane was adjusted to cover the defect on one side of the jaw, while the contralateral tooth served as control without membrane. After 3 months, healing was evaluated histologically. The results demonstrated that the amount of connective tissue repair with newly formed cementum approximated 50% of the defect height for both test and control groups. Thus, no advantage to the use of membrane was found, which is contradictory to the findings of our previous dog study using the same biodegradable membrane. The possible reasons for this difference in results are discussed relative to suggested mechanisms for membrane effects in periodontal regeneration.     

家兔骨生长诱导修复腭裂骨缺损的实验研究

目的　研究评价牛骨形成蛋白 (b BMP) -白蛋白 -成纤维细胞生长因子 (b FGF)复合材料修复腭裂骨缺损的可行性。方法　建立家兔腭骨缺损模型 ,将固化的 b BMP-白蛋白 - b FGF复合材料植入骨缺损区 ,通过其骨诱导作用 ,诱导新骨生成 ,修复骨缺损。经 X线、组织学观察和钙含量测定。结果　 b BMP-白蛋白 - b FGF有很强的骨诱导作用 ,可以诱导骨形成 ,实验组与对照组 X线骨修复分数评估分别为 12分 ,0分 ,组织学检查实验组骨修复完成 ,而对照组无骨修复 ,钙含量测定也有显著性差异。。结论　 b BMP-白蛋白 - b FGF复合材料可诱导修复腭骨缺损 ,有良好的临床应用前景。     

新型短节段内固定器治疗腰椎滑脱症的临床应用

目的：探讨新型短节段内固定器治疗腰椎滑脱症的临床应用价值。方法：对１８例腰椎滑脱的脊椎应用新型内固定器进行复位、固定,恢复脊椎的正常解剖结构,并自髂嵴后部取骨行横突间植骨融合。结果：术后随访１２￣２４个月,平均１８个月,滑脱复位率达９０％,４￣５月后达到骨性愈合,无螺钉松动,拨出,断裂及脊住骨滑脱现象。结论：新型短节段内固定器治疗腰椎滑脱症复位率高,固定螺钉松动,拨出,断裂及再滑脱等并发症少,疗效     

抗人胸腺细胞单克隆抗体2G_2B_2和2G_2C_2的制备及特性鉴定

用杂交瘤技术制备２株单克隆抗体（单抗）２Ｇ２Ｂ２、２Ｇ２Ｃ２。经研究发现,它们主要与胸腺细胞、脐血及ＰＨＡ激活的外周血单个核细胞等反应;免疫组化显示其主要作用于皮质胸腺细胞;与造血系统中分化早期的瘤细胞、骨髓瘤细胞等反应阳性率较高。生物学特性鉴定结果提示,单抗均属免疫球蛋白ＩｇＧ１亚类,腹水效价达１：１２８０００,所识别抗原分子量为４５／４６ＫＤａ,无抗原调变作用。故２Ｇ２Ｂ２、２Ｇ２Ｃ２是类似ＯＫＴ１０的抗ＣＤ３８单抗,但竞争抑制结果表明三者分别作用于不同的抗原决定簇,即２Ｇ２Ｂ２、２Ｇ２Ｃ２是两个抗ＣＤ３８新单抗。     

中药有效成分研究与复方新药开发

１０００００首方剂是开发中药复方新药的宝库。１２７７２种中药及其复方中的有效成分是开发中药复方新药的基础。逆向思维、利用文献、研究有效成分动态变化和综合作用是开发中药复方新药的几种途径     

登革病毒大片段NS3基因扩增的方法探讨

目的：探索一种快速、实用的扩增登革病毒大片段基因的方法。方法：用登革２ 型新几内亚 Ｃ 株（ Ｄ Ｖ２ , Ｎ Ｇ Ｃ 株）的核酸（ Ｒ Ｎ Ａ） 为模板,研究了在不同条件下（ 病毒和其变性液的量以及ｃ Ｄ Ｎ Ａ 的浓度等） 通过逆转录聚合酶链式反应技术扩增大片段 Ｎ Ｓ３ 基因,并对扩增的目的基因用巢式 Ｐ Ｃ Ｒ 进行了鉴定。结果：用大量的或小量的登革病毒培养上清中提取 Ｒ Ｎ Ａ 的方法在一定条件下均可扩增出大片段 Ｎ Ｓ３ 基因。结论：小量登革病毒提取 Ｒ Ｎ Ａ 扩增大片段 Ｎ Ｓ３ 基因法较大量登革病毒提取 Ｒ Ｎ Ａ 扩增大片段 Ｎ Ｓ３ 基因快速、实用。     

Anti-invasion drugs

Summary Most of the pharmaceuticals in clinical practice today for treatment of breast and other cancers are cytotoxic or cytostatic inhibitors of tumor growth. While this type of drug has found its place, along with surgery and radiotherapy, in treatment of disease, the breast cancer death rate has not decreased. This appears to be the result of rising incidence, resistance to therapy, and metastasis of the disease. Since distant metastasis (usually indicated by lymph node involvement) of breast cancer is related only indirectly to tumor size, it would appear that a concerted effort should be made to discover drugs which directly interfere with this complex process. Metastasis appears to depend upon tumor cell motility, dedifferen-tiation, local invasion, and angiogenesis. Significant progress has been recently made in the creation of new animal models of metastasis and in identifying several new drugs which may be suitable for clinical inhibition of this process. This article reviews current findings on anti-invasion/metastasis drugs with a focus on breast cancer.Presented at the symposium "New Approaches in the Therapy of Breast Cancer", Georgetown University Medical Center, Washington DC, October 1994, generously supported by an education grant from Bristol-Myers Squibb.