Construction of Antisense MT1-MMP Vector and Its Inhibitory Effects on Invasion of Human Ovarian Cancer Cells

Themetastaticspreadofcancercellsfromaprimarytumortodistantsitesinthebodyisre sponsibleformostcancerpatientmorbidityandmortality.Duringtumormetastasis,thedegrada tionofexteacellularmatrix(ECM)isakeystep.Membrane type1matrixmetalloproteinase(MT1MMP/MMP14)isanewlydiscoveredenzyme,whichplaysacrucialroleintumormetastasisbydirectlybreakingthematrixbyproteolysisinthepericellularspaceandindirectlyactivatingpro MMP2[1].Atpresent,itiselusivewhetherendog enousexpressionofMT1MMPinhumanovariancance…     

Inhibition of Invasion and Up-regulation of E-cadherin Expression in Human Malignant Melanoma Cell Line A375 by（-）-Epigallocatechin-3-gallate

The inhibitory effects of （-）-epigallocatechin-3-gallate （EGCG） on the invasion of human malignant melanoma cell line A375 and the possible molecular mechanisms of this effect were investiaged. A375 cells were pretreated with 20 μg/mL EGCG for 24, 48 and 72 h respectively and the E-cadherin expression was detected by Western blot analysis. A375 cells were also pretreated with different concentrations of EGCG （1, 5, 10 and 20 μg/mL） for 72 h and the expression of E-cadherin was measured by RT-PCR. The adhesion and invasion of A375 cells were tested by cell-matrigel adhesion assay and matrigel invasion assay respectively. The results showed that EGCG could significantly up-regulate the expression of E-cadherin time-and concentration-dependently （both P〈0.05）. Statistical analysis showed that A375 cells invasion was inhibited by EGCG and correlated with the up-regulation of E-cadherin expression. It was suggested that EGCG strongly inhibited invasion of A375 cells, and the inhibition mechanism was possibly associated with the up-regulation of E-cadherin expression.     

入侵检测技术在医院中的应用

介绍入侵检测技术的原理、发展和目前存在的缺陷。介绍入侵检测技术和其它安全技术的联合应用为医院信息系统的运行提供有效保障。     

坐骨神经痛的多针刺法

坐骨神经痛是指以沿坐骨神经通路疼痛为主要症状的综合征,是各种原因引起坐骨神经受压而出现的炎性病变.中医称其为"腰腿痛"、"痹证",其病因病机为:腰部闪挫、劳损、外伤等,损伤筋脉,导致气血瘀滞,不通则痛;久居湿地,或涉水冒雨,汗出当风,风寒湿邪入侵,痹阻腰腿部;或湿热邪气侵淫、湿浊郁久化热及机体内蕴湿热,流注膀胱经,均可导致腰腿痛.在《灵枢*经脉》中论述足太阳膀胱经的病证时有"腰似折,髀不可以屈,月国如结,踹如裂"的记载,形象地描述了本病的临床表现.     

RNA干扰与恶性肿瘤治疗

RNA干扰（RNA interference,RNAi）是指在进化中高度保守的、由双链RNA（double-stranded RNA,dsRNA）诱发的、同源mRNA高效特异性降解的现象。它已在许多不同种属的生物体中被发现,并在生物体细胞之间进行传递,具有抵抗病毒入侵和维持基因组稳定的作用。由于使用RNAi技术可以特异性剔除或关闭特定基因表达．     

基于免疫学原理的混合入侵检测系统的设计与实现

讨论了基于免疫学的入侵检测系统的架构和各模块的功能,重点研究了本文提出的基于改造开发源代码的入侵检测系统Snort的免疫检测器模块的设计,以及免疫检测器的检测流程.以KDDCup99数据集为样本,运用本文设计的基于免疫学原理的混合入侵检测系统进行了入侵检测实验,对全部18种攻击类型行为的平均检测率为64.94%,检测结果令人鼓舞.     

千兆线速入侵检测系统的设计与实现

随着千兆网络开始在国内大规模普及,对千兆线速入侵检测系统等高性能网络安全产品的需求日益迫切.本文基于IXP2400、pp1200、TCAM芯片和CPCI 2.16技术开发了千兆线速入侵检测系统硬件平台,并基于该平台设计并实现了一个千兆线速级入侵检测系统.该入侵检测系统较之用网络处理器(NP)和专用集成电路(ASIC)技术实现的千兆入侵检测系统具有结构开放、灵活,综合计算能力强,可靠性高等优势.     

基于智能网格模型的入侵检测系统

针对网格平台的异构性、动态性和分布性等特点,结合智能Agent技术,提出了一种智能网格模型(Intelligent and Corporative Grid Model,ICGM).ICGM具有自组织、自管理和智能协作等特性,能很好地适应复杂网格环境,有效地屏蔽网格平台中的不利特性.在分析网格中入侵检测需求后,设计了基于ICGM的网格入侵检测系统(IDS based on ICGM,gIDS).gIDS具有较好的智能性、灵活性和扩展性,能有效地检测网格中的入侵行为,确保网格平台的安全.对比实验表明,基于智能网络模型的gIDS在检测率、误报率及检测速度方面具有良好的性能,也体现出智能Agent技术与网格技术的整合能从一定程度上解决网格自身存在的问题.     

Vascular Invasion as An Independent Prognostic Indicator in Radically Resected Non-small Cell Lung Cancer

Objective： A retrospective study was performed to analyze the impact of vascular invasion on prognosis in a series of radically resected non-small cell lung cancer （NSCLC） and the subgroup of T1-4 nodal negative NSCLC patients. Methods： A total of 259 NSCLC patients who had undergone radical resection were entered into this study. Detailed clinical data including five-year survival were obtained for all the patients. The tumors were reviewed for the presence or absence of vascular invasion. Fisher＇s exact tests were used to assess the relationship between vascular invasion and other clinicopathological variables. Survival time was defined as the interval from the date of operation to either death from lung cancer or the last follow-up. Univariate analysis of survival curve was performed by the Kaplan-Meier method using the Log rank test. Multivariate survival analysis was carried out by Cox regression. P〈0.05 was considered statistically significant. Results： In 259 patients, 33 cases were diagnosed as having vascular invasion. The overall 5-year survival was 37.5%. Patients with vascular invasion had a median survival of 20 months compared with 43 months for those without vascular invasion （P〈0.01）. Multivariate analysis indicated that vascular invasion was a significant independent prognostic predictor for shortened cancer-related survival in the patients. The relative risk for cancer-related survival was 2.2-fold greater in patients with vascular invasion （95% CI： 1.45-3.32）. Subgroup analysis revealed that patients with vascular invasion had a 5-year survival of 11.1% compared with 57.1% for those without vascular invasion in the resected lung cancer patients at T1-4N0M0 （P=0.002）. Conclusion： Vascular invasion can serve as an independent prognostic factor in radically resected NSCLC.