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41.
由T细胞株(Molt 4)产生的巨噬细胞激活因子(MAF)用阴离子交换和凝胶过滤色谱纯化。纯化的MAF进行PAGE出现一条带,进行SDS-PAGE证实其为单亚基,分子量为60000;IEP表明其等电点在pH3.5~4.5之间。 相似文献
42.
43.
用正常人的外周血粒细胞免疫Balb/c小鼠,取免疫脾细胞与小鼠骨髓瘤细胞系Sp2/0融合,用间接免疫荧光法及扁桃腺冰冻切片免疫过氧化物酶染色技术,从2次细胞融合中筛选并经克隆化得到一批稳定分泌抗粒细胞单抗的杂交瘤细胞系。对其中4株(FPMNI-FPMN4)杂交瘤细胞系所分泌的单抗作了系统的鉴定,证实它们是粒细胞特异性的单抗。 相似文献
44.
Prof. Dr. W. Jelkmann G. Wiedemann 《Journal of molecular medicine (Berlin, Germany)》1990,68(8):403-407
Summary The question as to whether the serum concentration of erythropoietin is relatively high for the degree of anemia in patients with erythrocytic hypoplasia has regained interest, since recombinant human-like erythropoietin has become available as a drug for replacement therapy. We have compared the concentration of serum immunoreactive erythropoietin in nonrenal anemic patients with erythrocytic hypoplasia (22 cases) or active erythropoiesis (82 cases). In both groups a negative correlation was determined between the blood hemoglobin concentration and the logarithm of the erythropoietin concentration. However, the two regression lines were not identical, and the serum erythropoietin concentration was significantly higher for the degree of anemia in the patients with erythrocytic hypoplasia. Additional measurements in four patients suffering from acute leukemia with marrow failure showed that the erythropoietin concentration decreased towards the values observed in anemic patients with active erythropoiesis when the erythron recovered in the early phase of complete remission. These data support the idea that, independent of the O2 offer, the proliferating erythrocytic progenitors by negative feedback lower the blood level of erythropoietin.Abbreviations Epo
Erythropoietin
- AML
acute myelogenous leukemia
- CML
chronic myelogenous leukemia
- ALL
acute lymphoblastic leukemia
- CLL
chronic lymphatic leukemia
- PBS
phosphate buffered saline
- BSA
bovine serum albumin
- Hb
hemoglobin
- DNA
desoxyribonucleic acid
A preliminary report of these studies was given at the XIIth International Symposium on Structure and Function of Erythroid Cells in Berlin, August 1989 [18] 相似文献
45.
目的:试图用计算机设计并筛选出血管内皮生长因子(VEGF)的高效反义核酸;用实验方法研究VEGF反义核酸对K562细胞增殖的影响。方法:用RNAstructure(version3.7)软件,选择总自由能(overall△G37)相对低的反义核酸,共计7条,长度18-20核苷酸,全硫代修饰;细胞培养72h,采用台盼蓝拒染法观察存活细胞,用ELISA法检测培养液中VEGF蛋白水平,分析反义核酸对K562细胞的作用。结果:筛选出6条反义药物对K562细胞生长有明显抑制作用,均优于阳性对照组(X2)。与随机对照组(X1)相比,最优序列X7组细胞生长抑制率达31.9%、培养液中VEGF蛋白表达抑制率达51.4%,overall△G37与反义药物活性密切相关(r=0.887,P<0.01)。结论:计算机辅助设计有助于获得更好反义药物,VEGF反义药物可抑制K562细胞生长及VEGF蛋白表达,内源性VEGF蛋白具有促进K562细胞增殖的功能。 相似文献
46.
广东省人群中人嗜T细胞病毒Ⅰ型感染的血清流行病学调查及其与人类疾病的关系 总被引:7,自引:0,他引:7
收集广东省各类人群血清标本2224份,用间接免疫荧光法检测人类嗜T细胞病毒Ⅰ型(HTLV-1)抗体,结果:HTLV-1抗体阳性35份,总阳性率为1.57%;其中健康人群血清1810份,抗体阳性23份,阳性率为127%;献血员血清248份,抗体阳性1份,阳性率040%;白血病患者血清109份,阳性8份,阳性率734%,与健康人群比较,有非常显著性差异(P<0005);神经系统疾病患者血清57份,阳性3份,阳性率526%。 相似文献
47.
Functional analysis of PTPN11/SHP-2 mutants identified in Noonan syndrome and childhood leukemia 总被引:3,自引:0,他引:3
Niihori T Aoki Y Ohashi H Kurosawa K Kondoh T Ishikiriyama S Kawame H Kamasaki H Yamanaka T Takada F Nishio K Sakurai M Tamai H Nagashima T Suzuki Y Kure S Fujii K Imaizumi M Matsubara Y 《Journal of human genetics》2005,50(4):192-202
Noonan syndrome (NS) is characterized by short stature, characteristic facial features, and heart defects. Recently, missense mutations of PTPN11, the gene encoding protein tyrosine phosphatase (PTP) SHP-2, were identified in patients with NS. Further, somatic mutations in PTPN11 were detected in childhood leukemia. Recent studies showed that the phosphatase activities of five mutations identified in NS and juvenile myelomonocytic leukemia (JMML) were increased. However, the functional properties of the other mutations remain unidentified. In this study, in order to clarify the differences between the mutations identified in NS and leukemia, we examined the phosphatase activity of 14 mutants of SHP-2. We identified nine mutations, including a novel F71I mutation, in 16 of 41 NS patients and two mutations, including a novel G503V mutation, in three of 29 patients with leukemia. Immune complex phosphatase assays of individual mutants transfected in COS7 cells showed that ten mutants identified in NS and four mutants in leukemia showed 1.4-fold to 12.7-fold increased activation compared with wild-type SHP-2. These results suggest that the pathogenesis of NS and leukemia is associated with enhanced phosphatase activity of mutant SHP-2. A comparison of the phosphatase activity in each mutant and a review of previously reported cases showed that high phosphatase activity observed in mutations at codons 61, 71, 72, and 76 was significantly associated with leukemogenesis. 相似文献
48.
F. Porzsolt R. Janik G. Heil O. Brudler Ar. Raghavachar S. Scholz U. Papendick H. Heimpel 《Annals of hematology》1986,52(3):185-190
Summary Since the application of low doses of IFN-alpha is necessary to maintain remissions in Hairy Cell Leukemia (HCL) it is of interest whether peripheral blood mononuclear cells (MNC) of HCL patients can be induced in vitro to produce IFN-alpha. 9 patients suffering from advanced HCL were included in the study. The diagnoses were confirmed by characteristic findings in peripheral blood and bone marrow biopsies. For IFN treatment we initially used natural IFN-alpha (Bioferon) and switched later to recombinant IFN-alpha2 (Boehringer). MNC of 5 patients before IFN therapy and of 6 patients during IFN therapy (2–47 weeks) were induced by phythemagglutinin (PHA), Corynebacterium parvum (C.p.), and sendai virus (SV). PHA is known to induce IFN-gamma. Both, C.p. and SV induced IFN-alpha but no IFN-gamma in MNC of healthy controls and of IFN treated breast cancer patients. In HCL patients normal antiviral activities could be induced by PHA. Zero or only low antiviral activities could be induced in MNC from 9 patients tested on 22 occasions. It is concluded that MNC from patients with advanced HCL can be induced to produce IFN-gamma but no IFN-alpha. Since IFN-alpha but not IFN-gamma is produced by monocytes it is likely that reduced numbers of monocytes which were found in our HCL patients before and during IFN treatment account for the described deficiency of IFN-alpha production. 相似文献
49.
测定白血病脑脊液中SIL—2R,IL—6表达的临床意义 总被引:2,自引:0,他引:2
为了探讨急性淋巴细胞性白血病 (ALL)患者脑脊液中可溶性白介素 - 2受体 (SIL - 2R)、白介素 6(IL - 6)的表达及意义 ,采用双抗夹心ELISA法测定 3 0例ALL患者脑脊液 (CSF)中SIL - 2R ,IL - 6水平 ,并与 10名正常者进行对照 .结果 :ALL合并中枢神经系统白血病 (CNS -L)组较CNS -L已缓解及未合并CNS -L组二者水平显著升高 (P <0 0 1) ;CNS -L已缓解组二者水平接近对照组 (P >0 0 5 ) ;骨髓缓解及好转组二者水平低于治疗无效组 .结果表明 :监测二者水平变化有助于CNS -L早期诊断、估计预后、判断疗效 . 相似文献
50.
湖南省江华瑶族自治县14年白血病流行病学调查 总被引:1,自引:0,他引:1
目的:了解白血病在瑶族人群中的发病情况及危险因素。方法:调查1984-1997年全县白血病新发病例,计算其发病率并进行比较分析。结果:本县白血病年发病率为1.61/10万,明显低于全国白血病年均发病率,瑶族人群低于汉族人群。白血病的发病有时一空集积现象。结论:瑶族人群白血病率不高。 相似文献