组织·社会性课题学习法在临床护理管理现状中的应用

目的 探索和实践组织·社会性课题学习法在临床管理的现状,找出临床护理管理的缺陷,根据缺陷提出解决措施,从而提升优质护理服务.方法 2018年3月—2018年8月选取东北三省15家医院55名临床护理人员通过不同视角收集的图像资料,应用组织·社会性课题学习法分析临床护理管理中的缺陷并提出解决方案.结果 通过组织·社会性课题学习法的应用,多角度直观可视化分析了临床护理工作存在的缺陷并提出相应的解决方案.结论 组织·社会性课题学习法的应用可以促进护理管理创新,为管理者的工作方式提供全新的思路.     

Evaluation of mitogenome sequence concordance,heteroplasmy detection,and haplogrouping in a worldwide lineage study using the Precision ID mtDNA Whole Genome Panel

The emergence of Massively Parallel Sequencing technologies enabled the analysis of full mitochondrial (mt)DNA sequences from forensically relevant samples that have, so far, only been typed in the control region or its hypervariable segments. In this study, we evaluated the performance of a commercially available multiplex-PCR-based assay, the Precision ID mtDNA Whole Genome Panel (Thermo Fisher Scientific), for the amplification and sequencing of the entire mitochondrial genome (mitogenome) from even degraded forensic specimens. For this purpose, more than 500 samples from 24 different populations were selected to cover the vast majority of established superhaplogroups. These are known to harbor different signature sequence motifs corresponding to their phylogenetic background that could have an effect on primer binding and, thus, could limit a broad application of this molecular genetic tool. The selected samples derived from various forensically relevant tissue sources and were DNA extracted using different methods. We evaluated sequence concordance and heteroplasmy detection and compared the findings to conventional Sanger sequencing as well as an orthogonal MPS platform. We discuss advantages and limitations of this approach with respect to forensic genetic workflow and analytical requirements.     

高校图书馆推进ESI学科建设的情报服务

“双一流”导向下，推进ESI学科建设已经成为高校图书馆的服务核心。介绍了中国医科大学图书馆利用ESI、Incites和Web of Science数据库面向ESI学科建设的情报服务的实践过程，发现学科竞争力分析、潜力学科预测、挖掘学科领域热点与前沿、提供学科建设对策等ESI学科情报服务有助于决策人员优化学科布局、科研人员把握学科热点，助推ESI学科建设。     

Clinicopathological and prognostic significance of TINCR in caner: A meta-analysis

BackgroundIn a variety of cancers, the expression of TINCR is linked to the development, progression, metastasis, invasion, and prognosis of cancer. Our study is the first study used meta-analysis to explore the relationship between TINCR expression and cancer.MethodsBy looking up PubMed, Web of Science, CNKI database, we obtained 10 articles for analysis. The statistical analysis was all calculated by Stata 15.1 software.ResultsWe found that the expression of TINCR was a risk factor to the size of the tumor (OR = 1.772, 95%CI: 1.246–2.520, P = 0.001). In univariate analysis, patients with high expression of TINCR had poor OS (pooled HR = 1.533, 95%CI: 1.025–2.294, P = 0.038). Similar result was also found in multivariate analysis In subgroup analysis (pooled HR = 1.610, 95%CI: 1.356–1.913, P = 0.000).We also found that over-expression of TINCR had poor OS in breast cancer (pooled HR = 1.582, 95%CI: 1.126–2.223, P = 0.008).ConclusionIn this study, we found that over-expression of TINCR may influence the tumor size and contribute to the poor prognosis of cancer.     

心脑宁胶囊治疗动脉粥样硬化性心血管疾病临床应用专家共识

本共识建立了成人动脉粥样硬化性心血管疾病(atherosclerotic cardiovascular disease,ASCVD)高危人群规范应用心脑宁胶囊以提升疗效的关键流程,概述了心脑宁胶囊的中医配伍理论、药学和药理研究特性,界定了心脑宁胶囊的精确适用人群、干预时机、病证疗效特点及优选应用方案,归纳了心脑宁胶囊的不良反应、使用禁忌、注意事项及非临床安全性等相关证据。本共识适合ASCVD相关专业领域的执业医师、执业中医医师和执业中西医结合医师使用。     

Novel modulation on myeloid-derived suppressor cells (MDSCs) by methionine encephalin (MENK)

The purpose of this study was to identify the modulatory effect of MENK on MDSCs and to investigate the relationship between this modulation and the expression of opioid receptors. Our results showed that MENK could inhibit the proliferation of MDSCs from both marine bone marrow and spleen. MENK also could promote the expression of opioid receptors MOR and DOR. MENK suppressed the PMN-MDSCs generated from splenocyte while up-regulated M-MDSCs. The stimulation of MENK increased the production of IL-4 secreted by MDSCs from slpenocytes. Our currently data indicated that MENK could suppress the accumulation of MDSCs in tumor-bearing mice via binding to and up-regulating expressions of subunits of opioid receptors. Therefore, it is concluded that MENK, through triggering opioid receptors could exert inhibiting modulation on MDSCs.     

Knockdown of long non-coding RNA HOTAIR inhibits malignant biological behaviors of human glioma cells via modulation of miR-326

Glioma is the most common and aggressive primary adult brain tumor. Long non-coding RNAs (lncRNAs) have important roles in a variety of biological properties of cancers. Here, we elucidated the function and the possible molecular mechanisms of lncRNA HOTAIR in human glioma U87 and U251 cell lines. Quantitative RT-PCR demonstrated that HOTAIR expression was up-regulated in glioma tissues and cell lines. Knockdown of HOTAIR exerted tumor-suppressive function in glioma cells. Further, HOTAIR was confirmed to be the target of miR-326 and miR-326 mediated the tumor-suppressive effects of HOTAIR knockdown on glioma cell lines. Moreover, over-expressed miR-326 reduced the FGF1 expression which played an oncogenic role in glioma by activating PI3K/AKT and MEK 1/2 pathways. In addition, the in vivo studies also supported the above findings. Taken together, knockdown of HOTAIR up-regulated miR-326 expression, and further inducing the decreased expression of FGF1, these results provided a comprehensive analysis of HOTAIR-miR-326-FGF1 axis in human glioma and provided a new potential therapeutic strategy for glioma treatment.