Effect of moxibustion on CRF and CRFR1 expressions in hypothalamus of TNBS-induced experimental colitis rats

Objective

To observe the effect of moxibustion on the protein and mRNA expressions of corticotropin-releasing factor (CRF) and corticotropin-releasing factor receptor 1 (CRFR1) in hypothalamus of trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis rats, and to explore the central mechanisms of moxibustion in improving visceral pain and the pain-related emotions in experimental colitis rats.

Methods

Thirty-six Sprague-Dawley (SD) rats were randomly divided into a normal group (NG), a model group (MG), a herb-partitioned moxibustion group (HPMG) and a sham herb-partitioned moxibustion group (SHPMG). Except the NG, rats in the remaining three groups all received TNBS enema to establish experimental colitis models. The HPMG received herb-partitioned moxibustion (HPM) at bilateral Tianshu (ST 25) and Qihai (CV 6) for intervention; for the SHPMG, the herbal cakes and moxa cones were only placed on the acupoints but not ignited; rats in the MG and NG were only fixed in the same way as those in the HPMG but did not receive any treatment. At the end of the intervention, the abdominal withdrawal reflex (AWR) score, the open field test (OFT) score and the elevated plus maze (EPM) score were observed to measure the changes in visceral pain and pain-related emotions of the rats. The enzyme-linked immunosorbent assay (ELISA) was used to examine the expressions of CRF and CRFR1 proteins in hypothalamus; the fluorescence-based quantitative polymerase chain reaction (PCR) was used to detect the expressions of CRF and CRFR1 mRNAs in hypothalamus.

Results

Compared with the NG, the AWR score increased significantly and the OFT and EPM scores dropped significantly in the MG (all P<0.05), and the expressions of hypothalamic CRF and CRFR1 proteins and mRNAs increased significantly (all P<0.01). Compared with the MG and SHPMG, the AWR score dropped significantly and the OFT and EPM scores increased significantly in the HPMG (all P<0.01), and the expressions of hypothalamic CRF and CRFR1 proteins and mRNAs decreased significantly (all P<0.05). There were no significant differences between the MG and the SHPMG (all P>0.05).

Conclusion

HPM can down-regulate the abnormally increased expressions of CRF and CRFR1 proteins and mRNAs in hypothalamus of the TNBS-induced experimental colitis rats, which is plausibly one of its action mechanisms in mitigating visceral pain and the pain-related emotions in the experimental colitis rats.

     

艾灸对克罗恩病大鼠结肠c-Jun 氨基末端激酶信号通路的影响

目的：通过观察艾灸对克罗恩病（Crohn??s Disease,CD）大鼠结肠c-Jun氨基末端激酶（c-Jun Nterminalkinase,JNK）、核转录因子c-Jun、单核细胞趋化因子1（Monocyte chemoattractant protein 1,MCP-1）、环氧合酶2（Cyclooxygenase 2,COX2）的调节作用,基于JNK信号通路探讨艾灸治疗CD的抗炎免疫机制。方法：清洁级雄性SD大鼠随机分为正常组、模型组、艾灸组和假灸组。采用三硝基苯磺酸（2,4,6 Trinitro-benzene-sulfonicacid,TNBS）合乙醇溶液灌肠制备CD模型。采用苏木素-伊红染色观察各组大鼠结肠组织形态学变化,并进行病理损伤评分;采用酶联免疫吸附测定法检测结肠组织MCP-1、COX2、JNK、c-Jun的含量;应用实时荧光定量PCR技术检测大鼠结肠JNK、c-Jun的mRNA表达。结果：与正常组比较,模型组大鼠结肠损伤严重,结肠损伤评分升高（P < 0.01）,主要表现为局灶性炎症,累及黏膜下层,裂隙状溃疡,部分可见肉芽肿形成;大鼠结肠炎性蛋白MCP-1、COX2 含量增加,JNK 蛋白含量和mRNA 表达均增加（均P < 0.05）,c-Jun 无明显变化（均P >0.05）。与模型组、假灸组比较,艾灸组大鼠结肠损伤评分降低（P < 0.01,P < 0.05）,结肠形态结构改善,炎症反应减轻,结肠MCP-1、COX2含量降低,JNK蛋白含量和mRNA表达均降低（均P < 0.05）,c-Jun无明显变化（均P > 0.05）。与模型组比较,假灸组大鼠各项指标均无显著变化（均P > 0.05）。结论：艾灸能下调CD大鼠结肠JNK蛋白和mRNA表达,降低结肠MCP-1、COX2含量,该作用可能是其减轻CD肠道炎症,促进结肠组织损伤修复的重要机制之一。     

隔药灸对克罗恩病大鼠结肠NLRP3炎症小体及IL-1?调节作用的实验研究

目的通过观察隔药灸对克罗恩病大鼠结肠NLRP3炎症小体(NLRP3、ASC、Caspase-1)及下游炎症因子IL-1?表达的影响,探讨隔药灸治疗克罗恩病的抗炎机制。方法将清洁级雄性SD大鼠随机分为正常组、模型组、隔药灸组和假灸组4组。采用三硝基苯磺酸(2,4,6-trinitrobenzenesulfonic acid, TNBS)与乙醇混合溶液灌肠制备大鼠克罗恩病模型。造模成功后,隔药灸组取天枢(双)、气海穴进行隔药饼灸治疗;假灸组取穴、操作同隔药灸组,但不点燃艾炷。治疗结束后,记录各组大鼠结肠长度及CMDI评分;采用HE染色,光学显微镜下观察各组大鼠结肠组织形态结构,并应用免疫组织化学技术检测各组大鼠结肠NLRP3、ASC、Caspase-1及IL-1?的表达。结果与正常组比较,模型组大鼠结肠组织损伤严重,表现为裂隙样溃疡,炎症细胞浸润伴水肿,部分大鼠结肠黏膜可见肉芽组织形成,且结肠NLRP3、ASC、Caspase-1、IL-1?蛋白表达显著增加(均P0.05);与模型组比较,隔药灸组大鼠结肠损伤有所修复,炎症反应减轻,结肠NLRP3、ASC、Caspase-1及IL-1?蛋白表达显著降低(均P0.05);假灸组大鼠结肠损伤程度、炎症反应与模型组类似,两组结肠NLRP3、ASC、Caspase-1、IL-1?表达比较,差异均无统计学意义(P0.05)。结论隔药灸能下调克罗恩病大鼠结肠NLRP3、ASC、Caspase-1及IL-1?的蛋白表达,促进结肠炎性损伤的修复。     

浅谈喉癌术后患者的呼吸道护理

喉癌是耳鼻咽喉科比较常见的恶性肿瘤,发病率约占全身恶性肿瘤的1%～5%,好发年龄为50～70岁,男性多于女性。随着喉癌手术技术日趋完善及医疗水平的提高,接受手术的患者逐年增加,因此,加强手术后护     

超声心动图在动脉导管未闭介入治疗中的应用

目的探讨超声心动图在Amplatzer堵闭器介入治疗动脉导管未闭（PDA）中的应用。方法堵闭术前超声观察PDA形态,测量其最小内径、肺动脉内径、左心腔、左室收缩功能指标。术后超声检测堵闭器位置、有无残余分流、肺动脉及降主动脉是否梗阻狭窄及肺动脉、左心腔等大小。结果与主动脉弓部左侧位造影比较,术前超声检测PDA形态、最小内径测量值等差异无统计学意义（P〉0．05）。15例患者中13例堵闭成功,术后即刻超声检查,10例无分流,3例少量分流。堵闭器位置均正常,降主动脉、肺动脉未见梗阻变窄,左心及肺动脉均较术前缩小。结论超声心动图在PDA堵闭术前病例选择、判断PDA大小形态、选择合适型号的堵闭器及术后疗效评价等方面具有重要的作用。     

正确的心理疏导对美容术后效果的影响

近年来,随着社会的不断发展和外科技术的不断提高,美容手术已被大众广泛地接受.但是美容外科的受术者往往存在许多心理问题,认识、理解并正确对待和解决心理问题,对于美容手术的成功与否至关重要.为此我们就正确的心理疏导对美容术后效果的影响进行了相应的研究.     

微创手术治疗腋臭的临床分析

目的探讨腋臭治疗的方法和疗效。方法采用腋下2～3个小切口,进行肿胀浸润麻醉,然后行破坏大汗腺刮吸术,术毕加压包扎。结果本组患者100例,90例切口Ⅰ期愈合,切口瘢痕轻微,10例出现皮下血肿,切口Ⅱ期愈合,无皮瓣坏死、感染等,随访6个月,臭味完全消除,腋毛稀少,有效率100％。结论微创手术治疗腋臭有效去除腋臭,具有疗效确切,瘢痕细小,对组织创伤小、恢复快,腋毛稀少等特点,术后无复发。     

NLRP3炎症小体与炎症性肠病的关系及针灸调节作用研究进展

炎症性肠病(inflammatory bowel disease,IBD)是一组慢性非特异性肠道炎症性疾病,包括溃疡性结肠炎(UC)和克罗恩病(CD)。炎症小体作为一种大分子复合蛋白,在炎症发生发展与机体固有免疫中发挥了重要作用。其中NLRP3在IBD的发生发展与维持肠道稳态的作用机制中起到了双向调节的作用。该文综述了NLRP3炎性小体在IBD中的研究进展以及针灸对其的调节作用及可能机制,以期为针灸治疗炎症性肠病的临床和机制研究提供新思路。