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目的:普查中国汉族人群HIV-1协同受体CCR5编码区的基因多态性位点,为中国的艾滋病防治提供依据。方法:CCR5编码区用2对引物进行PCR扩增,设计测序引物依次测序,样本数为45例,用DNAstar分析测序结果,寻找SNP位点。结果:在编码区共发现6个SNP位点,4个引起氨基酸改变:A184G、G503T、G668A、G999T;一个单碱基缺失,引起移码突变和提前终止。A184G、G503T、G999T,三个中国汉族人所特有的SNP位点为首次发现,等位基因频率分别为1%,39.5%和9.5%;其中G503T分布明显不符合Hardy-Weinberg平衡。G668A和894C缺失曾在中国和日本人群中发现过,但我们的结果与所报道的基因频率不完全一致。结论:中国汉族人CCR5编码区SNP位点有自己的特点,与高加索人和非洲人明显不同,与日本人也不完全一致。我们共找到5个引起氨基酸改变的突变或SNP位点,其中3个为首次发现,这些SNP对于HIV-1感染和艾滋病病程的影响值得进一步研究。 相似文献
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目的:调查中国汉族人群中HIV-1感染相关基因CCR5、CCR2b、CXCR4及SDF1编码区的基因多态性特点,为我国的艾滋病防治提供基础数据。方法:CCR5用2对引物进行PCR扩增,用PCR产物做模板直接测序。CCR2b编码区经PCR扩增后,用测序引物逐段分别测序。CXCR4(cDNA编号AF147204)编码区用2对引物进行PCR扩增,然后测序。SDF1编码区用4对引物进行PCR扩增,然后分别测序。样本总数为45例,测序结果用DNAstar综合分析,寻找和鉴定SNP位点。结果:CCR5基因编码区共发现6个SNP位点,4个引起氨基酸改变,1个单碱基缺失,引起移码突变和翻译提前终止。184A→G、503G→T、668G→A、999→T等位基因频率分别为1.1%、21.1%、8.9%和10.0%。CCR2b编码区共发现8个SNP位点,6个错义突变,即43位G→C、190位G→A、260-位C→A、302位C→A、315位G→C、433位G→A,突变频率分别为:30.0%、27.8%、32.2%、5.6%、10.0%和3.3%。CXCR4编码区共发现7个SNP位点,3个错义突变即38位C→T、90位A→T、712位A→C,1个终止突变:106位G→T,基因突变频率分别为:4.4%、4.4%、10.0%和3.3%。在SDF1编码区发现1个错义SNP位点:192位G→T,突变频率为8.9%;1例单碱基缺失:100位T缺失(100ΔT),引起34位氨基酸移码突变。结论:中国汉族人HIV-1相关基因编码区有自己的多态性特点。4个HIV-1相关基因编码区共工到22个SNP位点,17个为首次报道;2个单碱基缺失均导致移码突变和翻译提前终止,1个已经报道。它们对HIV-1感染和艾滋病病程的影响值得进一步研究。 相似文献
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目的:探讨奥美拉唑治疗胃溃疡合并出血的临床疗效。方法:选取本院2010年1月-2012年1月收治的胃溃疡患者132例,随机均分为治疗组和对照组。对照组接受常规治疗,治疗组接受奥美拉唑治疗,比较两组患者的临床治疗效果。结果:治疗组有效率为97.0%,对照组为84.8%。治疗组患者的治疗效果明显好于对照组,两组比较差异有统计学意义(P〈0.05)。治疗组满意度为98.5%,对照组为71.2%。治疗组患者满意度明显高于对照组,两组比较差异有统计学意义(P〈0.05)。治疗组患者的复发率明显小于对照组,两组比较差异有统计学意义(P〈0.05)。结论:奥美拉唑治疗患者胃溃疡合并出血症状疗效显著,患者满意度明显提高。 相似文献
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目的:调查中国汉族人群人类免疫缺陷病毒-1协同受体CC趋化因子受体-5[chemokine (CC)receptor 5,CCR5]编码区的基因多态性位点,为艾滋病的防治提供依据。方法:CCR5编码区用两对引物进行PCR扩增,设计测序引物依次测序,样本数为42份,用DNAstar分析测序结果,寻找单核苷酸多态性(single nucleotide polymorphism,SNP)位点。结果:在编码区共发现6个SNP位点,4个引起氨基酸改变:A184G、G503T、G668A、G999T;1个单碱基缺失,引起移码突变和提前终止。A184G、G503T、G999T3个中国汉族人所特有的SNP位点为首次发现,等位基因频率分别为1.2%,39.0%和9.5%;其中G503T分布明显不符合Hardy-Weinberg平衡。结论:中国汉族人CCR5编码区SNP位点有自己的特点,与高加索人和非洲人明显不同,与日本人也不完全一致。 相似文献
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目的 对中国汉族人群HIV—1协同受体CXCR4编码区的基因多态性进行研究,为中国的获得性免疫缺陷综合征(AIDS)防治提供依据。方法 CXCR4(cDNA编号AFl47204)编码区用2对引物进行PCR扩增,然后分别测序,测序样本数为48例。用DNAstar分析测序结果,寻找SNP位点。结果 在编码区发现了7个SNP位点,其中3个同义突变(129位C→T、426位C→T,968C→T),3个有义突变(38位C→T、90位A→T、712位A→C),1个终止突变(106位C→T使谷氨酸密码子变成终止密码子)。其中38位C→T、90位A→T、712位A→C、106位C→T,基因突变频率为4.2%、4.2%、9、4%和3.1%。结论 在CXCR4编码区找到的7个SNP位点中,4个引起氨基酸改变,1个已有报道,对HIV—1感染和AIDS病程的影响值得研究。 相似文献
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Genotypes and polymorphisms of mutant CCR5-△32,CCR2-64I and SDF1-3’A HIV- 1 resistance alleles in indigenous Han Chinese 总被引:3,自引:0,他引:3
Objective To understand the interaction between surfactant proteins and pneumocys tis carinii pneumonia (PCP), and the impact of corticosteriods on surfactant proteins.Methods We established rat models of PCP and bacterial pneumonia induced by subcutaneous injection of 25mg cortisone acetate. At 8-12 wk, the bronchoalveolar lavage f luid (BALF) of rats was collected. Total nucleated cells of BALF were counted a nd differentiated, and the concentrations of surfactant protein A (SP- A) and su rfactant protein D (SP- D) were measured by immunoblotting assay. The rats were divided into three immunosuppressive groups and a normal control group. Group Ⅰ, normal control (n=6), consisted of healthy SD rats; group Ⅱ, negative cont rol (n=6), consisted of rats with cortisone acetate injection for over 8 wk with out lung infection; group Ⅲ, bacterial pneumonia (n=11), rats were injected wit h cortisone acetate over 8 wk that resulted in bacterial pneumonia without other pathogens isolated; and group Ⅳ, PCP (n=14), rats with injected cortisone acet ate for 8-12 wk and developed PCP without other pathogens isolated. Results Our results indicated that the total cell count in BALF in the negative control group was lower than that in the normal control group (P<0.001). During P CP infection, the total cell count and the percentage of polymorphonuclearcytes (PMNs) in BALF were signi ficantly increased (P<0.01), but were lower than those in the bacterial pne umonia group. The concentration of SP- A of BALF in PCP (45.1±22.1 μg/ml) was significantly increased in comparison with that in the negative control ( 16.2±9.9 μg/ml, P<0.05) and bacterial pneumonia groups (6.2±5.6 μ g/ml, P<0.001). We also found that the relative content of SP - D was signi ficantly higher in PCP (24 249±4780 grey values) than that in the negative control (13 384±2887 grey values, P<0.001) and that in bacterial pne u monia (11 989±2750 grey values, P<0.001). SP- A and SP- D were a lso higher in the moderate to heavy group of PCP than those seen in the mild group (P<0.01, P<0.001). SP- A and SP- D were higher in the negative contr ol group than those in the normal control group, but there was no significant di fference between the 2 groups. Conclusion These results suggest that the concentrations of SP- A and SP- D in BALF are inc reased by pneumocystis carinii specific stimulation, but the alteration is n ot related to the corticosteriod usage. 相似文献
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核酶是一类具有生物催化活性的RNA分子。它能够定点切割mRNA靶分子,从而有效阻断基因表达。因此,核酶不但是研究肿瘤发病机制的有用工具,它在抗肿瘤增殖、转移、多药耐药的基因治疗中也具有潜在的应用价值。 相似文献
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左旋肉碱对内毒素致肝损伤的保护作用 总被引:3,自引:0,他引:3
在慢性肝病发病机理的研究中,内毒素在肝损伤过程中的作用一直受到关注。慢性肝炎的反复发作和重型化,以及与之相关的一系列并发症如肝脏循环障碍、黄疸、出血、腹水、肝肾综合症等,均与内毒素有密切关系。近年来发现,一种与脂肪酸代谢有关的营养素,左旋肉碱(L-c... 相似文献